• The Microorganisms and Industry
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• v.16 no.1
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• pp.28-39
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• 1990

Biosensor는 모든 종류에서 공히 생체물질로 이루어지는 감지 부분, transducer 그리고 전자부분으로 이루어진다. 따라서 Biosensor는 사용하는 생체물질, transducer로 그리고 측정되는 물질에 따라 분류된다. Biosensor에 이용될 수 있는 생물반응은 pH 변화, ion 생산및 소비, 기체생산및 소비, 발열, 발광, 전자전달, 질량변화 등을 들 수 있다. 이상의 반응을 유발하는 생체물질은 효소, 항체등 단백질과 세포, 조직등 다양하다. 여기에서는 transducer의 특징을 먼저 살펴보고 biosensor 각론에서 이들 생체물질의 반응 특징을 소개한다.

• Bulletin of Korea Environmental Preservation Association
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• s.405
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• pp.17-21
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• 2013

범세계적으로 강화되고 있는 화학물질 및 유해화학물질 함유제품 규제에 대한 기업의 체계적이고 효율적 대응 대책 마련이 시급하다. 이률 위해서는 우선적으로 제품에 함유된 규제 물질을 확인하고 제조공정 유입 및 사용을 차단하기 위한 공급망내 물질정보 생산 관리 전달을 통합 관리하는 사업장 대응시스템 구축이 필요하다.

• Korean Chemical Engineering Research
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• v.58 no.3
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• pp.450-457
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• 2020

In the previous study, there was a big difference between the tendency of the delivery efficiency of Yo-Pro-1 and the expression efficiency of the CFP gene, but there was a problem that could not provide a clue to this problem. Therefore, this study aimed to present a clue to this problem by quantifying and comparing the delivery efficiency after labeling DNA using a fluorescent dye, which was one of the methods for quantifying biomolecules. As a fluorescent dye for labeling, Yo-Pro-1 was used, and the delivery efficiency of the fluorescent dye Yo-Pro-1 and the labeled DNA was compared. The delivery efficiency of Yo-Pro-1 and labeled DNA according to the voltage condition of the digital electroporation system was maximized at 96 V, and the delivery efficiency tended to decrease as the voltage increased further. In the comparison of the delivery efficiency of Yo-Pro-1 and labeled DNA according to the number of voltage application conditions, the delivery efficiency was maximized at the number of 8 voltage application times for both delivery materials, and the delivery efficiency tended to decrease as the number of voltage application increases further. Through the two results, it was confirmed that the delivery efficiency using Yo-Pro-1 in the digital electroporation system represents the delivery efficiency of the system well. In addition, through the results of this study, the difference between the tendency of the delivery efficiency of Yo-Pro-1 and the expression efficiency of the CFP gene shown in the preceding study was not the result of the difference in the delivery efficiency of the delivery material, but it can be predicted to be due to a problem with the expression process of the genetic material that had been delivered.

• Journal of Life Science
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• v.28 no.5
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• pp.597-604
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• 2018

Although the core mechanisms of Attention Deficit/Hyperactivity Disorder (ADHD) are unknown, several ADHD-associated proteins have been studied. G-protein - coupled receptor kinase interacting protein-1 (GIT1) is a multifunctional adapter protein that affects neuron growth and dendrite formation. GIT1-deficient mice have shown ADHD-like behavior and also recovered through amphetamine treatment. In this study, gliotransmitters were investigated in both intracellular and extracellular space from GIT1-deficient mice. To measure the amount of gliotransmitters, primary astrocyte cultures were taken from the cerebral and cerebellar cortices of wild (WT), hetero (HE), and knock-out (KO) mice. Major gliotransmitters were analyzed using high-performance liquid chromatography. It was observed that the amount of excitatory and inhibitory gliotransmitters were dependent on genotype and showed a change in excitation/inhibition ratios. Interestingly, the major excitatory gliotransmitter, glutamate, existed at the lowest level in WT mice, but the amount of inhibitory gliotransmitters, gamma-aminobutyric acid (GABA) and glycine, varied depending on brain region. Remarkably, an increased amount of GABA was measured at the intracellular cerebrum in WT mice compared with KO mice. It was presumed that KO mice would secrete more inhibitory gliotransmitters to compensate for GIT1 depletion or else acquire a defect to reuptake-secreted GABA. This may be a possible mechanism for ADHD pathology.

• Transactions of the Korean Society of Mechanical Engineers
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• v.17 no.10
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• pp.2624-2633
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• 1993

Mass transfer experiment by naphthalene sublimation method has great advantages in measurement of local transfer coefficients in the region of a three dimensional flow or for a model of complex geometry, which is considered to be very difficult with conventional heat transfer measurements. Mass transfer data obtained by naphthalene sublimation technique are converted to the heat transfer data through heat/mass transfer analogy. This analogy is valid for a simple or laminar flow, but new insight is needed when applying to a turbulent flow or complex flow such as separation, reattachment and recirculation, The purpose of this research is to investigate how geometries and flow conditions incorporate heat/mass transfer analogy. Mass transfer experiments are performed using naphthalene sublimation technique for a flat plate, a circular cylinder, and rectangular cylinders. And mass transfer data are compared with earlier heat transfer measurements for the same geometries. Usefulness of analogy relation between heat and mass transfer is examined with these results.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.11a
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• pp.34-35
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• 1993

Glycine은 GABA와 함께 뇌에 작용하는 중요한 억제성 신경전달 물질이다. GABA가 대뇌등에서 중요한 역할을 하는 반면, GLYCINE은 연수, 척수, 간뇌에 특히 다량 존재한다. GLYCINE은 GLYCINE RECEPTOR에 작용하여 수용체와 연결된 chloride channel의 conductance를 증가시킴으로써 target세포의 활성을 억제한다. 그러므로, glycine의 신경전달체계에 이상이 오면 spastic mouse등에서 볼수 있는 것처럼 neuromuscular disorder가 유발된다. 신경전달 물질은 presynaptic 세포에 자극이 오면 synaptic cleft로 분비가 된후 presynaptic이나 Postsynaptic 세포에 위치한 수용체에 작용하여 생리 활성을 나타내거나, 분해효소에 의해서 생리활성이 없는 물질로 바뀌든지, presynaptic cell에 위치한 transporter(수송체)에 의해서 presynaptic 세포로 reuptake되서 cycle를 끝낸다. Glycine의 경우는 synaptic cleft로 분비된 후 glycine transporter에 의해서 reuptake된다. 그러므로 glycine transporter의 활성 정도는 sysnapse내의 glycine의 농도를 조절하며 더 나아가 glycine이 glycine receptor에 작용하는 시간에 영향을 줌으로써 target 세포의 활성정도를 결정한다.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.05a
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• pp.67-67
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• 2004

다공성 물질을 건조하거나 습한 환경에 노출시키면 열과 수분이 외부로 전달된다. 열 및 수분 전달로 인한 은도 및 습도의 변화는 물질 체적을 변화시켜 습열 응력을 유발시킨다 즉 다공성 제품의 품질은 외양뿐만 아니라 건조 공정 시의 온도, 수분함유량, 응력, 변형률 등의 공정변수에 크게 영향을 받는다. 최근까지도 다공성 물질의 생산 공정은 다수의 공정변수를 갖는 복합공정이기 때문에 이들의 영향을 정량적으로 평가하는 것은 매우 까다로워 현장 경험에 기초한 기술자의 노하우에 의존해 왔다.(중략)

• Journal of the Society of Cosmetic Scientists of Korea
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• v.26 no.1
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• pp.1-16
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• 2000

Keratinocyte 는 피부 표피충의 대부분을 차지하고 있으며, 세포간 신호전달물질 cytokine을 생성분비함으로써 주위의 다른 피부세포의 활성 및 분화능력을 조절한다. 특히 melanocyte 한 세포는 keratinocyte 30 여 세포와 직접적으로 접촉하고 있으며 활발한 신호 전달을 통하여 피부생리의 동적평형 유지에 기여하고 있다. 본 연구에서는 실제 피부에서와 유사한 조건에서 keratinocyte 의 세포의 배출 신호물질인 cytokine 의 변화를 유도하는 물질이 melanocyte 의 피부색소형성과정에 기여하는 바를 평가하기위하여 keratinocyte-melanocyte 의 공동배양계 실험조건과 아울러, UVB 조사에 의해 keratinocyte 로부터 유리되는 물질이 melanocyte를 자극하여 melanin 생합성 유도를 촉진하는 기본 실험 System 을 확립하였고, 가능성있는 친연추출물 원료, 즉, 신이, 강황등의 melanin 생합성 억제능을 본 공동배양계에서 1차 평가한 후 소규모 임상실험에서 미백효능을 최종 평가하였다.

• Polymer(Korea)
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• v.26 no.4
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• pp.543-550
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• 2002

We have synthesized a novel carrier transporting copolymer having triphenylamine moiety as a hole transporting unit and triazine moiety as an electron transporting unit in the polymer side chain. Single-layered organic electroluminescent (EL) devices consisted of ITO/copolymer and emitting materials (DCM, coumarin 6, DPvBi)/Al exhibited maximum external quantum efficiency when the ratio of hole transporting unit and electron transporting unit is 6:4 and the content of emitting material is 30 wt%. Especially, the devices emitted the light of red (620 nm), green (520 nm) and blue (450 nm) corresponding to the emitting materials, respectively. A maximum luminance of ITO/copolymer (6:4) and DCM (30 wt%)/Al EL device was about 500 cd/$m^2$ at a DC drive voltage of 12V.

• Journal of the Korean Electrochemical Society
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• v.2 no.2
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• pp.98-105
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• 1999

In the present study, buoyant force and its stabilizing effects in an electrostatic field were examined systematically in order to reduce the effect of natural convection with thermal stratification in a horizontal fluid-saturated porous layer. The correlation of ionic mass transport induced by double-diffusive convection in a horizontal porous layer has been derived theoretically. And the theoretical model was examined by electrochemical experiments. The theoretical correlation for mass transport which is satisfying Forchheimer's flow equation and based on the micro-turbulence model is derived as a function of soltual Darcy-Rayleigh number, thermal Darcy-Rayleigh number and Lewis number. In the experiment, the mass transport of copper ions in $CuSO_4-H_2SO_4$ solution is measured by electrochemical technique. By assembling theoretical correlation and experimental results, the mass transport correlation induced by double-diffusive convection is proposed as $$Sh=\frac{0.03054(Rs_D-LeRa_D)^{1/2}}{1-3.8788(Rs_D-LeRa_D)^{-1/10}}$$ The present correlation looks flirty reasonable with comparing experimental results, and very promising for the applications of its prototype into various systems involving heat transfer as well as mass transfer, in order to control the effects of natural convection effectively.