• The Journal of the Korea institute of electronic communication sciences
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• v.17 no.4
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• pp.709-714
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• 2022

The color change of the success of the steam sterilization indicator used in steam sterilization is currently being read by relying on the human eye. The result of color change on the success of sterilization can only be accurately read and judged by trained personnel. Developed an automatic reading system for steam sterilization indicators for the current human-dependent system, and developed an automatic reading device system for reading methods that were previously relied on with the human eye to develop a system that is more accurate and easier for anyone to read.

• Proceedings of the Korea Water Resources Association Conference
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• 1992.07a
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• pp.506-512
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• 1992

PET병에 넣어 시판되고 있는 일부 물중에서 일반세균과 대장균이 문제되고 있어 이러한 균을 멸균하기 위한 실험을 Cobalt-60과 X-ray를 이용하여 효과를 비교했다. 그 결과 Cobalt-60에 의해서 1.1KGY의 선량으로 물속의 모든 균은 멸균되었으나 X-ray에 의한 실험(56KVP 1mA 9초까지의 조사)에는 효과가 없었다. 앞으로 장기간 보관식품은 Cobalt-60에 의한 조사가 효과적이라고 생각되며 아울러 조사된 물은 인체에 하등의 영향을 주지 않는다.

• Journal of the Korean Society of Radiology
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• v.14 no.6
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• pp.833-839
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• 2020

Globally, infection prevention and social awareness have been greatly changed by the severe acute respiratory syndrome coronavirus, and as a result, the infection control guidelines and procedures for patients with high exposure to hospital-acquired infection are further strengthened and management and monitoring are more thorough. In order to prevent infection, sterilization should be carried out with the highest priority, and we will find a sterilization method that is low in cost, easy to install, and easy to operate, to present appropriate sterilization effects. In this study, the UV sterilizer was used to contaminate the caries bacteria with an output of 4 W and irradiation time of 60, 150, and 300 sec, and the laser was irradiated with outputs of 0.8 and 1.5 W at wavelengths of 266 and 355 nm, respectively. Ultraviolet sterilizer showed safety in infection prevention at over 150 sec, and laser showed safety in prevention at 1.5 W, 0.8 W, and 266 nm. As a result, the higher the output and the wavelength closer to 253.7 nm, the better the sterilization effect.

• The Korean Journal of Pesticide Science
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• v.3 no.2
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• pp.8-18
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• 1999

The degradation of a fungicide, $^{14}C$-propiconazole, in sterile and nonsterile soil from different depths was investigated. $^{14}C$-propiconazole plus propiconazole standard was treated on the soil at the rate of 7.55 mg/kg and the soil was incubated at $25^{\circ}C$ for 20 weeks. The amounts of $^{14}CO_{2}$ solvent extractable and non-extractable $^{14}C$, and degradation products of $^{14}C$-propiconazole were investigated during incubation time. The relative amounts of $^{14}CO_{2}$ released in the sterile and nonsterile soils were ranging from 0.7 to 1.3% and from 4.8 to 7.6% of applied $^{14}C$, repectively. The amounts of solvent non-extractable residues in the sterile and nonsterile soils were ranging from 11.2 to 22.1% and from 22.2 to 41.9% of of applied $^{14}C$, repectively. The amounts of solvent non-extractable residues were increased with incubation time and most of $^{14}C$ were detected in the humin fraction. The hydroxylated and ketone compound were confirmed as a degradation products of propiconazole by GC/MS analysis, whereas parent compound was detected in sterile soil, which suggested that propiconazole was not degraded biologically under the sterile soil. From the results of volatilization, mineralization and degradation of propiconazole, propiconazole was stable chemically and bilogically in soil.

• Restorative Dentistry and Endodontics
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• v.30 no.2
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• pp.121-127
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• 2005

A variety files made of stainless steel (S-S) or nickel-titanium (Ni-Ti) are used during endodontic treatment. The purpose of tt)is study was to evaluate the corrosion susceptibility of S-S and Ni-Ti endodontic files. Three brands of files were used for this study: $K-flex^{(R)}$ S-S files (Maillefer, USA), $Profile^{(R)}$ Ni-Ti files (Maillefer, USA), $K-3^{(R)}$ Ni-Ti files (SybronEndo. USA). 120 files of each brands (21mm, ISO size $\#20$) were divided into 12 groups according to 1) sterilization methods using Autoclave or Ethylene Oxide (E-O) gas, 2) Irrigation solutions using $5.25\%$ NaOCl or Saline, 3) the number of sterilization (1, 5, 10 times), After above procedures, each of the files was inspected by three examiners with a light microscope and camera at X25. Each file was judged and ranked according to the following criteria: 0;, no corrosion, 1; mild corrosion, 2; moderate corrosion, and 3; severe corrosion. The files of high score were examined under the Scanning Electron Microscope. Data were statistically analyzed with the Kruskal-Wallis test (p < 0.05). Most of the ten time-autoclaved files had showed mild to moderate corrosion. But, one or five time-autoclaved files did not show corrosive surface. NaOCl treatment and E-O gas sterilization did not influence on corrosion. There was a significant difference in corrosion susceptibility between sterilization methods and the number of autoclaving. However, there was no significant difference between brands and file materials.

• Korean Journal of Microbiology
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• v.50 no.4
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• pp.345-350
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• 2014

The aim of this study was to investigate the effect of high temperature on the viability of probiotic organisms (Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae) mixed with animal feed under controlled conditions by simulating a farm feed bin in the summer. Following inoculation of probiotics into the feed, the pH and probiotic viability were monitored during an 8-day incubation at room temperature. Sterile and non-sterile feeds displayed different patterns of pH changes, with increased pH in non-sterile feed at 2 days, but a pattern of decreasing pH at 4 days. The viabilities of S. cerevisiae and B. subtilis after mono/co-inoculation were maintained without substantial changes during the incubation, whereas L. plantarum viability tended to decline. In both non-sterile and sterile feeds, the probiotics were maintained or grew without any antagonistic effects. Probiotic viability was also tested upon a shift to high temperature ($60^{\circ}C$). There was no distinct change in pH between sterile and non-sterile feeds after the temperature shift. L. plantarum and S. cerevisiae could not survive at the high temperature, whereas B. subtilis displayed normal growth, and it inhibited the growth of contaminant microbes. Fungal growth was not observed in non-sterile feed 2 days after supplementation with B. subtilis. Therefore, heat resistant B. subtilis could be safely used in feed bins to inhibit microbial contamination, even at high temperatures. The prevention of elevated temperature in feed bins is necessary for the utilization of L. plantarum and S. cerevisiae during the summer season.

• YAKHAK HOEJI
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• v.29 no.5
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• pp.305-315
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• 1985

Validation이란 용어는 1970년대에 우리나라에 GMP개념이 도입되면서 처음 사용되기 시작하여 지금은 흔히들 사용하면서도 그 개념은 아직도 정확히 이해되고 있지 않은것 같다. 제약공업에서의 validation의 적용범위는 아주 광범위하며 제조기기, 제조공정, 조직, 인원 등에 그 개념을 도입하여 관리하고 있다. 따라서 본 란에서는 validation의 일반적인 개념을 서술한 후 액상제제 특히 무균제제에 대한 calidation중 멸균공정에 대하여 상술하고자 한다.

• Korean Journal of Agricultural Science
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• v.9 no.2
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• pp.556-561
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• 1982

These studies were carried out to investigate the freshness and bacterial counts of ultrahigh- temperature sterilized market milk which were treated and distributed in three districts; Cheonan, Pyungtak and Suwon in Korea, and to elucidate theirs keeping qualities when stored in refregerator and at room temperature. And the results were obtained were as follows; 1. The acidities in milk from three plants after stored at $5^{\circ}C$ were retained 0.16% up to 6 weeks and 0.18% up to 10 weeks. And negative reactions were appeared for alcohol and boiling tests also up to 10 weeks. 2. After milk were stored at $25^{\circ}C$, the acidities were 0.18% up to 9 weeks and also negative reaction were appeared for both alcohol and boiling tests up to 9 weeks. 3. When milk were stored at $5^{\circ}C$, no thermoduric and coliform bacteria were detected from all samples. And the total viable number of organisms and the number of psychrophilic bacteria were $<10/m{\ell}$ for 5, 6, 7 and 10 weeks respectively. 4. Also thermoduric and coliform bacteria were not detected after milk were stored at $25^{\circ}C$ for 10 weeks. And the total viable number of organisms and the number of psychrophilic bacteria were $<10/m{\ell}$ after from 2 weeks to 10 weeks.

• Korean Journal of Environmental Biology
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• v.28 no.3
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• pp.150-157
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• 2010

Biodegradation behavior of poly(butylene succinate-co-butylene adipate) (PBSA) was examined when PBSA was buried in the natural soil and the soil inoculated with Burkholderia cepacia after sterilization. After 80 days of the soil burial test at room temperature, the PBSA film buried in the natural soil lost 34.0% of its intial weight, while the same film lost 59.2% of its initial weight when buried in the sterile soil inoculated with B. cepacia. The optical and SEM observations of the surface morphology of the PBSA film also indicated that the surface erosion and rupture took place faster when the film was buried in the sterile soil inoculated with B. cepacia compared to the film buried in the natural soil. Viable cell number in the natural soil and that the sterile soil inoculated with B. cepacia increased by a factor of 6~7 and 10~14, respectively as compared to the initial viable cell number.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.02a
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• pp.41-41
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• 2010

상온에 준하는 저온의 플라즈마를 발생시키는 장치들이 개발되면서, 저온 플라즈마와 생체조직간의 상호작용에 대한 연구가 큰 관심을 끌고 있다. 플라즈마에서 발생되는 다량의 이온과 활성종, 그리고 UV 등이 박테리아나 세포들과 작용함으로 해서 암세포 사멸, 치아 미백, 박테리아 살균/멸균, 지혈등의 효과들이 나타나고 있으며, 이러한 효과들을 극대화할 수 있는 장치 개발과 플라즈마와 생체조직간의 상호작용 메카니즘을 규명하는 것이 중요한 이슈가 되고 있다. 나노 금입자를 암세포의 막단백질인 FAK의 항체와 결합시킨 중합체를 만들어서, 암세포 표면에 나노 금입자붙이고, 플라즈마를 조사했을 때, 나노 금입자가 부착되지 않았을 경우에 비해서, 5배이상 사멸률이 증가하였다.[1] 변색된 치아에 미백제의 주성분인 과산화수소를 도포하고, 10분간 플라즈마를 조사하게 되면, 과산화수소만 도포했을 때에 비해, 치아 표면의 색이 3배이상 밝아지는 것을 관찰할 수 있었다. 과산화수소를 플라즈마에 노출시켰을 때, 활성종인 OH의 생성이 2배이상 증가하였고, 플라즈마에 의한 OH 생성의 촉진이 치아 미백효과가 증대되는 주된 요인인 것으로 추측된다.[2] 플라즈마에서 발생되는 O, $O_3$와 같은 활성종들은 살균력이 뛰어나기 때문에, 저온 플라즈마를 의료기구의 소독/멸균에 응용할 가능성이 아주 크다. 대장균이나 구강 세균이 플라즈마 처리로 5분이내에 멸균되는 것을 확인하였고, 핸드피스와 같은 의료기구를 오염시켜서 멸균 테스트를 수행하고 있다.