• Title/Summary/Keyword: 배란유도

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Effect of Repeated Induction of Superovulation on Ovulation Rates and In Vitro Development of Embryos in Rabbit (토끼에서 반복적인 과배란유도가 배란율과 난자의 체외 발육율에 미치는 영향)

  • 진동일;임경순;이홍미
    • Journal of Embryo Transfer
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    • v.12 no.3
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    • pp.253-258
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    • 1997
  • 토끼에서는 수정란 이식과 같은 기본적인 번식공학적 방법의 효율성이 아직 생쥐와 같은 실험동물에 비해 떨어지고 있어 생물공학적인 기술을 응용하는데 큰 어려움이 있다. 특히 유전자 이식에 의한 형질전환 토끼의 생산과 같은 생물공학적인 기술을 실용화하는데 효율이 높은 수정란이식 기술의 개발이 필수적이라고 할 수 있다. 본 연구에서는 토끼에서 수정란 이식 기술의 첫 단계인 과배란 유도를 효율적으로 이용할 수 있는 방법을 정립하기 위해 반복적인 과배란 유도가 배란율 및 수정란의 질적인 면과 양적인 면에 미치는 영향을 조사하였다. 연구방법으로는 FSH와 HCG를 사용하여 과배란을 유도하였고 2.5 개월의 반복처리간격으로 3번의 반복적인 과배란 처리를 한 후 반복처리에 따른 배란율과 배란된 난자의 형태학적 상태, 배양에 의한 발생 능력 상태 등을 감소하였으며 배란수의 변이도 커지는 경향을 나타내었다(첫번째, 32.6(+-)2.5; 두번째 28.7(+-)3.7; 세번째 20.99(+-)3.8). 제 2극체의 돌출, 전핵의 형성, cummulus cell의 존재등에 의한 회수된 난자의 형태학적 관찰에 의한 방법으로 난자를 분류한 결과 과배란의 반복수가 증가함에 따라 다양한 모양의 난자가 회수되어 배란이 광범위한 시간대에 일어나고 있음을 나타내었다. 또한 과배란의 반복적인 유도에 의해 난소의 혈포수는 증가하였으나 채란된 난자의 채외배양에 의한 발육율에는 차이가 없었다. 그러므로 과배란의 반복적인 유도는 공란토의 난소반응에는 영향을 미쳤으나 난자의 질에는 영향을 미치지 않았음을 나타낸다.

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Effects of Superovulation Induction on Embryo Quantity and Quality in Rat (과배란 방법이 Rat 수정란의 양과 질에 미치는 영향)

  • 진동일;양무희
    • Journal of Embryo Transfer
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    • v.12 no.2
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    • pp.127-132
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    • 1997
  • 본 연구는 rat에서 PMSG도는 FSH 처리에 의한 과배란 유도가 배란율과 수정란의 질에 미치는 영향을 알아보기 위해 호르몬 처리하고 교미시킨 후 4일령에 난관과 자궁을 세척하여 정상 8-세포기 난자와 비정상 난자를 조사하였고 각 처리에서 채란된 난자 중에 정상난자를 골라 체외 배양하여 발육율을 비교 평가하였다. 미성숙 rat에서는 평균19.1개의 수정란이 채취되었으며 성숙rat에서는 14.2개가 채취되었고 미성숙 rat에서는 성숙 rat에 비해 더 많은 비율의 비정상적인 난자가 회수되었다. FSH와 LH-RH에 의한 방법이 PMSG와 HCG에 의한 방법보다 유의성 있게 많은 난자를 배란시켰으며, 비정상란의 빈도도 낮은 것으로 나타났다. 그러나 호르몬 처리에 의한 두 가지 방법은 자연배란에 의한 방법에 비해 훨씬 높은 비정상난자의 배란을 유도하였다(FSH, 20.1%;PMSG, 41.2%;자연배란 13.4%). 또한 FSH처리에 의해 회수된 난자보다 체외 발육율이 높은 것으로 나타났다. 그러므로 rat에서 PMSG와 FSH를 이용하여 과배란을 유도할 수 있으나 배란된 난자의 비정상율은 자연배란에 비해 훨씬 높았고, 과배란 유도시 호르몬의 종류에 따라 체외 배양율에도 영향을 미치는 것으로 나타났다.

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Induction of Ovulation by LHRH-a and Pimozide in the Bullhead, Pseudobagrus fulvidraco (LHRH-a 와 pimozide에 의한 동자개의 배란유도)

  • 박홍양;이종영;이영직;권혁추
    • Journal of Aquaculture
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    • v.11 no.2
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    • pp.151-158
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    • 1998
  • Effects of intraperitoneal administration of LHRH-a (luteinizing hormone releasing hormone analogue), pimozide and HCG (human chorionic gonadortropin) were studied on ovulation in the bullhead, Pseudobagrus. fulvidraco. Ovulatin was induced in 71.4, 80 and 100% by injection of HCG 5,000, 10,000 and 20,000 IU/kg of body weight, respectively. Also, the majority of fish injected with LHRH-a in the presence or absence of pimozide were ovulated. The ovulating rates in fish injected with LHRH-a alone showed 50, 75 and 100% at a dose 200, 300 and $400^{\mu}$g/kg, respectively. Pimozide enhanced the activity of LHRH-a at $300^{\mu}$g/kg plus pimozide at 1 mg/kg. Pimozide alone was ineffective in inducing ovulation of P. fulvidraco. There were no significant differences in gonadosomatic index (GSI) and pseudo-GSI values among the groups treated with HCG, LHRH-a and LHRH-a plus pimozide (P>0.05). The latency time groups treated with HCG was 17~22 hours, and the group injected with LHRH-a alone and/or in combination with pimozide was ovulated within 23~29 hours.

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Pattern of Ovulation in Korean Women with Premature Ovarian Failure (한국인 조기난소부전 여성의 배란양상)

  • Kim, Hye-Min;Kim, Jae-Ryoung;Choi, Eun-Joo;Park, Eun-Joo;Hong, Seo-Yu;Lee, Jin-Yong;Park, Won-Il
    • Clinical and Experimental Reproductive Medicine
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    • v.35 no.3
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    • pp.231-238
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    • 2008
  • Objective: Significant portion of premature ovarian failure patients ovulate intermittently and even can achieve pregnancy. The clinical features and reproductive potentials of Korean women with premature ovarian failure have never been elucidated. The purpose of this study is to assess ovarian follicular growth and ovulatory potential. Methods: Complete medical records of fifteen women with idiopathic premature ovarian failure were reviewed retrospectively. Subjects with more than 6 ultrasonographic scans and 36 months of follow-up period were included. Once the ovarian follicle was found by ultrasonography, the follicular growth was serially monitored. The result of ovulation induction of 6 cycles in 4 patients were also evaluated. Rate of positive ovarian follicle by ultrasound, rate of natural follicular growth and response of ovulation induction were assessed. Results: Among the 15 patients, 6 women (40%) had ovarian follicle in random ultrasonography. But the chance of positive follicle per ultrasonography scan was 7% (8 out of 115 scans). One out of 6 women eventually ovulated during the follow-up scan. In remaining 5 subjects, size of follicles remained unchanged. None of 6 ovulation induction cycles resulted successful ovulation. Conclusion: Incidence of positive ovarian follicle in Korean premature ovarian failure patients is lower than that of Western counterpart. This result, however, could not be directly compared because of the differences of study design. The ovulation rate by medical ovulation induction in this group is 0%, which is worse than the reported success rate of Western studies.

LHRHa 주사에 의한 양식산 은어(Plecoglossus altivelis) 배란의 촉진 및 동시화

  • 손송정
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 1998.07a
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    • pp.30-31
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    • 1998
  • 1. LHRHa를 정상배란 시기의 30일전부터 10일 간격으로 3회 주사한 것과 20일 전부터 2회 주사한 모든 실험구에서 난소의 성숙촉진과 배란의 동시화 효과가 나타났다. 2. 은어 배란의 도시화가 유도하고 발안란의 생조율을 높이기 위한 LHRHa 주사방법은 어체중 100g 당 10 $\mu$ g을 정상배란 30일전부터 3회 혹은 20일전부터 2회 주사하는 것이 바람직하였다. 3. LHRHa 주사에 의해 배란된 알의 발안기까지의 생존율은 LHRHa 주사 농도가 높을수록 낮아지는 경향을 보였다. 4. 혈장 estradiol과 testosterone의 농도는 LHR 주사후 배란기까지 증가하는 경향을 보였다.

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Induced Ovulation by Hormone Treatments in Red Spotted Grouper, Epinephelus akaara (호르몬 처리에 의한 붉바리 Epinephelus akaara의 배란유도)

  • Park, Jong-Youn;Cho, Jae-Kwon;Park, Chung-Kug;Han, Kyeong-Ho;Hong, Chang-Gi
    • Journal of Marine Life Science
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    • v.4 no.2
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    • pp.63-69
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    • 2019
  • This study aims to investigate how ovulation induction of red spotted grouper, Epinephelus akaara varies when treated with different hormonal substances such as ovaprim, pimozide, LHRHa, and HCG. As for LHRHa, we injected it at four different concentrations: 50 ㎍kg-1, 100 ㎍kg-1, 150 ㎍kg-1, and 200 ㎍kg-1. All hormonal substances including LHRHa were injected into the back muscles. Buoyant, embryonic survival, fertilization, and hatching rate were the highest in LHRHa and LHRHa + pimozide group. When LHRHa was injected at different concentrations, buoyancy and fertilization rate were the highest in 100 ㎍kg-1 group. Embryonic survival and hatching rate were the highest in 150 ㎍kg-1. Based on these experimental results, we concluded that LHRHa at the concentration of 100 ㎍kg-1 is the most efficient for the ovulation induction in this fish species.

Effects of Aromatase Inhibitor on Reproductive Hormone Profiles and Ovulation Induction (방향화효소억제제의 생식호르몬 분비와 배란유도에 대한 효과)

  • Kim, Sook-Hyun;Kim, Jeong-Ah;Park, Joon-Cheol;Bae, Jin-Gon;Shin, So-Jin;Kwon, Sang-Hoon;Cho, Chi-Heum;Yoon, Sung-Do;Cha, Soon-Do;Kim, Jong-In;Rhee, Jeong-Ho
    • Clinical and Experimental Reproductive Medicine
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    • v.35 no.2
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    • pp.143-153
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    • 2008
  • Objective: To investigate the effects of aromatase inhibitor on reproductive hormone profiles and evaluate it's ovulation inducing capability in anovulatory infertile women. Methods: We quantified the blood levels of reproductive hormones from 30 healthy normal cycling women in natural cycle (control) and letrozole medicated cycle (study). LH, FSH, estradiol, testosterone, DHEA-S were quantified on third, 11th, 21th day in both cycles, and on 21th day, progesterone was added. Sixth anovulatory infertile women received either letrozole or clomiphene citrate for ovulation induction (n=30 in each groups). We compared the clinical parameters such as ovulation rate, pregnancy rate, the day of LH surge, number of follicles and endometrial thickness, cervical mucus amount, spinnbarkeit, mean diameter of follicles on the day of LH surge. Results: Letrozole had no effect on the LH, FSH, estradiol, DHEA-S secretion but there were significant increase in testosterone level on day 11 and progesterone level on day 21 in letrozole medicated cycle compared than control cycle ($0.40{\pm}0.16$ vs $0.28{\pm}0.11\;ng/ml$, p=0.002, $18.18{\pm}13.07$ vs $8.38{\pm}7.64\;ng/ml$, p=0.001, respectively). In comparison between letrozole and clomiphene groups, there were no significant difference in ovulation rate, pregnancy rate, number of mature follicle, mean diameter of follicles, but showed earlier LH surge, thicker endometrium, more cervical mucus, and higher spinnbarkeit in letrozole group ($12.12{\pm}2.46$ vs $14.52{\pm}3.18$ days, p=0.006, $10.48{\pm}1.23$ vs $8.52{\pm}0.93\;mm$, p=0.000, $2.04{\pm}0.61$ vs $1.57{\pm}0.59$, p=0.012, $6.00{\pm}1.12$ vs $4.95{\pm}1.61\;cm$, p=0.003, respectively). Conclusion: Letrozole may augment folliculogenesis and improve the endometrial condition for implantation in normal cycling women. Ovulation efficacy of letrozole in anovulatory women was comparable to clomiphene citrate and letrozole may be more physiological in ovulation induction.

Effectof of Hormonal Treatment of Induced Maturation and Ovulation in the Spotted Halibut, Verapser variegatus (호르몬 처리에 의한 범가자미, Verasper variegatus 의 성숙과 배란유도)

  • Baek, Hea-Ja;Kim, Yoon;An, Cheul-Min;Cho, Kee-Chae;Myeong, Jeong-In;Lee, Nam-Ju
    • Journal of Aquaculture
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    • v.13 no.1
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    • pp.47-53
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    • 2000
  • Hormone induction in maturation and ovulation was carried out with spotted halibut (Verasper variegatus) which normally does not spawn spontaneously in captivity. Prior to spawning females were treated with human chorionic gonadotropin(HCG 265-678 IU/kg BM) 17 ${\alpha}$-hydroxy 20-${\beta}$-dihydroprogesterone (17 ${\alpha}$ 20${\beta}$ OHP 0.5-1.0${\mu}$g/kg BW) and luteinizing hormone-releasing hormone analogue pellet (LHRHa pellet 63-81 ${\mu}$g/kg BW) In all the trials HCG injection succeeded in inducing ovulation at 265-300 IU/kg BW. HCG at 620 or 678 IU/kg BW was ineffective in inducing a good rate of ovulation. Final maturation and ovulation were not stimulated by treatment of 17 ${\alpha}$ 20${\beta}$OHP and LHRHa pellet. These results suggest that HCG injection (around 300 IU/kg BW) is more effective compared with LHRHa pellet implantation.

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