• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.320-325
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• 2011

This experiment was conducted to study the effects of germination condition, nursery media and nutrient concentration in plant factory on the growth characteristics of pak-choi and lettuce seedlings. The results showed that temperature (15, 20, $25^{\circ}C$) and light conditions (lighting and no lighting) had no significant effect on germination rate of pak-choi and lettuce, whereas the interaction of growing media (rockwool and urethane sponge) and moisture management before and after sowing had significant effect on germination percentage. In case of no moisture management before and after sowing, germination percentage was much higher in rockwool medium than urethane sponge medium. But germination percentage of urethane sponge was higher than that of rockwool with applying moisture before and after sowing. At the nutrient concentration of EC $1.0dS{\cdot}m^{-1}$ the fresh weight of pak-choi was heavier than in EC 1.5 and $0.5dS{\cdot}m^{-1}$ by 1.8 and 6.0 times, respectively. At the nutrient concentration of EC $1.0dS{\cdot}m^{-1}$ the fresh weight of lettuce was also heavier than in EC 1.5 and $0.5dS{\cdot}m^{-1}$ by 1.7 and 3.5 times, respectively.

• Applied Biological Chemistry
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• v.26 no.1
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• pp.28-34
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• 1983

The change of lactate dehydrogenase(LDH) activity and the possibility of the existence of LDH. isozyme were examined with different parts of soybean sprout. The enzyme activity was little changed in cotyledons throughout the early stagy of germination. However, hypocotyls and roots showed the continuous decline of the enzyme activity since the radicle emerged from seeds. It was found that LDH from hypocotyls. and roots was unstable as compared with LDH from cotyledons, even at low temperature. The enzyme from hypocotyls and roots was not purified with a good yield when the purification procedure developed for LDH from cotyledons. was employed. LDH from hypocotyls and roots has the Rm value of 0.29, and 0.25 from cotyledons. The apparent Km value for LDH from cotyledons was 0.45mM with sodium pyruvate, while crude homogenate of hypocotyls or roots showed biphasic phenomenon with two Km values 0.014 and 0.45mM. The results indicate the possibility that crude homogenate of hypocotyls or roots may contain a different LDH isozyme from the LDH of soybean reported previously.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.2
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• pp.123-128
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• 1986

The characteristics such as characters of seed, fruit and endosperm, development of embryo, dehisced and germination rate of seeds which harvested at 10 days intervals from 20 days to 60 days after pollination were determined to clarify the optimum time for seed production of ginseng. The sizes of length, width and thickness of fruits, seeds and endosperms investigated on 20 days after pollination were not largely different from those that harvested after 60 days. But the weights of fruits and seeds were increased along with the delay of harvesting time. The seeds which harvested from 30 to 60 days showed comparatively high dehisced and germinated rates, especially 40 days in dehisced rates and 50 days in germinated rates. The embryos were rapidly grown in early stage. Lengths of the embryo on 20 days after pollination were approximately grown to 30 ${\mu}{\textrm}{m}$. And after 30 days pollinated, embryos were not only more rapidly grown to 213 ${\mu}{\textrm}{m}$ but also cotyledon premodia were developed in this time and completely formed after 40 days.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.223-229
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• 2014

The objective of this study was to evaluate quality properties including amylose, alkali digestion value (ADV), texture and GABA (Gamma Amino-Butyric Acid) of brown rice and 1~5 mm germinated brown rice of Keunnun and Samkwang culitvars. The protein content of germinated brown rice showed increasing tendency according to shoot length, however, the difference was not statistically significant. The amylose content of all samples ranged from 15.0% to 16.0% in Keunnun and 17.0% to 18.0% in Samkwang, without significant differences. Compared to regular brown rice, germinated brown rice from both cultivars demonstrated reduced gel consistency, which contributed to the improved cooked rice texture. Especially, germinated brown rice had significantly lower setback values, which is correlated with high palatability of cooked rice. However, there was no significant difference in gel consistency between 1 mm and 2~5 mm germinated brown rice. GABA content in Keunnun increased by 3~5 times after germination process. In Samkwang, GABA content increased by 50 times (from $0.005{\mu}g/g$ to $0.243{\sim}0.247{\mu}g/g$) after germination. The hardness of germinated brown rice was significantly lower than that of brown rice. However, there was no significant difference in hardness according to shoot length. Consequently, when processing germinate brown rice, it is recommended to maximize functional ingredients by germination while maintaining shoot length less than 2 mm.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.1
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• pp.31-37
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• 1993

It is important to improve the germination of grass seeds because they are poor germination under water stress. This experiment was conducted to investigate the response of seeds pre-treated individually with Polyethylene glycol 6000 (PEG) and growth regulators(GA$_3$, Kinetin, NAA) on the germination characteristics when two levels of osmotic potential (0, -5 bar) were put to seeds of Orchardgrass. In the untreated seeds, the total germination rate was decreased under water stress, and the mean germination time was delayed. Priming of Orchardgrass seeds using PEG or GA$_3$ resulted in improved germination under water stress(-5 bar), whereas the opposite was true of kinetin or NAA. The response of seeds primed in solutions of either -15 bar PEG or 100 ppm GA$_3$ was particulary marked compared with the untreated seeds. It is suggested that PEG and GA$_3$ may have positive regulatory effects in triggering the system for water stress alleviation. But the inhibitory effect of the water stress was not completely removed by allowing the seeds to pre-treat in solution of PEG or GA$_3$.

• Korean Journal of Plant Resources
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• v.14 no.2
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• pp.108-115
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• 2001

For determining the effect of extracts exudated from ten industrial plant resources on germination and early growth of shoot and root of radish the allelopathic compounds screened. Germination rate and seedling growth of radish differed depending on plant species and extract concentration. Lower levels of 1% extracts exudated from industrial plant resources promoted the rate of germination of radish while higher levels of 10% extract inhibited the rate of germination. Germination rate, shoot growth and root growth of radish in higher concentration of extract also differed depending on the plant species exudated and extract concentraton of industrial plant resources. Higher concentration of extracts from Xanthium strutnarium L. Angellica gigas Nakai, and Solanum nigrum L. were completely inhibited the germination while those from Cichorium intybus L., and Polygonatum officinale Allioni were not inhibited. Extracts exudated from Xanthium strutnarium L, Angellica gigas Nakai, and Solanum nigrum L. showed the strong inhibition of shoot and root growth of radish while those from Cichorium intybus L., and Polygonatum officinale Allioni did not show the bioactivity in shoot and root growth of radish..

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.3
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• pp.268-276
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• 1987

Five soybean varieties were used to measure seed vigor according to the artificially different deterioration. A vigor index derived from the product of percent germination x hypocotyllength was used as the parameter in comparison of other tests for seed vigor. There were the varietal differences in initial vigor. The warm germination test was the best measurement for seed vigor at the advanced stages of seed deterioration. All other vigor measurements, except ATP and GADA measurements, showed highly significant correlations with the vigor index. Hypocotyl length, conductivity index and cold germination measurements for seed vigor were appropriate for predicting seed quality.

• Korean Journal of Medicinal Crop Science
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• v.11 no.5
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• pp.347-351
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• 2003

This experiment was carried out to study the optimal chilling period for breaking the physiological dormancy of dehisced E. senticosus seeds and to investigate the critical seeding date in Kangwondo. Storage at $5^{\circ}C$ for 85 days was most effective in breaking dormancy of dehisced seeds, but didn't germinated synchronously. Only 28.1% of undehisced seeds germinated after 125 days of low temperature storage. For improving seedling survival rate in the field, 50% shading was more effective than 30% shading. Delayed seeding date resulted in decreased seedling fresh weight, dry weight, leaf area and root length, but seeding date has no significant affecte on stem length.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.6
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• pp.847-854
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• 2003

In general cooked rice would be used as a major raw material for making Takju. In this case quality, taste and storage time of Takju were not fully satisfied. Fermentation conditions for Takiu mash were examined by using the germed brown rice in this study. In case of the germed brown rice on 2nd stage mash, alcohol was slowly generated in comparison with the cooked rice. Reducing sugar was slowly produced and the amount of reducing sugar was low. The sugar content was created at a uniform rate. The pH was shown to be higher in mash of the germed brown rice than that of the cooked rice. Acidity change showed a similar inclination to pH change. The degree of yeast growth on the mash of the germed brown rice was revealed to be slightly lower than that of the cooked rice. Temperature of mash was kept to be constant after 3 days from fermentation. Fusel oil produced from the mash of the germed brown rice was less gernerated in comparison with the cooked rice. Amount of amino acid in case of the germed brown rice was indicated to be higher. Takju made with the germed brown rice was shown to be 1.3 times in overall taste, 1.5 times in refreshing as compared with Takju made with the cooked rice. However there are no differences between them in flavor and color of Takju. In overall acceptance Takju made with the germed brown rice was shown to be 1.3 times as compared with Takju made with the cooked rice. In conclusion the germed brown rice was expected to be able to be better in Takju quality.

• Korean Journal of Medicinal Crop Science
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• v.3 no.1
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• pp.50-55
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• 1995

This study was conducted to determine the optimum conditions for induction and different somatic of somatic embryos as well as germination of encapsulated and stored somatic embryos. Somatic embryos was better formed in 1/2X MS medium than full - strength MS medium. 0.1 to 1.0mg/lBA and kinetin promoted shoot differentiation of somatic embryos. Higher concentration tend to inhibit differentiation. IAA affect positively both root and shoot growth. In vitro germination of somatic embryos encapsulated with 2% alginate matrix containing 1/2 MS nutrient medium and $AgNO_3$ 5mg/l was 86%. Storage of somatic embryos was effecive at $5^{\circ}C$ but the germination rate decreased with longer storage period. RAPD analyses with plants regenerated from the somatic embryos showed DNA polymorphism, indicating abolition of primer binding site by point mutation, deletion, or insertion of certain sequences.