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Development and Preliminary Evaluation of a Leukocyte Removal Aptamer Filter (압타머를 이용한 백혈구제거필터의 개발 및 예비평가)

  • Lee, Yangwon;Jung, Eun-Suk;Choi, Kyoung Young;Kim, Myung Han;Kwon, So Yong;Cho, Nam Sun;Kim, Jin Sook;Park, Han Jeong;Han, Byoung Don;Yoon, Soo-Young
    • The Korean Journal of Blood Transfusion
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    • v.23 no.2
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    • pp.107-114
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    • 2012
  • Background: Leukocyte reduction filters are widely used to prevent transfusion reactions caused by leukocytes in blood components. Commercial filters are not sufficient for removal of leukocytes for prevention of transfusion associated graft-versus-host disease; therefore, irradiation of blood components was performed using expensive equipment. Techniques using an aptamer substituted for antibody have been developed and are available in clinical areas. The purpose of this study is to develop the aptamer filter system and to evaluate its efficiency and the possibility of its clinical application. Methods: Aptamers targeted to CD45 were selected by the Postech Aptamer Initiative. The aptamer filter in which aptamers attached to beads were bound to leukocytes and removed by magnetic field was developed. Filtration of 14 units of leukoreduction-red blood provided by Korean Red Cross Blood Services was performed using aptamer filters. Leukocyte removal rate and red cell recovery rate were evaluated and bacterial culture was performed. Results: After filtration using the aptamer filters, 45.6% of leukocytes were additionally removed and the red cell recovery rate was 92.8%. No growth in the bacterial culture was observed. Conclusion: In order to apply the cell depletion technique utilizing an aptamer to blood filter system, we developed and evaluated the aptamer filter system. Through improvement of the binding efficiency of the aptamer and the filtering process, and application of the various aptamers for other different cells, we suggest that this technique can be applied in the clinical area, such as a substitution for the irradiation process for TAGVHD prevention.

The Analysis of Future Promising Industries of Busan and Marine Policy in the Era of the Northern Sea Route (북극항로 시대에 대비한 부산지역의 미래성장 유망산업 및 정책 평가에 관한 연구)

  • Ryoo, Dong-Keun;Nam, Hyung-Sik
    • Journal of Korea Port Economic Association
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    • v.30 no.1
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    • pp.175-194
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    • 2014
  • Because the thawing of the Arctic ocean is slowly accelerating due to global warming, recently exploring resources in Arctic ocean and transporting resources by using the North Pole route have been getting spotlight. Since the original route transported by the Suez Canal from Korea to Europe could be shorten about 8,000km in distance(decreased about 38% compared to the original route), which means shortening about 10 voyage dates, it is expected to bring huge logistics cost reduction. Once the North Pole route is commercialized successfully, it would be one of the most important variables that affects future of Busan port and guides for economic development of Busan. Therefore, the purpose of this study is to analyze Busan port and the economic growth of Busan area by researching promising industry, based on the effect of freight transporting by the Northern sea route on the economy of Busan. For this study, questionnaire surveys and interviews were conducted for 64 people of experts in the shipping and port industry, relevant government, and academics. The survey finding shows that port logistics industry is a promising business in Busan in terms of its growth and competitiveness. It is necessary to develop feeder network facilities that prepare for commercialization of the Northern sea route as a short and medium term plan and provide professional manpower training in polar regions. Ship supply business would also play an important role. It is identified that revitalization of shipbuilding and ocean plant industry should be done in terms of Arctic business. With regard to the fishery industry it is found that modernization of fishery ship and development of fishery equipment used in polar areas should be carried out.

Analysis of Polar Region-Related Topics in Domestic and Foreign Textbooks (국내외 교과서에 수록된 극지 관련 내용 분석)

  • Chung, Sueim;Choi, Haneul;Choi, Youngjin;Kang, Hyeonji;Jeon, Jooyoung;Shin, Donghee
    • Journal of the Korean earth science society
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    • v.42 no.2
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    • pp.201-220
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    • 2021
  • The objective of this study is to increase awareness and interest regarding polar science and thereby aid in establishing the concept and future direction of polar literacy. To analyze the current status, textbooks based on the common school curriculum pertaining to polar topics were reviewed. Six countries that actively conduct polar science, namely Korea, France, Japan, Germany, the United States, and the United Kingdom, were chosen. Subsequently, 402 cases in 110 science and social studies (geography) textbooks of these countries were analyzed through both quantitative and qualitative methods. Based on the obtained results, the importance of polar research in geoscience education and the need for spreading awareness regarding polar research as an indicator of global environmental changes were examined. It was found that the primary polar topics described in the textbooks are polar glaciers, polar volcanism, solid geophysics, polar infrastructure, and preservation of geological resources and heritage. This demonstrates that the polar region is a field of research with important clues to Earth's past, present, and future environments and is also a good teaching subject for geological education. However, an educational approach is needed for systematically laying emphasis on polar research. The implications of this study are manifold, such as the establishment of a cooperative system between polar scientists and educators, extraction of core concepts for polar literacy and content reconstruction, discovery of new polar topics associated with the curriculum, diversification of forms of presentation in textbooks, and development of an affective image that is based on correct cognitive understanding. Furthermore, through the continuance of polar topics in textbooks, students can improve their awareness regarding polar literacy and polar science culture, which in turn will serve as the driving force for sustainable polar research in the future.

The Evaluation of Carbon Storage and Economic Value Assessment of Wetlands in the City of Seoul (서울시 습지지역의 탄소저장 및 경제적 가치 평가에 대한 연구)

  • Choi, Jiyoung;Oh Jongmin;Lee, Sangdon
    • Ecology and Resilient Infrastructure
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    • v.8 no.2
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    • pp.120-132
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    • 2021
  • The ecosystem and landscape conservation areas of Seoul were designated according to the Natural Environment Conservation Act and the Natural Environment Conservation Ordinance. With the adoption of the "Rapid Assessment of Wetland Ecosystem Service (RAWES)" approach and the "wetland ecosystem service" for the Ramsar Wetland City Accreditation at the 13th Meeting of the Conference of the Contracting Parties to the Ramsar Convention on Wetlands in 2018, the need for data evaluating wetland ecosystem services has become a necessity. Therefore, in this study, we selected five wetlands from the ecosystem and landscape conservation areas in Seoul, having high ecological conservation values, and evaluated their carbon sequestration and economic value assessment using the InVEST model, which is an ecosystem service evaluation technique. The evaluation results for carbon storage in each wetland are as follows: Tancheon Wetland: 3,674.62 Mg; Bamseom Island in the Hangang River: 1,511.57 Mg; Godeok-dong Wetland: 5,007.21 Mg; Amsa-dong Wetland: 7,108.47 Mg; and Yeouido Wetland: 290.27 Mg. Particularly, the Tancheon Wetland showed the lowest carbon sequestration of 1,130.37 Mg, as compared to the results acquired in 2013, of 4,804.99 Mg. When the average effective carbon rate of $16.06 (US) was applied to the decreased carbon sequestration value, a loss of $15,910.58(US) was calculated. Furthermore, if the average social cost of carbon ($204 (US)) is considered, which includes the impact of climate change on productivity and ecosystems, the total loss is equivalent to $202,101.97 (US). This study aims to examine the natural resource value of urban wetlands by evaluating selected major wetlands in Seoul. This study can be utilized as basic data to plan for the protection and management of the ecosystem and landscape conservation areas. Additionally, because wetland value assessment is considered essential, the results of this study can be used in future research to provide measures for evaluating ecosystem services in the Ramsar Wetland City Certification System. Moreover, this study can be utilized for selecting important wetlands as Ramsar sites, and to raise awareness about the significance of conserving urban wetlands, and for expanding international exchange among the Ramsar Wetland sites.

Tradition and Identity of Korean Mime (한국 마임의 전통성과 정체성 - 기원, 역사, 특징 -)

  • Kim, Ik-Doo
    • (The) Research of the performance art and culture
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    • no.18
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    • pp.5-46
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    • 2009
  • The origin of Korean mime is traceable to sacred actions of prehistoric age. There are materials about mime of this age in the archeological materials of this age, oral literature/myth, and written literature about this age. There were traces of the most original form of mime in primitive ritual of tribal nation age. The mimes of Samkooksidae/Tree Nation Age of Korea were presented to forms of Kamoobekhee(歌舞百戱)/Sanakbekhee(散樂百戱). We can discover traces of mime of this age in Hosunmoo(胡旋舞), Gwangsumoo(廣袖舞), Kweraehee(傀儡戱), Keeak(伎樂), Kummoo(劍舞) Muaemoo(無㝵舞), and so forth. Especially, Keeak in Beckjae was mask mime of Buddhistic contents. We can recognize that secular theatres were more diversified and strengthened than sacred thaetres in Nambukgooksidae/South-north Nation Age. According to these changes, there were many changes in the mime of this age. We can concretely find traditions of mime of this age in Cheryongkamoo(處容歌舞), Hwangchanmoo(黃倡舞), Taemyun(大面), Wuljen(月顚), Sodok(束毒)', Sanyae (狻猊), and so forth. Mimes of Koreasidae/Korea Age take diverse forms of puppet play, mask play, dance play. Established traditional mimes as Cheryongkamoo(處容歌舞) were widely disseminated in society. And dance plays of mime form as Hunsundo(獻仙桃), Pokurak(抛毬樂), Yunhwadae(蓮花臺)' were imported from Song Nation of China. Mime of Chosundidae/Chosun Age were developed with changes of theatre that were divided into Kyusickjeehee[規式之戲] as Kwangdae(廣大), Ser-in(西人), Joojil(注叱), Rongryung(弄鈴), Kendoo(斤頭) and Sohakjihee[笑謔之戱] as Soochuk(水尺) Sengkwangdae(僧廣大). Styles of theatre in this age were specialized into mudangkuk, Pungmoolkut, Inhyunguk/Puppet play, Talnoree/Mask paly, Pansori, Kungjoong Kamuakguk. According to this changes, mime of this age were specialized into diverse aspects. Korean mime were specialized into Kutnorum-formed mime, Inhyungnorum-formed mime, Jabsaeknorum-formed mime, Talnorum-formed mime, Kungjoongmuyong-formed mime, Pansori-formed mime, and so forth.

Freud's Moses-study and the Principle of Mythological Hermeneutic: Its Political Theological Interpretation Through Jan Assmann's Theory of Cultural Memory (프로이트의 모세-가설과신화해석학의 원리: 얀 아스만의 문화적 기억이론을 통한 정치신학적 해석)

  • KIM, JIN
    • Journal of Korean Philosophical Society
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    • no.119
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    • pp.129-159
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    • 2017
  • The study of Freud's Moses and the background of the establishment of monotheism has become a subject of attention in the new atmosphere of the spreading of political theology and the recent rediscovery of Egyptology. This paper examines the publishing background and intentions of Freud's last book, Moses and Monotheism. And it will emphasize the fact that the Moses-Egyptian theory and his criticism of monotheism hid political theological intentions to prevent the spread of anti-Semitism in Nazi Germany. According to the Egyptologist Jan Assmann, there is a difference in that Moses' Judaism is monotheism, but Akhenaten's Aton-religion is a cosmotheism, and while Freud emphasizes Moses the 'historical figure' of that name, Assmann refers to Moses as a 'mnemohistorical figure.' Just as Freud said that the source of Jewish hatred is in Moses himself who established monotheism, Assmann argues also, monotheism is based on the so-called "Mosaic distinction" that distinguishes between true religion and false religion, thus it is possible to dismantle oppression and violence through the abolition of the Mosaic distinction. Assmann estimates that Freud had a clear stance to stop the spread of anti-Semitism as "the most explicit opponent of the Mosaic distinction." While anti-Semitic hatred spread to Christians in the Nazi era, Freud regards the real founder of Christianity, a jew Paul, as both a "Judaism destroyer" and a "successor to Judaism." At this point, Taubes began to see Paul's theology from a political theological point of view, and Assmann succeeded it. The "historical Moses" described by Freud are not "Prophet Moses" but "Moses as lawmaker and political reformer", and Jewish hatred has arisen in his distinction. Thus, Freud's monotheistic criticism as "disintegration by historical reduction"(Nietzsche) has political theological power. Just as Taubes interpreted Paul as a political theologian, Assmann found political theological elements in Freud's criticism of monotheism.

A Bibliographical and Literary Research on the Xinxu(新序) of the Published edition in Joseon (조선간본(朝鮮刊本) 『유향신서(劉向新序)』의 서지·문헌 연구)

  • You, Sueng-hyun;Min, Kuan-dong
    • Cross-Cultural Studies
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    • v.51
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    • pp.257-257
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    • 2018
  • Xinxu(新序) was published in Korea by 1492. Among the existing editions, the editions that can confirm the realities are the collections of Keimyung University, the Korean Studies Central Research Institute, Kyonggi University, Hujodang(後彫堂), and the National Assembly Library of Japan. The Keimyung University's precious book is the 'first published book', and the old book is the 'later published book' which covers pages 69-70 and 71-72 of the first published book. It is the 'later published book' that has the same side inscribed. The second books, the Central Research Institute of Korea Studies and the Kyonggi University Collection are the first published books, and the Hujodang and the National Assembly Library of Japan are on pages 9-10, 63-64, 87-88, 107-108. The corresponding side is the 'later published book'. Comparing the editions, it can be concluded that the existing editions of the previous editions have been withdrawn two times, and in the latter editions, the existing editions of four editions can also be confirmed to have been edited three times. In this paper, the literature based on the existing editions was studied and features of the Korean edition were presented. First, we examine the types of paragraphs. In principle, the text is composed of '11 lines and 18 characters', but on the actual version, the number of characters is shown in the table. In the Korean edition of the Joseon dynasty, a blank space appears in the original text. The erroneous letter in the Joseon book was identified the reason for the error was explained in detail.

Screening of cryoprotectants (CPAs) for cryopreservation in the Nitzschia sp. of marine microalgae (해양 규조류 Nitzschia sp.의 초저온동결보존을 위한 보존제의 영향 분석)

  • Lee, In Hye;Jeon, Ji Young;Kim, Kyeung Mi;Kang, Myung suk
    • Journal of Plant Biotechnology
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    • v.45 no.4
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    • pp.400-408
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    • 2018
  • Biodiversity has continued to degrade in the $21^{st}$ century due to global warming occasioned by destruction of the environment around the world.. The Nagoya protocol places Korea in a unique position to effectively develop and protect its domestic genetic resources. Microalgae under study in this research contains large amount of antioxidant substances such as beta carotene and astaxanthin, that can be used as biological resource owing to the large amounts of biomass that can be secured through photosynthesis. However, it is difficult to preserve it since cryopreservation method used for long-term preservation is yet to be developed. A basic study for long term cryopreservation was carried out on Nizschia frustulum and Nitzschia amabilis which belong to marine diatoms. As cryoprotectants (CPAs), glycerol, DMSO, and methanol which penetrate into cells were prepared at 5%, 10%, and 15% concentrations each, in case of methanol, it was tested at concentrations of 5%, 10% and 12% by its nature. Two kinds of microalgae, N. frustulum and N. amabilis, were diluted with $10^2$, $10^3$ and $10^4cells\;ml^{-1}$, respectively. The highest survival rate was shown at12% concentration of methanol, and the figures were $6.94{\pm}0.31%$ in N. frustulum and $8.85{\pm}0.16%$ in N. amabilis. As a result of 3 weeks cultivation of thawed microalgae after freezing, the result is shows that N. frustulum increased about 10 times faster and N. amabilis increased about 12 times the original concentration.

A Study on a Drainage Facility of the Western Shore in Wolji Pond (월지(月池) 서측 호안의 출수시설(出水施設)에 관한 고찰)

  • Oh, Jun-Young
    • Korean Journal of Heritage: History & Science
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    • v.51 no.3
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    • pp.72-87
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    • 2018
  • This study highlights a drainage gate and a ditch, which existed around the whole area of the western shore of Wolji Pond(月池) and focuses on a possible connection between the drainage facility on the western shore and the historical drainage system of Wolji Pond. Specifically, it primarily considered locations and the form of a drainage gate, the relationship between northwestern ditch of Wolji Pond and the drainage gate, and the establishment period and the character of the drainage facility on the western shore. The drainage gate found in excavation in 1975 is determined as the same facility as Surakgu(水落口) recorded on an actual measurement drawing, 1922. Therefore, it is highly probable that there were already the drainage facility in the western shore of Wolji Pond before the 1920s. The drainage gate constructed by processing rectangular stones has four drainage holes for controlling water level. The way of the drainage through the drainage holes is the same as that of the northern shore of Wolji Pond. From a cadastral map drawn in 1913, it is found that the ditch existed in northwest of Wolji Pond. The ditch was proximate to the drainage gate and shared the same axes. Hence, the ditch and the drainage gate are determined as a organic facility connected to the drainage system of Wolji Pond. In particular, the ditch existed in northwest of Wolji Pond is the basis for judging that the drainage facility in the western shore were established before the 1910s. Water flowed in through drainage holes of the drainage gate is drained into the northwest of Wolji Pond, through the ditch. The establishment period and the intention of the drainage facility on the western shore can be interpreted in two aspects. First, they might be 'a agricultural irrigation facility in the Joseon era', given that Wolji Pond was recorded as a agricultural reservoir, and that the whole northwestern area of Wolji Pond was used as farm land areas. Second, they might be 'a drainage facility for controlling the water level in creating Wolji Pond', given that the drainage gate was annexed to the lower shore forming the waterline of Wolji Pond, and that the hight of drainage holes on top of the drainage gate was similar to the full water level of Wolji Pond. Considering the related grounds and circumstance, the latter possibility is high.

Study on Deriving the Items related to Investigation and Planning for the Comprehensive Maintenance Plan of Scenic Sites (명승 종합정비계획 수립을 위한 조사 및 계획 항목의 도출에 관한 연구)

  • Lee, Jae-Yong
    • Korean Journal of Heritage: History & Science
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    • v.51 no.3
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    • pp.88-103
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    • 2018
  • The purpose of this study was to excavate the items of the comprehensive maintenance plan for scenic sites considering sustainability and analyze the needs of them. In this sense, based on 35 reports of the comprehensive maintenance plan established between 2006, when scenic sites started to be designated, and 2017, the items used in 'investigation field' and 'planning field' were examined, and then a survey regarding the needs of the items was conducted using a "5-point Likert Scale", targeting officials at 60 local governments in the whole country. Of 60 local governments, opinions from 48 officials at 45 local governments were analyzed. In order to verify the consistency of their opinions, "Reliability Analysis" was conducted, and Cronbach's alpha coefficient was 0.968 and 0.970 for 'investigation field' and 'planning field', respectively, showing high reliability. As a result of the survey, most opinions generally expressed the needs of 6 items of 'investigation field' of the comprehensive maintenance plan. Especially, the needs to investigate 'historical environment', 'natural environment', 'humanistic environment', and 'landscape' turned out to be high. In addition, as for 'general environment' and 'users', the needs of specific items such as 'distribution of main cultural properties and historic sites' (4.04) and 'acceptance of opinions from local residents and interested parties' (4.15) were found to be high. Besides, the items of 'planning field' also turned out to be needed in general (4.0). Particularly, the needs of 'enhancement of designated value and status' (4.26) and 'the comprehensive maintenance plan for designated areas of cultural properties and historic and cultural environment preservation areas' (4.25) in 'historical environment', 'maintenance of historic buildings at scenic sites' (4.28) in 'humanistic environment', and 'landscape trail planning' (4.28) in 'landscape' were found to be high. In conclusion, the practical items related to investigation and planning of the comprehensive maintenance plan for scenic sites are expected to contribute to effective conservation and management of scenic sites in the future.