• Title/Summary/Keyword: 배지량

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Effect of Indole on the Cell Growth and Synthesis of Indirubin in Suspension Culture of Polygonum tinctorium LOUR (쪽 현탁배양(懸濁培養)에서 Indole이 세포 생장과 Indirubin 생성에 미치는 영향)

  • Chung, Eun-Suk;Chae, Young-Am
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.2
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    • pp.96-99
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    • 1995
  • This experiment was carried out to analyze the effect of indole on the synthesis of indirubin in suspension culture of Polygonum tinctorium. Adding indole and L-tryptophan into culture media was re­vealed that indirubin was synthesized in callus grown on solid medium containing indole and proper concentration of indole for indirubin production was decided as 200mg/1. Indirubin content in suspension culture was higher than in solid medium with considerable amount of indirubin secresed into media in suspension culture and highest quantity of indirubin was obtained when indole was added into medium after 20 days suspension culture.

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Determination of Proper Amounts of Media and Effects of Fermented Sawdust Addition on Yields of Grifola frondosa in the Bag Culture (잎새버섯 봉지재배시 적정 배지량 및 발효톱밥 첨가효과)

  • Lee, Jae-Hong;Mun, Yun-Gi;Jeong, Tae-Sung;Choi, Jun-Keun;Park, Young-Hak;Kim, Kyung-Hee;Yu, Young-Bok
    • Journal of Mushroom
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    • v.10 no.4
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    • pp.179-183
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    • 2012
  • This study was conducted to determine proper amount of media and the effect of fermented sawdust on yield of Grifola frondosa in bag culture. The yield of Grifola frondosa was 227 g for 2 kg of media compared with 103 g for 1kg, 256 g for 2.5 kg, and 312 g for 3 kg. The greatest yield of Grifola frondosa per mass of media was 114 g/kg media for 2 kg of media. Days to harvest for 2 kg of media was 95 days compared with 93 days for 1kg, 99 days for 2.5kg, and 104 days for 3kg. Addition of fermented oak sawdust increased yield from 90 to 145 g/kg bag for Hambak cultivar and from 104 to 121 g/kg bag for Yipsae1ho cultivar. Culture duration was shorten 4-7 days by treating fermented oak sawdust.

Effect of Air Humidity and Water Content of Medium on the Growth and Physiological Disorder of Paprika in Summer Hydroponics (여름철 착색단고추 수경재배 시 공중습도 및 배지함수량이 생육 및 과실의 생리장해에 미치는 영향)

  • Rhee, Han-Cheol;Seo, Tae-Cheol;Choi, Gyoeng-Lee;Roh, Mi-Young;Cho, Myeung-Whan
    • Journal of Bio-Environment Control
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    • v.19 no.4
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    • pp.305-310
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    • 2010
  • This study was carried out to investigate the effect of air humidity and water content of medium on the growth and physiological disorder of paprika in summer hydroponics. Treatments were composed of air humidity of control (over 90%) and dehumidification (low 90%) and water content of 80% and 50%. Time domain reflectometry (TDR) sensors and dehumidifier were used in a drip irrigation system and control system of air humidity, respectively. The early growth of paprika was not affected by air humidity but increased by high water content (80%) of medium. Mean fruit weight was reduced at high air humidity and low water content (50%) of medium, but the fruit number per plant and yield were increased at low air humidity. The incidence of brown fruit stem increased with increasing air humidity and water content of medium. Rate of blossom end rot increased in the low water content medium compared with the high water content medium. The nitrogen (N) was higher content in brown fruit stem than normal stem, but calcium (Ca) was lower.

Growth and Pigment Formation of Genus Monascus on Medium Compositions (배지에 따른 Genus Monascus의 증식도와 색소함량)

  • 윤은경;김미정;김순동
    • Food Science and Preservation
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    • v.9 no.4
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    • pp.425-428
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    • 2002
  • Growth and pigment formation in Genus Monascus(M. pilosus and M purpureus) related 15 kinds of culture media(Lin medium, SP medium, YM medium, YE medium, GMIN medium, SMO medium, MY medium, GY medium, Nishikawa medium, sucrose medium, stock culture, Mizutani medium, modified Lin medium, Toya medium and rice medium) were investigated. Mizutani medium and Lin medium among 15 kinds of the culture media showed good growth for M. pilosus, M purpureus, fresh mycelium weight cultivated for 10 days at 30$^{\circ}C$ was each 24.5∼26.9 or 15.9∼17.2 g/100 mL. The culture media which showed higher content of pigment production in two fungi were Lin medium(OD: 1.2 ∼ 1.6 and Mizutani medium(OD: 0.8 ∼ 1.0) that showed higher in M. pilosus.

Growth and Rotting Characteristics of Lentinus edodes Isolates in Korea and Their Adaptability to Bag-Culture Using Oak Sawdust (우리나라 표고의 생장(生長), 부후특성(腐朽特性) 및 톱밥배양(培養)에 관(關)한 연구(硏究))

  • Bak, Won Chull;Lee, Eun Young;Yoon, Kab He;Lee, Won Kyu;Yi, Chang Keun;Hong, Ki Sung
    • Journal of Korean Society of Forest Science
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    • v.83 no.1
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    • pp.12-19
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    • 1994
  • Studies were made to find out the mycelial growth and rotting characteristics of 91 Lentinus edodes isolates collected or hybridized in Korea and abroad, and their adaptability to bag-culture. The results are as follows. 1. There were little differences in mycelial growth at PDA and oak (Quercus mongolica) sawdust-based medium, and rotting among high-, mid- and low temperature types of L. edodes isolates. But, the fruit yield of high-temperature type was much higher than those of the other two types. The fruit yields of the isolates in the same strain group were apparently different. 2. From the sawdust-based culture of 91 isolates, FRI 221, FRI 208 and FRI 169 were selected as excellent strains in yield and Quality, showing fruiting of 157g, 152g and 119g per 1kg-medium, respectively. 3. Attempts to culture in various media with different substrates resulted in almost same fruit yields, and the yield was proportional to medium weights as 2.5kg-medium showed 2.5 times more yield than 1kg-medium. Fruit yields were different according to incubation periods and the period of little more than 100days was best. 4. When the correlation analyses among mycelial growth, rotting ability, yield and fruit-shape normality were made with the 91 isolates, mycelial growth at sawdust-based medium showed highly positive correlation with that at PDA, while fruit yield was negatively correlated with mycelial growth in PDA and sawdust-based medium.

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Maximization of cell growth and polysaccharide production from Agaricus blazei by fed-batch cultivation

  • Hwang, Jeong-Min;Seo, Jeong-Sik;Gwon, Myeong-Sang;Choe, Jeong-U;Han, Jin-Su;Hong, Eok-Gi
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.283-286
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    • 2000
  • In order to maximize the cell growth and the polysaccharide production in Agaricus blazei, two kinds of fed-batch fermentation processes were performed with varying the feeding medium compositions and the feeding process. The relationship between dissolved oxygen and polysaccharide production in batch fermentation was applied to fed-batch fermentation. The biomasss concentration was 18.2 g/L and the polysaccharide production was 10.4 g/L.

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Study on the Production of Organic Acid by Fermentation with Mixed Culture of Lactobacillus acidophilus KCCM 32820 and Propionibacterium freudenreichii KCCM 31227 in Whey Broth (유청배지에 Lactobacillus acidophilus KCCM 32820과 Propionibacterium freudenreichii KCCM 31227로 혼합배양시 유기산 생성에 관한 연구)

  • Lee, Jeong-Hoon
    • Culinary science and hospitality research
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    • v.13 no.1 s.32
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    • pp.55-61
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    • 2007
  • This study was carried out to evaluate the growth characteristics of Lactobacillus acidophilus KCCM 32820 and Propionibacterium freudenreichii KCCM 31227 and the production of propionic and acetic acids in 5% and 10% whey broth by mixed culture of L. acidophilus KCCM 32820 and P. freudenreichii KCCM 31227. Exponential phase of L. acidophilus KCCM 32820 was in the range of $6\sim12$ hrs and P. freudenreichii KCCM 31227 was in the range of $36\sim108$ hrs. In the mixed culture, production of propionic acid was shown to be greater value in the 10% whey broth than in the 5% whey broth and to be greater value in the low temperature for a long time than in sterilization by autoclave. Maximum production of propionic acid was 8.88 mg/mL in the 10% whey broth fermented at 120hrs. Production of acetic acid was revealed to be greater value in the 10% whey broth than in the 5% whey broth. The production quantity ratio of propionic acid to acetic acid was shown between $2:1\sim3:1$ during the fermentation process.

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Feeding rate and growth rate of earthworm(Oligochaeta : Eisenia fetida) population on the spent substrate of the agaric-mushroom cultivation (느타리버섯 폐배지에 대한 줄지렁이(Eisenia fetida) 개체군의 섭식률 및 생장률)

  • Bae, Yun-Hwan;Yang, Yong-Un
    • Journal of the Korea Organic Resources Recycling Association
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    • v.18 no.4
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    • pp.45-53
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    • 2010
  • Feeding rate and growth rate of earthworm population on the variously pretreated spent materials of the agaric-mushroom cultivation were investigated. When the spent mushroom substrates with different aging periods were supplied to earthworm, feeding rate and growth rate of earthworm population on spent mushroom substrates aged less than 10 days were higher than that on spent mushroom substrates aged more than 20 days. Feeding rate and growth rate were not increased when the spent mushroom substrate mixed with vermicasts or nitrogenous fertilizer was supplied. Feeding rate and growth rate on the ground mushroom substrate were higher than that on the non-ground mushroom substrate. Especially when the ground mushroom substrate was mixed with rice bran and supplied to earthworms, growth rate was much higher than that on the non-ground spent mushroom substrate; it increased 1.85 times.

Comparison of Shiitake Productivity in Sawdust Media According to the use of Sawdust, Plug-shaped and Liquid Spawn (톱밥종균, 성형종균 및 액체종균을 사용한 표고톱밥배지에서의 생산성 비교)

  • Lee, Bong-Hun;Bak, Won-Chull;Yoon, Kab-Hee
    • The Korean Journal of Mycology
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    • v.34 no.2
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    • pp.79-83
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    • 2006
  • This study was made to investigate the difference of the shiitake productivity according to the use of sawdust, plug-shaped and liquid spawn. Contamination of medium was confirmed by eye. There was no contamination in the liquid spawn treatment. And, contamination rates in the sawdust spawn and plug-shaped spawn were 6.7%, respectively. Productivity of fruit-bodies was 725.1 g from sawdust spawn, 726.2 g from plug-shaped spawn and 573.9 g from liquid spawn until 3rd flushing of fruit-bodies. The number of fruit-bodies was 57.4 per medium of sawdust spawn, 64.3 plug-shaped spawn and 45.1 liquid spawn. At yield per medium, the percentage of media producing over 1,000 g was 13% in plug-shaped spawn and 7% in liquid spawn. However, there was no medium producing over 1,000 g of fruit-bodies in sawdust spawn. Fruit-bodies of $600{\sim}900g$ were yielded from 79% of sawdust spawn treatment, 53% plug-shaped spawn and 33% liquid spawn treatment. When compared the productivity of fruit-bodies, liquid spawn treatment appeared lower than sawdust spawn and plug-shaped spawn treatment at 1st flushing, but appeared higher than the others at 2nd and 3rd flushing.

Culture Condition and Inoculum Volume of Liquid Spawn on the Bottled Cultivation of Agrocybe cylindracea (버들송이(Agrocybe cylindracea)의 액체종균 배양조건과 접종량)

  • Cheong, Jong-Chun;Hong, In-Pyo;Jang, Kab-Yeul;Park, Jeong-Sik
    • The Korean Journal of Mycology
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    • v.31 no.2
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    • pp.94-97
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    • 2003
  • In order to shorten cultivation periods of Agrocybe cylindracea, liquid spawn was used in place of sawdust spawn. Optimum culture condition of liquid spawn was PSB (potato extract sugar broth) medium, $25^{\circ}C$ temperature and pH 7.0. And, optimum inoculum volume was $10{\sim}15ml$. Two grams per liter of dry weight of the fungal mycelia were obtained with 10l of culture bottle with liquid spawn after 10 days. In 850 ml-bottle of sawdust media, cultivation period using liquid spawn (30 days) was five days shorter compared with the cultivation period in sawdust spawn (35 days). Also, complete growth of sawdust media was increased to 92% (liquid spawn) from 75% (sawdust spawn) and yield of fruiting bodies was increased to about 10%.