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Morphological Observation of Somatic Embryogenesis in Leaf Explant Cultures of Bupleurum falcatum L. (시호(Bupleurum falcatum L) 잎절편으로부터 형성된 체세포배 발생의 형태학적 관찰)

  • 조덕이;소웅영
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.5
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    • pp.291-298
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    • 1995
  • This study describes plant regeneration from leaf explant of Bupleurum falcatum through somatic embryogenesis, and the effect of 2,4-dichlorophenoxyacetic acid on somatic embryo abnormalities. The relationship between the cotyledon number of somatic embryo and its germinability is also described. Embryogenic calli were selected from calli formed on explants cultured on MS solid basal medium supplemented with 1 mg/L 2,4-D. Cotyledonary abnormalities were observed in somatic embryos which were developed from calli cultured on MS medium with 1 mg/L 2,4-D for 6-week and then subcultured on 2,4-D free MS medium for 3 weeks. The frequency of abnormalities was as follows: 7% of somatic embryos had one cotyledon, 65% of them had two cotyledons, 25% three cotyledons, 5% four cotyledons, 2% five cotyledons, and 3% trumpet-like cotyledons. The two cotyledon somatic embryos were germinated at a frequency of 80%. However the germination frequency of one cotyledon embryo and multicotyledonary embryo was lower than that of the two cotyledon somatic embryo. All of trumper-like somatic embryos did not germinate. Histological observations of multicotyledon embryo showed circular procambium in the root but pocambial strands in the cotyledonary node or upper hypocotyl. The number of the strands was equal to the cotyledon number.

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Impact of Sleep Time on Psychic Symptoms, Suicidal Ideation and Suicide Attempt in Korean Adults : Focus on Gender Difference (우리나라 성인에서 수면시간이 정신증상, 자살생각 및 자살시도에 미치는 영향: 성별차이를 중심으로)

  • Hong, Yeong-Kyung;Lee, Moo-Sik
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.5
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    • pp.372-383
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    • 2020
  • This study analyzed the influence of Korean adults sleep time on psychological symptoms, suicidal ideation and attempts considering gender differences. The data was collected from the community health survey in 2017 with 228,381 people. When categorizing them into male and female, logistic regression analysis was conducted and the main analysis results are as follows. First, there are more women with shorter or longer sleep time than men. Second, in case of shorter sleep time (5 hours and less), stress, depression, and the suicidal ideation rates were high while suicidal attempts were higher in case of sleep greater than 9 hours and more. Third, in case of men, based on 6~8 hours of sleep time, people with shorter sleep time showed higher stress (1.84 times), depression (1.98), suicidal ideation (1.88), and attempts (2.38). People with 9 hours and more sleep showed greater depression (1.79), suicidal ideation (1.96) and attempts (2.39). Fourth, in the case of women, people with shorter sleep time showed greater stress (2.09), depression (1.94), suicidal ideation (1.96), and attempts (2.90). People with longer sleep showed greater depression (1.39), suicidal ideation (1.45) and attempts (1.71). Fifth, people's stress with greater sleep for both genders was higher than that of people with normal sleep. but there was no significant difference. Further prospective studies are needed on specific sleep dysfunctions effects on suicidal ideation and attempts.

The effect of chronic diseases and oral health behavior of economic workers on oral diseases (경제활동자들의 만성질환과 구강건강행태가 구강질환에 미치는 영향)

  • Hong, Min-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.4
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    • pp.245-253
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    • 2018
  • This study investigated 2,088 economically active workers aged 20 to 65 years selected from the sixth Korea National Health and Nutrition Examination Survey to evaluate the relationship between chronic diseases and oral health behaviors among economic workers and examine the risk factors for oral diseases. The results of the study were as follows. Dental caries were related to subjective oral health status, toothache experience, orthodontic treatment experience, chewing problems, speaking problems, oral examination, and dental clinic use. Periodontal disease was associated with oral health status, toothache experience, orthodontic experience, and chewing and speaking problems. The risk factors for chronic diseases and oral health behavior were as follows. Hypertension, 1.37 times; obesity, 1.48 times; diabetes, 1.5 times; low HDL-cholesterol, 1.31 times; hepatitis A antibody, 1.53 times higher. Health status was found to be 1.70 times higher in dental caries and 2.10 times higher in periodontal disease. The tooth experience was 1.30 times higher in dental caries and 1.35 times higher in periodontal disease. Problems chewing were 1.76 times for dental caries and 1.78 times for periodontal disease. The possibility of dental caries and periodontal disease was 1.44 times higher. Smokers were 1.61 times more likely to have dental caries and 1.63 times more likely to have periodontal disease. These results suggest that periodontal diseases increase the risk of chronic diseases and oral health behavior increases the risk of dental caries and periodontal disease. Although dental caries seem to have a lower risk of becoming a chronic disease if only oral health care is good, it is considered that both chronic disease and oral health care are important for periodontal disease.

Studies on Proper Medium for Somatic Embryogenesis in Suspension Culture of Rehmania glutinosa and Encapsulation of Somatic Embryos (지황의 현탁배양에서 체세포배 형성에 관여하는 요인분석과 체세포배의 Encapsulation)

  • Park, Ju-Hyun;Park, Sang-Un;Chae, Young-Am
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.2
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    • pp.100-106
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    • 1995
  • This study was conducted to find the factors affecting somatic embryogenesis in suspension culture of Rehmania glutinosa and investigate the possibility of artificial seed production by encapsulation of somatic embryos. Linsmeier-Skoog medium was appeared as proper for somatic embryogenesis. Sucrose with $3{\sim}5%$ as carbon sources was good for somatic embryogenesis, and both ammonium and nitrate nitrogen were necesary for normal somatic embryo production. BA with NAA or kinetin with NAA were better than the use of cytokinin alone for both somatic embryogenesis and numbers of somatic embryos. $AgNO_3$ as protectant for vitrification of seedlings in vitro culture had no harmful effect on somatic embryos. Sphericity of encapsulated seeds was good at 3% gel of sodium alginate but germination was better at 2.5% sodium alginate level. Artificial seeds were germinated and developed normal shoots and roots under in vitro condition.

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Somatic Embryogenesis in Immature Zygotic Embryo Cultures of Korean Soybean (Glycine max L.) Culitivars and Effect of 2,4-Dichlorophenoxyacetic Acid on Somatic Embryo Morphology (한국 품종 대두(Glycine max L) 미숙배로부터 체세포배발생과 배지의 2,4-Dichlorophenoxyacetic Acid 농도가 체세포배의 형태에 미치는 영향)

  • Pil S Choi;Yoong Y. SOH;Duck Y.Choi;ang R. LIU
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.1
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    • pp.7-14
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    • 1994
  • Immature zygotic embryos of five Korean soybean cultivars cultured on Murashige and Skoog's (MS) medium supplemented with various concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) produced somatic embryos without forming an intervening callus. The highest frequency (up to 90%) of somatic embryo formation was obtained when zygotic embryos were cultlued on medium containing 1 to 2 mga 2, 0-D in four cultivars. However the frequency was highly variable to the cultivars. Transversely sliced primary somatic embryo halves were also capable of forming secondary embryos at frequencies of up to 70% when cultured on medium containing 0.1 to 1 mg/L 2,4-D. Somatic embryos formed on zygotic embryos cultured on medium containing 0.1 to 0.2 mg/L 2,4-D had two cotyledons more frequently than one horn-type cotyledon and those on medium containing 0.5 to 4mg/L 2,4-LD had a horm-type cotyledon at a prominently higher freequency. However somatic embryos on medium containing 10mg/L or higher concentrations of 2,4-D were usually shunted at the globular stage even after transfer to medium containing lower concentrations of 2,4-D or other growth regulators. non somatic embryos with one or two cotyledons or a hem-type cotyledon were transferred to medium containing $GA_3$, those with two cotyledons converted to plantlets at a higher frequency (25%) than the others.

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Correlation between oral frailty and health-related quality of life (HINT-8) among older adults in Korea (한국 노인의 구강노쇠와 건강관련 삶의 질(HINT-8)의 관련성)

  • In-Ja Kim
    • Journal of Korean society of Dental Hygiene
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    • v.24 no.2
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    • pp.109-119
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    • 2024
  • Objectives: This study aimed to confirm the correlation between oral frailty and health-related quality of life (HINT-8) among older adults in Korea. Methods: The data of 1,318 individuals aged ≥65 years who participated in the eighth Korean National Health and Nutrition Examination Survey (2019) were analyzed using complex sample statistical analysis. Results: Chewing discomfort was found to decrease the HINT-8 scores by 1.246, 1.324, and 1.089 times in the physical, social, and mental domains, respectively. Speech discomfort was found to decrease the HINT-8 scores by 1.275, 1.449, and 1.175 times in the physical, social, and mental domains, respectively. The HINT-8 scores of participants with ≤19 natural teeth were lower in the physical and social domains. Similarly, the HINT-8 scores of participants with brushing frequency of ≤2 were lower in the positive health domain. Non-use of oral hygiene products led to a reduction in the HINT-8 score in the social health domain. Conclusions: Oral frailty in older adults reduces the health-related quality of life. Thus, it is necessary to formulate policies to manage oral frailty in this population and develop specialized programs for the management of oral frailty.

Mass Production of Acanthopanax pedunculus through embryogenic cell culture and of madicinal row materials through bioreactor culture (참오갈피(Acanthopanax pedunculus)의 배형성 세포배양을 통한 식물체 대량번식 및 bio-reactor를 통한 약용원료 생산)

  • 정수진;윤의수;최용의
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2002.11b
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    • pp.29-29
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    • 2002
  • 참오갈피(Acanthopanax pedunculus)는 국내 고유한 자생 종이며 현재 몇 그루 만이 존재하는 것으로 추정된다. 이 수종은 대체적으로 다른 오갈피 수종에 비해 잎 및 열매는 물론 식물 전체 부위가 다소 커서 경제성이 높다고 판단된다. 본 실험에서는 참오갈피의 세포배양 기술을 이용한 묘목 및 약용원료의 산업적 대량 생산 기술을 개발하고자 수행 하였다. 참오갈피 접합자배를 적출하여 무균적으로 배양한 다음 자엽단계로 성숙한 이들 접합자배를 1 mg/1 2,4-D가 첨가된 MS 배지에 배양하여 배형성 캘러스를 유도하였다. 배형성 세포의 유도율은 약 72%에 달했다. 유도된 배형성 캘러스는 2,4-D가 첨가된 같은 고체 및 액체 배지에서 계대배양하여 배형성 캘러스 및 세포를 유지하였다. 배형성 캘러스를 2,4-D가 첨가되지 않은 배지에 옮겨주면 이들 세포로부터 체세포배가 발생되었다. 약 2개월의 기간을 거쳐 자엽단계로 성숙된 체세포 배는 발아되지 않기 때문에 5 mg/1 GA$_3$가 첨가된 1/2 MS 배지에 옮겨 발아 및 식물체로 재생 시켰다. 약 7 cm 크기로 재생된 식물체를 인공토양에 옮겨 1달간 순화시키고 난 후 토양에 옮겼을 경우 87%가 생존하였다. 기내에서 유도된 참오갈피 식물체는 특별한 처리를 하여주지 않아도 뿌리로부터 배발생 세포를 형성 할 수 있었다. 따라서 이들 식물체로부터 배발생세포 유도는 세포주의 보존 및 증식에 효과적으로 이용될 수 있었다. 한편 참오갈피 세포는 5-10 리터 바이오리엑터 배양을 통하여 배형성세포, 자엽단계의 배, 유식물의 대량생산의 가능성을 확인하였다.

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Effects of Cell Size, Density, Conditioned Media and pH on Carrot (Daucus carota L.) Cell Embryogenesis (당근(Daucus carota L.) 세포 배양시 세포의 크기, 밀도, Conditioned 배지 및 pH가 배발생에 미치는 영향)

  • 백기엽;이철희;황주광
    • Journal of Plant Biology
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    • v.28 no.2
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    • pp.141-148
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    • 1985
  • The effects of sizes and densities of cells cultured, conditioned medium, and media pH on the somatic embryogenesis of carrot (Daucus carota L.) were examined. A large number of globular embryoids was formed after 4 days in cell culture, and later globular embryoids developed into heart and torpedo shape. High cell density resulted in higher number and better growth of embryos, especially on conditioned medium than Murashige-Skoog medium. The fresh weight and number of embryoids formed increased with the decrease in cell size. The significant reduction in fresh weight and number of embryoids was obtained when culturing cells with diameter of over 90 ${\mu}{\textrm}{m}$. Dry weight and number of embryoids were markedly reduced with medium pH of 4 or 7, but promoted with pH 6.0.

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Aromatic Compounds in Flower of Chinese Yam (Dioscorea batatas Decne) (마(Dioscorea batatas Decne)꽃의 방향성분 조성)

  • 김상국;강동균;이상철;민기군;이승필;정상환
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.748-751
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    • 1997
  • The study was carried out to identify compositions and recovery yield of aromatic compounds of flower in Chinese yam (Dioscorea batatas Decne). Total 26 volatile aromatic compounds were identified and peak area percentage of aliphatic alcohol in Dan-ma was higher than in Jang-ma. Major volatile aromatic compounds analyzed by GC/MS were 1,2-butyleneglycol, phenylalcohol, caproic acid, cinnamic alcohol, and palmitic acid. Recovery yield of essential oils of Dan-ma was higher than in Jang-ma as 0.983%. As a result, it was concluded that Chinese yam was worthy of cultivating as perfume and medicinal crops.

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Deep Learning based x4 and x8 Super-Resolution for Cultural Property Images (딥러닝 기반 문화재 영상에 대한 4 배 및 8 배 초해상화)

  • Son, Chaeyeon;Kim, Soo Ye;Kim, Juyoung;Kim, Munchurl
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2020.11a
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    • pp.118-122
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    • 2020
  • 문화재 영상 데이터는 방대한 양으로 인해 고해상도로 모두 저장이 어렵거나 시간이 지나 상대적으로 화질이 낮은 영상들이 다수 존재하기에 초해상화가 필요한 상황이 많다. 따라서 본 논문에서 처음으로 문화재 영상에 특화된 4 배 및 8 배 딥러닝 기반 초해상화 방식을 제안한다. 문화재 영상 데이터는 배경이 단조롭고 물체가 영상 중간에 위치한다는 특징이 있어 이를 고려해 중간 부분에서만 패치를 추출하는 방식을 적용하여 의미 있는 패치로 학습이 되도록 한다. 또 자연 영상 데이터 셋인 DIV2K 를 사용해 학습하는 방식과 직접 구성한 문화재 데이터 셋을 이용해 학습하는 방식, 그 둘을 적절히 함께 사용하여 학습하는 전이 학습 방법까지 세 가지로 학습하여 초해상화의 성능을 향상시키는 방법을 제안한다. 그 결과, 쌍삼차 보간법(Bicubic interpolation)보다 4 배 초해상화에서는 약 1.25dB, 8 배 초해상화에서는 약 1.26dB 의 성능 개선을 확인하였으며, 단순 DIV2K 로 학습한 방식보다는 4 배에서는 0.06dB, 8 배에서는 0.17dB 의 성능 개선을 확인하였다.

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