• Microbiology and Biotechnology Letters
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• v.23 no.6
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• pp.633-640
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• 1995

As a result of screening the medicinal herbs which selectively control human intestinal microflora, water extract of Akebia quinata Decaisne was proved to have a strong inhibitory activity against the growth of Clostridium pefringens, a major harmful intestinal bacterium. The anti-bacte-rial activity was stable under the thermal treatment at 100$\circ$C for 120 min and in a range of pH 1 to 11. In addition, the water extract of Akebia quinata Decaisne showed the antibacterial activities against five different strains of Clostridia including C. perfringens. On the contrary, the extract did not inhibit the growths of Bifidobacterium, Lactobacillus, Escherichia coli, Enterococcus faecalis. The extract, however, suppressed markedly the growth of Bacteroides fragilis and Staphylococcus aureus in vitro. Alike in the mixed culture inoculated with human feces as starter, in vivo tests using rats showed that the extract tends to increase the numbers of Bifidobacteria and Lactobacilli in the intestinal microflora of rats, whereas those of Clostridia were attenuated.

• Journal of the Korean Institute of Oriental Medical Informatics
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• v.20 no.1
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• pp.1-8
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• 2014

Objectives: This study aimed to find out the origin, efficiency in sasang constitutional medicine and dosage of Mu tong. Methods: Investigation and analysis was been done through 15 literatures like the pharmacopoeia of the five countries in east Asia, the literatures about sasang constitutional medicine and so on. Results and conclusion: The species of Mu tong in Korea is identical with the Mu tong in China, but different from Chuan mu tong and Guan mu tong in China. Mu tong is a kind of medicine using by kidney can be use at most 12 qian (about 45g) a day when treating heat closing in small intestine, edema, headache, dysentery and difficult urination on Soyangin(SY).

• Journal of Pharmaceutical Investigation
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• v.9 no.2
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• pp.22-30
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• 1979

Effects of Akebiae Lignum, whose scientific name is Akebia quinata Decaisne, on the blood pressure were investigated with EtOH extract in whole and spinal rabbits. Akebia Lignum EtOH extract (AEE), when given intravenously, produced a fall in blood pressure not only in whole rabbit but also in spinal rabbit and AEE administered into a lateral cerebral ventricle of whole rabbit did not elicit a fall in blood pressure. The depressor responses of the whole rabbit to intravenous AEE were weakened by treatment of the animals with atropine and chlorisondamine but not by vagotominijation, phentolamine avil, and then the depressor action causing by AEE in the whole rabbit was not affected by pretreatment of physostigmine which is cholinesterase inhibitor and of hemicholinium which blocks acetylcholine synthesis by interfering with choline uptake in nerves. These observations suggest that the hypotensive action of AEE of which component is not affected by cholinesterase is due to direct action at parasympathetic receptor.

• YAKHAK HOEJI
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• v.22 no.4
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• pp.207-214
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• 1978

Influence of Akebiae Lignum on the renal function of the dog was observed with water-extract, utilizing clearance technique. Akebiae Lignum water-extract (AWE) given intravenously in does of 3.0mg/kg and 30.0mg/kg elicited a marked antidiuretic effect and produced a distinguished decrease of glomerular filtration rate and renal plasma flow as reduction of sodium and potassium excretion in urine, positive free water clearance and osmolar clearance. AWE infused into a renal artery in doses of 0.03mg/kg/min and 0.1mg/kg/min exhibited identical results to the intravenous action confined only to the infused kidney. These results suggest that AWE elicits antidiuresis in the dog by decreasing glomerulor filtration and renal plasma flow, which are caused by the constriction of vas afferens in the glomeruli.

• Food Science and Preservation
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• v.12 no.6
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• pp.565-571
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• 2005

This study was to develope health promoting and better quality of Kochujang. Three kinds of medicinal plants, Angelica acutiloba (AA), Akebia quinate decaisne (AQ), Caragana chamlagu (CC) were extracted with water. Hanbang Kochujang was prepared by adding water extracts of medicinal hem to the ingredients of Kochujang. Quality characteristics of Hanbang Kochujang were evaluated by its water content, color and sensory evaluation during storage at $5^{\circ}C$: and $20^{\circ}C$ for 90 days. Moisture contents of Hanbang Kochujang before storage were $33\~38\%$ and increased to $48\~51\%$ at the end of storage. Color values(L, a and b) of Hanbang Kochujang were decreased as the progress of storage period at $5^{\circ}C$ and $20^{\circ}C$. Redness (a) values of Hannbang Kochujang with Akebia quinate decaisne (KAQ) and Caragana chamlagu (KCC) were lower than that of control Kochujang (KC) during storage at $20^{\circ}C$(p<0.05). Yellowness(b) values of Hannbang Kochujang with Caragana chamlagu (KCC) was lower than that of control Kochujang (KC) during storage at $5^{\circ}C$(p<0.05). From sensory evaluation, preference was higher in Hanbang Kochujang (KAA, KAQ and KCC) stored at $20^{\circ}C$ than that stored at $5^{\circ}C$ during 60 and 90 days of storage. Sensory evaluation scores of color, sweet taste and overall preference were the highest in Hanbang Kochujang added Caragana chamlagu (KCC) during storage at $5^{\circ}C$ and $20^{\circ}C$ for 90 days (p<0.05).

• The Journal of the Korea Contents Association
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• v.19 no.1
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• pp.242-255
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• 2019

In this study, we examined the antimicroboal effect against Actinobacillus actinomycetemcomitans and Prevotella intermedia which were the bacteria causing the Periodontopathic by using 34 types of natural plant extracts. Therefore, this study measures growth inhibition activity and Minimum Inhibition Concentration (MIC) of a sample extract with the use of organic solvent extracts in order to analyze the antibacterial effect of natural plant extracts on periodontopathic bacteria. Each of the 34 types of natural plant extracts were extracted by using the ethanol, and subsequently, the size of growth inhibition zone(clear zone, ㎜) of respective extracts were measured through the disk diffusion method. As a result, it was found that the growth inhibitory activity was found for A. actinomycetemcomitans, which is the bacteria causing the Periodontitis, in 13 types of natural plant extracts such as Raphanus sativus, Akebia quinata, Paeonia lactiflora, Belamcanda chinensis, Inula britannics, Houttuynia cordata, Forsythia saxatilis, Gentiana macrophylla, Melia azedarach, Scutellaria baicalensis, Coptis chinensis, Phellodendron amurense, Kalopanax Pictus, etc. In the case of P. intermedia, the growth inhibitory activity was found in 13 types of natural plant extracts such as Raphanus sativus, Angelica acutiloba, Akebia quinata, Belamcanda chinensis, Inula britannics, Houttuynia cordata, Cinnamomum cassia, Aster tataricus, Melia azedarach, Scutellaria baicalensis, Coptis chinensis, Phellodendron amurense, Kalopanax Pictus etc. For A. actinomycetemcomitans, anti-bacterial effect was exhibited in Belamcanda chinensis, Cinnamomum cassia, Kalopanax Pictus, Phellodendron amurense, Coptis chinensis. The Coptis chinensis showed the most excellent growth inhibitory activity in all organic solvent fragment, while P. intermedia showed the growth inhibitory activity in Belamcanda chinensis, Cinnamomum cassia, Meliaazedarach, Phellodendron amurense, and Coptis chinensis.

• The Korea Journal of Herbology
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• v.20 no.4
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• pp.77-82
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• 2005

Objectives : In order to distinguish Akebiae Caulis and Aristoiochiae Manshuriensis Caulis, their external and internal states were examined by microscope. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results : 1. Akebiae Caulis has stone cells and fiber bundles in its pericycle part, which include square crystal of calcium oxalate. Outside of neogenesis has a lot of cells with square crystal of calcium oxalate. 2. Aristoiochiae Manshuriensis Caulis has a little lignified fiber bundles in its pericycle part, and parenchymatous cell has delicate starch grains and druse of calcium oxalate. Conclusions : In conclusion, internal forms of Akebiae Caulis and of Aristoiochiae Manshuriensis Caulis are different in that each includes square crystal of calcium oxalate and druse of calcium oxalate respectively, so that it may be possible to distinguish them with optical microscope.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1997.04a
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• pp.99-99
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• 1997

현재까지 NSAID 및 SAID의 사용으로 급성염증의 경우는 잘 조절되고 있으나, 류마티스 관절염과 같은 만성염증성 질환은 극복하지 못하였다. 뿐 만 아니라, 상기의 약물들의 장기간 사용으로 인한 부작용이 문제되고 있다. 그러므로, 만성염증성 질환의 치료를 위한 새로운 계열의 항염증제 개발이 시급하며, 많은 연구자들이 여러 가지 식물추출물을 이용하여 신약개발의 가능성을 타진하고 있다. 이의 일환으로, 본 연구에서는 고전문헌에서 사용된 식물들을 대상으로 하여 Rat의 류마티스 관절염 model을 이용하여 그들의 항염증작용을 연구하였다. 여정자 및 등줄나무를 포함한 27종의 식물을 이용하여 각 methanol 추출물을 조제하고, 매일 경구로 투여하였다 (200 mg/kg/day). 류마티스성 관절염은 rat의 족부에 Mycobacterium butyricum (0.6 mg/rat)을 주사하여 유발시켰고, 2차부종의 억제를 추출물의 활성으로 판정하였다. 그 결과, 27종의 식물중 목통, 마황 및 산두근이 2차부종을 유의성있게 억제하였으며, adjuvant 주사 후 16일에 억제율이 각각 22%, 36%, 13%로 나타났다. 산두근을 분획하여 재검정한 결과 50 mg/kg/day의 용량으로 투여시 EtOAc 및 n-butanol 분획에서 억제능이 나타나, 이들 분획을 대상으로 활성물질의 분리를 계속하고 있다.

• YAKHAK HOEJI
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• v.59 no.3
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• pp.120-126
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• 2015

Akebiae caulis have been used in folk medicines for diuretic, menstrual pain, and diuretic pain. It has been also resolved nephritis and cystitis. In this study, we isolated three phenolic compounds from 70% methanol extract using the open column chromatography. These isolated compounds which were 2-(3,4-dihydroxyphenyl)-ethyl-${\beta}$-D-glucopyranoside, 5-O-caffeoylquinic acid, and syringoylglycerol 2-O-${\beta}$-D-glucoside were determined by physico-chemical apparatus. Furthermore, we conducted DPPH and ABTS assay in order to screen the antioxidant activity of isolated three compounds. Also, we developed a rapidly HPLC-UV analysis method of two phenolic compounds (2-(3,4-dihydroxyphenyl)-ethyl-${\beta}$-D-glucopyranoside, 5-O-caffeoylquinic acid) for evaluating the Akebiae Caulis collected 30 samples from different regions. From the experiments, all three isolated compounds showed the significant antioxidant activity. We suggested that the content criteria of marker compounds were shown by a simple and rapid HPLC-UV method. The contents respectively were 0.009% (2-(3,4-dihydroxyphenyl)-ethyl-${\beta}$-D-glucopyranoside) and 0.036% (5-O-caffeoylquinic acid).

• Korean journal of applied entomology
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• v.33 no.4
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• pp.225-229
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• 1994

The methanol extracts from 30 species of oriental medicinal plants belonging to 24 families were tested for their lhicidal and antifeeding activit~es against diamondback moth (Plutello xylostella L) and tobacco cutworn (Spodoptera litura F.) by a leaf-dipping method at a concentration of 5, 000 ppm. The methanol extract from Copti chinensis only showed a potent larvicidal activity against P xylostello. Strong antifeeding activity against P. xylostello was observed from the extmds from Platycodon grandiflorurn, Codonopsis pilosula, Asomm sieboldii, Rhus chinensis and Uthospermum erythrorhizon And a potent antifeeding activity against S. liturn was obtained from Akebia quinata and Equlsetum hyemale extracts. A significant antifeeding activity against both species was obtained from R chinensis and C. chinensis extracts.