• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.32-32
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• 2018

층층둥굴레(Polygonatum stenophyllum Maxim.)는 백합과(Liliaceae) 둥굴레속(Polygonatum)에 속하는 다년생초본이다. 둥굴레속의 근경은 점액질이 풍부하고 전분질, 아미노산, 알카로이드 등의 성분을 다량 함유하고 있어, 식품학적으로도 우수한 것으로 보고되었다. 그리고 새순과 여린줄기는 식약청 식품원재료서비스에 등록된 식용가능 식물자원이다. 본 연구에 사용된 층층둥굴레는 경기도 황학산수목원에서 2년, 5년생과 종자를 분양받아 종자는 기내에서 발아시킨 후 실험에 사용하였다. 야생의 개체를 무균적으로 기내도입하기 위해 차아염소산나트륨의 농도를 0~1.5%까지 달리하여 멸균한 결과, 유의적 차이는 없으나 1~1.5%처리가 비오염율이 20% 내외로 조사되었다. 층층둥굴레 캘러스 유도에 미치는 옥신류 호르몬의 영향을 알아보기 위해 멸균된 근경을 절단하고 옥신류 호르몬의 종류와 농도가 각각 다르게 처리된 MS배지에 치상하여 배양한 결과, 2,4-D 0.5mg/L 처리구에서 캘러스 유도율이 87%로 가장 높게 조사 되었다. 이러한 결과는 층층둥굴레의 기내배양 방법을 통해 효과적으로 모본과 동일한 층층둥굴레의 대량생산 체계를 만들 수 있는 참고자료로 활용될 수 있고 더불어 관련 산업계의 소재다양화 측면에서 유용하게 사용될 것으로 보여진다.

• Journal of Ginseng Research
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• v.33 no.2
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• pp.139-142
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• 2009

The effect of calcium cyanamide (China-made) soil fumigation on the growth of the ginseng pathogen Rhizoctonia, Pythium), soil microbes, and seed germination of lettuce and radish was investigated. At twice the recommended level (2S0-ppm $CaCN_2$), the growth of Rhizoctonia and Pythium, and the seed germination, were not inhibited. Rather, the effective level was 10,000 ppm. The powder form was more effective than the granular form in inhibiting pathogen growth and seed germination. The lettuce seed was also more sensitive than the radish seed. Calcium cyanamide appearedto decrease the fungi population and to increase Actinomycetes in the soil.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.21 no.2
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• pp.141-150
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• 2014

Purpose: This was a study on the expiration date of Ethylene Oxide (EO) gas sterilization and effects of the environmental factors of temperature, humidity and type of cabinet in sterile goods storage area on the expiration date. Methods: Sterile goods storage areas from 13 departments in one hospital were selected and 455 EO gas sterilization samples were prepared and kept in those areas over the 14 months of the study. Each sample was tested with a microbiological culture in the laboratory every week. If the result was positive, the sample was regarded as contaminated. The researcher visited once a month to check the temperature, humidity and type of cabinet. Results: With the exception of 1 sample which was positive at 56th week. 454 samples were confirmed as negative. The environment of the samples storage area was measured monthly. The annual average temperature was $24.2{\pm}1.6^{\circ}C$, and the mean relative humidity $34.7{\pm}15.2%$. The types of cabinet were 7 open and 6 closed. Conclusion: The results of the microbiological culture at 13 months showed that none of the samples were contaminated. Therefore the hospital's existing Expiration Date can be extended from 6 months to 13~14 months.

• Journal of Dental Rehabilitation and Applied Science
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• v.32 no.4
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• pp.274-279
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• 2016

Purpose: The purpose of this study was to evaluate the transmission of candida in denture by dental polishing lathe. Materials and Methods: Maxillary complete dentures made from the same model were infected with Candida albicans. Polishing wheels were keep in various chlorhexidine solution and distilled water for an hour. The infected dentures were polished by prepared dental polishing lathe with sterile pumice and distilled water. And then sterile maxillary complete dentures were polished with same method. Polishing surface was wiped with a cotton swab and the sample was regrown for checking Candida albicans. Results: All polishing wheel with chlorhexidine resist fungal infection. But the polishing wheel with distilled water is infected with Candida albicans. Conclusion: A chlorhexidine is highly efficient in fungal infection prevention on dental polishing lathe.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.1468-1469
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• 2007

포항가속기 연구소에서는 RIS 프로젝트 사업과제의 일환인 L-band 산업용 전자빔 가속기의 제작 및 기술개발 연구를 철원의 물리기술연고소 주관으로 수행하고 있다. L-band 산업용 전자빔 가속기는 전자빔에 의한 고분자개질 공정, 농수산물의 멸균 및 의료기기의 멸균 등에 적용이 가능하다. 포항가속기연구소에서는 10 MeV급 산업용 전자빔 조사장치에 적용되는 RF증폭기용 펄스 전원장치인 모듈레이터를 설계, 제작을 하였다. 펄스 전원장치인 이 모듈레이터 시스템은 평균 출력 60 kW, 펄스폭 7 us, 펄스반복율 300 Hz의 L-band 상업용 RF 증폭기 전원장치이며, RF 증폭기로 사용되는 클라이스트론은 Thales 사의 TV2022D를 사용하였다. 본 논문에서는 펄스 전원장치인 모듈레이터의 운전과 인터록을 위한 제어장치의 개발과 제작에 대하여 논하고자 한다.

• Korean journal of food and cookery science
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• v.26 no.3
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• pp.335-345
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• 2010

The objective of this study was to model the kinetics of S. aureus survival on high risk foods in school foodservice operations. After inoculating S. aureus ATCC25923 onto the various high risk foods, the effects of competitive microorganism, storage temperatures($25^{\circ}C$, $35^{\circ}C$), and initial contamination levels ($1.0{\times}10^2\;CFU/g$, $1.0{\times}10^5\;CFU/g$) on the growth of S. aureus were investigated. Lag time decreased and specific growth rate increased with a storage temperature ($25^{\circ}C$<$35^{\circ}C$) and with a higher initial inoculation level ($1.0{\times}10^2\;CFU/g$<$1.0{\times}10^5\;CFU/g$). Previously it was shown that S. aureus is a weaker competitor than other organisms, but it proliferates aggressively in a noncompetitive environment. However, in our study, when S. aureus was used to inoculate japchae (glass noodles with sauteed vegetables) and meat ball, the growth of S. aureus was similar and more active with competitive organisms than that without competitive organisms. Regardless of other factors, the initial level of S. aureus was a more significant factor of the growth. High inoculation levels of S. aureus were reached at 6 log CFU/g within 3 hours. An incubation temperature of $35^{\circ}C$ and the animal protein component of menu items also were identified as significant factors influencing the growth of S. aureus. Therefore, the duration of time meals are stored before serving should be considered a critical control point. Food service providers must control time and temperature to insure the safety of cooked foods.

• The Journal of Korean Academy of Prosthodontics
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• v.46 no.1
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• pp.42-52
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• 2008

Purpose: Resonance Frequency Analysis(RFA) technique can be used as an effective method in measuring the implant stability and documenting the clinical results. This technique also determines how stable the implant is before performing a prosthetic practice. Having become one the guidelines of the implant therapy whose final objective is the immediate loading, the $Osstell^{TM}$ mentor is giving a lot of information to the clinicians recently. In this communication, experiments were performed to investigate how reliable the measured ISQ values by $Osstell^{TM}$ mentor are, and to see if those are also stable even after sterilization. As five objectives: 1) How stable measured ISQ values after fixation $Smartpeg^{TM}s$ for 400 times. 2) How stable measured ISQ values after 'attach-detach'$Smartpeg^{TM}'s$ for 400 times. 3) How stable measured ISQ values after clinical sterilization methods. 4) How stable measured ISQ values after repeatedly sterilization in autoclave for 10 times. 5) What is the critical temperature which is lost the magnetism of $Smartpeg^{TM}$. Materials and Methods: Clinical sterilization methods(Autoclave sterilization, Dentistar sterilization, Ultra violet sterilization, Vacuum dry unit sterilization, Boiling water sterilization, combined $H_{2}O_{2}$ and Alcohol sterilization).$Smartpeg^{TM}s$. D3 Block bone($3{\times}9{\times}2cm$). Osstem implant(${\emptyset}4.1$-10mm).$Osstell^{TM}$ mentor. Individual experiment was used 8 number of $Smartpeg^{TM}s$ and they had measured to ISQ values of before experiment and after experiment. Results: 1. The measured ISQ values did not change after fixation $Smartpeg^{TM}s$ for 400 times. 2. There was no significant changes in the measured ISQ values of 'attach-detach $Smartpeg^{TM}s'$ for 400 times. 3. The measured ISQ values did not change after the usual clinical sterilization methods. 4. The measured ISQ values did not change after sterilization in autoclave for 10 times. 5. It was impossible to exactly measure the critical temperature which is lost the magnetism of $Smartpeg^{TM}s$. But, the results was resulted to lost its magnetism in higher temperature than $150^{\circ}C$/10 minute. Conclusion: The measured ISQ values showed insignificant differences in case of no changes in the magnetism of the $Smartpeg^{TM}s$. It seems that the $Smartpeg^{TM}s$ can be used repeatedly in every measurement if the original magnetisms of the $Smartpeg^{TM}s$ can be recognized. There seems to be no significant changes in the measured ISQ values of 'attach-detach $Smartpeg^{TM}s'$ only if the screw pitches were unimpaired. The clinical sterilization methods seems acceptable because the result was resulted to lost its magnetism in higher temperature than $150^{\circ}C$/10minute.

• Korean Journal of Microbiology
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• v.47 no.1
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• pp.87-91
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• 2011

The aim of this study was to compare the performance of conventional culture and real-time PCR for detection of Cronobacter spp. in powdered foods. Infant formula, baby food and Misugaru inoculated with Cronobacter were enriched in distilled water as first enrichment step, followed by incubating in Enterobacteriaceae enrichment (EE) broth as second enrichment step. A loopful of enriched sample was streaked onto Druggan-Forsythe-Iversen agar, followed by incubating at $37^{\circ}C$ for 24 h. One milliliter of the enriched distilled water and EE broth were used in real-time PCR assay. No statistical differences were observed in the number of positive samples between culture method and real-time PCR (p>0.05) in all types of food samples. The number of positives of real-time PCR was higher in the first enrichment media (distilled water) than the second enrichment media (EE broth), though there was no significant difference (p>0.05). It appears that some components of the second enrichment broth, EE broth, inhibit the reaction of real-time PCR. These results show that real-time PCR using a single enrichment with distilled water could be useful as an effective screening method for detection of Cronobacter while saving much time and labor compared to conventional culture method.

• Journal of Bio-Environment Control
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• v.23 no.3
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• pp.250-255
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• 2014

Sterilization is an important factor in reprocessing of drained nutrient solution (RDN). To evaluate the suitability of ozone microbubble for RDN in protected horticulture and plant factory, strong fungicidal activity of ozone and microbubble were applied. This experiment was taken advantage of ozonated water (OW) and ozone gas (OG). The Fusarium oxysporum (FO), Phytophthora capsici (PC) and Colletotrichum gloeosporioides (CG) were treated with OW 0.5, 1.0 and 2.0 ppm and OG $3.0g{\cdot}h^{-1}$ for 0, 30, 60, 120 and 180 sec. Results of this experiment can be summed up as follows : In the OW, FO was sterilized by 0.5 ppm in 60 sec and PC was sterilized by 2.0 ppm in 30 sec. In the OG, FO and PC both of them were sterilized in 180 sec. However, CG was not sterilized using OW and OG. Overall, ozone microbubble showed possibility of sterilization in RDN. However, CG is required to more ozone concentration and processing time.

• Microbiology and Biotechnology Letters
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• v.17 no.5
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• pp.454-460
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• 1989

Antibiotics resistance genes both in natural bacterial isolates and the genetically cloned bacteria were comparatively studied for their transfer frequencies by the method of conjugation in several different water environments. The Kmr genes in both kinds of bacteria were transferred more frequently in autoclaved wastewater of laboratory environment than in natural river water, but in Luria Bertani (LB) broth medium under the laboratory conditions the transfer frequences of the genes were much higher than in the autoclaved wastewater. The transfer frequencies at 2$0^{\circ}C$ and 3$0^{\circ}C$ were not much different in any water environments. The Km$^{${\gamma}$}$ genes of the genetically cloned bacteria and the natural isolates were transferred at the same frequency both in natural river water and in the autoclaved wastewater of laboratory environment, but in LB broth under laboratory conditions the transfer frequencies were lowered by 10$^{-3}$ to 10$^{-4}$ in the genetically cloned cells than the natural isolates. When donors of different cloned cells were conjugated with recipient of a natural isolates, the Km$^{${\gamma}$}$ genes of different donor cells were transferred at the about same frequency, but the same donor of the cloned cell were conjugated with recipients of different natural isolates, the transfer of Km$^{${\gamma}$}$ gene of the cloned cell showed some differences of 101 to 102 in frequency.