• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.31 no.2
• /
• pp.220-225
• /
• 1986

This study was conducted to know the effects of temperature and gibberellic acid on the dormancy block-ing of ginseng seeds at different embryo growth stage. Optimum temperature for embryo growth appeared to be I soc at the beginning stage of post ripening (up to dehiscent time), 1$0^{\circ}C$ at middle stage (for 30 days after dehiscence) and 5$^{\circ}C$ at last stage (between 30 and 92 days after dehiscence). And optimum temperature for dehiscence is about 17$^{\circ}C$, also the optimum temperature for dehiscence is higher than that of embryo growth. Germination of ginseng seed with full grown embryo was accelerated at high temperature (25-3$0^{\circ}C$). Germina-tion percentage was 80% at 105th date after dehiscence under 5$^{\circ}C$, 28% at 147th date under 1$0^{\circ}C$, but no germination under the over 15$^{\circ}C$. Gibberellic acid increased the dehiscent rate, whereas the gibberellic acid treatment may not be substituted for effect of low temperature on the germination. Low temperature may be reguired to finish the embryo growth in thickness.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2022.09a
• /
• pp.67-67
• /
• 2022

먹넌출은 국내에서 안면도에서만 자생하는 희귀식물로, 항산화, 미백, 항암 효능이 밝혀져 유용식물자원으로의 활용이 기대되나 IUCN(세계자연보전연맹) 기준 취약종(VU)으로 분류되어 생명자원의 현지 내, 현지외 보존 필요성이 크다. 따라서 본 연구는 먹넌출의 종자를 이용한 보존과 증식을 위해 기초자료를 확보하고자 하였다. 이를 위해 열매의 성숙도에 따른 형태적 특성과 활력 차이를 비교하고, 종자의 발아 조건 구명을 위해 휴면 종류와 발아 적온을 조사하였다. 먼저 열매 성숙도에 따른 형태적 특성과 활력 조사 결과, 열매가 부분적으로 성숙한 적색 단계는 완전히 성숙한 흑색 단계와 비교하여 종자 무게와 배길이에서 차이가 거의 나타나지 않았고 충실도가 80%, 충실 종자의 활력이 100%로 나타났다. 따라서 종자 채취 시 열매가 흑색으로 성숙하여 떨어지기 전인 적색 단계에서 채취하여도 종자를 이용한 증식에 문제가 없을 것으로 보인다. 또한 먹넌출 종자는 딱딱한 과피로 덮여있지만 수분 흡수를 가능하게 하는 조직을 가지고 있어 물리적 휴면에 해당하지 않았으나 생리적 휴면을 가지고 있었으며, 휴면 타파에는 GA3 1000ppm 처리가 가장 효과적이었다. 또한 발아 온도는 10℃ 12시간, 20℃ 12시간인 변온 조건에서 가장 높은 발아율을 보였다. 본 결과를 먹넌출 종자 수집과 증식에 참고 자료로 활용할 수 있을 것으로 기대한다.

• Journal of Life Science
• /
• v.12 no.6
• /
• pp.721-730
• /
• 2002

This study was carried out to select new pelleting binder and material for Welsh onion seeds. The optimum treatments of the various plant growth regulators to improve seed germination of the Welsh onion was also estimated. There were no significant effects of growth regulators on the germination percentage, but germination was faster according to the number of days to 50% of the final germination ($T_{50}$) and the mean number of days to germination (MDG) than those of the control. Germinability was increased when the seeds were soaked in gibberellic acid ($GA_3$) solution for 24hrs, even though there was no synergy effect on the germinability when $GA_3$ was mixed with 6-benzylaminopurine (BAP). The optimum treatment for improving germination of Welsh onion was observed when the tested seeds was soaked in 500 $\mu$M of $GA_3$ at $20^{\circ}C$ for 24hrs. Also, when the seeds soaked in the aforementioned treatment, the rate of germination was increased at lower temperature than at $20^{\circ}C$, the optimal temperature. The percentage and the speed of seed germination depended on the kinds of pelleting binder and their concentration. It showed that the higher the concentration of the binder for seeds pelleting, the lower the percentage of seed germination. Among the pelleting binder, polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP) were the best binders for seed pelleting, because seed germination using those binder did not affect on the concentration of binder. On the other hand, Carboxymethy cellulose (CMC) and methyl cellulose (MC) severely inhibited the seed germination The germinability was also different arcording to the pelleting materials. Among the different 58 pelleting materials, kaoline alone, the mixture of bentonite and kaoline, the mixture of bentonite, calcium carbonate and diatomaceous earth #300 were found as the best pelleting materials for welsh onion seeds.

• Korean Journal of Plant Resources
• /
• v.20 no.5
• /
• pp.398-403
• /
• 2007

This study was carried out to enhance germination of the seed by different immersion temperatures(15, 20, 25, 30 and $35^{\circ}C$) and times(1, 6, 12, 18, 24, 36 and 48 hours) in Cryptomeria japonica that is a major plantation species in the southern Korea. Optimum germination temperature was between $20^{\circ}C$ and $25^{\circ}C$, and the lowest germination was observed at $35^{\circ}C$ Moisture absorption increased with increasing immersion time as a whole and was high at $30^{\circ}C$ and $35^{\circ}C$. Initial moisture absorption rate was highest at $30^{\circ}C$ whereas was lowest at $15^{\circ}C$ Moisture absorption rate constant was also high at $30^{\circ}C$ and $35^{\circ}C$, however, it was relatively low at $15^{\circ}C$ and $20^{\circ}C$ Germination was the highest when the seeds were immersed for 48 hours at $30^{\circ}C$. In mean germination time(MGT) the seeds immersed for 48 hours at $30^{\circ}C$ did not differ from non-treatment, while the others showed higher values than non-treatment. Consequently, germination could be enhanced and MGT was kept by optimum immersion temperature and time.

• Korean Journal of Medicinal Crop Science
• /
• v.8 no.2
• /
• pp.129-133
• /
• 2000

The experiment was conducted to increase germination of Agrimonia pilosa seeds, one of the natural medicinal plants, by various treatments, and also the effects of seed treatments on the growth of plants after sowing in field. The optimum temperature for germination of A. pilosa seeds was $20^{\circ}C$ after 20 days stratification duration at $4^{\circ}$, the germination rate than highest, 86% at that temperature. The stratification increased germination rate in all the temperatures treated, shortened $26{\sim}32$ days of germination period and promoted more 20% germination rate than the other treatments. Flowing water, low temperature, $GA_3$ and NaOCl were not effective for germination and high temperature reduced germination rate. At the early growth stage, the plant height was taller $3{\sim}4\;cm$, one or more leaves were increased by the stratification treatments, and also stratification increased the number of leaves per plants 10 or more leaves compare with other treatments at harvesting stages. By stratification, the flowering date was 5 days earlier, June 18th than other treatments due to earlier sprouting.

• Research in Plant Disease
• /
• v.24 no.2
• /
• pp.132-137
• /
• 2018

Melanose caused by Diaporthe citri is a serious disease on yuzu fruits. Moisture is the most important to infect after the pycnidiospores are released. In order to understand how dryness and moisture interruption affect on germination, the conidial suspensions on slide glass were dried for 0-48 h. For the moisture interruption experiment, moisture was supplied for 10 h then interrupted for 0-6 h depending on the treatments. Germinations on the treatments with longer than 15 h of dryness were less than 10%. And those with longer than 3 h of moisture interruption were less than 30%. Compared with other fungal phytopathogens, D. citri was xero-sensitive. Germination on yuzu feel medium was measured under 15, 20, 25, 30, $35^{\circ}C$ with 3, 6, 9, 12, 15, 18, and 24 h of wetness. The minimum wetness periods achieving 10% and 50% germinations were 4.5 and 13 h, and the optimum temperatures were $29.1^{\circ}C$ and $29.2^{\circ}C$, respectively. From the yuzu medium study, the regression equations to be required wetness period achieving 10% and 50% germinations on various temperatures were $Wh_{50%}=0.1093{\times}T^2-6.3762{\times}+106.08$ and $Wh_{10%}=0.082{\times}T^2-4.8025{\times}T+74.861$.

• Korean Journal of Plant Resources
• /
• v.13 no.3
• /
• pp.195-201
• /
• 2000

This study was conducted to investigate the suitable conditions for seed germination of Rosa davurica PALL. In the characteristics of the fruit and seed of Rosa davurica, fruit length and width was 1.3cm and 0.9cm , respectively, and seed number was eighty-nine. Artificial and low temperature storage at 4$^{\circ}C$ increased the rate of split achene pericarp until 46.6% and storage at 15$^{\circ}C$ incubator decreased the rate of split achene pericarp (10.5%). The rate of seed germination of split achene pericarp in control at 15$^{\circ}C$ was 90% and of non-split achene in GA$_3$100ppm at 15$^{\circ}C$ was 36.8%. Average germination day of split achene pericarp seeds in GA$_3$150ppm at 2$0^{\circ}C$ was 4.2 days and non-split achene pericarp in GA$_3$100ppm at 2$0^{\circ}C$ was 7.3 days. Seed germination was not different between various concentrations of GA$_3$ treatments in split achene pericarp seeds but the rate of germination was more reduced in high concentration of 200ppm. Only the treatments of GA$_3$ was increased germination rate at 4$^{\circ}C$ and immature seed of Rosa davurica.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2022.09a
• /
• pp.47-47
• /
• 2022

단자엽식물은 잎의 곡선과 직선의 조화가 아름다워 관상용으로 가치가 높은 식물이다. 이로 인해 자생 단자엽식물의 실내 관상 및 조경용 소재개발 연구가 활성화되고 있으나 재배에 대한 정보는 미비한 실정이다. 본 연구에서는 국내 자생 단자엽식물인 참김의털[Festuca ovina L. var. coreana (St.-Yves) St.-Yves], 청사초(Carex breviculmis R.Br.), 골풀[Juncus decipiens (Buchenau) Nakai]을 대상으로 몇 가지 재배 조건에 대한 생육 차이를 확인하였다. 국립생물자원관으로부터 분양 받은 세 종의 종자는 휴면 여부와 발아 적온을 확인하기 위해 종자를 페트리디쉬에 파종한 후 15, 20, 25, 30℃(명 또는 암조건) 및 25/15℃(16/8h, 명/암) 조건에 배치하였다. 재배실험은 유리온실에서 수행되었으며, 토양에 파종 후 4주간 육묘한 다음 균일한 생육상태의 묘를 7cm 포트에 이식하여 실험의 재료로 사용하였다. 토양실험은 시판 원예용상토와 마사토를 혼합하여 사용하였으며, 비율을 1:1, 2:1 및 3:1로 조성하였다. 차광 환경은 55, 75% 차광과 무차광 조건으로 달리 하였으며, 추비는 재배 6, 8, 10주차에 500, 1,000ppm의 하이포넥스(NPK 7-10-6) 용액을 엽면시비 하였다. 토양실험을 제외한 모든처리구에서는 원예상토 단용을 사용하였으며, 이식 후 12주간 재배하였다. 발아 실험 결과, 참김의털은 명조건에서 25/15와 15℃를 제외하고 발아율이 10% 이하로 조사되었으며, 암조건에서 온도가 증가할수록 발아율이 감소하였다. 청사초는 모든 온도에서 90%이상 발아하였으며, 암조건의 경우 발아율이 10% 이하로 조사되어 광발아성 종자로 확인되었다. 골풀은 실험이 진행된 30일 동안 모든온도에서 발아하지 않았으나, 재배실험을 위해 토양에 파종된 골풀은 파종 약3주 이후 발아가 관찰되었다. 재배실험 결과, 참김의털과 청사초의 경우 2:1로 혼합한 토양에서 엽수와 분얼수, 생체중 등이 가장 높게 조사되어 관상적 가치가 높았으며, 골풀은 3:1의 혼합토양에서 생육이 가장 우수하였다. 차광은 세 종 모두에게서 생육에 부정적인 영향을 미쳤으며, 청사초와 골풀은 500ppm의 하이포넥스를 시비하였을 때 생육이 촉진되었다.

• Korean Journal of Weed Science
• /
• v.6 no.2
• /
• pp.110-123
• /
• 1986

To know the ecological pattern of bulrush (Scirpus juncoides Roxb.) seeds and overwintreed stumps in germination and sprouting responses as affected by different temperature (7 trt.), light intensity (5 trt.), shading intensity (S trt.), light quality (specturm spectrum; 6 trt.), soil acidity (7 trt.), stump sizea (weight base; 5 trt.), and molding depth (6trt.), respectively, this serial studies were conducted by use of growth chamber, incubator, Wagner pot and petri-dish. Most efficient treatment was obtained from 25-$35^{\circ}C$ temperature, higher light intensity in 2-11 klux range, 95% shading intensity, clear and yellow film for seeds/clear and blue film for stumps, soil pH 5.53, 3-4g stump weight, 0-5% wxygen concentration, 1 ㎝ flooding depth for seeds, and 1-1.5cm molding depth for seeds/0.5-1.0 cm molding depth for stumps, respectively, among others.

• Journal of Korean Society of Forest Science
• /
• v.6 no.1
• /
• pp.19-23
• /
• 1967

The fungus which belongs to Alternaria has been isolated from "damping-off" infected seedlings of pitch pine (Pinus rigida Mill.) in the nursery (seed bed) of Chung Puk College, Cheong-Ju. The author has carried out this study regarding to (1) the effects of H-ion concentration on the growth of fungus, (2) the effects of temperature and humidity on the conidia germination, (3) the effects of temperature and humidity on the conidia formation. The summary of obtained in this experiments is as follows: 1. The suitable H-ion concentration for growth of isolated fungus is in the range of PH 4.0-9.5, the most suitable of it is PH 6.5-8.0. 2. The germination of the conidia is suppressed at below $0^{\circ}C$ or at $40^{\circ}C$ and above, the range of optimum temperature for the germination is $5^{\circ}C-35^{\circ}C$, the percentage of germination is highest at $20^{\circ}C$. And under relative humidities of 10% or below, the germination is suppressed, favorable relative humidities $85%{\cdots}100%$. 3. The formation of the conidia is suppressed at below $10^{\circ}C$ or at $34^{\circ}C$ and above, the favorable condition of the condia formation is as follows; Temperature; $22^{\circ}C-28^{\circ}C$ Relative humidity:85%-100%.