• Microbiology and Biotechnology Letters
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• v.41 no.4
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• pp.398-406
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• 2013

For the maximal production of aphicidal metabolites produced by the Beauveria bassiana Bb08, statistical methods such as the Box-Behnken experimental design and response surface methodology were used. The fungal culture filtrate was sprayed towards 3-star aphids and the mortality was examined. After the statistical analysis of the aphid mortality, the optimal culture conditions were found to be a culture temperature of $26.2^{\circ}C$, medium pH 5.9, flask shaking speed of 209.0 rpm, and culture time of 5.9 days. The expected mortality on days 4, 5, and 6 after spraying the filtrate on to the aphids were 76.8%, 84.9%, and 89.4%, respectively. All 4 factors of the culture conditions significantly affected the production of the aphicidal metabolites, and the order of significance was temperature, pH, culture time and shaking speed.

• Korean journal of applied entomology
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• v.48 no.1
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• pp.87-94
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• 2009

KNI3126 which is the eco-friendly material made by plant extracts - neem and matrine - have been evaluated for efficacies against 6 main pests and toxicity on natural enemies. Insecticidal efficacies of KNI3126 against plant hopper were above 95% at 5 days after treatment (DAT), whereas pesticidal effects against the cotton aphid were above 95% at 3 DAT and slightly decreased at 5 DAT. Insecticidal efficacy against the palm thrips resulted in lower control value as 68.1% than that of chemical insecticides. KNI3126 showed more than 95% control value against diamond back moth, suggesting that it could suppress the population of pest hard to control as eco-friendly material. Mortalities against two-spotted spider mite were about 80% at 1 DAT and over 90% at 5 DAT, respectively. For evaluation of the toxicity on natural enemies, effect against predatory natural enemy was classified as moderate selective toxicity based on the criterion of International Organization of Bio-Control (IOBC), whereas against parasitic natural enemies was found to be relatively safe. Repellent effect was weak against plant hopper, but strong against two-spotted spider mite. In the toxicity test for safety, KNI3126 was classified as the lowest level at acute oral, acute dermal and fish toxicity test and did not induce the irritancy at skin irritation test and eye irritation test.

• Korean Journal of Plant Tissue Culture
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• v.28 no.6
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• pp.297-304
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• 2001

Scrophularia buergeriana Misrule has been contaminated with various pathogens in condition of field and storage period. This study was carried out for production of multiple stress resistance plant containing disease resistance that CGST gene expressed in transgenic Scrophularia buergeriana Misrule genome. Glutathione S-Transferases (GSTs) detoxify endobiotic and xenobiotic compounds by covalent linking of tripeptide glutathione to hydrophobic substrate. GST enzymes have been identified and characterized in insects, bacteria, and many plant species. A cDNA clone of GST was introduced into Scrophularia buergeriana Miquel by transformation with Agrobacterium tumefaciences. In coporation of the CGST gene into S. buergeriana Misrule was confirmed by PCR analysis of genomic DNA. Influence of exposure to darkness on the regeneration potential and transformation frequence were assessed. The activity of GST in transgenic plants was two times higher than that of non-transgenic plants. As a result of anti-microbe assays, the crude extract protein of transgenic plants showed the antimicrobial effects higher than control plants.

• Korean Journal of Childcare and Education
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• v.11 no.3
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• pp.25-44
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• 2015

The aim of this research is to provide more proper teaching practicum chances through getting practical implications by pre-service early childhood teachers' perception of teaching practicum through investigating pre-service early childhood teacher's perception of teaching practicum using metaphors. Participants were 121 pre-service early childhood teachers'. They were early childhood education freshmen and sophomores who did not experience teaching practicum. The opened survey expressing metaphors about teaching practicum was conducted. The results showed that participants suggested 114 metaphors and those were coded and divided. The results were categorized into 11 types including 'It is hard but also fruitful.', 'It is a new experience.', 'It is hard and difficult.', 'It is meaningful and important.', 'I am afraid and worried.', 'I learn a lot.', 'I have to go though it.', 'I have to make an effort.', 'It is pleasant.', 'I am evaluated.' and 'others'. We expect that this research will give a chance to review what the teaching practicum means to pre-service early childhood teachers and make plans for pre-service early childhood teachers to prepare the teaching practicum with proper perception.

• Korean Journal of Organic Agriculture
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• v.17 no.2
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• pp.227-236
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• 2009

This research was carried out to investigate the seasonal occurrence of major pests and the effects of releases of natural enemies for biological control of the major pests in eggplant greenhouse. A total of 8 pest species in 7 families were identified. Among these pests, Myzus persicae, Aphis gossypii, Frankliniella occidentalis, Tetranychus urticae and Trialeurodes vaporariorum were the dominant species in eggplant greenhouse. Two aphid species occurred mainly from May to June, but their populations decreased rapidly from July. The population density of F. occidentalis was high from June to July and T. urticae and T. vaporariorum were abundant from July to September during the growing season of eggplant. In the trials of biological control of pests, aphids could be suppressed within the range of $87{\sim}97%$ by two times releases of Chrysopa pallens eggs. F. occidentalis and T. urticae could be suppressed within the range of $76{\sim}90\;and\;87{\sim}91%$ by three times releases of Orius sauteri adults, respectively. The releases of C. pallens eggs and O. sauteri adults were as effective as three times applications of insecticides for the control of aphids, F occidentalis and T. urticae from May to September.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.2
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• pp.111-115
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• 1989

In order of remove Cd by plants cultivation in the cadimum contamination soil, six fibers, four flowering and two sapling plants were cultivated at Cd treated soil, adjusted to 15 ppm. The Cd contents of plants and absorbed Cd concentration in plant, and residual Cd in soil were analyzed. The results obtained are as follows: 1. The Cd content in marigold was the highest, 161.7ppm, 22.5 times higher than rice. The Cd contents in plants was in the order of 58.3 ppm in sedge, 58.0 ppm in coreopsis, 43.5 ppm in sunflower, 38.3 ppm in scruber, and 31.9 ppm in flax. 2. The absorbed Cd in Popuaus alba x P. glandulosa was the highest, 24.11 mg/pot, 35.5 times higher than rice. The absorbed Cd by plants were in the order of 10.86 mg/pot in italianpoplar, 9.61 mg/pot in marigold, and 9.04 mg/pot in kenaf. 3. The residual Cd contents was the lowest in Popuaus alba x P. glandulosa soil and cotton followed. 4. Absorbed Cd dontent in plants was negatively correlated with the residual Cd concentration in soil.

• Journal of Korean Medical classics
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• v.19 no.2 s.33
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• pp.46-52
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• 2006

${\ulcorner}$황제내경(黃帝內經)${\lrcorner}$ 내함한의학이론(內含韓醫學理論) 기중삼응삼양적내용비상난(其中三陰三陽的內容非常難) 기원인사(其原因是)${\ulcorner}$황제내경(黃帝內經)${\lrcorner}$ 중삼음삼양적내용비상복집(中三陰三陽的內容非常複雜), 특별시삼음삼양적시간배속매편화일양(特別是三陰三陽的時間配屬每篇不日樣) 본론문통과관우(本論文通過關于子) ${\ulcorner}$황제내경(黃帝內經)${\lrcorner}$ 중삼음삼양적시간배속진행연구(中三陰三陽的時間配屬進行硏究), 득출여하결론(得出如下結論), 운기지주기순서춰시오행(목화토금수)순서(運氣之主氣順序就是五行(木火土金水)順序), 저설명주기변화주요근거형지변화(這說明主氣變化主要根據形之變化), 운기지객기순서취시기지대(運氣之客氣順序就是氣之大小)(일양(一陽)${\longrightarrow}$이양(二陽)${\longrightarrow}$삼양(三陽)${\longrightarrow}$일음(一陰)${\longrightarrow}$이음(二陰)${\longrightarrow}$삼음(三陰))${\longrightarrow}$, 저설명객기변화주요근거기지변화(這說明客氣變化主要根據氣之變化) ${\ulcorner}$소문맥해(素問脈解)${\lrcorner}$ 적삼음삼양월별배속시음관(11월:태음)(的三陰三陽月別配屬是陰關(11月:太陰)${\longrightarrow}$양관(1월:태양)(陽關(1月:太陽)${\longrightarrow}$음합(3월:궐음)(陰闔(3月:厥廠)${\longrightarrow}$양합(5월:양명)(陽闔(5월:陽明))${\longrightarrow}$음추(7월:소음)(陰樞(7月:少陰)${\longrightarrow}$양추(9월:소양)(陽樞(9月少陽). 태양소음시음양세력적기초(太陽少陰是陰陽勢力的基礎), 소이상위표리(所以相爲表裏). 소양소음시음양세력적중간(少陽少陰是陰陽勢力的中間), 소이사위표리(所以杞爲表裏) 태음궐음시음양세력적전성기(太陰歐陰是陰陽勢力的全盛期), 소이상위표리(所以相爲表裏) 차료리적의사반영경락류주(此表養的意思反映經絡流注). 태음양명경류주신체전면(太陰陽明經流注身體前面), 태양소음경류주신체후면( 太陽少陰經流注身體後面 ), 소양궐음경류주신체측면(少陽厥陰經流注身體側面). ${\ulcorner}$령추경맥(靈樞經脈)${\lrcorner}$적12경맥류주순서시태음양면경(的12經脈流注順序是太陰陽明經)${\longrightarrow}$소음태양경(少陰太陽經)${\longrightarrow}$궐음소양경적표리경락위주(厥陰少陽經的表裏經絡爲主). 수선매기류주태음양명경적이유(首先脈氣流主太陰陽明經的理由), 취시후천수곡정미화호흡대기결합이형성경맥지기(就是後天水穀精微和呼吸大氣結合而形成經服之氣). 지후맥기류주소음태양경(之後脈氣流注少陰太陽經), 설명순행태음양명경적후천영양물질전입한열경락이위신(說明盾行太陰陽陽明經的後天營養物質轉入寒熱經絡而爲寫薪), 기차맥기순해궐음소양경적(其次脈氣盾行厥陰少陽經的), 인위한열경락지화기피궐음소양경지풍기조절이후조절조습경락(因爲寒熱經絡之火氣被厥陰少陽經之風氣調節而後調節操濕經絡), 비여작반시(比如作飯時), 화기취시소음태양경(火氣就是少陰太陽經), 풍기취시궐음소양경(風氣就是厥陰少陽經), 정내지반취시태음양명경.(鼎內之飯就是太陰陽明經). ${\ulcorner}$령추음양계일월(靈樞陰陽緊日月)${\lrcorner}$ 적삼음삼양월별배속주요시추관합합관추순서(的三陰三陽月別配屬主要是樞關闔闔關樞順序). 저양배서적원인시개문화관문과정(這樣排序的原因是開門和關門過程). 재춘하(在春夏), 양경락지맥기여개문관문과정(陽經絡之脈氣如開門關門過程), 수착추관합합관추순서순행(隨看樞關闔闔關樞順序盾行), 재추동(在秋冬), 음경락지맥기여개문폐문과정(陰經絡之脈氣如開門閉門過程), 수착추관합합관추순행(隨看樞關闔闔關樞盾行).

• Korean Journal of Weed Science
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• v.16 no.1
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• pp.28-35
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• 1996

Tetrapion(2,2,3,3-tetrafluoropropionate sodium) has commonly been used for reed(Phragmites communis Trin.) control in uncultivated areas, especially in Japan. As an attempt to establish the screening system for selective herbicide controlling reed, tetrapion was tested as a standard herbicide to various weeds and crops including rice in a greenhouse. Symptoms of yellowing, twisting, stunting, and necrosis were observed in the herbicide-treated plants. The herbicide caused a severe damage on all crops examined, except cotton. Both direct seeded and transplanted rice were also sensitive to the herbicide. Its herbicidal activity was generally higher on grass weeds than on broadleaf weeds. It had a higher herbicidal activity with preemergence treatment than with postemergence treatment. In addition, field and greenhouse experiments were conducted to examine the effect of the herbicide on reed control. Its effect varied with the soil type of the treated field and the growth stage of reed. A relatively high dosage of the herbicide was required to control reed. It had an almost same effect on fairygrass(Miscanthus sinensis Anderss) as on reed. However, reed grown in a greenhouse was effectively controlled by the herbicide, having more effect with preemergence treatment than with postemergence treatment as observed in other plants treated with the herbicide. In conclusion, tetrapion has a great potential as a standard herbicide during the herbicide screening for selective reed control.

• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.419-429
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• 2017

We investigated the occurrence pattern of diseases and insect pests and disease control efficacy of organic materials against watermelon powdery mildew in small type watermelon in Eumseong, Chungcheongbuk-do, 2015. The result of this study, the small type watermelon was damaged by diseases and pests such as Didymella bryoniae, Podosphaera xanthii, Aphis gossypii, Tetranychus urticae, thrips, Spodoptera exigua and Spodoptera litura. Among them, the occurrence of P. xanthii, T. urticae and thrips was high. Diseased leaf rate by P. xanthii, a casual agent of powdery mildew, was 27~99.3% in three small type watermelon cultivars in the middle of June. The number of T. urticae per leaf was high from 79.9 to 111 in three small type watermelon cultivars in the middle of June. It showed high number of thrips captured by yellow and blue sticky trap. Highest numbers of yellow sticky trap (407) and of blue sticky trap (774) were detected in the middle and first of June, respectively. The disease control efficacy of mayonnaise, oleic acid and three organic materials against powdery mildew of small type watermelon in fields was evaluated. As thre results, the symptoms of plant disease were effectively reduced by over 60% in the treatments of materials such as sodium bicarbonate 80%, mayonnaise and the extract of Rheum palmatum 1%. The highest control efficacy was 83% in the treatment of sodium bicarbonate 80%. From this study, we had a information of the occurrence pattern of diseases and insect pests in small type watermelon and the treatment of material containing sodium bicarbonate 80% was very effective for controlling against powdery mildew.

• Korean Journal of Environmental Agriculture
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• v.17 no.2
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• pp.122-126
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• 1998

This experiment was conducted to verify the responses of major crop species to saline stress.To determine the saline tolerance of crop species, sevral crop species were cultivated under sand-culture system using Hoagland's nutrient solution whit 200mM NaCl. The crop species showing saline tolerance were cotton(Gossypium indicum), maize(Zea mays), barely(Hordeum vulgare), and wheat(Triticum aestivum) but perilla (Perilla frutescens) and leguminous crops, mung bean(Phaseolus radiatus), azuki bean(Phaseolus angularis) and soy bean(Glycin max) showed very por tolerance. One the typical symptom was the darkening of leaf color due to increase of chlorophyll concentration.Among of the plant families, Fabaceae was the most susceptible but crop species belonging to Poaceae were more proper for cultivating on reclaimed tidal land in the course of desalinazation. It was suggrsted that the crop species belonging to Fabaceae, a sensitive family to soil salinity, must be cultivated when the soil salinity decreased below 10ds/m. To know the critical salinity level for crop growth,salinity of saline soil collected from reclaimed tidal land was adjusted from 10.0ds/m tp41.7ds/m with tap water. It was suggested that the ECs of the soil in which the plant height of each crop spicies was reduced to 50% of control plant were 22.6 and 21.7 ds/m in rice, barley, corn, mung bean, and soy bean,respectively.