• Journal of Sericultural and Entomological Science
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• v.38 no.1
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• pp.13-18
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• 1996

To secure the genetic resources of silkworm, Bomyx mori, the cDNA library was constructed with mRNA isolated from fifth instar larvae. Titer of the cDNA library was about 1.3 X 106 plaques in total. We presumed that the titer covered all transcripts existed in Bombyx mori. Meanwhile, it is knowen that partial cDNA sequences, Expressed Sequence Tags(ESTs), have a good value for the discovery of novel genes and the elucidation of their structures. For this purpose, partial cDNA sequencing was carried out from randomly selected cDNA clones in the library. Partial cDNA sequences of 37 clones were determined and an average of 212 nucleotides of sequence can be read from the clone. The ESTs were searched in GenBAnk database and fifteen ESTs showed significant similarities to enlisted sequences. They included the genes of storage protein, heat shock protein, actin, catalase and so forth. We presumed that the 22 unmatched ESTs were novel genes.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.116-121
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• 2004

Panax ginseng discarded and lower than 4th grade is caused by red skin disease showing red color skin in ginseng. This kind of red skin ginseng is found a lot in Panax ginseng rather than Panax quinquefolium, and it is considered that red skin disease might be caused by gene. Therefore, this study was carried out to detect genes resistant to red skin disease using RT-PCR. RNA was extracted from three years old ginseng root of both red skin and normal portion in the same root. After RNA extraction, PCR amplification was performed from cDNA using many random primers. As a result, specific band for red skin was found. It is considered that the gene forming band has possibility to be related with red skin disease, and this gene should be decided if it's related with red skin disease. If that gene is related with red skin disease, it will be used for transformation to foster for resistance to red skin disease as well as for selection marker. Bowever, if it's not related with red skin disease, more primers should be used to find gene related with red skin disease.

• Analytical Science and Technology
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• v.18 no.3
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• pp.257-262
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• 2005

Mitochondrial DNA (mt DNA) sequence analysis has been a useful tool for species identification of animals and human individuals. Two hypervariable regions (HV1 and HV2) in control region of mitochondrial genome were analyzed for human individual identification. In case of animal species identification, several genes on mt DNA such as cytochrome b (cytb), RNAs, cytochrome oxidases (CO) were used. In this study, co-amplification of HV1 and cytb was carried out in order to check the contamination of animal DNA and to verify the human DNA. The primer sets used in PCR were H15997/L16236 for HV1 and H14724/L15149 for cytb. PCR products for HV1 and cytb were 239 bp and 425 bp, respectively. The appearance of two bands on agarose gel implied the DNA came from human, however the single band of cytb gene represented the non-human animal DNA.

• Korean Journal of Microbiology
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• v.37 no.2
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• pp.120-123
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• 2001

Sphingomonas chungbukensis DJ77 is able to use phenanthrene and biphenyl as the sole carbon and energy source. Mitomycin C curing experiment suggested that polyaromatic hydrocarbon (PAH) utilization in strain DJ77 was plasmid-encoded. The plasmid cured strains were failed to grow on the minimal medium sprayed with biphenyl or phenanthrene. This was evident from southern hybridizations using a previously cloned DNA segment as a probe. There were positive signals in the palsmid DNA of the wild-type strain DJ77 and the absence of hybridizations with chromosomal DNA from the plasmid DNA of the wild-type strain DJ77 and the absence of hybridizations with chromosomal DNA from the palsmid-cured mutant strains.

• Journal of Life Science
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• v.14 no.2
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• pp.296-300
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• 2004

We investigated feasibility of using the formalin-fixed and paraffin-embedded tissue to study mitochondrial mutations in the case that fresh or frozen tissue, or blood samples are not available. Four paraffin blocks of muscle biopsies in Korean MELAS (mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes) patients were chosen. Total DNA was extracted from these blocks for PCR/RFLP analysis, and sequencing was performed to study the most common mutation, A to G transition at nucleotide position 3243 underlying MELAS in the mitochondrial tRN $A^{Leu(UUR)}$ gene. We could identify the A to G mutation at nt.3243 in three MELAS patients. Our results show that the mitochondrial genome of our paraffin blocks is presumably in good condition. Our results are in accordance with the previous findings by other investigators that PCR allows molecular genetic analysis of paraffin-embedded tissues stored in most histopathology laboratories.s.

• Journal of Life Science
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• v.21 no.2
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• pp.273-278
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• 2011

Plants which had been classified to the Scrophulariaceae of the Lamiales were recently reclassified. Many of them were moved to the other families of Lamiales according to the DNA sequences of the plastid DNA. Among those, Melampyrum roseum, Phtheirospermum japonicum, Pseudolysimachion undulata, Lindernia crustacea and Mazus pumilus were chosen for phylogenetic analyses. DNA sequences of 18S rRNA gene and ITS1 of those plants were determined and deposited into GenBank (accession numbers GU359046, GU359047, GU359048, GU359049, GU359050, respectively). Analyses of those DNA sequences confirmed the current classification done on the basis of the plastid DNA sequences of Melampyrum roseum, Phtheirospermum japonicum and Pseudolysimachion undulata. However, it was not possible to classify Mazus pumilus and Lindernia crustacea due to discrepancies of analyses data.

• Journal of Engineering Education Research
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• v.21 no.6
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• pp.54-62
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• 2018

This study is an analysis of the process of the discovery of the DNA double helix structure from an engineering literacy education perspective. The explanation of the DNA double helix structure by James Watson and Francis Crick in 1952 is a well-known scientific episode. The process is also a combination of various incidents that can frequently happen in competitive engineering research and development situations. Therefore, the process of the discovery of the DNA structure is a remarkable event that can cover all subjects, such as engineering and ethics, research ethics, communication between researchers, engineering and leadership, engineering and teamwork, and engineering and women. This paper focuses on analyzing the research ethics issues associated with Rosalind Franklin and comparing and analyzing the three teams that were very close to the discovery of the DNA structure. By looking at why the Watson and Crick team got the final answer instead of the Linus Pauling's team or the Maurice Wilkins and Rosalind Franklin's team, the virtues of the technology development process that should be taught in engineering literacy education will be naturally presented.

• Proceedings of the Korean Information Science Society Conference
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• 1999.10b
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• pp.12-14
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• 1999

Promoter는 transcript start site 앞부분에 위치하여 RNA polymerase가 높은 친화성을 보이며 바인당하는 DNA상의 특별한 부위로서 여기서부터 DNA transcription이 시작된다. function이나 tissue-specific gene들의 그룹별로 그 promoter들의 특이한 패턴들의 조합을 발견함으로써 Specific한 transcription을 조절하는 것으로 알려져 있어 promoter로 인한 그 gene의 정보를 어느 정도 알 수가 있다. 사람의 housekeeping gene promoter들을 EPD(eukaryotic promoter database)와 EMBL nucleic acid sequence database로부터 수집하여 이것들 간에 의미 있게 나타나는 모든 패턴들을 optimization algorithm으로 알려진 genetic algorithm을 이용해서 찾아보았다.

• The Science & Technology
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• v.19 no.2 s.201
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• pp.79-84
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• 1986

- 환경내의 유전물질 빈도를 모니터 하기 위한 DNA 탐지장치 - RRV1 및 RRV2 로보트 - 기능적 특성을 예견하기 위한 비천연 단백질의 설계와 합성 - 고분해능의 주사 이온 미세탐침 - 에크타켐 DT60 혈액분석기 - 뇌일혈 치료시스템 - 레일건 - 소프트스트립 - 고수준 핵폐기물 저장용 납-철-인산염 유리공정 - 단백질과 DNA의 합성 및 분석 - 물의 광전해용 전극 - 애버트 비루스용 고급휴지 - 3480 자기테이프 서브시스템

• Proceedings of the Korean Information Science Society Conference
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• 2008.06c
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• pp.64-67
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• 2008

DNA Chip을 이용한 실험은 그 결과에 대하여 대용량의 정보를 쏟아내고 있다. 이러한 데이터를 분석하는 다양한 기법 중, 미리 정해진 클래스에 데이터를 해당하는 클래스로 분류하는 기법인 분류화를 수행하여 의도한 목표를 위한 규칙을 찾아내고자 한다. 본 논문에서는 이를 위해 DNA Chip과 같은 방대한 양의 정보 분석에 대하여 적합한 생태계 모방 알고리즘인 PSO Algorithm을 사용하여 분류 규칙을 발견하여 이를 데이터에 적용, 분류하는 연구를 기술하고 있다.