• Journal of Marine Life Science
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• v.7 no.2
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• pp.61-73
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• 2022

Stable isotope analysis (SIA) is being used in various research fields including environmental science, ecology, biogeochemistry, forensics, and archeology. In this paper, for the purpose of enhancing applications and utilizations stable isotope analysis techniques to aquaculture research, we would like to introduce the background knowledge necessary to utilize stable isotope analysis techniques. In particular, with a focus on the approach using natural abundance, the principle of fractionation (change in isotope ratio) that occurs in the process of the integration of elements into biological tissues and how stable isotope ratios are determined by fractionation. This paper is intended to suggest whether SIA is used as a valuable tool in the fields of ecology and environmental science. With the understanding of the field of stable isotopes through this paper, various applications of stable isotope ratios are expected in fisheries science and aquaculture research in the future.