• Journal of Embryo Transfer
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• v.19 no.3
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• pp.283-289
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• 2004

This study was conducted to determine the effects of incubation time after cooling on mouse meiotic spindle and chromosome alignment and the optimal incubation time for their restoration. Oocytes at the metaphase II were obtained from superovulated mice. Control oocytes were held at 37$^{\circ}C$ during the experiment. Oocytes were rapidly cooled to $0^{\circ}C$, held for 30 minutes, warmed and incubated at 37$^{\circ}C$ for 5, 15, 30, 60 and 120 minutes, respectively. The morphological features of spindle and chromosomes in oocytes were evaluated by immunofluorescent staining. Meiotic spindle of control oocytes exhibited a normal-looking bipolar configuration(barrel-shaped) and highly fluorescent microtubles. The chromosomes were clustered in a discrete bundles at metaphase plate. Disassembly of meiotic spindle and chromosome dispersion were occurred immediately after chilling of oocyte. Fluorescence intensity index(FIS), normal chromosomes aligned and normal spindle configuration were compared according to incubation time at 37$^{\circ}C$. Restoration of a barrel-shaped spindle and normal chromosome alignment was occurring after 5 minutes incubation at 37$^{\circ}C$, improved as a incubation time increased, and decreased gradually after 120 minutes incubation(P<0.05). The optimal incubation time for restoration of meiotic spindle and chromosomes in cooled oocytes was 60 minutes.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2011.06a
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• pp.795-796
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• 2011

• Journal of Plant Biology
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• v.38 no.1
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• pp.115-119
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• 1995

In order to clarify the relationship between the activity and age of vacular cambium in the rhizome of Botrychium ternatum, the cambia of the 6th and 10th internodes from its shoot apex were compared ultrastructurally and histochemically. The cambium in the 6th internode had two to four radially arranged cells in each row, and the cells are radially flattened and had thiner tangential walls than radial. The cambial cells were ultrastructurally characterlized by the presence of a large vacuole, few storage materials such as starch grains and lipid droplets, and various cell organelles such as mitochondria, endoplasmic reticulums and dictyosomes in cytoplasm. On the other hand, the cells of cambium in the 10th internode showed irregular arangement and somewhat enlargement in radial plane. The cells were filled with numerous storage materials and small vacuoles and had few cell organelles except for mitochondria. In addition, the activity of acid phosphatase appeared in the cambium of the 6th internode but of the 10th. From the above results, it is clear that the vascular cambium of the 6th internode was active and that of the 10th was dormant. Thus we reached a conclusion that the rhizome of '||'&'||'trychium ternatum has determinate cambium.

• Proceedings of the Acoustical Society of Korea Conference
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• autumn
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• pp.317-322
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• 2001

정보 통신 기술의 발달이 인간 생활에 많은 편리함을 가져다 주었지만 개인의 정보 유출이나 소음 공해 등의 여러 가지 문제점도 야기 시켰다. 특히 휴대 전화 통화시 사용자의 개인적인 정보들이 공공 장소에서 그대로 노출이 되고, 또 그러한 통화로 인해서 타인에게 소음 피해를 주기도 한다. 이러한 병폐를 막기 위해서 휴대 전화 사용자는 최대한 작은 소리로 통화를 하되, 상대방에게 의도하고자 하는 바를 확실히 전달하여야 한다. 그러기 위해서는 휴대 전화에 근접 통화가 가능해야 하며, 일정한 방향의 방향성을 가져서 주위의 잡음에 대해 독립적으로 작동하도록 해야한다. 본 논문에서는 이러한 기능을 향상시키기 위하여 음향학적 이론을 바탕으로 휴대전화에 사용되어지는 마이크로폰을 새로 설계, 제작하고 그 성능을 시험하였다. 아주 작은 소리로 근접 통화를 할 때 발생하는 팝핑(popping) 현상이 최소한이 되도록 미세관 배열을 적용하였으며, 외부 소음 영향을 줄이기 위하여 방향성을 증폭시키고자 음원의 위치에 초점을 맞추는 기술을 적용하였다. 방향성 증폭은 4개의 마이크로폰 사용과 근거리 음원의 방향성 증가를 위한 신호 처리기술을 적용하였다. 이 기술의 적용 효과를 실험을 통하여 검증할 수 있었다.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2001.11b
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• pp.1335-1340
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• 2001

The information technology brought us many kinds of conveniences to our life, but it also caused social problems such as privacy interference, unexpected personal information leaks, and nose generation by telephonic talks, etc. In this paper, the microphone system of the cellular phone is developed to prevent these problems caused by progress of information technology. The developed system was designed to detect only acoustic signals from a human being in the presence of various kinds of background noises. A windscreen was designed by use of micro-channels to eliminate the popping noise by the wind from the mouth of a speaker and four microphone array and signal processing techniques are applied to reduce background noise. The impact of the developed system was evaluated by experimental tests. The results show that the system can improve the required functions considerably.

• Development and Reproduction
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• v.2 no.1
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• pp.75-80
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• 1998

메기 정자는 그 길이가 약 62.5 \mu m이며 구형의 핵, 짧은 중편 및 꼬리를 ㄱ진 일반적인 메기류 정자의 미세구조적 특징을 나타내었다. 정자는 대부분의 경골어류의 정자에서와 같이 첨체를 가지고 있지 않았으며 염색질은 완전치 농축되어 있었다. 핵와(nuclear fossa)는 약 0.6 \mu m 함입되어 있었고 그 속에 기부 중심립과 말단 중심립의 일부가 포함되어 있었다. 두 중심립은 140 \circ C의 각도로 배열되어 있었으며 말단 중심립에서 9개의 부수체가 언형질막을 향하여 배열되어 있었다. 미토콘드리아는 중편 세포질에서 2층 또는 3층으로 배열되어 있었으며 핵의 후반부와 꼬리의 기부를 둘러싸고 있었다. 꼬리는 축사만으로 구성되어 있었으며 lateral fins는 관찰되지 않았다. 메기 정자의 가장 큰 구조적 특징은 중편 세포질에 구성되어 있는 관구조(tubular structure)이었다. 대부분의 경골어류의 정자는 중편 세포질에 미토콘드리아만을 포함하고 있으나, 메기 정자에서는 중편 세포질의 전반부에 미토콘드리아가 포함되어 있고, 후반부에는 소관이 모여 망상구조를 형성하는 관구조가 잘 발달되어 있었다. 이와 같은 관구조는 현재까지 Characiformes의 정자 이외의 다른 경골어류에서는 보고된 바 없으며 이러한 구조는Characiformes과 메기류의 계통학적 관계를 연구하는데 매우 중요한 형질로 여겨진다. ^u The spermatozoa of Silurus asotus are appoximately 62.5 \mu m in length and relatively simple cells composed of spherical head, a short midpiece and a tail as in most Siluriformes. The ultrastructure of the spermatozoa of S. asotus is characterized by the following features. The nucleus measuring about 1.5 \mu m in length is depressed with a deep nuclear fossa of about 0.6 \mu m in length, two fifth of the nuclear diameter. The fossa contains the proximal centriole and the half of the distal centriole. Two centrioles form an angle of approximately 140 \circ to each other. the nine satellite rays radiate from the outer surface of the distal centriole. the mitochondrea surround the basal nucleus and the axoneme, and are arranged in two or three layers in the postnuclear cytoplasm. The lateral fins are lost in the sperm tail. The most significant feature is manifested in the midpiece. The midpece comprises two parts, the mitochondria and the tubular structure unlike other teleost fishes containing only the mitochondria. The tubular structure was reported only in the spermatozoa of Citharinus belonging to the characiformes of teleost fishes. Thus it is considered to be a good characteristics for the study of phylogenetic link between Siluriformes and Characiformes.

• Korean Journal of Ichthyology
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• v.31 no.1
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• pp.1-6
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• 2019

The spermatozoa of Hemiculter eigenmanni is similar to other cyprinid by spherical head containing a nucleus with highly condensed chromatin, a short midpiece with mitochondria and a flagellum located tangentially to the head. The fine structure of cyprinid spermatozoa described classical characteristics of Cyprinidae spermatozoon comprising the absent of acrosome, the shallow nucleal fossa, postnuclear distribution of mitochondria and the lateral insertion of flagellum. However there were some structural differences for their morphology, in the mitochondria and the orientation of centrioles. The proxomal and distal centrioles are oriented approximately $145^{\circ}$ to each other. The mitochondria of 8 or 10 in number are arranged in two or three layers.

• Applied Microscopy
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• v.38 no.2
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• pp.97-105
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• 2008

The fine structural modification of the granulocytes between the molt and intermolt period were investigated by the transmission electron microscopy. The granular hemocytes of the spider Araneus ventricosus were composed of three subtypes: eosinophilic granulocytes (EGs), basophilic granulocytes (BGs) and cyanocytes. Both of the EGs and BGs have electron dense granules within their cytoplasms, however the granules of BGs are larger than those of EGs. During the molt period, some of the EGs have fine structural modification in their cell organelles including formation of phagosomes as a result of active phagocytosis. However, the BGs have no phagosomes, but electron densities of the granules are changed to lower states than the intermolt period. The cyanocyte is the biggest hemocyte among the granulocytes. They contain numerous hemocyanin crystals in the cytoplasm with some electron-lucent vacuoles. During the molt period, some of the cyanocytes are changed to irregular shapes. High magnification electron micrographs reveal that the lattice sub-structure of the hemocyanin crystals are very similar to those of microtubules, and each tubule is composed of approximately 20 filaments with fine fibrillar structure.

• Korean Journal of Soil Science and Fertilizer
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• v.7 no.2
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• pp.65-70
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• 1974

Micromorphological features of Bhorizon of Honam series were investigated by means of thin sections of natural undisturbed, oriented soil samples. Thin sections show that the B horizons have argillic accumulations. Argillic horizon was developed at the utmost in B21t horizon. Channel, and skew plane cutans were identified in B21t horizon and stress cutans are presented in B24t horizon. Such a continuous oriented argillans related to channels and skew planes decreased with increasing depth.

• Journal of Marine Life Science
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• v.8 no.2
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• pp.178-185
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• 2023

This study was described the microscopic anatomy of male reproductive organs and spermatophore necessary for understanding the reproductive ecology of the long arm octopus Octopus minor. The long arm octopus was a species that has sexual dimorphism that can distinguish between sex based on the presence of hectocotylus. Male reproductive organs consisted of testis, primary spermatic duct, spermatic gland, secondary spermatic duct, spermatophoric gland and spermatophoric sac. Histologically, the testis was testicular tubule type and male germ cells showed a layered arrangement. The primary spermatic duct was a tube connecting the testis and spermatic gland, and consisted with epithelial layer and connective tissue. The spermatic gland was located between the primary and secondary spermatic duct, and the epithelial layer was composed of epithelial cells and mucous cells. Mucous cells reacted blue in the AB-PAS (pH 2.5) reaction and purple in the AF-AB (pH 2.5) reaction. The secondary spermatic duct was a short tube connecting spermatic gland and spermatophoric gland, and folds were developed in lumen. The spermatophoric gland consisted of numerous tubular glands and secretory cells had eosinophilic granules. The spermatophoric sac was shape of pouch, folds were developed in lumen, and vacuolar secretory cells were present in the epithelial layer. The spermatophore was 83.5 mm long and consisted of cap thread in anterior portion, ejaculatory apparatus and cement body in medial portion, sperm mass in posterior portion.