• Proceedings of the Korean Society of Developmental Biology Conference
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• 2005.10a
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• pp.134-134
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• 2005

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2005.10a
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• pp.134-134
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• 2005

• Journal of Embryo Transfer
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• v.22 no.1
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• pp.47-51
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• 2007

The objective of this study was to improve the reproductive ability of Thoroughbred mares with artificial estrous regulation by hormone treatments and artificial illumination. The results were as follows; Estrous detection in cycling mares which were treated $PGF_2{\alpha}$ or altrenogest administration was all 100%, and pregnancy rates were 95.2% and 71.4%, respectively. Estrous detection was 100% within March when altrenogest was administered alone or together with estradiol for non-pregnant mares in the previous year. Interval to estrous detection after altrenogest administration was 4.3 days in single administration of altrenogest and 3.7 days in combined administrations of altrenogest and estradiol, respectively Interval to ovulation after estrous detection were 2.7 and 2.5days, respectively. Pregnancy rate following single altrenogest administration was 80.0%. Estrous detection and pregnancy rates by artificial illumination in non-pregnant mares were 92.9% and 46.9%, respectively. These results show that estrous synchronization of mares during breeding season will be able to improve the pregnancy rate, and altrenogest administration in non-pregnant mares in the previous year will be able to induce early reproduction and improve pregnancy rate In racing horses.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.284-284
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• 2004

본 연구는 Elk 사슴에서 Magic Doctor(한경게놈텍)를 이용하여 발정동기화 인공수정 후 재발정 행동의 발현양상을 관찰하기 위해 실시하였다. 미경산록 7두와 경산록 13두를 공시하였고, 발종동기화 및 인공수정은 번식계절인 9월에 progesterone이 3 g 함유된 controlled intravaginal drug-releasing device(CIDR)를 14일간 질내에 삽입하고 제거한 후에 pregnant mare serum gonadotropin(PMSG) 250IU틀 근육 주사하여 발정동기화와 배란을 유도하였다. 인공수정은 CIDR제거 후 60∼62시간째에 실시하였고 인공수정 후 비임신 개체의 재발정 발현 양상을 관찰하였다. (중략)

• Journal of Embryo Transfer
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• v.22 no.1
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• pp.15-19
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• 2007

The objective of this study was to investigate the relationship between body condition score (BCS), blood urea nitrogen (BUN) and estrous expression for the purpose of improving reproductive performance. In total, 37 ovulations and 28 estrous detection were observed among 51 Holstein-Friesian dairy cows. The estrous inducement rate and estrous expression rate were significantly lower for cows with BCS below 2.0 than for cows with BCS above 2.0. There was 0% of rate of standing heat in cows with BCS below 2.0 whereas the rate of standing heat was markedly increased in cows with BCS above 2.0 (46.7% and 64.7% for BCS $2.0{\sim}2.49$ and BCS $2.5{\sim}3.0$ cows, respectively). The estrous expression rate was significantly lower for cows with BUN below 10mg/dl than for cows with BUN above 10mg/dl. There was no significant difference among duration time of estrus, estrous behavior patterns and BUN concentration. The rate of estrous expression and concentration of BUN was not significantly different between primiparous and multiparous cows. This result shows that the level of BCS and BUN affect the estrous expression. Considering the situation that estrous expression is decreased in recent years, effective nutritional management should be accompanied to improve reproductive performance.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.93-93
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• 2003

소의 수정란이식에 있어서 성공적인 임신을 위해서는 수란우의 적절한 영양상태, 양질의 수정란, 이식 시술자의 기술력과 수란우와 수정란의 적절한 동기화가 필수 요인이라 사료된다. 특히, 적절한 동기화를 위해서 발정관찰은 필수적이지만 번거롭고 장시간동안 관찰을 해야 하는 단점이 있다. 따라서 본 연구에서는 이러한 단점을 보완할 수 있도록 발정동기화 처리방법을 비교, 검토하여 수정란이식에 있어서 효과적인 발정동기화 방법을 모색하고자 실시하였다. 발정동기화 방법은 Fig 1과 같이 CIDR처리방법(A군)과 GnRH 처리방법(B군)을 사용하였으며, 황체의 등급은 직장검사와 초음파 진단기를 이용하여 직경이 2cm 이상, 1~2cm와 1cm 미만의 황체를 각각 1등급, 2등급과 3등급으로 분류하였다. 또한 본 실험에 공시된 수정란은 체외 생산된 한우 배반포기의 수정란을 사용하였으며, 1등급의 황체를 가진 수란우만을 선별하여 비외과적인 방법으로 황체가 존재하는 자궁각심부에 이식하였다. 임신진단은 이식 후 45~60일에 직장검사와 초음파진단을 이용하여 실시하였다. 발정동기화처리결과는 Table 1에서 보는 바와 같이 A군과 B군에서 발정발현율이 각각 100%와 96%로 나타났으며, 이식하기에 적합한 1등급 황체의 출현율이 65.4%와 56%로 A군에서 다소 높게 나타났다. 발정동기화 처리방법에 따른 수정란 이식 후 수태율은 A군에서 신선란과 동결란일 때 각각 66.7%와 60%로 나타나 B군의 22.2%와 0%의 결과보다 유의적으로 높은 결과를 나타내었다. 따라서 본 연구의 결과로 미루어 볼 때 수정란 이식을 위한 발정동기화 방법은 CIDR 처리방법을 적용하는 것이 GnRH 처리방법 보다 효율적이라 사료된다.다. 특히 기능황체에서의 특이적 발현 spot을 확인할 수 있었다. 이러한 결과들로부터 황체의 progesterone분비기능의 역할을 수행하기 위한 단백질들이 전, 중기에 발현된다는 것을 알 수 있고 퇴행황체에서는 발현이 안되고 있는 것을 알 수 있다. 이러한 결과를 토대로 다른 양상을 띤 spot을 분리하여 어떤 단백질인지를 분석하여 각각의 황체단백질의 특성을 규명할 수 있을 것으로 사료된다.적율(HCT)을 이루고 있음을 알 수 있었다. 적혈구 평균용적(Mean Corpuscular Volume ; MCV), 평균적혈구혈색소량(Mean Corpuscular Hemoglobin ; MCH), 평균적혈구혈색소농도(Mean Corpuscular Hemoglobin Concentration ; MCHC) 그리고 혈소판(Platelets) 분석결과 형질전환돼지가 일반돼지보다 약간 높은 수치를 나타냈으며 변화양상 또한 유사한 결과를 나타내었다. 이와 같은 시험분석결과를 토대로 사람 조혈촉진유전자(hEPO)가 형질전환된 돼지는 유즙으로 발현할 수 있도록 형질전환 되었음에도 불구하고 헤모글로빈 및 적혈구가 증가함으로서 형질전환돼지 개체의 혈장으로도 사람 조혈촉진인자가 분비하고 있음을 간접적으로 알 수 있었다. 정상돼지 보다 형질전환돼지가 약 30% 높은HCT 수준을 보였으며 이러한 현상은 사람에서는 적혈구증다증(erythrocytosis)으로 분류되고 있다. 이에 대한 고찰은 형질전환돼지 자체의 생리적 문제점(side effects)에 대한 해결과 더불어 기존의 인간질병에 대한 모델동물로서의 이용 가능성을 제시할 수 있는 것으로 사료된다.mu\textrm{m}$ 300BG는 56.32$\mu\tex

• Journal of Embryo Transfer
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• v.21 no.3
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• pp.177-182
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• 2006

This study was conducted to investigate the effects of melengesterol acetate (MGA) and $PGF_{2{\alpha}}$ administrations on serum progesterone level, synchrony of estrus and conception rates in Han-woo. Firstly, ten heifers and one freematin were fed 0.5 mg MGA/day for 14 days in a grain carrier, and after 19 days of MGA feeding, a single injection of 25 mg $PGF_{2{\alpha}}$ were treated. Blood samples were collected to evaluate serum progesterone concentrations from the start of feeding of MGA until the end of feeding and subsequent estrous detection and artificial insemination (AI) at 3 days intervals, and on days of $PGF_{2{\alpha}}$ injection, estrous detection, AI, and 15th and 60th days after AI. The level of progesterone in the blood began to increase from 7 days after MGA feeding, and 9 days after feeding it became 5.4 ng/ml and maintained that level thereafter. On the 33th day when the $PGF_{2{\alpha}}$ was injected, it reached the peak level of 7.6 ng/ml. However, 2-3 days after $PGF_{2{\alpha}}$ injection, it dropped to 1.4 ng/ml drastically (p<0.05). Secondly, one hundred and ninety four Hanwoo heifers or cows were divided into two groups to compare estrous induction and conception rates: the one treated with MGA and $PGF_{2{\alpha}}$, (n=104) and the other with $PGF_{2{\alpha}}$ treatment (two injections at 11 days interval, n=90). The heifers or cows treated with MGA and $PGF_{2{\alpha}}$ were identical to those used as above. The percentages of heifers or cows showed estrus were higher in the $MGA+PGF_{2{\alpha}}$ treatment (91.3%) than in the $PGF_{2{\alpha}}$ treatment (72.2%, p<0.05). Conception rates were also higher in the $MGA+PGF_{2{\alpha}}$ treatment (94.2%) than in the $PGF_{2{\alpha}}$ treatment (88.9%, p<0.05). The results of this experiment indicate that estrus synchronization using $MGA+PGF_{2{\alpha}}$ is more effective than that using $PGF_{2{\alpha}}$ (two injections) in Hanwoo.

• Korean Journal of Animal Reproduction
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• v.26 no.1
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• pp.69-72
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• 2002

Two trials were conducted in a commercial dairy on heifer synchronization with PGF$_2$$\alpha$. Animals showing estrous following the first injection were bred and animals not showing estrus were given the second injection 10 days later. In the first trial, the injection routes were rump and rump. In the second trial, the injection routes were rump and shoulder. Estrous detection were performed from 24 h after injection. Animals were bred by the same technician. In the first trial, the response rate for the first injection was 51.4% and the subsequent pregnancy rate of these animals was 60.0%. The response rate in the second injection was 57.1% and the pregnancy rate was 50.0%. In the second trial, the response rate in the first injection on the rump was 48.7% and the subsequent pregnancy rate was 70.6%. The second injection was given on the shoulder and the response rate was 60.0% and the subsequent pregnancy rate was 25.0%. The data suggest that the route of PGF$_2$$\alpha$ administration was critical to achieve success in estrous synchronization and pregnancy rates.

• Korean Journal of Animal Reproduction
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• v.19 no.2
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• pp.147-152
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• 1995

The purpose of this study was to examine the estrus synchronization and superovulation of pigs with hormone treatments. Three different kinds of procedures for synchronization and superovulation were used as follow: I) gilts in natural estrus behavior (control): 2) gilts synchronized with 20mg altrenogest for 9 days regardless of the estrus cycle; 3) gilts received PG600 (400IU PMSG + 200 IU hCG) in 15 day of the estrus cycle; and then gilts administrated with PMSG (1,500 IU) and hCG (750 IU) after altrenogest and PG600 treatment for superovulation. Estrus was checked daily with a boar, in estrus synchronization, the intervals from hormone treatment to estrus were different between PG600 (43/47) and altrenogest (13/53) within 6 days. The percentage of animals displaying a estrus response were not different by hormone treatments. The average number of corpora lutea (C.L) and ovulated embryos were similar between PG600 25.4${\pm}$13.1, 19.0$\pm$12.8 and altrenogest 25.5${\pm}$0.7, 15.0${\pm}$4.2, respectively, but was increased (P<0.05) by hormone treatment compared to that 12.9${\pm}$1.8, 12.7${\pm}$3.9 in the control. The number of normal embryos after ovulation was higher in the control than hormone treatment. Therefore, these results suggest that altrenogest and PG600 treatment could be a valuable for cut down the labour and cost by synchronization.

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.29-29
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• 2002

본 연구는 이유한 경산돈의 발정동기화방법이 번식성적에 미치는 영향을 조사하기 위하여 대조구(자연 발정), Tl(Regumate＋PMSG＋hCG), T2(PMSG＋hCG) 및 T3(PG600) 처리구로 발정을 유기한 후 발정발현 상태에 따라 수정적기라고 판단되는 시기에 12시간 간격으로 2회 인공수정 하여 분만율 및 산자수를 조사하였다. 본 실험에 사용된 액상정액은 축산 기술연구소 돼지인공수정센타의 종모돈 5두 정액을 혼합하여 이용하였으며, BTS (Beltsville thawing solution) 보존액으로 l회 주입 정자농도를 3.0×10/sup 9/이 되도록 조절하였다. (중략)