• Journal of Bio-Environment Control
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• v.15 no.4
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• pp.327-334
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• 2006

In tomato perlite bag culture, the available water content was analyzed for several sorts of perlite so that the irrigation safety was investigated and thus the irrigation strategy controlled by time clock was established. The reduction trends of water content in perlite bags were monitored for tomatoes, of which fruits were harvested until the fifth cluster. The amount of daily reduction of water in the bag was on the decrease as the total water in the bag was decreased. In terms of time interval from when the water content based on weight was reduced more than 50g to when it was dropped again, the longest time interval in a day was retarded gradually. It means plant activity was recovered later than the previous day. The available water content in perlite bag of 40 liters was about 30% which was 12 kg in weight, which satisfied daily water demand of 6 tomato plants. The appropriate time irrigated by time clock was recommended for the case that it was irrigated 5 or 10 times a day with the daily integrated solar radiation of 601 or $1,519W/m^2$.

• Applied Biological Chemistry
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• v.42 no.3
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• pp.229-234
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• 1999

The effects of linoleic acid(LA, 18 : 2) and/or bovine serum albumin(BSA) on the lipid metabolism in human hepatoma cell line HepG2 cells were evaluated. HepG2 cells were cultured in basal Dulbecco's modified Eagle's(DME) medium(Basal medium), DME medium containing 0.2 mM LA(LA medium), or DME medium containing both 0.2 mM LA and 0.2-1.0% BSA(LA+BSA medium). $[^{14}C]Acetate(0.3\;{\mu}Ci/ml\;medium)$ was added as a radioactive lipid precursor and the cells were incubated for 6 hours. An addition of LA to basal medium resulted in a decrease in the incorporation of $[^{14}C]acetate$ into total cholesterol fraction. In contrast, an addition of BSA to LA-containing medium tended to increase the incorporation of $[^{14}C]acetate$ into total cholesterol. The alteration of cholesterol metabolism in HepG2 cells incubated in LA+BSA medium was attributed by an increase in the incorporation of $[^{14}C]acetate$ into free cholesterol, but not cholesteryl ester fraction. In addition, the secretion of cholesterol was increased by LA+BSA medium, suggesting that BSA stimulates cholesterol secretion. No significant change in the incorporation of $[^{14}C]acetate$ into cellular total lipids was observed among the experimental groups. However, an increased incorporation of $[^{14}C]-labelled$ fatty acid into cellular triacylglycerol and decreased incorporation into phospholipid were observed in cells incubated with LA+BSA medium as compared to those of LA medium. The secretions of $[^{14}C]-labelled$ triacylglycerol, phospholipid, and free fatty acid were also stimulated in HepG2 cells incubated with LA+BSA medium. In conclusion, the present study suggests that in human hepatocytes, LA and BSA influence lipid metabolism, and BSA enhances the secretion of lipids.

• Journal of Korean Society of Forest Science
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• v.35 no.1
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• pp.56-60
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• 1977

This experiment was conducted to determine the responses of some pine species to acidity of cultured soils, and absorption behavior of alkaline elements and to observe the growth characteristics during afforesting period. The results were summarized as follows. 1. Total dry weight indicating growth volume was not much influenced by the acidity of cultured soils (pH 4~8) in all of the 4 species studied. Pinus koraiensis showed more dry weight than any other species, which indicated statistically significant difference. 2. T-R ratio of dry weight was not much affected by the acidity of cultured soils (pH 4~8) and the difference of cultured soils (pH 4~8) and by species. Growth phase also did not show remarkable change through the experiment period. 3. The average sodium content ratio in the plant body had no correlation with cultured soils (pH 4~8), bue significant differences were recognized among species statistically. 4. It was found that sodium content ratio was not much influenced by the cultured soils (pH 4~8), even when the tops and roots were separately analyzed. But, very significant difference was found among the species. Pinus koraiensis especially, showed greater volume growth maintaining low sodium content ratio than any other species, which indicates that Pinus koraiensis has unfavorable characteristics for growing on the seaside.

• The Korean Journal of Mycology
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• v.15 no.3
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• pp.149-157
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• 1987

This study was undertaken to investigate the differentiation, from spore germination to hyphae growth and phialide formation, of Aspergillus niger through the method of synchronous and submerged culture. Through continuous experiments by shake culture with potassium acetate medium, we observed the formation of spores at appropriate concentration and pH. Potassium acetate medium was set pH 5.5, 6.0, 6.5 and 7.0 on each scale, and control, 20 mM, and 40 mM, 80 mM and 160 mM concentrations on the other scale. Aspergillus niger was cultured in the defined media at $28^{\circ}C$, and mycelial dry weight, changes of pH and the onset of sporulation were checked. The mycelial dry weight, increased in potassium acetate medium, and pH increased during mycelial growth and gradually decreased after the spore formation. When pH increased excessively in Potassium acetate medium with pH 7.0, the mycelia could not adapt and mycelial dry weight decreased gradually. At pH 5.5, the onset of sporulation was done within one day at 20 mM it took, at 80 mM three days and at 160 mM concentration. in two days, at 40 mM one to four days were taken, 80 mM concentration respectively. At pH 6.5, the onset of sporulation was done in three days and four days at 80 mM concentrations respectively. Spore formation was not shown at pH 7.0. In controlled medium with all levels of pH, spore formation was not shown.

• Journal of Mushroom
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• v.16 no.2
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• pp.70-73
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• 2018

To determine the optimum amount of spent oyster mushroom substrate (SOMS) for use in cultivation of Lentinula edodes, the chemical properties of the substrate and culture conditions of Lentinula edodes were investigated. Replacing 20-50% of a sawdust substrate with SOMS yielded a C/N ratio of 62-76. In case of substrates containing SOMS, the total nitrogen and phenolic contents of were higher, whereas fructose and organic acid contents were lower than those of the control substrate. Cultivation tests showed that the 3-cycle yield of 20% SOMS treatment was 286.7 g, similar to that of the control, while 50% SOMS treatment significantly decreased the yield. In conclusion, development of oak mushroom substrate using SOMS would recycle waste products and decrease material costs.

• The Korean Journal of Mycology
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• v.30 no.1
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• pp.11-17
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• 2002

This experiment was carried out to study formation of fruitbody with Cordyceps scarabaeicola (EFCC C-252) isolate. This isolate was the one of best fruitbody formation on brown rice 60 g plus 30 g silkworm pupa media among EFCC C-251, EFCC C-252, EFCC C-1092 from EFCC (Entomopathogenic Fungal Culture Collection) of Kangwon National University. Fruiting body was formed only isolate EFCC C-252 among tested isolates on the medium of brown rice (60 g) and silkworm pupae (30 g). The optimal temperature and light for fruiting body formation were $25^{\circ}C$ and fluorescent light (300 lux). The maximal fruiting body formation was observed at 70 g of brown rice and 80 g of silkworm pupa medium which was treated separately. Fruiting body was formed maximally by 2 days interval of irrigation.

• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.316-325
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• 2009

Adequate conditions of water content and aeration of container media are major environmental factors in the production of pot plant. This experiment was carried out to find optimum physical property of media for the production of small potted Ardisia in capillary mat irrigation system. The plant materials used in this experiment were Ardisia pusilla and Ardisia japonica. Seven substrates were formulated by blending perlite or fresh rice hulls at 20%, 40%, 60% (v/v) with sphagnum peat. Total pore space (TPS) increased by blending sphagnum peat with fresh rice hulls, but decreased by blending sphagnum peat with perlite. As fresh rice hull (FRH) and perlite content increased, air filled pore space (AFP) of substrate increased but container capacity (CC) decreased. Substrate blended with fresh rice hull was higher AFP than blended with perlite and the rate of increase was higher for FRH-containing substrate. As AFP increased, the $CO_2$ concentration in the pot decreased and the $CO_2$ concentration of substrate blended with FRH was higher than blended with perlite. The fresh and dry weight of Ardisia pusilla and A. japonica was the highest in the substrate contained 60% FRH, but the ratio of shoot dry weight to root dry weight was the lowest. The optimum total pore space, air-filled pore space, water holding capacity of substrate for the growth of Ardisia pusilla and A. japonica in the capillary mat irrigation system were 82.8%, 25.6%, and 57.2% respectively.

• Journal of Bio-Environment Control
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• v.17 no.3
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• pp.215-220
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• 2008

This study was carried out to find optimum accumulative solar radiation in 'Carnidal' of Perlite Media. The pH was stable from 6.0 to 6.7 during cultivation. Electric conductivity by drainage was higher water than by supply water and electric conductivity was increased at later. Inorganic compound, such as phosphoric, kalium and magnesium were accumulated in crossed system. Especially, kalium and magnesium were highly accumulated. When solar radiation high, consumption of the amount of nutrient solution were increased. Sap flew was $273g{\cdot}hr^{-1}$ per hour from 10 : 30 to 11 : 00 AM. However there was no relation-ship between solar radiation and the mount of sap flew. When amount of solar radiation was $250W{\cdot}hr^{-1}$, cut rose 'Cardinal' nutrient consumption was 212.8 mL at nutrient supply of 50 mL. The yield of cut rose 'Cardinal' was 154.6 ea/10a in perlite media. In the cut rose 'Cardinal', nutrient solution was 50 mL as supplied at solar radiation of $200W{\cdot}hr^{-1}$ and nutrient solution was 30mL as supplied at solar radiation of $250W{\cdot}hr^{-1}$ at low solar radiation in perlite.

• Microbiology and Biotechnology Letters
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• v.7 no.1
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• pp.37-46
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• 1979

In order to investigate the compositional change of the lauan and pine sawdust media during the coltivation of oyster mushroom (Pleurotus ostreatus) chemical components of the two media and mushroom were analized periodically from spawning to ending. The results are summerized as follows: 1) The total yield of mushroom from the lauan sawdust media was 6.39 kg/$m^2$, and from the Pine saw-dust media 6.31 kg/$m^2$, 94％ of which was produced from the first and second cropping period. 2) In both media ash content increased gradually, while organic matter content decreased as the cultivation progressed. Moisture content, which was about 79％ at the time of spawning, decreased a little during the priod of mycelial propagation, after which no change was observed. 3) Total nitrogen content of the two media decreased gradually as the cultivation progressed, and total loss of insoluble nitrogen was greater than that of soluble nitrogen. Amino nitrogen content tended to increase continually. 4) In both media pentosan, $\alpha$-cellulose, lignin and C/N ratio decreased gradually, while the content of reduced sugar, trehalose and mannitol continued to increase. 5) In both media phosphorus, potassium, manganese and zinc decreased, and magnecium, calcium, iron and copper showed irregular changes. 6) CMC-saccharifying and liquefying activity gradually increased from after mycelial propagation to the second cropping, after which it decreased in both media. Xylanase activity rapidly and greatly increased during the second cropping period rather than the first period. At the start of the third cropping period the activity decreased slowly. Protease activity was highest after mycelial propagati, after which it gradually decreased. The pH of the two media decreased as the cultivation progressed. 7) In both media little change was observed in the content of the components of mushroom cropped from the first to the third period, but slight decrease was noticed at the fourth cropping.

• Korean Journal of Plant Resources
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• v.22 no.4
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• pp.337-342
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• 2009

Present studies were conducted to evaluate the effects of medium strength(MS and Hyponex), carbon sources and their concentrations, agar concentrations, and inoculation amounts on prothallus propagation of Pterdium aquilinum var. latiusculum(Desv.) Underw. ex Hell in vitro. The optimum MS medium strength for prothallus propagation was 2MS concentration. Phosphate source was most effective for prothallus growth of P. aquilinum var. latisculum. The addition of 1% sucrose or glucose to MS medium promoted prothallus multiplication. Growth of prothallus was not affected by agar concentration. Propagation of homogenized prothallus was vigorous even in liquid medium. Chopped gametophytes(100 and 200 mg) were inoculated on 250 ml ${\Delta}$flask with 100 mL of 2MS concentration medium and suspension culture was done at 100 rpm for 22 days. After 20 days, prothallus multiplication slowed down, so 100 mg of chopped prothalli is recommended for initial inoculation, since initial amount of inoculum did not affect subsequent prothallus multiplication. Consequently after 20 days of suspension culture, prothallus should be subcultured or transplanted outside of growing vessels.