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Evaluation of the LG AdvansureTM Malaria P.f./P.v. real-time QPCR for the Diagnosis of Malaria (LG AdvansureTM Malaria P.f./P.v. real-time QPCR의 말라리아 진단 유용성)

  • Lee, Hye Jin;Kim, Ha Nui;Yoo, Byong Joon;Kim, Jang Su;Kim, Myong Han;Lim, Chae Seung;Lee, Kap No
    • Laboratory Medicine Online
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    • v.1 no.2
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    • pp.100-104
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    • 2011
  • Background: Malaria is a problematic disease in Korea, and microscopic examination of Giemsa-stained blood smear has been used as the gold standard for its diagnosis. However, this technique is time-consuming and has low sensitivity in samples with low numbers of malarial parasites (<20 parasites/μL). Here, we evaluated the performance characteristics of the LG AdvansureTM Malaria P.f./P.v. real-time QPCR (LG life sciences, Korea). Methods: Blood samples from 173 persons who visited Korea University Ansan Hospital were evaluated. QPCR was performed in 73 malaria patients and 100 healthy subjects by using the LG Advansure Malaria P.f./P.v. real-time QPCRR kit, and the results were compared with those of microscopy. The detection limit of this kit was determined by serial dilution of Plasmodium-infected blood with normal blood (blood not infected with Plasmodium). Results: Among the 73 patients that were microscopically confirmed to have malaria (Plasmodium vivax infection, N=70, P. falciparum infection, N=3), 69 patients were diagnosed with P. vivax infection and 3 were diagnosed with P. falciparum infection by LG AdvansureTM Malaria P.f./P.v. realtime QPCR. Both the tests indicated absence of infection in the 100 healthy subjects. The detection limit of LG AdvansureTM Malaria P.f./P.v. real-time QPCR was 0.1 parasite/μL. Conclusions: LG AdvansureTM Malaria P.f./P.v. real-time QPCRis a very sensitive and specific technique and can be used as a confirmatory test for malaria.

Adsorption Characteristics of Oxyanions on Ferrihydrite and Mineral Phase Transformation (페리하이드라이트의 산화음이온 흡착 특성과 광물상 변화)

  • Gyure Kim;Yeongkyoo Kim
    • Economic and Environmental Geology
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    • v.56 no.3
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    • pp.301-310
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    • 2023
  • Ferrihydrite is an iron oxide mineral that is easily found in the natural environment, including acid mine drainage, and has a low crystallinity and high specific surface area, resulting in high reactivity with other ions, and can remove environmentally hazardous substances. However, because ferrihydrite is a metastable mineral, there is a possibility of releasing adsorbed ions by phase transformation to other minerals having low surface area and high crystallinity. In this study, the adsorption characteristics of arsenate, chromate, and selenate on ferrihydrite and the oxyanion removal efficiency of ferrihydrite were studied considering mineral phase transformation. At both pH 4 and 8, the adsorption of oxyanions used in the study were in good agreement with both Langmuir and Freundlich adsorption models except for selenate at pH 8. Due to the difference in surface charge according to pH, at pH 4 a higher amount of ions were adsorbed than at pH 8. The adsorption amount were in the order of arsenate, chromate, and selenate. These different adsorption models and adsorption amounts were due to different adsorption mechanisms for each oxyanions on the surface of ferrihydrite. These adsorption characteristics were closely related to changes in the mineral phase. At pH 4, a phase transformation to goethite or hematite was observed, but only a phase transformation to hematite was observed at pH 8. Among the oxyanion species on ferrihydrite, arsenate showed the highest adsorption capacity and hardly caused phase transformation during the experimental period after adsorption. Contrary to this, chromate and selenate showed faster mineral phase transformation than arsenate, and selenate had the lowest retardation effect among the three oxyanions. Ferrihydrite can effectively remove arsenate due to its high adsorption capacity and low phase transformation rate. However, the removal efficiency for other two oxyanions were low by the low adsorption amount and additional mineral phase transformation. For chromate, the efficient removal is expected only at low concentrations in low pH environments.

Conceptual Model of Establishing Lifestyle (Lifestyle-DEPER [Decision, Execution, Personal Factor, Environment, Resources]) and Lifestyle Intervention Strategies (라이프스타일 형성 모델(Lifestyle-DEPER [Decision, Execution, Personal Factor, Environment, Resources])과 건강을 위한 라이프스타일 중재 전략)

  • Park, Ji-Hyuk;Park, Hae Yean;Hong, Ickpyo;Han, Dae-Sung;Lim, Young-Myoung;Kim, Ah-Ram;Nam, Sanghun;Park, Kang-Hyun;Lim, Seungju;Bae, Suyeong;Jin, Yeonju
    • Therapeutic Science for Rehabilitation
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    • v.12 no.4
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    • pp.9-22
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    • 2023
  • The Lifestyle-DEPER (Decision, Execution, Personal Factors, Environment, Resources) model explains lifestyle formation. Lifestyles are shaped through the decision, execution, and habituation stages. Factors influencing the establishment of a lifestyle are categorized as environmental, resource, and personal. The environment encompasses our surroundings and social, physical, cultural, and virtual environments. Resources refer to what individuals possess, such as health, time, economic, and social resources. Personal factors include competencies, needs, and values. At the lifestyle establishment stage, each of these factors influences a different stage. These collective processes are referred to as events, encompassing both personal and social events. Health-related lifestyle factors include physical activity, nutrition, social relationships, and occupational participation. These are the goals of lifestyle intervention. The intervention strategy based on the Lifestyle-DEPER model, called KEEP (Knowledge, Evaluation, Experience, Plan), is a comprehensive approach to promoting a healthy lifestyle by considering lifestyle formation stages and their influencing factors. This study introduces the Lifestyle-DEPER model and presents a lifestyle intervention strategy (KEEP) to promote health. Further research is required to validate the practicality of the model after applying interventions based on the lifestyle construction model.