• Title/Summary/Keyword: 미생물 정화

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Characterzation of a Cadmium-ion Tolerant Strain of Hansenula anomala (카드뮴 내성 Hansenula anomala 균주의 특성)

  • 유대식;송형익;정기택
    • Korean Journal of Microbiology
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    • v.24 no.1
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    • pp.57-61
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    • 1986
  • In order to remove of cadmium from waste water an identification of a cadmium-ion tolerant yeast B-7 isolated from the sludge of zinc mining district was studied. By the taxonomecal characteristics of strain B-7 it was identified as Hansenula anomala B-7 or similar strain. The cadmium-ion tolerance of the strain B-7 was determined as $2,700{\mu}g/ml$ of cadmium-ion by density gradient agar plate method. The strain B-7 grew well in an aqueous medium containing $1,000{\mu}g/ml$ of cadmium-ion.

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Theoretical Estimation of Stoichiometry for Biodegradation of Hazardous Organic Compounds (유해유기물질에 대한 미생물 분해 반응식의 이론적 예측)

  • 우승한;박종문
    • Journal of Soil and Groundwater Environment
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    • v.8 no.2
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    • pp.70-77
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    • 2003
  • Theoretical estimation of overall stoichiometry for the microbial degradation of hazardous organic compounds is described. Half-reaction method based on microbial energetics was used in the theoretical estimation. In addition to the half-reaction method, other theoretical methods such as intermediate formation, oxygenation reaction, and estimation of the standard free energy of formation by group contribution theory were also applied. As a case study, the application of these methods was demonstrated for the estimation of microbial kinetics in the biodegradation of phenanthrene which was chosen as a model hazardous organic compound along with glucose and hexadecane. The cell yield, oxygen requirement, nitrogen requirement, and mineralization ratio could be estimated from the overall stoichiometry. It is believed that these theoretical estimation methods are useful tools for practical design and assessment of bioremediation of soil and groundwater contaminated with hazardous organic compounds.

Development and Application of an In Situ Technology to Treat Various Soil and Groundwater Contaminants

  • Goltz, Mark N.
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.10a
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    • pp.89-110
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    • 2003
  • The limitations of conventional soil and groundwater contamination remediation technologies have motivated a search for innovative technologies; particularly in situ technologies that do not require extraction of contaminants from the subsurface. All engineered in situ remediation systems require that the contaminant be mixed with a remedial compound. Horizontal flow treatment wells (HFTWs), an innovative technology that consists of a pair of dual-screened treatment wells, were used at a trichloroethylene (TCE) contaminated site to efficiently achieve this mixing of contaminant and remedial compound in order to effect in situ bioremediation (McCarty et al., 1998). In this paper, the potential of HFTWs to treat chlorinated aliphatic hydrocarbons (CAHs) as well as other soil and groundwater contaminants of concern, such as nitroaromatic compounds (NACs), perchlorate, and methyl-tert-butyl ether (MTBE), is examined. Through a combination of laboratory studies, model analyses, and field evaluations, the effectiveness of this innovative technology to manage these contaminants is investigated.

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Development of In-Situ Monitoring System for measuring soil gas (토양가스 측정을 위한 현장 모니터링 시스템 개발)

  • Yu, Chan;Lee, Jong-Beom
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 2005.10a
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    • pp.410-416
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    • 2005
  • 생물학적 통풍법은 유류오염 지역에 자주 적용되는 정화공법이다. 이 과정은 지중에 산소를 충분히 공급함으로서 토착 미생물에 의한 오염성분의 분해를 가능하게 한다. 따라서 이 공법의 적용시 공정진행에 따른 공법의 효율성 분석과 장기적인 정화효율 예측을 위한 지중 가스성분의 모니터링 시스템 도입이 매우 중요하다. 그러나 우리나라에서는 아직 그 적용사례가 보고된 바 없다. 따라서 본 연구에서는 현장에서 토양가스 성분의 변화를 모니터링할 수 있는 시스템을 개발하여 적용한 사례를 시스템의 구성과 측정방법, 관측결과를 중심으로 소개하였다. 현장적용 결과는 토양가스 모니터링 시스템은 운용 시작 후 6개월동안 센서나 측정장비에서 문제가 발생되지 않았으며, 공정관리를 위한 공법효율성 분석에 필요한 자료를 지속적으로 제공하고 있다.

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A Case History of the Remediation of a Petroleum Contaminated Site using the Bioventing Method (생물학적 통풍법을 이용한 유류오염지역 복원사례)

  • Yu, Chan;Yun, Sung-Wook;Jung, Yeon-Wook;Park, Han-Eun
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 2005.10a
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    • pp.610-613
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    • 2005
  • 물리 화학적 원리에 의한 환경오염 정화방법은 종종 낮은 효율을 보이면서 공사비 부담은 큰 것으로 나타나고 있다. 그러나 생물학적 방법들은 이러한 단점들을 극복하면서 토양과 지하수를 정화할 수 있는 대안으로서 그 가능성을 입증하고 있는 실정이다. 그 중에서 생물학적 통풍법은 유류오염지역에 적용하고 있으며 그 원리는 지중 오염지역에 충분한 산소를 공급함으로서 오염성분이 토착 미생물에 의해 제거되도록 하는 것이다. 본 발표는 유류오염 지역에 적용된 생물학적 통풍법의 효율을 평가하여 제시하였다.

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Soil Health Assessment of Soil Washing and Landfarming Treated Soils (토양세척 및 토양경작 정화 토양의 건강성 평가)

  • Yong min Yi;Kijune Sung
    • Journal of Environmental Impact Assessment
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    • v.32 no.2
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    • pp.112-122
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    • 2023
  • To restore the ecological function of contaminated soil and maximize the ecological services provided by the soil, besides the toxicity orrisk caused by pollutants, the functional aspects of the soil ecosystem should be considered. In this study, a method for evaluating the health of cleaned soil was presented, and the applicability of the proposed evaluation method was examined by applying it to soil treated with washing and landfarming. Productivity, habitat, water retention capacity, nutrient cycling, carbon retention capacity, and buffering capacity were used as soil health evaluation indicators. The results showed that the soil health was not completely recovered after remediation, and even in the case of the washed soil, the health was lower than before remediation. On the other hand, there was no significant change in soil quality due to oil pollution, but soil health deteriorated. Unlike the slightly improved soil quality after landfarming treatment, soil health was not completely restored. Therefore, the results of this study indicate that it is desirable to consider both soil quality and health when evaluating the remediation effect. The soil health evaluation method proposed in this study can be usefully utilized for the sustainable use of cleaned soil and to promote ecosystem services.

Treatment of Waste Food using Mixed Microorganisms Responsible for the Degradation of Malodor Compounds (혼합 미생물에 의한 음식쓰레기 처리와 악취 제거)

  • Yun, Soon-Il
    • Microbiology and Biotechnology Letters
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    • v.31 no.4
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    • pp.413-420
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    • 2003
  • To explore the effective treatment methods for waste food generating malodor compounds, mixed microorganisms (MM) capable of deodorizing of domestic animal waste were applied using household composting box. The mixture of 5 kg whole chips as a bulk agent, 2 kg MM and 1 kg of waste food were input into the compost reactor and agitated. Waste food was supplemented every 24 hours. As the results, the composting volume was stable at 13∼14 L for 10 days. In the initial compost process with MM, the pH and temperature were increased more quickly than that of without MM. Also, the conductivity recognized as a barometer of compost was increased from 0.2 to 2.4 mS/cm that was higher than 1.3 mS/cm of without MM, for 10 days. The malodor compounds generated from waste food treatment such as sulfur compounds and volatile fatty acids were effectively reduced about 90∼l00%, and 70∼80% for 8 days, respectively. The microorganisms growing under the condition of alkaline phase and higher temperature were dominated during the compost Moreover, it was demonstrated that inoculated Bacillus cereus HY15 dominated during the compost results in responsible to the effective treatment of waste food.

Draft genome sequence of Zhongshania marina DSW25-10T isolated from seawater (해수에서 분리된 Zhongshania marina DSW25-10T 의 유전체 서열분석)

  • Oh, Ji-Sung;Roh, Dong-Hyun
    • Korean Journal of Microbiology
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    • v.54 no.4
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    • pp.480-482
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    • 2018
  • The draft genome sequencing for Zhongshania marina $DSW25-10^T$, isolated from deep seawater of East Sea in Korea, was performed using Illumina HiSeq platform. As a result, the draft genome was comprised of a total length of approximately 4.08 Mbp with G + C content of 49.0%, and included a total of 3,702 protein-coding genes, 3 rRNA genes, 39 tRNA genes, 4 non-coding RNA genes, and 36 pseudogenes. In addition, the metabolic pathways of aliphatic and aromatic compounds were identified. In light of these metabolic pathways, Zhongshania marina $DSW25-10^T$ is expected to be a useful bioremediation resource.

Nutrient Removal Efficiencies in Marsh- and Pond- type wetland Microcosms (실험실 조건에서 소택지, 연못형 습지의 영양염류 제거효율 평가)

  • Song, Keunyea;Kang, Hojeon0g
    • Journal of Wetlands Research
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    • v.7 no.4
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    • pp.43-50
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    • 2005
  • Wetlands can transform or remove pollutants from water body, such as nitrogen, phosphate, and organics. Many researches were conducted in relation to uptake process by aquatic plants in wetlands. However, water purification processes in wetlands are the results of physical, chemical and biological, especially microbiological reactions. As such, understanding on microbial processes is of great importance. In this study, we used pondor marsh-type wetland microcosms for investigating the water purification capacity and microbial functions, namely, extracellular enzyme activities, nitrification and denitrification. In a pond system, removal efficiencies of $NO_3{^-}$ and $PO{_4}^{3-}$ were 96% and 100 % respectively, while those in a marsh system were 94%, 100% respectively. These high removal efficiencies appeared to be caused by high adsorption ability to soils and microbial functions in wetland.

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