• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.5
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• pp.79-91
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• 2008

Research was initiated to investigate germination characteristics of creeping bentgrass (CB, Agrostis palustris Huds.). Seven varieties of CB were evaluated with different experiments. An alternative environment condition requiring for a CB germination test by International Seed Testing Association (ISTA) was applied in the Experiment I, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$(ISTA conditions). Experiment II was conducted under a room temperature condition of 5 to $25^{\circ}C$(natural conditions). In each experiment, data such as seed germinating vigor, early germination characteristics, germination speed and germination peak time were measured. Significant differences were observed in seed germinating vigor, early germination characteristics, germination speed and germination peak time. Seed germinating vigor was variable with different environments and varieties. It was 61.50 to 98.25% under ISTA conditions and 55.00 to 98.50% under natural conditions. There were considerable variations in early germination characteristics among CB varieties according to different conditions. Early germination characteristics indicated that all varieties were 1 to 4 days faster in germination under ISTA conditions, when compared with natural conditions. The germination speed, measured as days to seed germination of 70% and 90%, was much faster with Penn A-1, Penn A-4 and Penncross under ISTA conditions. But it was even faster with L-93 and Penncross when grown under natural conditions. Differences were also observed in germination peak time with varieties and growing conditions. It was 0.57 to 2.86 days under ISTA conditions and 0.74 to 1.74 days under natural conditions. Regardless of the environment conditions, the shortest variety was L-93 and the longest one T-1. Considering germinating vigor, early germination characteristics, germination speed and germination peak time, Penn A-1, Penn A-4 and Penncross were regarded as excellent varieties under ISTA conditions in terms of early establishment characteristics, while L-93, Penn A-1 and Penncross under natural conditions. These results suggest that an intensive germination test be needed prior to planting, for the early germinating vigor, germination speed and germination peak time. Also, a proper variety selection and comprehensive site analysis for the growing environmental conditions should be done before golf course construction.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.4
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• pp.447-453
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• 1986

Germination and seedling elongation of soybeans [cult. ‘Williams’] were measured at two temperatures (15 and 3$0^{\circ}C$), three seed water contents (30, 50, and 70%), and with and without polyethylene glycol-8000 (PEG) treatments. A split-plot design was used with four replications. Observations were made from two hours to eight days for each treatment combination. Seeds were soaked with 30% PEG solution with 0.2% thiram at 15 C for six days, rinsed with deionized water and dried at 25.5$^{\circ}C$ for three days. Ten treated or untreated seeds were placed on Whatman No. I (9 cm) filter paper in plastic petridishes to which sufficient deionized water was added to adjust seed moisture content to the desired level. The dishes were then sealed with parafilm and placed in a continuous programmed temperature chamber under dark condition. Seedling growth did not occur at either temperature when moisture content was 50 percent or less. Osmo-conditioning with PEG showed positive effects on seedling moisture uptake and seedling growth at 15$^{\circ}C$ but little effect at 3$0^{\circ}C$. Seedling moisture content increased rapidly early in the germination period and reached 60% at 15 C for 56 hours and at 3$0^{\circ}C$ for 28 hours. Seedling growth started when seed moisture reached a critical point of 60% at 15$^{\circ}C$, however, growth started after 20 hours of germination at 3$0^{\circ}C$. Seedlings of soybeans elongated more than four times faster at 3$0^{\circ}C$ than at 15$^{\circ}C$. Water uptake during germination was characterized by two phases in this experiment.

• Journal of Bio-Environment Control
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• v.24 no.3
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• pp.196-201
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• 2015

This study was conducted to seek optimum sowing time and pinching height for tender shoots production of moloheiya (Corchorus olitorius L.), which is a subtropical leaf vegetables, under an unheated greenhouse in the south of Korea. Seeds of moloheiya did not germinate at all at $10^{\circ}C$, but germinated well at $18{\sim}30^{\circ}C$ with 95.5~98.5% germination rate. Germination rate was 75.0% at $15^{\circ}C$, but germination speed was very low. The pods of moloheiya sown before April 28 was matured after late-August, but those sown after May 7 was not matured in that year. In tender shoot production and harvest frequency, treatment sown on April 14 was most plentiful among sowing time treatments, and showed marketable yield increase by 14~42% compared to other sowing times. Mean-while, pinching at 100cm high from soil surface was more effective than other heights for increment of branches and tender shoot. All above suggest that it was suitable to sow seed on mid-April and to pinch at 100cm high from soil surface for increment of tender shoots in the south of Korea.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.3
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• pp.23-38
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• 2015

The study was initiated with Kentucky bluegrass (KB, Poa pratensis L.) to investigate germination and early establishment characteristics of new cultivars for a practical application to turfgrass establishment such as parks, athletic field and golf course etc. Fifteen cultivars were evaluated in different experiments. An alternative condition for a KB germination test required by International Seed Testing Association (ISTA) was applied in Experiment I, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Experiment II was conducted under a room temperature condition of 5 to $25^{\circ}C$ (natural conditions). Seed germinating vigor, germination speed and germination peak time were measured in both experiments. Significant differences were observed in seed germinating vigor, germination speed and germination peak time. Seed germinating vigor was variable with different environments and cultivars. It was 75.25 to 89.50% under ISTA conditions and 75.75 to 90.25% under natural conditions. There were considerable variations in early germination characteristics among KB cultivars according to different environments. Early germination characteristics showed that all cultivars were 3 to 5 days faster in germination under ISTA conditions, when compared with natural conditions. The germination speed, measured as days to seed germination of 50% to 80%, was much faster with 'Midnight II', 'Excursion' and 'Midnight' under ISTA conditions. But it was faster with 'Midnight II', 'Excursion' and 'Odyssey' under natural conditions. Differences was also observed in germination peak time with cultivars and growing conditions. It ranged 5.94 to 14.88 days under ISTA conditions and 4.71 to 13.06 days under natural conditions. Regardless of the environment conditions, the shortest cultivars were 'Odyssey' and 'Midnight II'. The longest ones were 'Nuglade' under ISTA condition and 'Moonlight' under natural conditions. Considering germinating vigor, germination speed and germination peak time, 'Midnight II', 'Excursion', 'Midnight', and 'Odyssey' were regarded as excellent cultivars under ISTA conditions in terms of early establishment characteristics, while 'Midnight II', 'Excursion', 'Odyssey', and 'Courtyard' under natural conditions. These results suggest that an intensive germination test be needed prior to planting, for investigating the early germinating vigor, germination speed and germination peak time. Also, a proper cultivar selection and comprehensive site analysis for the growing environmental conditions should be done before turfgrass establishment such as golf course construction.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.5
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• pp.465-472
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• 1994

To improve the germination performance of rice seeds under suboptimal temperature, osmopriming with PEG-6000 was examined. Optimal PEG-6000 concentration to improve germination was 20% PEG-6000 solution, and rice cultivars used in this experiment were Sinunbongbyeo, Gancheokbyeo, Dongjinbyeo. The water content of seeds after soaking for 60 hours in the PEG solution is similar to that of seeds after soaking for 24 hours in the distilled water. Germination performance of the soaked seeds in the PEG solution was higher than that of the soaked seeds in the distilled water or the control, especially under suboptimal temperatures. Electrical conductivity of the soaked seeds in the PEG solution was lower than that of the soaked seeds in the distilled water or the control, and total dehydrogenase activity of the soaked seeds in the PEG solution was higher than that of the soaked seeds in the distilled water or the control. SDS-PAGE results of soluble protein from the embryos of seeds primed differently showed darker band in the seeds soaked in the PEG solution than the seeds soaked in the distilled water or the control at the 68 KD region. Also, band patterns of peroxidase and esterase of embryos soaked in the PEG solution were darked than that of embryos soaked in the distilled water or the control at the Rf 0.94 and Rf 0.87, respectively.

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.254-262
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• 2005

This experiment was carried out to select the desirable welsh onion (Allium fistulosum L.) varieties for the year-round cultivation in water culture. Ten varieties were tested, of which five were Korean's and the other five were Japanese's. The germination percentage and germinative energy of most varieties we.e more than 90 percent. The fastest germination speed were 'Geumjanguedaepa', 'Gujopa' and 'Fuyune'. The best growth of the varieties were 'Geumjanguedaepa' and 'Tokyokuro' in spring, 'Geumjanguedaepa', 'Gujopa', 'Saesaeksisilpa' and 'Tokyokuro' in summer, 'Geumjanguedaepa', 'Gujopa' and 'Tokyokuro' in fall, and 'Geumjanguedaepa', 'Midorigawa' and 'Tokyokuro' in winter cultivation. Suitable varieties of growth fur all seasons were 'Geurnjanguedaepa' and 'Tokyokuro', though 'Gujopa' was good in summer and fall. In the view of nutritional content, nitrate content was significantly lower in 'Geumjanguedaepa', 'Tokyokuro' and 'Fuyune' than that in'Saesaeksisilpa' and 'Starziam'. Vitamin C content was higher in 'Aqua Green', 'Midorigawa' and 'Seoulbaekpa' than that of the others. Pyruvic acid content was the highest in 'Geumjanguedaepa' and 'Suckchanguedaepa'. Based on the above results, 'Geumjanguedaepa', 'Gujopa' and 'Tokyokuro' were selected as the most desirable young welsh onion varieties for the year-round water culture.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.1
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• pp.96-102
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• 1981

Effect of shifting planting-time and different nitrogen on the yield and characteristics of plant growth in safflower, Chrthamus Tinctorius L. This study was carried out to investigate the optimum sowing-date and the ecological variations due to differerent amount of nitrogen for safflower on the Experiment Farm of Sang Ji college from March 17 to may 16, 1980, sowing seeds with an interval of 10 days and the amount of nitrogen were applied in 5 levels (Non, half-standard, standard, one and half-ordinary amount-No and twice amount). The triple super phosphate and pottassium chloride were applied only in standard amount. The results obtained are summarized as follows; 1. As sowing-date was delayed, the germination-ratio decreased, germinating speed increased, and the time required to attain the most vigorous germinating stage and days required for germination shortened. 2. Plant height, number of branch and stem diameter showed a decrease as sowing-date was delayed. And at the same time, plant height and number of branch showed a tendency to increase as amount of nitrogen was increased. 3. As sowing-date was delayed, the number of pods and the weight of 1000-grains were decreased. Moreover, the earlier sowing-date was, the more it increased. And as amount of nitrogen was increased, number of pods and yield also were strikingly increased. 4. The fresh-weight of flower with orange and orange-red colour, as influenced by the different sowing-date, was found out to be largest at the begining of April. But increasing amount of nitrogen did not show influence upon the promotion of flowering. 5. Judging from the results reported above, the optimum sowing-date of safflower seemed to be the begining of April; also the culture of safflower seemed to be the effects of much amount of nitrogen.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.3
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• pp.280-285
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• 1983

The effect of pH and calcium on the growth of subterraneum clover (Trifolium subteraneum L.) varieties Dwalganup, Mt. Barker, and Tallarook was determined in solution culture. At pH 4.5 and below all components of growth except germination percentage were adversely affected. The effect of lower pH was improved at high calcium level(3.0mM). There was a varietal difference in response to low pH. Mt. Barker showed the highest percent germination and produced the longest radicle length, whereas the greatest value in top dry weight, root dry weight, root length and number of laterals was obtained with Tallarook.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.281-286
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• 2007

Surface sowing method is one of the important methods to establish seeds in mountainous area. Seed-coating technique in the surface sowing method is increasing interest in the world today. An experiment was conducted to develope a technique for pelleting seeds of grass in woodland. Results showed that the seed pelleting was a useful technique than coating seeds because the pelleting reduced coating time and increased amounts of coated seeds per hour. As lowering temperature and reducing time for drying, germinating energy and germination rate of pelleted seeds were improved. And emergence, establishment and early growth of pelleted were also improved in pasture under woodland. Based on this study, we could conclude that the seed pelleting is a useful technique for the establishment and early growth of grass under woodland.

• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.231-236
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• 1998

Carrot cotyledon explants cultured on MS medium with 1 mg/L 2,4-D were transferred to a hormone-free solid medium overlaid with filter paper in order to elucidate the effect of simple physical treatment on the development and germination of somatic embryos. Transfer of the explants cultured for one week on MS basal medium overlaid with 3 sheets of filter paper on to MS basal medium increased somatic embryo production 2-39 times over the one week culture on medium without filter paper overlay. Maturation and germination of somatic embryos was more prominent on medium overlaid with filter paper than on medium without filter paper. The explants cultured for one week on filter paper overlay added with liquid medium showed prominent decrease in somatic embryo formation compared to filter paper overlay only. It is suggested that the filter paper overlay affected the moisture environment of the somatic embryos developing on it.