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Rice Cultivar Breeding for Rice Flour with Low Amylose by Tissue Culture (저아밀로스 특성 및 쌀가루 적합 벼 품종 육성)

  • Park, Jae-Ryoung;Kim, Eun-Gyeong;Jang, Yoon-Hee;Kim, Kyung-Min
    • Journal of Life Science
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    • v.32 no.9
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    • pp.698-705
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    • 2022
  • Rice consumption in Korea is continuously decreasing; therefore, methods for reducing rice stocks are now being proposed. Increasing the consumption of rice and reduce these stocks will require that the rice processing industry be developed beyond simply using cooked rice as food. The Korean people's eating habits have also diversified recently, and the consumption of bread is increasing. Therefore, breeding a cultivar for rice flour is now necessary. Here, a line suitable for processing into rice flour was cultivated by deriving a mutant line by tissue culture of Ilpum. Mutant populations were investigated for major agricultural traits in the field, and grain characteristics and endosperm characteristics were investigated after harvest. Among the lines, line 29111 had similar agricultural characteristics to the background Ilpum, and excellent grain quality, and was selected for further analysis. The physicochemical properties that determine taste were similar or improved. However, unlike Ilpum, 29111 had a non-uniform starch structure and a characteristic powdery endosperm because large and male starch grains are evenly distributed. Therefore, 29111 was viewed as a breeding line suitable for processing and for excellent agricultural characteristics and taste. The added value can be increased by diversifying the uses of rice. The taste was improved and a method for breeding cultivars with grain characteristics for glutinous rice that would satisfy both producers and consumers was presented.

Variation of Protein Content and Amino Acid Composition in Perilla Germplasm (들깨 유전자원의 단백질함량과 아미노산조성)

  • Lee, Jung-Il;Bang, Jin-Ki;Lee, Bong-Ho;Kim, Kwnag-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.5
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    • pp.449-463
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    • 1990
  • To obtain the basic informations on quality improvement, seed protein and amino acid composition were analyzed in 460 strains of perilla germplasm. Among the tested strains, total protein content ranged from 17.9% to 28.1 % with the 23.6% of varietal means. Form the experiment, Namji, Sandong, and Eunjin were selected as high protein strains of which content was as high as 28.1%. In protein content, collected strains from Jeonnam province showed highest, and was not significantly different by maturity, but this characteristics showed differences by seed coat color and 1,000 seed weight. The significantly negative correlation was observed between protein content and seed setting ratio. However it was observed that significant and high positive correlation between protein and oil content. A calibration for an Infra-Alyzer 450 using log reflectance readings at 2208, 1982, 1940 and 1722nm could be used without adjustment for the measurment of the protein content in perilla with a standard deviation of differences against micro-kjeldahl of 0.27%. The amino acid composition of perilla was similar to the other oilseed crops, and showed a relatively high lysine and methionine content. Further, amino acid composition of perilla seed was exellently characterized with bal ance and higher than FAO recommendation. Major amino acids were indentified as a glutamic acid and arginine in perilla seed protein.

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Grain Quality Characteristics for Brewing in Rice (벼품종의 양조적성관련 미질특성)

  • Ha, Ki-Yong;Lee, Jae-Shin;Kwon, Eui-Kyeon;Lee, Jae-Kil;Lee, Seon-Yong;Park, Nou-Poung;Park, Keun-Yong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.1
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    • pp.38-44
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    • 1994
  • The study was conducted to investigate some grain quality characters related with brewing fittness for rice wine by adopting eight rice varieties as materials. Six japonica rice varieties except two Tongil-type rice had short and round grain of 1.80 or lower grain length/width ratio. 1000-grain weight of brown rice was 21.7~29.5g of significant difference among varieties. The white-center of rice grain was most severe in Hidahomare and Iri 402. Among tested varieties, Seomjinbyeo, Iri 402, Dongjinbyeo and Hidahomare had 50% or higher head rice ratio, while the others had relatively worse milling properties resulted from higher ratio of notched-belly and broken grain. Chemical components and total acid and amino acid content of refined rice wine was relatively good in every variety. Liquifying and saccharificating power was higher and koji conditon was also good in the varieties of high head rice ratio. Seomjinbyeo, Dongjinbyeo and Iri 402 was preferred in sensory test of refined wine and was recognized as superior adaptable rice varieties for brewing rice wine.

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Introduction, Development, and Characterization of Supernodulating Soybean Mutant. 1. Mutagenesis of Soybean and Selection of Supernodulating Soybean Mutant (다량 뿌리혹 형성 콩 계통의 도입 개발 및 생육특성구명 1. 돌연변이유기에 의한 콩 초다뿌리혹형성 계통선발)

  • Lee, Hong-Suk;Chae, Young-Am;Park, Eui-Ho;Kim, Yong-Wook;Yun, Kwang-Il;Lee, Suk-Ha
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.2
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    • pp.247-253
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    • 1997
  • Development of soybean cultivars with great nodulation and high nitrogen fixation activity, derived mostly from mutagenesis, may decrease inputs of chemical fertilizer nitrogen into the soil-plant system. Soybean seeds (cv. Jangyupkong, Hwanggeumkong, and Geomjungkong 1) were treated with three different levels of EMS (ethyl methanesulfonate) concentration(30, 50, and 70mM). Increasing the doses of EMS resulted in decreased field emergence rate of seeds, whereas it did not increase M$_2$ mutation frequencies. This indicated that the most efficient concentration of EMS was 30mM for generating mutants. Extensive mutagenesis of Sinpaldalkong 2 with 30mM EMS was undertaken to isolate soybean mutants with greater nodulation. Approximately 8, 200 M$_2$ families were screened for greater nodulation on 5 mM nitrate after inoculation with Bradyrhizobium japonicum strain YCK213-KFCC-10728. Mutant SS-2 nodulated more than the wild type. Comparison of supernodulation between SS-2 and two nts mutants(nts 1007 and nts 1116) revealed that SS-2 showed the supernodulation character at an earlier growth stage than the two nts mutants. Further studies should be needed to characterize the difference in timing of nodulation between SS-2 and nts mutants.

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A study on environmental flow release through flood control reservoir (담양·화순 홍수조절지 환경대응용수 활용방안 연구)

  • Dong Gue Choe;Jong Duk Park;Dong Jin Kim;Seon Woong Ryu;Soo Jin Lee
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.371-371
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    • 2023
  • 담양 및 화순 홍수조절지는 홍수 시 본류에 계획방류량만 흘러가도록 제수문을 운영하고, 계획방류량을 초과하는 홍수량은 하도 및 조절지 내에 저류하여 홍수를 지체시킨 후 저류된 홍수량은 첨두홍수가 지나간 이후 방류하는 방법으로 영산강 유역의 홍수를 방어하기 위해 '12년 건설되었다. 그러나 홍수조절지는 200년 빈도의 홍수량을 저감 시키도록 설계됨에 따라 준공 이후 10년간 '20년 8월 홍수 등 3회만 홍수방어용으로 활용되었다. 영산강은 오염부하량 대비 유출량 부족 등으로 수질관리에 취약하고 하천유지용수를 공급하는 다목적댐이 없는 등 수자원 확보가 어려우므로, 비홍수기 홍수조절지에 환경대응용수를 담수 및 방류하여 영산강의 유량확보 및 수질개선 등 활용 다변화 방안을 모색하고자 한다. 이와 관련하여 '16년 및 '19년에 시범담수를 실시하였으나, 담수 수질 관리의 어려움, 방류 전후 강우 등 제한적 여건으로 인해 하류 유량증가 및 수질 개선 효과의 확인은 어려웠으며, 제방 물비침 등을 발견하여 시설 안정성 확보가 필요한 것으로 확인되었다. '21년 안정성 확보를 위한 보강 그라우팅을 시행했으며, '19년부터 '21년 2월까지 「홍수조절지를 활용한 영산강 수질개선 방안」연구용역을 실시하여, 비홍수기 활용방안을 수립하였다. '21년 4/4분기 댐과 보 등의 연계운영협의회에 '시범담수 계획' 안건 상정 및 의결을 거쳤고, '22년 3월 25~26일까지 담양 74mm, 화순 61mm 비가 내려 담양 63만m3, 화순 151만m3를 담수하고 18~19일간 저류 후 담양은 평균 3.0m3/초로 25시간 동안, 화순은 평균 5.0m3/초로 32시간 동안 하류로 방류하였다. 방류 후 담양·화순 모두 가까운 하류 수위국 수위 상승을 확인했으며, 화순의 경우 하류 수질에 일부 영향이 있음을 확인하였다. 다만, 초기유출수 배제 등을 고려하지 않아 담수의 수질이 본류보다 좋지 않았으며, 이에 따라 수질 영향의 인과관계 확인이 어려웠다. 기존 연구용역에서 제시한 비홍수기 활용방안의 검증·보완을 위한 연구용역이 '22.5월부터 '23.12월까지 진행될 예정이며, 초기유출수 배제를 위한 조건 재수립, 수질 모니터링지점 확대 등 보완된 '시범담수 계획'을 '23.3월 댐과 보 등의 연계운영협의회에 보고하였다. 연말까지 연구용역과 연계한 시범담수·방류 시행 및 모니터링·효과분석을 통해 비홍수기 홍수조절지 최적운영방안을 마련할 계획이다.

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Effect of Seeding Times on Growth and Yield of Rice in Puddled Drill Seeding at Southern Plain Area (남부평야지 벼 무논 골뿌림 재배시 파종시기가 생육 및 수량에 미치는 영향)

  • Kim, Sang-Su;Baek, Nam-Hyun;Seok, Soon-Jong;Lee, Seon-Yong;Cho, Dong-Sam
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.2
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    • pp.212-220
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    • 1995
  • The optimum seeding date and the suitable seeding period for puddled drill seeding of rice at southern plain area were determined by using Sinunbongbyeo (early maturing variety), Hwaseongbyeo(medium maturing var.) and Dongjinbyeo (late medium maturing var.) at National Honam Agricultural Experiment Station from 1993 to 1994, Seedling stand was decreased significantly at seeding on April 25 in all varieties, Threshold seed-ing dates for stable ripening was estimated as June 10 in Sinunbongbyeo, May 31 in Hwaseongbyeo and May 25 in Dongjinbyeo, The 1,000 grain weight and the ripened grain rate were highest at seed-ing on May 10 or May 25 in all varieties, and decreased significantly at seeding on June 25 in Sinunbongbyeo and seeding after June 10 in Hwaseongbyeo and Dongjinbyeo, The optimum average air temperature for 40 days after heading was 22.6$^{\circ}C$ for highest ripening rate and 22.0$^{\circ}C$ for heaviest 1,000 grain weight. The optimum seeding date for highest yield was May 9 in Dongjinbyeo, May 13 in Hwaseongbyo and May 15 in Sinunbongbyeo.

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Settlement Prediction Accuracy Analysis of Weighted Nonlinear Regression Hyperbolic Method According to the Weighting Method (가중치 부여 방법에 따른 가중 비선형 회귀 쌍곡선법의 침하 예측 정확도 분석)

  • Kwak, Tae-Young ;Woo, Sang-Inn;Hong, Seongho ;Lee, Ju-Hyung;Baek, Sung-Ha
    • Journal of the Korean Geotechnical Society
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    • v.39 no.4
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    • pp.45-54
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    • 2023
  • The settlement prediction during the design phase is primarily conducted using theoretical methods. However, measurement-based settlement prediction methods that predict future settlements based on measured settlement data over time are primarily used during construction due to accuracy issues. Among these methods, the hyperbolic method is commonly used. However, the existing hyperbolic method has accuracy issues and statistical limitations. Therefore, a weighted nonlinear regression hyperbolic method has been proposed. In this study, two weighting methods were applied to the weighted nonlinear regression hyperbolic method to compare and analyze the accuracy of settlement prediction. Measured settlement plate data from two sites located in Busan New Port were used. The settlement of the remaining sections was predicted by setting the regression analysis section to 30%, 50%, and 70% of the total data. Thus, regardless of the weight assignment method, the settlement prediction based on the hyperbolic method demonstrated a remarkable increase in accuracy as the regression analysis section increased. The weighted nonlinear regression hyperbolic method predicted settlement more accurately than the existing linear regression hyperbolic method. In particular, despite a smaller regression analysis section, the weighted nonlinear regression hyperbolic method showed higher settlement prediction performance than the existing linear regression hyperbolic method. Thus, it was confirmed that the weighted nonlinear regression hyperbolic method could predict settlement much faster and more accurately.

Evaluation of the Effect of Waveform Micropiles on Reinforcement of Foundation Structures Through Field Load Tests (현장 재하시험을 통한 파형 마이크로파일의 기초보강 효과 분석)

  • Baek, Sung-Ha;Han, Jin-Tae;Kim, Seok-Jung;Kim, Joonyoung
    • Journal of the Korean Geotechnical Society
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    • v.39 no.3
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    • pp.29-40
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    • 2023
  • In this study, we investigated the reinforcing effects of waveform micropiles in a stratigraphic setting comprising buried soil, weathered soil, and weathered rock. We conducted a series of field load tests and determined that waveform micropiles exhibited sufficient bearing capacity through frictional resistance in the soil layer and demonstrated favorable constructability in conditions with deep bedrock layers. Moreover, the vertical stiffness of waveform micropiles was approximately 2.2 times higher than that of conventional micropiles when subjected to the same design load. Pile group load tests comprising conventional and waveform micropiles showed that micropiles with higher stiffness carried a greater proportion of the load. Although there was no significant difference in the bearing capacity between conventional and waveform micropiles under the same design load, waveform micropiles with higher stiffness showed a load-carrying capacity 1.7 to 3.2 times greater than that of conventional micropiles. These findings suggest that waveform micropiles can be effectively used for foundation reinforcement and reduce the risk of foundation failure when increased loads due to modifications such as expansion remodeling are expected.

Microstructure Development during Sintering of $Nb_2O_5$-doped $UO_2$ pellets under $H_2$ and $CO_2$ atmospheres ($Nb_2O_5$ 첨가 $UO_2$ pellet의 수소 분위기와 이산화탄소 분위기 소결 중 미세조직의 형성에 대한 연구)

  • Song, K.W.;Kim, S.H.;Kim, B.G.;Lee, Y.W.;Yang, M.S.;Park, H.S.
    • Nuclear Engineering and Technology
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    • v.26 no.4
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    • pp.484-492
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    • 1994
  • Microstructures of Nb$_2$O$_{5}$-doped UO$_2$ pellets have been investigated during sintering under H$_2$ and $CO_2$ atmospheres. Pellets are sintered at 1$700^{\circ}C$ in H$_2$ atmosphere and at 130$0^{\circ}C$ in $CO_2$ atmosphere for 1 to 41 hr. The addison of Nb$_2$O$_{5}$ causes the formation of large pores, which shrink to some extent in H$_2$ atmosphere but very little in $CO_2$. Fine pores in the Nb$_2$O$_{5}$-doped UO$_2$ pellet are almost annihilated when sintered under H$_2$ atmosphere but little changed under $CO_2$ atmosphere. The increase in grain size due to Nb$_2$O$_{5}$ addition is much larger in H$_2$ atmosphere than in $CO_2$. Thus the enhancement of uranium diffusion in UO$_2$ due to the Nb$_2$O$_{5}$ addition is thought to be more significant in H$_2$ atmosphere. Microstructures of Nb$_2$O$_{5}$-doped UO$_2$ pellets sintered in H$_2$ atmosphere are discussed from the viewpoint of in-reactor performance. Possible defects formation due to Nb$_2$O$_{5}$ addition is discussed to explain the enhancement of uranium diffusion in H$_2$ and $CO_2$ atmospheres.> atmospheres.

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Cannabidiol Inhibits Lipogenesis by Regulating Akt/AMPK-SREBP-1 Pathway in Sebocytes (피지세포에서 Akt/AMPK-SREBP-1 경로를 통한 CBD의 피지 합성 억제 효능)

  • Yoon Gyung Kwon;Ji Young Yoon;Hanon Lee;Dong Hyo Kim;Jun Hyo Lee;Diane M Thiboutot;Dae Hun Suh;Byoung Jun Park
    • Journal of Life Science
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    • v.33 no.4
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    • pp.343-348
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    • 2023
  • Acne is one of the most common skin diseases, mainly occurring in adolescence. The pathophysiology of acne involves not only hormonal, genetic and environmental factors, but also other factors including hyperseborrhea, inflammation, over-keratinization of follicular keratinocytes and overgrowth of Cutibacterium acnes (C. acnes). Cannabidiol (CBD) is known to relieve pain, stress and inflammation. Moreover, cannabis extracts containing CBD have been reported to be effective in treating acne. However, the therapeutic effect of CBD on acne remains unclear. Therefore, this study aimed to investigate the effect and mechanism of CBD on lipogenesis in SEB-1 sebocytes. We treated sebocytes with CBD and found that it not only inhibited lipid synthesis, but also inhibited cell proliferation by inducing apoptosis. We then demonstrated that sterol response element-binding protein-1 (SREBP-1) mediates the inhibitory effect of CBD on lipogenesis. Furthermore, Akt and adenosine monophosphate-activated protein kinase (AMPK), upstream regulators of SREBP-1, were regulated by CBD treatment. Taken together, our studies demonstrate that CBD inhibits adipogenesis by regulating the Akt/AMPK-SREBP-1 signaling pathway, providing potential for use as a therapeutic agent for acne. Further research is needed to confirm the effect of CBD on inflammation caused by hyperkeratosis, which will increase the possibility of using CBD for acne treatment.