• Journal of the KSME
• /
• v.51 no.10
• /
• pp.44-48
• /
• 2011

최근 약 10년 동안 마이크로 유체 시스템은 다양한 세포연구에 광범위하게 활용되며 생물학과 의학 분야에 새로운 전기를 가져왔다. 마이크로 유체 시스템 내의 유동현상을 설계하고 연구에 적용하는 일은 기계공학도가 중추적인 역할을 해야 하는 분야이다. 유동장 내에서 관찰되는 세포의 반응은 매우 흥미로울 뿐 아니라 학술적으로 막대한 파급력있는 결과를 가져오기도 한다. 이 글을 통해 전통적인 접근법으로는 성취하기 힘들었던 세포연구들이 마이크로 유체 시스템을 이용하여 어떻게 효과적으로 수행되는지 소개하고자 한다.

• Journal of the Microelectronics and Packaging Society
• /
• v.29 no.4
• /
• pp.55-61
• /
• 2022

There is a major interest in diagnostic technology for multiple cancers worldwide. In order to reduce the difficulty of cancer diagnosis, a liquid biopsy technology based on a microfluidic device using trace amounts of biofluids such as blood is being studied. And optical biosensing, which measures the concentration of analytes through fluorescence imaging using biofluids, requires various strategies to improve sensitivity, and specialists and equipment are needed to carry out these strategies. This leads to an increase in diagnostic and production costs, and it is necessary to develop a technology to solve this problem. In this paper, we design and propose a fluorescent multi-cancer diagnostic sensing platform structure that implements passive self-separation technology and molecular recognition activation functions by fluid mixing, only with the geometry and microfluidic phenomena of microchannels based on self-driven flow by capillary force. In order to check the parameters affecting the performance of the plasma separation part of the designed sensor, the hydrodynamic diameter of the channel and the viscosity of the fluid were set as variables to confirm the formation of plasma separation flow through simulation. And finally, we propose an optimal sensor platform structure.

• Proceedings of the Korean Institute of Surface Engineering Conference
• /
• 2017.05a
• /
• pp.109-109
• /
• 2017

알지네이트 하이드로 젤은 해조류에서 추출되는 천연 고분자인 알지네이트가 칼슘 또는 마그네슘 양이온과 이온가교(Ioninc cross linking)를 형성할 때 알지네이트의 고분자 구조가 칼슘, 마그네슘 양이온을 감싸면서 형성되는 고분자이다. 알지네이트 하이드로 젤은 높은 생체적합성(Biocompatibility)으로 인해 세포 재생을 위한 조직공학 및 재생의학, 약물전달 등의 제약 관련 분야에 광범위하게 적용될 수 있는 물질로 많은 연구가 이루어지고 있다. 본 연구에서는 마이크로 플루이딕 칩을 이용하여 알지네이트 튜브를 제조하였다. 먼저 유동 포커싱 방식(flow focussing)을 유도할 수 있는 PDMS(Polydimethylsiloxane) 마이크로 플루이딕 칩을 제조하였다. 마이크로 플루이딕 칩은 CNC(Computer Numeric Control) milling machine을 이용한 template를 만들고 NOA mold를 이용하여 최종 PDMS 칩을 제작하였다. 튜브를 만들기 위한 마이크로 채널은 내부 채널 ($200{\times}200um$), 중간 채널 ($200{\times}200um$) 및 외부 채널 ($200{\times}200um$)로 구성되며 내부, 중간, 외부의 유체가 합류하는 수집채널은 폭 500 um, 깊이 200 um로 구성되었다. 운반체로는 5%의 acetic acid를 함유한 mineral oil를 이용하였으며 내부의 core flow는 $H_2O$로 하였다. 중간 유체인 2% 알지네이트 프리폴리머는 칼슘 이온의 존재 하에서 젤화 과정이 매우 빠르기 때문에 마이크로 채널 내부에서의 반응을 제어하고 막힘을 방지하기 위해 수용성 복합 칼슘-에틸렌 디아민 테트라 아세트산 (EDTA)을 사용하였다. 본 마이크로 플루이딕 칩에 각각의 유체를 이동시켰을 때, 운반체인 oil phase의 수소이온은 중간 유체인 알지네이트 프리폴리머와의 계면을 통해 확산되어 Ca-EDTA 복합체로부터 칼슘 양이온의 방출을 유발하게 된다. 방출된 칼슘 양이온은 알지네이트 고분자와의 이온 가교를 통해 알지네이트 하이드로 젤을 형성하여, 각 유체의 flow에 따라 알지네이트 튜브를 쉽고 빠르게 제조 가능하였다. 본 연구에서 제조된 알지네이트 튜브는 인체 내 장기간 약물 전달을 위한 나노섬유로 활용하거나 인공혈관을 구성하는 extracellular matrix로 활용될 잠재력을 가지고 있어 추후 활발한 연구개발이 진행될 예정이다.

• Electrical & Electronic Materials
• /
• v.36 no.3
• /
• pp.4-12
• /
• 2023

• Journal of computational fluids engineering
• /
• v.8 no.2
• /
• pp.16-23
• /
• 2003

A numerical study of the mixing of two fluids(pure water and a solution of glycerol in water) in a microchannel was carried out. By varying the glycerol content of the glycerol/water solution, the variation in mixing behavior with changes in the difference of the properties of the two fluids(e.g., viscosity, density, diffusivity) was investigated. The mixing phenomena were tested for three micromixers: a square mixer, a three-dimensional serpentine mixer, and a staggered herringbone mixer. The governing equations of continuity, momentum and solute mass fraction were solved numerically. To evaluate mixing performance, a criterion index of mixing of mixing uniformity was proposed. In the systems considered, the Reynolds numbers based on averaged properties were 1 and 10. For low Reynolds number (Re = 1), the mixing performance varied inversely with mass fraction of glycerol due to the dominance of molecular diffusion. The mixing performance by diffusion deteriorated due to a significant reduction in the residence time of the fluid inside the mixers.

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
• /
• v.34 no.2
• /
• pp.33-38
• /
• 2005

열저장 및 열수송 측면에서 장점을 가진 기능성 유체인 마이크로캡슐 잠열재 슬러리의 특징 및 국내의 이용 기술 현황을 소개하고자 한다.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.46 no.4
• /
• pp.15-20
• /
• 2009

In a detection system based on laser light scattering, focusing an excitation laser beam into a focal point of a channel in a microfluidic chip is important for obtaining the highest excitation intensity, and consequently for obtaining a laser light scattering signal using a photodetector with a high efficiency. In this paper, we present a polydimethylsiloxane (PDMS) microfluidic chip consisting of an integrated PDMS microlens for cell detection based on laser light scattering. We fabricated PDMS microlens for optical detection system by simply putting down on PDMS chips. The PDMS microlens was fabricated by photoresist reflow and replica molding. This fabrication technique is simple and has an excellent property in terms of the microlens and a high-dimensional accuracy. The PDMS microlens integrated on the PDMS microfluidic chip has been verified to improve the laser intensity, and accordingly, the signal-to-noise ratio and sensitivity of laser light scattering detection for red blood cells(RBCs)

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.23 no.1
• /
• pp.23-31
• /
• 2011

An analysis has been conducted of the flow characteristics and pumping performance of a thermopneumatic micropump with electrically conducting fluid. In the present study, considered is a thermopneumatic micropump for electrically conducting fluids with electromagnetic resistance alternately exerted at the inlet and outlet by alternately applied magnetic fields. A model of Prescribed Deformation is used for the motion of the membrane. Here, the pumping performance of the micropump and flow characteristics of the electrically conducting fluid are investigated in the range of Hartmann number less than 30. The current numerical study shows that the net flow rate through the micropump is almost proportional to the strength of the applied magnetic field.

• Journal of computational fluids engineering
• /
• v.11 no.4 s.35
• /
• pp.101-106
• /
• 2006

In the microfluidic devices the most important thing is mixing efficiency ol various fluids. In this study a newly designed miler is proposed to enhance the mixing effect with the purpose to apply it to microchannel mixing in a short future. This design is composed of a channel with cross baffles periodically arranged on the both bottom and top surfaces ol the channel. To obtain the yow patterns, the numerical computation was performed by using a commercial code, ANSYS CFX 10.0. To evaluate the mixing performance, we computed Lyapunov exponent and obtained Poincare sections. it was shown that our design provides the excellent mixing effect.

• Journal of computational fluids engineering
• /
• v.14 no.4
• /
• pp.86-92
• /
• 2009

Nowadays, the development of microfluidic chip [i.e. biochip, micro-total analysis system ($\mu$-TAS) and LOC (lab-on-a-chip)] becomes more active, and the microchannels to deliver fluid by pressure or electroosmotic forces tend to be more complex like electronic circuits or networks. For a simple network of channels, we may calculate the pressure and the flow rate easily by using suitable formula. However, for complex network it is not handy to obtain such information with that simple way. For this reason, Graphic User Interface (GUI) program which can rapidly give required information should be necessary for microchip designers. In this paper, we present a GUI program developed in our laboratory and the simple theoretical formula used in the program. We applied our program to simple case and could get results compared well with other numerical results. Further, we applied our program to several complex cases and obtained reasonable results.