• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.3
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• pp.457-463
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• 2013

The purpose of this study was to apply the Hazard Analysis Critical Control Point (HACCP) system to the production of dressing. The hazard analysis examined the main materials, industrial water, microbial evaluation, and airborne microorganisms of each working area, as well as the pathogenic microbial contamination risk. The survey was conducted at SJ Company in Jincheon (Chungchengbuk-do), Korea for 30 days from April 1, 2012 to April 30, 2012. The results showed that raw material microorganisms had a total plate count in industrial water below $3.00{\times}10$ CFU/mL in working room I, working room II, the packing room, washing water, and the inspection room for five times in each place. During dressing production (including heat treatment and mixing), general bacteria were detected at an average of $3{\times}10$ CFU/mL, but yeast, mold, and pathogenic bacteria were not detected. Airborne microbiological evaluation (for total plate count, yeast, and mold) found levels below the legal limit at each working area. While workers were positive for microbes in total plate counts, coliform and Staphylococcus aureus were not detected. In conclusion, standards for hygienic management should be established to prevent and decrease hazards, such as general bacteria and pathogenic microorganisms (for example, E. coli, B. cereus, Listeria spp, Salmonella spp, Staph. aureus, Clostridium perfringens, yeast, and mold), and to found critical limits for microorganisms with an HACCP system.

• Food Science and Preservation
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• v.12 no.1
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• pp.8-16
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• 2005

To develop natural antimicrobial agents for extending the self-life of Kimchi, the effect of botanical antimicrobial agent-citrus products(BAAC) on microorganisms related to Kimchi spoilage was investigated. The inhibitory effect of BAAC on microorganisms related to Kimchi spoilage was increased according to the concentration of BAAC. Antimicrobial activities of BAAC against microoiganisms related to Kimchi spoilage were remarkably high. The effect of BAAC on the cellular membrane function of microorganisms showed the perturbation of cells in the presence of BAAC. Direct isualization of microbial cells by using both transmission md scanning electron microscope showed microbial cell membrane was destroyed by treating with BAAC. It could be confirmed that BAAC completely inhibit the growth of the test strains. The pH of BAAC-added Kimchi was a little higher than that of the control through the fermentation period. Titratable acidify, vitamin C and viable cells in BAAC-added Kimchi were changed more slowly than those in the control. Sensory evaluation did not show any significant difference between $0.01\%$ BAAC-added Kimchi and the control that showed the best palatabilities during fermentation.

• Korean Journal of Clinical Laboratory Science
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• v.54 no.4
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• pp.239-248
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• 2022

The taxonomy of bacteria in the field of clinical microbiology is in a state of constant flux. A large-scale revamping of the classification and nomenclature of anaerobic bacteria has taken place over the past few decades, mainly due to advances in molecular techniques such as 16S rRNA and whole genome sequencing (WGS). New genera and species have been added, and existing genera and species have been reclassified or renamed. A major role of the clinical microbiological laboratories (CMLs) is the accurate identification (ID) and appropriate antimicrobial susceptibility testing (AST) for clinically important bacteria, and rapid reporting and communication of the same to the clinician. Taxonomic changes in anaerobic bacteria could potentially affect the choice of appropriate antimicrobial agents and the antimicrobial breakpoints to use. Furthermore, current taxonomy is important to prevent treatment failures of emerging pathogenic anaerobes with antimicrobial resistance. Therefore, CMLs should periodically update themselves on the changes in the taxonomy of anaerobic bacteria and suitably inform clinicians of these changes for optimum patient care. This article presents an update on the taxonomy of clinically important anaerobic bacteria, together with the previous names or synonyms. This taxonomy update can help guide antimicrobial therapy for anaerobic bacterial infections and prevent treatment failure and can be a useful tool for both CMLs and clinicians.

• Annals of Clinical Microbiology
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• v.19 no.1
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• pp.20-23
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• 2016

In the RNA-based study, it is important to extract high-quality RNA. However, RNA extraction from Mycobacterium tuberculosis is problematic due to its thick, waxy cell wall rich in mycolic acid, which renders the cells resistant to lysis. Using TRIzol reagent and several powerful bead-beating steps, a high quantity of RNA was obtained.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.160-162
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• 2004

본 연구는 도계장의 HACCP 적용에 따른 자료 확보를 위하여 도계공정 단계별 및 닭고기의 저장중의 미생물 증감 추이를 조사하였다. 도계장 규모별로 대규모 2곳과 소규모 2곳을 선정하여 여름, 가을, 겨울, 봄에 걸쳐 각 도계장마다 2회씩 시료를 채취하여 미생물검사를 도계공정 단계별로 실시한 결과 도계공정 초기인 내장적출 전, 후 단계에서 채취한 시료에서는 $10^5{\sim}10^6CFU/m{\ell}$이었다가 세척, 냉각 단계를 거쳐 냉장 단계에서는 $10^3{\sim}10^4\;CFU/m{\ell}$ 수준으로 균수가 감소하는 것으로 나타났다. 낙하세균수 조사결과는 계절에 관계없이 탕적실과 내장 적출실의 공기오염도는 높은 편이었고, 세척실과 냉각실은 양호하였으며 냉장실에서는 colony가 거의 검출되지 않았다. 닭고기를 $4^{\circ}C$에서 저장하였을 때 미생물 균 수의 변화를 조사한 결과 일반세균수는 저장 초기에 미세한 증가를 보이다가 저장후기(6${\sim}$12일) 급격한 증가를 보여 주었고, 조사한 미생물 중 내냉성균수가 가장 높은 균 수의 증가를 나타내었다. 대장균, 대장균군은 저장 초기 $10^1\;CFU/m{\ell}$ 수준이다가 저장 6일 이후로는 검출되지 않았다. 효모와 곰팡이 균은 $4^{\circ}C$ 저장기간 중 균수가 꾸준히 증가하는 경향을 보여 주었다.

• Applied Biological Chemistry
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• v.48 no.2
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• pp.120-127
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• 2005

Electron beam irradiation was applied to examine the microbial growth and qualities of vacuum-packaged chicken breasts. Chicken breast samples were irradiated at dose of 2, 4, 8, 12, and 16 kGy, respectively. After irradiation, chicken breast samples were individually vacuum-packaged and stored at $4^{\circ}C$. Microbiological change of irradiated vacuum-packaged chicken breasts showed that populations of total bacteria, yeast and mold, total coliform, and salmonella spp. in chicken breasts were significantly reduced with increasing irradiation dose. The pH values of vacuum-packaged chicken breasts were not significantly changed among treatments. Lipid oxidation measurements showed that TBARS values of vacuum-packaged chicken breasts increased with increase of irradiation dose, and gradually increased during storage. Color measurements showed that irradiation reduced Hunter a value of vacuum-packaged chicken breasts with increasing irradiation dose. However, Hunter L and b values of vacuum-packaged chicken breasts were not significantly altered among treatments. Sensory quality results of vacuum-packaged chicken breasts showed that there were no significant changes among the samples irradiated. These results indicate that irradiation can be used to improve the microbial safety and qualities of poultry products.

• Monthly Korean Chicken
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• v.4 no.7 s.37
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• pp.2-6
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• 1998

2003년 모든 축산물작업장 HACCP 시행 - 농림부, 육류에 대한 미생물 검사 강화 - 전국 축사시설 7월중 일제조사 - 농림부, 축산물등의 표시에 관한 기준 고시 - 필리핀, 닭고기의 공급감소로 가격 상승 - 축산관련단체협의회 활성화 - 1인당 닭고기 소비량 6,07Kg으로 $3{\%}$감소 - 인도 육류소비 닭고기가 주도 - 캐나다, 97년 1인당 닭고기 25.6Kg소비 - 소비부진으로 닭고기 복철 특수 기대 어려워 - 사육농가 유류대, 연동 적용키로

• Microbiology and Biotechnology Letters
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• v.6 no.4
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• pp.181-185
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• 1978

The competitions were made between two kinds of Kochujang, one is the native fermented Kochujang which is fermented at 20$\pm$2$^{\circ}C$, and the other is quick fermented Kochujang which is maintained at 35$\pm$2$^{\circ}C$ for 30 days. The results were showed that the general compositions, amino acid, nitrogen and flavor of quick fermented Kochujang were superior to the native fermented Kochujang.

• Microbiology and Biotechnology Letters
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• v.14 no.2
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• pp.205-208
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• 1986

The 23 serovars of Bacillus thuringiensis strain were commonly gram-positive and motile, formed endotoxin crystals, produced acid and alkali in the KIA media, and acid from glucose, hydrolyzed starch, and reduced nitrate but did not produce H$_2$S, oxidase and indole, did not decompose lysine, ornithine, phenylalanine, malonate, lactose, dulcitol, adonitol, inositol, sorbitol, arabinose, raffinose, rhamnose, maltose, and xylose. Eighteen serovars were positive in the MR tests and 15 in the VP tests. Four serovars used citrate. Five serovars produced urease, 5 $CO_2$ from glucose, 2 DNase, and 15 lecithinase. Twelve serovars decomposed arginine, 11 did sucrose, 2 manitol, and 9 salicin Serovar tohokuensis did not hemolyze, but the others did.

• Korean journal of food and cookery science
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• v.25 no.3
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• pp.350-356
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• 2009

In order to develop a natural food preservative, UlGeum (Curcuma longa L.) powder was extracted with ethanol, and its antimicrobial activity was investigated. This ethanol extract showed antimicrobial activity against Bacillussubtilis, Escherichiacoli, and Staphylococcus aureus, and the resulting inhibition zones against the microorganisms by the extract (4 mg/disc) were 11, 10, and 8.5 mm, respectively. To test for further food preservation effects, solutions of the extract were added to rice cakes and noodles at concentrations of 0%, 0.25%, 0.50%, 0.75%, and 1.00%, respectively. Microbial growth was less in the UlGeum ethanol extract-added groups than in the control group. In sensory evaluations, there were significant differences among the groups of rice cakes and noodles in terms of color, taste, flavor, chewiness, and overall acceptability (p<0.05), and the 0.5% added groups received significantly higher scores than the other groups. Furthermore, as storage time increased, the sensory scores of the rice cakes and noodles significantly decreased in all groups (p<0.05). These results suggest that UlGeum is effective for increasing the shelf-life of rice cakes and noodles.