• Korean Journal of Plant Resources
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• v.14 no.3
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• pp.251-258
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• 2001

Predehydration effects for cryopreservation in -196$^{\circ}C$ liquid nitrogen were studied in Korean native Aquilegia buergeriana var. oxysepala Kitamura and A. flabellata var. pumila Kudo seeds. Aquilegia species seeds were adjusted to moisture contents between 3.2 and 9.7% by air dry treatments. Seeds were placed in paper envelopes after submerged in liquid nitrogen and rewarming in 38 $^{\circ}C$ water. Seeds moisture contents by duration of drying were identified as controlling factors in the survival of Aquilegia species seeds for cryopreservation. Aquilegia species seeds having approximate 5% moisture content were able to withstand cooling to -l96$^{\circ}C$. Undehydrated seeds of Aquilegia buergeriana var. oxysepala Kitamura after being cryopreservated in liquid nitrogen have a 10.9% of moisture content and show 52.5% in germination. But, Aquilegia buergeriana var. oxysepala Kitamura seeds dehydrated by drying for 60 min. to have 6.0% of moisture content before cryopreservation show 84.7% in germination test. Properly dehydrated seeds after being stored in liquid nitrogen showed over 60% in germination rate and also shows an uniform sprouting time,11~13 days in average. Any morphologically deformity in germinating beds has not been observed. Results from this study suggest that Aquilegia species seeds can cryopreservation in liquid nitrogen if the seed moisture content is controled by a proper amount of dehydration.

• Korean Journal of Agricultural Science
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• v.11 no.2
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• pp.201-206
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• 1984

In order to obtain an easy and effective scarification method for stimulating the seed germination of Korean lawn grass (Zoysia japonica Steud.), sodium hypochlorite (SH), which is used as a commercial bleach and a seed disinfestant, was tested as a chemical scarifier instead of potassium hydroxide (KOH). 1. Scarification with 4% SH for 2 hours followed by 8-hour water-rinse stimulated seed germination more effectively compared to generalized scarification method by KOH treatment. 2. The degree of stimulating seed gemination was different by the various water-rinsing time after SH treatment, but the optimum rinsing time was about 4 to 8 hours. 3. GA treatment after SH scarification stimulated seed germination. However, the stimulation effect of GA was not showed in non-scarified control. 4. SH treatment significantly decreased the percentage of the infection of microflora in seed samples compared to KOH treatment. 5. Under practical seed scarification conditions for stimulating germination of Korean lawn grass seed. SH may have advantages over KOH with respect to ease of preparing, securing and handling.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.340-350
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• 1995

This study was conducted to obtain the basic information for the salt stress of Italian Ryegrass. Fourteen cultivars including 7 diploid and 7 tetraploid were subjected to eight levels of NaCl treatments during germination and early seedling stages. Germination percentage decreased remarkably over 300mM of NaCl concentration. The NaCl concentration that inhibited germination of 50% of the viable seeds in the cultivars was 344mM. The decreasing degree of germination percentage was higher in tetraploid type than in diploid type. The varietal difference of germination percentage was shown to be very conspicuous as the NaCl concentration increased. Top dry weight in 166 mM NaCl was decreased by 50% compared with that of control. Inhibition of NaCl appeared to be more in early seedling stage than in germination stage. Top dry weight of cultivars in NaCl stress was not related with germination capacity. Root dry weight in 148mM NaCl decreased by 50% compared with that of control. Root dry weight of cultivars was significantly correlated with top dry weight in NaCl treatment. Top dry weight and root dry weight was higher in diploid type than in tetraploid type at each 8 levels of NaCl concentration. Total chlorophyll content increased with the increase of NaCl concentration and the degree of increase in chlorophyll a was higher than that in chlorophyll b. Chlorophyll a was higher in tetraploid type than in diploid type. But chlorophyll b was higher in diploid type than in tetraploid type. There were significant correlation between chlorophyll a and top dry weight of cultivars over 150mM of NaCl concentration. Free proline content accumulated remarkably in NaCl treatment compared with control and was not difference between diploid type and tetraploid type. Free proline content of cultivars was not related with top dry weight in NaCl treatment.

• Journal of Korean Society of Forest Science
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• v.80 no.2
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• pp.237-245
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• 1991

Artificial acid rain (pH 3.0, 4.0 and 5.0) was treated on thd seeds of 5 coniferous species to examine its effects on germination and seedling growth. Artificial acid rain was prepared by diluting sulfuric acid with ground water and ground water(pH 6.5) was used as control. Artificial acid rain was sprayed to seeded pots three times per week. About 5mm of artificial acid rain was treated each time from early April to early October, 1990. Germination, seedling establishment and seedling growth were measured and compared among the treatments. The results were summarized as follows ; 1. Germination, seedling establishment rate of Larix leptolepis and Picea koraiensis were highest on the pH 5.0 plot whereeas those of Abies holophylla and Cryptomeria japonica were highest on the control plot, and those of Abies koreana showed the highest value on the pH 3.0 plot. 2. The differences in seedling height growth of Larix leptolepis was signif- icant at 1% level among the treatment levels of acid rain, whereas those of other species were not significant, Height of Larix leptolepis, Abies koreana and Picea koraiensis were highest on the pH 4.0 plot, and that of Abies holuphylla and Cryptomeria japonica were highest on the pH 5.0 plot. 3. Seedling dry weight of Larix leptolepis and Abies holophylla differed significantly at 1% level among the treatments, but those of other species did not differ. Highest seedling dry weight of Larix leptolepis and Abies koreana were observed on the pH 4.0 plot, and that of Abies holophylla and Cryptomeria japonica did on the pH 3.0 plot, and that of Picea koraiensis did on the control plot. 4. With decrease of pH value of artificial acid rain, the number of injured needle and injured individual tended to be increased, obviously. 5. The differences of soil acidity were highly significant among the treatments for all species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.1
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• pp.59-63
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• 1988

Freshly harvested and depulped Korean ginseng seeds were subjected to the seed treatment of removing endocarp plus surface sterilization with sodium hypocloride, surface sterilization only, and nontreated control. These seeds were stratified at temperatures of 5$^{\circ}$, 10$^{\circ}$, 15$^{\circ}$ and 20$^{\circ}C$ for 20, 40, 60, 80 and 100 days. Embryo growth of the ginseng seeds of which endocarp was removed was most rapid in each stratification temperature and that of sterilized seeds was slower than unsterilized seeds after 80 days stratification at 15$^{\circ}$ and 20$^{\circ}C$. About 15$^{\circ}C$ was an optimal stratification temperature for embry growth in ginseng seeds. Chilling treatment at 5$^{\circ}C$ for 100 days was needed for better germination of dehisced ginseng seeds. An optimal germination temperature for the ginseng seed following chilling treatment was about 15$^{\circ}C$.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.13-13
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• 2010

복주머니난(Cypripedium)속 식물은 우리나라에 광릉요강꽃을 비롯하여 털복주머니난, 흰털복주머니란, 복주머니란, 노랑복주머니란등 5종이 분포하는 것으로 알려져 있다. 광릉요강꽃과 털복주머니란 두 종은 환경부에서 지정한 멸종위기 식물 1급과 2급에 지정되어 보호를 받고 있고 나머지 3종은 제도적으로도 보호를 받지 못하는 실정이다. 본 연구에서는 충분히 성숙한 털복주머니난과 복주머니난의 종자에 NaOCl처리를 하여 발아율을 향상시킬수 있었는데 이러한 전처리가 발아에 미치는 원인에 대한 연구를 실시하였다. 털복주머니란의 무균적인 종자발아는 1.0% NaOCl 처리구에서 70% 이상의 종자발아율을 보였으며, POM배지가 MS배지보다 신초분화가 양호했다. GA3와 활성탄(Activated charcoal)의 혼합첨가는 신초증식에 효과적이었다. 신초분화 후 저온처리는 신초의 증식 율을 증가시켰다. 한편 NaOCl 농도(0, 1, 3, 5, 10%)와 NaOCl 처리시간(0, 5, 15, 30, 45, 60분)에 따라서 복주머니란의 종자발아를 확인한 결과, NaOCl 1%를 30분간 처리하였을 때 가장 높은 발아율을 나타냈다. NaOCl을 처리하여 종자의 종피상태를 SEM과 TEM으로 관찰한 결과 NaOCl의 처리는 종피 세포벽의 부분적 해리 및 작은 구멍을 만들게 하였는데 이러한 종피의 물리화학적 변화가 물과 양분의 이동을 원활히 하여 종자의 발아를 촉진하는 것으로 사료된다. 복주머니난의 신초분화에 미치는 casein과 활성탄의 효과를 알아본 결과 casein 200 mg/L와 활성탄 200 mg/L를 혼합 첨가한 실험구에서 가장 높은 신초분화율을 보였다. 토양순화 후 생존률은 극히 저조했으며 30 개체중에 5 개체만이 다음해 어린동아를 싹틔우는 것을 확인 하였다. 본 결과들을 종합하여 보면 멸종위기식물, 특히 털복주머니란과 복주머니란의 조직배양을 통해서 서식지외 보존방안(기내증식)에 관해 가능성을 제시하였다고 생각되어 진다.

• Journal of Korean Society of Forest Science
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• v.3 no.1
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• pp.43-46
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• 1963

1. Almost complete death of pitch pine seeds resulted when presoaked in 0.5 per cent ethyleneimine solution without neutralization. When neutralized with 1 N HCL, quite high germination percentages were obtained even with 2 per cent solution. 2. When presoaked in 0.25 or 0.50 per cent ethyl methanesulphonate, there was a delay in germination, but the germination per cent rapidly increased from about the 10 th day from sowing. 3. Basing upon the time when the germinative energy of control seeds was reached, the comparative lethal doses (concentrations) among mutagenic chemicals and radiations were investigated (Table 1).

• The Korean Journal of Ecology
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• v.17 no.3
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• pp.367-378
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• 1994

The germination responses of Echinochloa crus-galli (L.) Beauv. seeds to temperature were examined under the various thermal conditions. While almost all the seeds tested did not germinate immediately after collection, almost of the seeds which were stored for 7, 10, and 17 months showed very high germination percentages (85-95%) at their own constant temperatures between $16^{\circ}C\;and\;40^{\circ}C$. The total thermal time which was required for germination(10-70%) of Echinochloa crus-galli seeds ranged from 539Kh(degree Kelvin X hour) to 1,279Kh in accordance with the distribution function of thermal time, $F({\Theta})=1-[3D^{-3}({\Theta}-m+D)+1]^{-1/2}$, where m is 935Kh and D is 555Kh. Moist chilling treatment at $2^{\circ}C$ for 20 days increased the final germination percentage as well as the germination rate. In the increasing temperature(IT1 regime, E. crus-galli seeds started to germinate at $12^{\circ}C$. and showed greater germination rate with increasing temperatures, with the final germination percentage of 80%. On the other hand, in the decreasing temperature(DT1 regime, the seeds began to germinate at $12^{\circ}C$(10% germination) with the final germination percentage of 20%. An induced dormancy occurred at $4^{\circ}C$ in the DT regime.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.4
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• pp.281-288
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• 2008

Seed priming is an useful technique for increasing germination and early establishment of seedlings. In this experiment, the conditions for priming of pasture seeds (tall fescue, orchardgrass, alfalfa and white clover) have been optimized to ensure an early germination and more uniform growth of seedlings. The experiment was conducted in a split plot design with three replications. The main plots consisted of four different priming duration such as 2, 4, 6 and 8 days for grass, and 1, 2, 3 and 4 days for legume. The subplots were consisted of three priming temperature such as 10, 15 and $20^{\circ}C$. Effects of priming duration and temperature were different in all four pasture seeds examined. The germination of primed grass (tall fescue and orchardgrass) was the highest on 6 days in priming duration, whereas that of legume (alfalfa and white clover) was the highest on 2 days. In priming temperature, the germination of primed orchradgrass increased as decreasing temperature, however that of legume increased as increasing temperature. These results suggest that seed priming induced earlier and higher germination for all four pastures tested. However, the degree of priming effectiveness on pasture species depending on the priming duration and its temperature.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.3
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• pp.319-323
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• 2013

Red rice (Oryza sativa L.) is one of the most important weeds in most of rice cultivating area. Seed germination related experiments were done to evaluate germination percentage, emerging speed, and initial growth by seeding depth and soil moisture level. The four experiments, 1) temperature, 2) seeding depth, 3) soil moisture level, and 4) seeding position, were done with combination each other on phytotron for germination and seedling growth related experiments of redrice. The treatments levels were: 1) Temperatures were 20/15(Low), 25/20 (Mid.), $30/25{\circ}C$ (High), (day/night), 2) seeding depths were 0, 3, 6 cm, 3) soil moisture levels were 25, 35, 45, 55, 65% (VWC, %), and seeding position were furrow, ridge, ridge-top. The germination percentage and germination speed of red rice were higher and faster than Daeanbyeo in low temperature. Yoeongcheon redrice of seed germination percentage and seedling length was more vigor than Hapcheon red rice. Red rice was not germinated on 6 cm seeding depth until 11 days after seeding except high temperature treatment. Germination percentage increased with increasing soil water percentage in low temperature, however it was greatest in 45% in high soil moisture level between 25% to 65% in low temperature. Seed germination percentage and seedling length were not significantly different among the soil water level in mid- and high temperature levels. In conclusion, red rice could germinate in top soil (<6 cm) in mid- and high temperature range, so we might be control red rice by spraying herbicide after germination of red rice combined with delayed rotary cultivation.