• Journal of Bio-Environment Control
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• 제30권1호
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• pp.27-36
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• 2021

A capacitive water sensor was developed to measure the capacitance over a wide part of a substrate using an insulated electrode plate (30 cm × 10 cm) with copper and Teflon attached on either side of the substrate. This study aimed to convert the capacitance output obtained from the condenser-type capacitance sensor into the substrate water content. The quantification experiment was performed by measuring the changes in substrate water weight and capacitance while providing a nutrient solution and by subsequently comparing these values. The substrate water weight and capacitance were measured every 20 to 30 seconds using the sensor and load cell with a software developed specifically for this study. Using a curve-fitting program, the substrate water content was estimated from the output of the capacitance using the water weight and capacitance of the substrate as variables. When the amount of water supplied was increased, the capacitance tended to increase. Coefficient of variation (CV) in capacitance according to the water weight in substrate was greater with the 1.0 kg of water weight, compared with other weights. Thus, the fitting was performed with higher than 1.0 kg, from 1.7 to 6.0 kg of water weight. The correlation coefficient between the capacitance and water weight in substrate was 0.9696. The calibration equation estimated water content from the capacitance, and it was compared with the substrate water weight measured by the load cell.

• Proceedings of the Korea Water Resources Association Conference
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• 한국수자원학회 2012년도 학술발표회
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• pp.448-448
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• 2012

농경지의 잔류비료나 농약 등의 비점오염원은 강수 또는 관개를 함에 따라 지표 및 지하 유출과 함께 거동함으로써 토양 내에 집적됨과 동시에 지하수 수질에도 영향을 미칠 수 있다. 특히, 최근 고부가가치 농산물 수요의 증가로 전국적으로 확대되고 있는 시설재배의 경우 노지재배와는 상이한 유출 및 침투 특성을 가지고 있음과 동시에 과다시비로 인한 오염원의 토양 내 집적이 심각한 수준이나, 이에 대한 연구가 미흡한 실정이다. 따라서 본 연구에서는 시설재배지와 관행재배 지역의 토양 내 비점오염원 오염부하량을 평가하고 지하침투 과정을 평가하기 위하여 시설재배지와 관행재배지의 비교 평가가 가능한 포장 및 광역단위 시험포장을 연구대상지역으로 선정하였다. 모니터링 대상지역에서 토양수, 토양수분, 관개량, 기상조건 등을 측정하기 위한 장비를 설치하여 비점 모니터링 시스템을 구축하였고, 한달에 두 번 모니터링을 통하여 자료 수집 체계를 확립하였다. 시설재배지 및 관행재배지 토양 및 토양수 수질 분석 결과, 시설재배지 내 영양물질 농도가 관행재배지와 비교하여 전반적으로 높았으며, 특히, 하부토층으로 내려갈수록 영양물질의 농도 및 편차가 크게 나타났다. 향후 본 시험포장에서의 지속적인 모니터링 및 샘플링을 통하여 시설재배지 토양내 비점오염원의 침투 과정을 분석할 수 있을 것으로 판단되며, 지하침투 영향 및 오염부하량 모델링 기초자료로 활용될 수 있을 것으로 판단된다.

• Journal of Mushroom
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• 제12권4호
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• pp.350-356
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• 2014

This study was conducted to evaluate the feeding value of the spent mushroom (Hypsizygus marmoreus) substrates (SMS) in laying hens (Hy-Line Brown). The fresh spent mushroom (Hypsizygus marmoreus) substrates collected from the Namkyung farm were fermented with Bacillus subtilis EJ3 for 2 weeks. A total of twenty-four laying hens were fed corn-soy based experimental diets containing 0% (T0), 5% (T1), 10% (T2) and 15%(T3) fermented SMS for 12 weeks. There were no significant differences among the treatments in egg production, egg weight, egg mass, feed conversion and viability during the experimental period. Feed intake was significantly lowered in T0 (118.3 g) than T1 (121.9 g), T2 (120.3 g) and T3 (122.4 g). There were no significant differences among the treatments eggshell breaking strength, thickness and haugh unit, whereas the yolk color of T1, T2 and T3 were significantly heavy than T0. The palatability of boiled meat was significantly better in the T3 laying hens than in the T0 laying hens. In conclusion, fermented SMS can be used as resource of feed in laying hen feed at 5.0-15% level without effect on performance and egg qualify.

• Journal of Life Science
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• 제14권4호
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• pp.560-566
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• 2004

Agrocybe aegerita is Hymenomycetes fungus belonging to the order Agaricales and family of Bolbitiaceae. Much less is known about liquid culture of Agrocybe aegerita. Thus, the present study was to investigate the liquid cultural characteristics of Agrocybe aegerita my-celium. The optimal medium for the mycelial growth and density was ME medium, optimal tem-perature and initial pH were 25$\pm$1$^{\circ}C$ and 5.5, respectively. And optimal culture time for mycelial growth was 12 days. The modified optimal medium compositions were dextrin 3% (w/v), yeast extract 2% (w/w), MgSO$_4$0.05% (w/v), and KH$_2$PO$_4$0.15% (w/v). Under optimal culture conditions, the mycelial growth of modified optimal medium was higher than that of ME medium.

• Journal of Bio-Environment Control
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• 제21권3호
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• pp.236-242
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• 2012

This study was carried out to investigate the effect the water content in coir substrates as according to growth stage on the growth and yield of mini-paprika of three varieties 'E 499524' (red color), 'E 499526' (yellow colar) and 'E 499531' (orange colar) in summer hydroponics. Treatments of I, II and III were composed of 55-65-60, 50-60-55 and 45-55-50% in water contents of growth stages, respectively. Time domain reflectometry (TDR) sensors were used in a drip irrigation system. The early growth of mini-paprika was increased by high medium water content of treatment I in all of three varieties. Mean fruit weight was not affected by medium water content, but the fruit number per plant and yield were increased at high medium water content of treatment I. The Yield of treatment I was higher than that of treatment II and III in all of three varieties. The sugar content was increased by low medium water content of treatment in all of three varieties. The incidence of brown-stem fruit, blossom-end rot and sunburn was decreased with increasing water content of medium. Occurred in the low water content of medium. Mineral contents of fruits such as nitrogen (N), potassium (K), calcium (Ca), magnesium (Mg) etc. were not affected in all of three varieties.

• Journal of Animal Science and Technology
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• 제49권1호
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• pp.79-88
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• 2007

This study was conducted to determine the minimal yield of spent mushroom substrates (SMS) and physicochemical characteristics of Pleurotus ryngii, Pleurotus osteratus and Ammulina velutipes according to mushroom species and cultivation types. The annual yield of SMS in 2004 was minimally 1,670,182M/T and 10.7-fold of the mushroom yield. The yield of SMS for Pleurotus ryngii, Pleurotus osteratus and Ammulina velutipes was 972,141M/T and was 58.2% of the total yield of SMS. Data from the chemical analysis of totally 109 SMS samples revealed that bed type cultivation showed low NDF (65.2%), high NFC (12.7%) and high ash (11.5%) contents (P<0.05), resulting in better nutritional characteristics compared with bottle or vinyl bag cultivation. In general, it was more desirable to classify SMS by cultivation types rather than by mushroom species for the effective use of SMS as an animal feed. Among cultivation types, SMS from bed type cultivation needed to be preferably used as feed.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 한국생물환경조절학회 1998년도 정기총회 및 학술논문 발표요지
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• pp.122-127
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• 1998

(1) 급액천의 규격별 양액공급량 $\bigcirc$ 합섬(비스코스＋레이온) : 시간당 소(12.6ml), 중(21.2ml), 대(57.5ml)의 순으로 유입되었으며 중과 대의 급액천의 유입은 거의 같아 급액천의 규격간에 공급량은 비례하지 않았다. $\bigcirc$ 합섬(폴리에스텔) : 소(4.3ml), 중(20ml), 대(57.5ml)순으로 양액이 유출되었으며, $\bigcirc$ 합섬(인견) : 소(2.9ml), 중(23.7ml), 대(32.3ml)순으로 유입되었으나 소(급액천)의 유출량은 적어 급액천 재질로서 부적합하다고 생각되었으며, $\bigcirc$ 실(cotton) : 소(0.2ml), 중(3.0ml), 대(2.7ml)순으로 규격간에 유입은 차이가 없었으며 전체적으로 유출이 적어 급액천으로서는 부적합하였다. (2) 배지의 규격(종류)별 양액의 유입량 $\bigcirc$ 송이(Ф6.0mm~12.0mm이상) : 배지의 입자가 굵은 배지일수록 급액초기 1~2시간이 유입이 급격히 증가하였으나 시간이 경과할수록 일정한 량을 보였으며 유입량은 다른 배지에 비하여 많았고 급액천의 규격이 클수록 유입되는 량이 많았으나 시간이 경과함에 따라 감소하는 경향을 보였다. $\bigcirc$ 송이(Ф1~5mm) : 합섬A(비스코스+레이온)과 합섬B(폴리에스텔)에서는 시간당 15.0~50.1ml가 유입되었으나 합섬C(인견)과 실에서는 시간당 10ml이하로 토출량이 적었다. $\bigcirc$ 송이(Ф1~12mm) : 합섬A와 합섬B 급액천에서는 17.0~53.8ml가 유입되었으나 합섬C와 실에서는 시간당 평균 4.0~21.2ml가 흘러나와 배지의 공극이 일정하지 않아 시간당 통과하는 양이 일정하지 않았다고 생각되었다. $\bigcirc$ 펄라이트 : 합섬A(비스코스+레이온)급액천의 유입은 소(1$\times$60cm)에서 21.8ml, 중(2$\times$60cm) 33.5ml, 대(3$\times$60cm) 43.4ml가 통과되었고 합섬(폴리에스텔)에서는 19.0~30.7ml로서 급액천의 규격에 따라 통과되는 차이가 있었다. 배지가 규격화되어 있어 급액천의 규격별로 일정하게 유입되었으며 급액천의 재질이 유입에 영향을 미친 것으로 사료되었다. (2) 급액관과 베드상과의 높이에 따른 유출양 : 급액과 베드상과의 낙차가 클수록 유출이 증가함을 알수 있었으나 합섬C(인견)실험구에서는 낙차가 유출에 영향을 미치지 않았다. (4) 급액된 양액의 EC 및 pH조사 : 급액된 양액의 EC 및 pH에 전혀 변화가 없어 재배 적응에 문제가 없을것으로 사료되었다.

• Journal of Bio-Environment Control
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• 제20권4호
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• pp.258-262
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• 2011

This study was carried out to investigate the effect the water content in substrates as according to growth stage on the growth and yield of paprika in summer hydroponics. Treatments of I, II and III were composed of 55-65-60, 50-60-55 and 45-55-50 % in water contents of growth stages, respectively. Time domain reflectometry (TDR) sensors were used in a drip irrigation system. The early growth of paprika was increased by high medium water content of treatment I. Mean fruit weight was not affected by medium water content, but the fruit number per plant and yield were increased at high medium water content of treatment I. The yield of treatment I was higher than that of treatment II and III. The incidence of brown stem fruit, blossom end rot and sunburn was decreased with increasing water content of medium. Occurred in the low water content of medium. The nitrogen (N) was higher content in brown stem fruit than normal stem, but mineral contents such as potassium (K), calcium (Ca), magnesium (Mg) etc. were not affected.

• Journal of Korean Society of Forest Science
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• 제59권1호
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• pp.51-56
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• 1983

Embryo tissue from Pinus koraiensis was established in culture on G.D. and W.S. basal medium supplemented with the auxin (NAA, 2,4-D, IBA) and kinetin. The various combinations of growth regulators were studied in order to determine the specific requirements of the callus tissue in vitro. The inorganic nutrient combination of G.D. medium was found to be better than that of W,S. medium. G,D. basal medium supplemented with 0.1 ppm NAA and 0.1 ppm kinetin was the most successful nutrient combination for the growth of callus induced from the embryo of P. koraiensis.

• Korean Journal of Microbiology
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• 제36권1호
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• pp.74-78
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• 2000

Vibrio, Photobacterium, Alteromonas and Xenorhabdus species are capable of emitting light, called bioluminescence. They exist in marine, freshwater and terrestrial environments. Bacterial bioluminescent reaction is that reduced riboflavin phosphates and a long-chain aldehyde are oxidized in the presence of molecular oxygen and enzyme luciferase. This experiment aims to develop the proper culture media and to optimize the storage condition for the recovery of bioluminescent activity in Photobacterium phosphoreum. The Luria broth (LB) medium was modified for cultivation of Photobacterium phophoreum, called as modified LB(mLB) medium. The mLB medium is LB fortified with 3% glycerol and 1.5% NaCl. In mLB medium. bacterial growth and bioluminescent activity are 25% higher than those in a Nutrient broth medium. When the cell stocks were stored at $-20^{\circ}C$, $-70^{\circ}C$ and LN2 for 3 months, cell growth and bioluminescent activity of culture after stored at $-20^{\circ}C$ were better than those of other treatments. The highest bioluminescent activity obtained at the late exponential phase in all treatments. When the cell stock was freeze-dried with 5% adonitol as a cryoprotectant, the recovery of cell was better than those of control and freeze-dried cell stock without addition of cryoprotectant.