• Title/Summary/Keyword: 무지개

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Identification of Differentially Expressed Genes in Improved Rainbow Trout Growth by Treatment with a Fish Myostatin Prodomain Using the Annealing Control Primer System (Annealing control primer system을 이용한 어류 재조합 myostatin prodomain 단백질에 의해 성장이 증가된 무지개송어의 특이적 발현 유전자 탐색)

  • Lee, Sang-Beum;Jin, Hyung-Joo
    • Korean Journal of Ichthyology
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    • v.24 no.2
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    • pp.118-124
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    • 2012
  • The present study was conducted to investigate different gene expression profile between treated poMSTNpro and non-treated in rainbow trout and to identify those genes that are specifically or predominantly expressed in treated poMSTNpro by employing annealing control primer (ACP)-based GeneFishing polymerase chain reaction (PCR). We isolated total RNAs in muscle tissues from the treated poMSTNpro fish by immersion bath technique with fish myostatin prodomain (Paralichthys olivaceus, poMSTNpro) for one month and the other was non-treated poMSTNpro, and synthesized cDNA using annealing control primers (ACP, Seegene, Korea). Using 20 different ACPs for PCR, were cloned sequenced, and analyzed identities of 2 differentially expressed genes (DEGs). According to BLAST analysis, sequences of 2 clones significantly matched database entries and confirmed by semi-quantitative RT-PCR. The functional roles of one up-regulated gene, cytochrome P450 mono-oxygenases 2K1v2 (CYP2K1v2), and one down-regulated gene was Profilin-1 were identified. We identified distinctive gene expression profiles in improved rainbow trout growth by treatment with a fish myostatin prodomain using ACP-based GeneFishing.

Effects of Dietary Soybean Meal in Rainbow Trout (Oncorhynchus mykiss) II. On the Apparent Digestibility, Excretion and Body Composition (무지개송어(Oncorhynchus mykiss) 사료원으로써 대두박의 이용 효과 II. 소화율, 배설 및 체조성 변화)

  • Kim Pyong Kih;Jeon Joong-Kyung;Huh Hyung Tack;Jo Jae-Yoon
    • Journal of Aquaculture
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    • v.9 no.4
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    • pp.395-407
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    • 1996
  • A feeding experiment for 160 days was conducted to evaluate effects of dietary soybean meal (SBM) on the apparent digestibility, ammonia excretion and body composition of rainbow trout (Oncorhynchus mykiss). Seven diets containing $0\~70\%$ SBM were formulated based on iso-nitrogenous and iso-caloric basis of $44\%$ crude protein and approximately 4,200 Kcal/kg of gross energy. To evaluate the nutritional utilization of the SBM diets at the end of 60 and 120-day rearing with test diets, digestion rates of protein and lipid of SBM diets were measured. Fish fed the diets containing above $46\%$ SBM showed higher apparent digestibility for protein, but lower for lipid than did fish fed the control diet in both trials. Protein and fat contents in the carcass were similar for all experimental fish, except for fish fed $58\%$ and $70\%$ SBM which showed lower fat content than the others, but composition of fatty acid and amino acids were not affected by dietary SBM levels. Gill and urinary post-prandial ammonia ($NH_3-N$) excretions were measured at 12 and 24 hours after single feeding of the experimental diets. Excretions of $NH_3-N$ measured after 24 hours were almost 1 times higher than those measured after 12 hours. Total excretion fer the 24 hours by fish fed $70\%$ SBM was $15\%$ higher than that of fish fed the control diet. Results of present study may suggest that the rainbow trout which were fed above $34%\;or\;46\%$ of SBM diet showed a decrease gradually in lipid bioavailability compared to the control group.

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THE UTILIZATION OF DIETARY PROTEIN BY YOUNG RAINBOW TROUT (무지개송어의 사료단백질 이용에 관한 연구)

  • KIM Yong Geun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.7 no.4
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    • pp.209-214
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    • 1974
  • The utilization dietary protein in young rainbow trout was investigated when feeded with the diets of controlled levels of casein and lipids for 12 days. The composition of the diet is shown in Table 1. Body weight gain was in proportion to the protein content in diet up to $40\%$, ana the maximum was obtained with $40.4\%$ of crude protein (Fig. 1, Table 2). On the other hand, the accumulated protein in body also showed almost maximum value around 40n of protein level in the diet and: the change of accumulated protein showed a little with more-protein level(Fig. 3, Table 9). The protein accumulation rate (protein accumulated/protein consumed) showed its highest value at about $40\%$ of protein level, while the protein utilization value (protein accumulation rate x protein content of diet) attained its highest value at $54.8\%$ of protein level (Table 3). With the above results it is observed that the requirement of dietary protein for young rainbow trout is about $40\%$ of protein in the diet when casein is used as the sole protein source. In protein efficiency ratio (PER) the lower protein level in the diet, the higher PER yields and the more the quantity of protein increases, the more PER decreases and its relation could be figured out as an equation of y=4.91-0.034x (Fig.4), Nose measured PER utilizing the diet which result of this, it is reported that PER rate of casein dropped within the extent of $25\%$ protein in diet. The reason why such a different PER rate appeared at the low protein level is revealed as the carbohydrate is low but the lipid high in capacity of utililzing nutrients for rainbow trout. The relation between the protein content of diet and the conversion factor, feed efficiency were determined and the results are shown in Fig. 2.

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Irradiation Preservation of Korean Fishes -Part. II Radurization of Freshwater Species- (방사선(放射線) 조사(照射)에 의한 한국산(韓國産) 어류(魚類)의 품질보존(品質保存)에 관(關)한 연구(硏究) -제2보(第2報) 민물어종(魚種)(잉어 및 무지게 송어)의 방사선조사(放射線照射)-)

  • Chung, Jong-Rak;Kim, Su-Il;Lee, Min-Chul
    • Korean Journal of Food Science and Technology
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    • v.8 no.3
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    • pp.161-171
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    • 1976
  • The meat samples of carp (Cyprinus carpio, Linne) and rainbow trout (Salmo gairdnerii) packaged in aluminum pouches with polyethylene adjuvant were exposed to gamma radiation of doses up to 1.5 Mrad for the purpose of determining optimum dose range required to bring about a significant storage-life extension at refrigerated temperatures. The maximum permissible dose for carp was determined to be 1.5 Mrad and that for rainbow trout 0.2 Mrad, while the optimum dose was 0.25 Mard and 0.05 Mrad, respectively. By irradiating them at each optimum dose, the practical storage-life of carp could be extended from one week to five at both $0^{\circ}\;and\;5^{\circ}C$ and that of rainbow trout from one week to 3-4 weeks at $0^{\circ}C$ and from 3 days to 14 days at $5^{\circ}C$. The carp meat suffered from extensive drip loss during the post-irradiation storage and it could be reduced effectively by dipping the samples into 10% polyphosphate solution prior to the radurization treatment. The rainbow trout was highly radiosensitive, while carp appeard to one of promising species to be radurization treated for the purpose of extending storage-life at refrigerated temperatures.

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콘텐츠연재 / '원소스 멀티유스'의 극대화로 콘텐츠에 활력을..

  • Im, Eun-Mo
    • Digital Contents
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    • no.12 s.91
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    • pp.40-45
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    • 2000
  • 반도체 기술의 발전에 따라 하루가 다르게 디지털 콘텐츠 시장이 변화하고 있다. 여기에 발맞추어 우리 소비자 니즈도 무지개의 빨주노초파남보처럼 다양하게 요구되고 있어 변화하지 않음이 이상할 정도이다. 그래서 다양한 목소리를 내고 있는 60억 지구촌 소비자를 위해 진수성찬을 준비하면서 지난 호에는 7대 메뉴가운데 방송, 영화, 게임 등 빨주노만으로 성찬을 만들었다. 이번호에서는 남은 초파남보로는 인터넷, 위성교육, 모바일 콘텐츠, 그리고 인터넷 광고 등으로 마저 잔치상을 준비할까 한다.

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콘텐츠연재 / '원소스 멀티유스'의 극대화로 콘텐츠에 활력을...

  • Im, Eun-Mo
    • Digital Contents
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    • no.11 s.90
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    • pp.20-24
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    • 2000
  • '각종 미디어에 담긴 내용물'로 개념 정리된 디지털콘텐츠가 '꺼리정보' 또는 '꺼리문화'의 집합체로 떠오르면서 이제는 고유명사의 자리를 내주고 보통명사로 감등된 대신 자연스럽게 콘텐츠 산업을 형성하기 시작했다. it기술에 힘입어 광속의 디지털 경제체제가 형성되면서 하루가 다르게 콘텐츠 산업은 비즈니스 실제로 모습을 드러내고 있다. 마치 무지개가 찬란한 일곱 색깔로 치장하듯 콘텐츠 산업의 7대 메뉴는 이제 돈이 되는 빛깔로 치장하면서 새롭게 태어나고 있다.

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역사속 과학인물 - 고려 명종때의 과학자 '권경중'

  • Park, Seong-Rae
    • The Science & Technology
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    • v.32 no.5 s.360
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    • pp.86-87
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    • 1999
  • 고려 명종때의 과학자이며 사학자였던 권경중은 상서 지제고 등 벼슬에 오른 사람으로 '고려사'에 소상하게 소개되고 있다. 권경중은 신선술에 빠져있던 사람으로 이규보 등과 함께 1186년 '명종실록'을 편찬하기도 했다. 그는 일식과 월식을 재이로 풀이했는데 일식은 내란과 가뭄ㆍ홍수의 징후로 보아왔으며 무지개ㆍ흙비ㆍ안개 등도 재이를 몰고다닌다고 주장해 왔다.

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Chromosomal Polymorphism in Diploid and Induced Triploid Rainbow Trout, Oncorhynchus mykiss (양식산 무지개송어 (Oncorhynchus mykiss) 2배체 및 유도된 3배체의 염색체 다형현상)

  • Kim Dong Soo;Kim Jong-Man;Park In-Seok
    • Journal of Aquaculture
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    • v.3 no.2
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    • pp.145-153
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    • 1990
  • Genetic analysis of rainbow trout populations in hatchery stocks is important in order to develop a strategy for their management. In this study, chromosome number and polymorphisms of diploid and artificially induced triploid rainbow trout are discribed. The relation-ship between chromosomal polymorphism and their economic values for the aquaculture industry are also discussed.

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Early Growth and Gonadal Development of Triploid Rainbow Trout, Salmo gairdneri (3배체 무지개송어의 초기성장과 생식소 발달에 관하여)

  • KIM Dong Soo;KIM In-Bae;BAIK Yun Gul
    • Journal of Aquaculture
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    • v.1 no.1
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    • pp.41-51
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    • 1988
  • Experiments were carried out to examine early life history, growth and gonadal development of triploid rainbow trout to evaluate their suitability for commercial aquaculture. A high degree ($96.5\%$) of triploid fish was induced when the fertilized eggs were treated with heat shock. Mean embryonic development time of triploids was slightly shorter than diploids and survival rate of triploid at swim-up was $80.2\%$ relative to control. Erythrocytes and their nuclei of triploid were larger in both axes than those of diploid. Triploid rainbow trout showed improved growth relative of diploid after 1 year of age. Histological analysis of the gonads revealed sterility in triploid fish.

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