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Extraction Conditions and Quality Stability of Carotenoprotein from Krill Processing Waste by Proteolytic Enzymes (크릴 가공폐기물을 이용한 Carotenoprotein의 추출조건 및 품질안정성에 관한 연구)

  • Kim Se-Kwon;KiM Yong-Tae;KWAK Dong-Chae;CHO Duck-Jae;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.1
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    • pp.40-50
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    • 1990
  • The purpose of this paper is to develop a colorant from krill, Euphausia superba, process wastes for use in food products. Carotenoproteins were extracted from preboiled krill processing offal(PKPO) and raw frozen krill processing offal(RKPO) with the aid of proteolytic enzymes. The long-term stability of the astaxanthin associated with the carotenoprotein by the addition of pretense inhibitor and antioxidant to the product were also investigated. Total astaxanthin contents of PKPO and RKPO were $35.1mg\%,\;22.1mg\%$ and those in carotenoproteins were $98.6mg\%,\;61.9mg\%$, respectively. The chitin contents of PKPO and RKPO were $6.9\%,\;4.5\%$, however, those of carotenoproteins were not determined. When $0.5\%$ trypsin was added to the extraction medium containing 0.5M $Na_3EDTA$ at $4^{\circ}C,\;74\%$ of astaxanthin and $83\%$ of the protein of PKPO were recovered as carotenoprotein in 24hrs. The amino acid profile in carotenoprotein was mainly composed of glutamic acid, methionine, aspartic acid and isoleurine. Their contents amounted to about 40% of the total amino acids, followed by alanine, phenylalanine, Iysine, leucine, threonine and tyrosine in that order, with a small amount of cysteine and tryptophan. The levels of essential amino acids were high as much as $38.3\%\~43.6\%$ of the total amino acids. The maximum observance of the carotenoid fraction from krill processing offal and from carotenoprotein was 469nm in petroleum ether. The separated components of carotenoprotein by TLC had Rfs $0.20\~0.23\;0.56\~0.60$ and $0.88\~0.91$. The carotenoids were comprised of astaxanthin, astaxanthin monoester and asthaxanthin diester in $25\~30\%\;,35\~40\%$and $40\~45\%$, respectively. The loss of carotenoids in the carotenoprotein can be prevented by the addition of pro-tease inhibitor(trasylol) and antioxidant(BHT) below $4^{\circ}C$.

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Studies on Sanitary Conditions in a Special Grade Chicken Processing Plant in Korea I. Storage Period of Packed Chicken Meat in relation to the Bacterial Contamination of Each Processing Part (특급도계장의 위생실태에 관한 연구 I. 작업부분별 세균오염도 측정)

  • 오경록
    • Korean Journal of Poultry Science
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    • v.13 no.1
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    • pp.41-48
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    • 1986
  • In order to investigate the storage period of packed chicken meat in relation to the bacterial contamination of each processing part, a special grade poultry processing plant located at Dongdoochun-City, Gyeonggi-Do, was surveyed mainly in point of sanitary conditions. The results are summarized as follows. 1. processing room near reception and packing room next to shipping area showed the highest bacterial counts in air. 2. Equipments and instruments for slaughtering also showed a high degree of bacterial contamination even before the operation. This finding suggested that the possibility of the bacterial contamination of carcasses through equipments and instruments would be high, if the continuous sanitary control measures are not properly taken. 3. The bacterial count of scalding water was 3${\times}$10$\^$7/ for standard plate count and 4${\times}$10$\^$5/ for most probable number of coliform bacteria at 1 hour after the start of operation. These values were maintained until the completion of processing. 4. At the ending of chilling process, the bacterial counts of chilling water were 3.3 ${\times}$10$^4$ and 3.0${\times}$10$^3$for standard plate count, when the volume of water used per bird were 21 and 51, respectively. Bacterial contamination was further decreased about 10$\^$-3/-10$\^$-4/ times as the effective chlorine concentration in chilling water was maintained about 50 ppm. 5. An average standard plate count of the residual water In abdominal cavity of carcasses was 3.9${\times}$10$^4$immediate after chilling, however, it increased to 4.7${\times}$10$\^$7/ when the carcasses were packed and stored for 10 days at 4$^{\circ}C$. off-odour development was observed as the bacterial count was not less than 10$\^$6//$m\ell$ of the residual water of the packed carcasses. Data indicated that if the standard plate count of the residual water in abdominal cavity of carcasses was not less than l0$^4$immediate after chilling, the packed chicken meat would not be stored longer than 5 days at 4$^{\circ}C$.

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Influence of lime and phosphorus application on nutrient uptake by corn in newly reclaimed acidic soils. -II. With special reference to soil texture and distribution of zinc in leaves and stem (산성신개간토양(酸性新開墾土壤)에서 석회(石灰) 및 인산(燐酸)이 옥수수의 양분흡수(養分吸收)에 미치는 영향(影響) -II. 토성(土性)과 아연(亞鉛)의 흡수(吸收) 및 부위별(部位別) 분포(分布))

  • Kim, Young-Koo;Hong, Chong-Woon;Oh, Yong-Taeg
    • Korean Journal of Soil Science and Fertilizer
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    • v.9 no.2
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    • pp.77-81
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    • 1976
  • On a newly reclaimed acidic soil, investigation was made to find out the influence of lime and phosphorus application by a large quantity on the uptake of zinc by corn and its distribution in leaves and stem, under a factorial combination with two levels of zinc, lime, and phosphorus with three kinds of soil texture. The results are summarized as following, 1. The concentration of zinc in stem reflected the zinc uptake status of corn better than the leaves. The concentration of zinc in stem responded clearly to the treatments of lime and zinc, while those of leaves tended to be constant regardless of the application of lime and zinc. 2. The zinc uptake was not improved by compost application though it increased the yield of corn significantly. 3. The application of lime for neutralization of soil with low level of phosphorus application affected the yield of dry matter differently among different soils, slight increase on sandy loam soil, slight decrease on loam soil, and significant decrease on clay loam soil. The yield decrease on clay soil is considered to be due to the decreased availability of applied phosphorus owing to the large amount of lime applied.

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Studies on the Absorption Capacity of Phosphorus of Korean Top-soils (우리나라 표층토(表層土)의 인산흡수력(燐酸吸收力)에 관(關)한 연구(硏究))

  • Park, C.S.;Han, K.H.;Lim, S.K.;Lee, J.H.
    • Korean Journal of Soil Science and Fertilizer
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    • v.2 no.1
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    • pp.1-13
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    • 1969
  • A total of 129 surface(upto 30 cm depth) soil samples were selected from the profile samples collected during reconnaissance soil survey in 1967, for the determination of phosphorus absorption co-efficient. The distribution range for each soil association has been established. The physicochemical factors affecting the phosphorus absorption coefficient have also been examined. The following general conclusions can be drown: 1. In general, the phosphorus absorption coefficient of the soil association of presently arable land are lower than the soils which are not in cultivation. 2. The higher the cation exchange capacity of soils, the higher is the phosphorus absorption coefficient. The factors governing phosphorus absorption coefficient in various soil associations are as follows: Parent Material Soil Association Governing Factor Fluvio marine Low Humic Gley Fluvio marine Alluvial Complex Narrow valley Siliceo mafic materials Red-yellow podzolic Redish Siliceo mafic materials Brown Lateritic Clay content Siliceous crystalline materials Lithosols C.E.C. & Clay content Alluvium Low Humic Alluvium Gley Alluvial Organic matter Siliceous crystalline materials Red-Yellow Podzolic Organic matter and clay content 4. The relation between phosphorus absorption coefficient determined by $(NH_4)_2HPO_4(y)$ and by the P 700 ppm $NaH_2PO_4(x)$ is $Y=2.716X+37(r=0.96^{**})$ which shows highly significant positive correlation and linear regression.

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Construction and Tests of the Vacuum Pumping System for KSTAR Current Feeder System (KSTAR 전류전송계통 진공배기계 구축 및 시운전)

  • Woo, I.S.;Song, N.H.;Lee, Y.J.;Kwag, S.W.;Bang, E.N.;Lee, K.S.;Kim, J.S.;Jang, Y.B.;Park, H.T.;Hong, Jae-Sik;Park, Y.M.;Kim, Y.S.;Choi, C.H.
    • Journal of the Korean Vacuum Society
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    • v.16 no.6
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    • pp.483-488
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    • 2007
  • Current feeder system (CFS) for Korea superconducting tokamak advanced research(KSTAR) project plays a role to interconnect magnet power supply (MPS) and superconducting (SC) magnets through the normal bus-bar at the room temperature(300 K) environment and the SC bus-line at the low temperature (4.5 K) environment. It is divided by two systems, i.e., toroidal field system which operates at 35 kA DC currents and poloidal field system wherein 20$\sim$26 kA pulsed currents are applied during 350 s transient time. Aside from the vacuum system of main cryostat, an independent vacuum system was constructed for the CFS in which a roughing system is consisted by a rotary and a mechanical booster pump and a high vacuum system is developed by four cryo-pumps with one dry pump as a backing pump. A self interlock and its control system, and a supervisory interlock and its control system are also established for the operational reliability as well. The entire CFS was completely tested including the reliability of local/supervisory control/interlock, helium gas leakage, vacuum pressure, and so on.

Geochemical exploration for REE occurrence in Nghe An Area within Northern Vietnam (베트남 북부 네안 희토류 산출지의 지구화학탐사)

  • Heo, Chul-Ho;Chung, Ho Tien;Lee, Jae-Ho
    • Economic and Environmental Geology
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    • v.45 no.6
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    • pp.599-622
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    • 2012
  • The phase I soil geochemical exploration was carried out targeting around Chau Binh area far from about 14 km with southeastern direction from Quy Chau within Nghe An province. The interval of sampling are horizontal 300 m with 14 line and longitudinal 500 m with 15 line, resulting in 194 soil samples. Based on the result of the phase I soil geochemical exploration, the phase II detailed pitting survey was carried out targeting the grid point with high TREO content, resulting in 56 soil samples within 7 pits. The geology of survey area are consisted of Ban Chieng biotite granite complex and Dai Loc gneissic granite complex intruding Bu Khang formation comprising of schist, gneiss and limestone. Main mineralization in the study area have the characteristics of occurrence with tin, ruby and REE-bearing monazite(about 300 g/t) and xenotime(about 10 g/t) to be thought as occurring at the alteration zone of granite complex. In order to elucidate the source rock of monazite and xenotime confirmed from heavy sand, soil geochemical exploration was carried out. As a analysis result with ICP-MS on the soil samples from the phase I soil geochemical exploration, total REE oxide content of background amount to about 2 times of crustal abundance, enriching the heavy rare earth(about 2 times) and light rare earth(about 1.84 times). As a analysis result with ICP-MS on the soil samples from the phase II soil detailed pit survey, we identified outcrop considering as economic ore body at the grid point 4-7 pit with N40W attitude. As a synthetic consideration on the phase I soil geochemical exploration and phase II detailed pit survey, we tentatively designated areas considering as the extension of economic ore body with REE anomaly. In the near future, we have the plan to carry out the geophysical exploration and test drilling targeting the interval anticipated to the economic ore body.

A Survey on Risk Factors Related to Experience Rate of Low Back Pain in High School Students (일부지역 고등학생들의 요통경험율과 관련 요인 -안동시를 중심으로-)

  • Kim, Shun-Yeop;Yi, Seung-Ju;Park, Sang-Rae;Jang, Young-Ho;Cha, Sang-Eun;Kim, Ji-Sook
    • Journal of Korean Physical Therapy Science
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    • v.2 no.3
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    • pp.653-666
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    • 1995
  • This study was conducted to investigate factors related to experience rate of low back pain(LBP) in the high school students, a questionnaire survey was carried out for 778 high school students in Andong city Kyungsang Pook province from 28th June to 10th July, 1993. The results were as follows: The experience rate of LBP for 778 high school students who were interviewed was 27.1 %. Unknown(47.4 %) was the highest in the cause of LBP. The experience rate of a academical high school students (57.8 %) was higher than the technical high school students(42.7 %) in association according to LBP and school division(P = 0. 001). The experience rate of 18 years old above students (60.2 %) was higher than 17 years old below school students(39.8 %%) in association between LBP and age(P = 0.031). The experience rate of students who go to bed at the hot floored bed (80.1 %) was higher than students who go to bed at the bed room (11.8 %) in association between LBP and room type(P = 0.012). The statisticaly significant variables related to LBP were school division, age and stress. It was revealed by this survey that independent variables related to LBP experience rate of high school students were important health problem. So the prospective research is need by those variables.

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An Electrical Conductivity Reconstruction for Evaluating Bone Mineral Density : Simulation (골 밀도 평가를 위한 뼈의 전기 전도도 재구성: 시뮬레이션)

  • 최민주;김민찬;강관석;최흥호
    • Journal of Biomedical Engineering Research
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    • v.25 no.4
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    • pp.261-268
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    • 2004
  • Osteoporosis is a clinical condition in which the amount of bone tissue is reduced and the likelihood of fracture is increased. It is known that the electrical property of the bone is related to its density, and, in particular, the electrical resistance of the bone decreases as the bone loss increases. This implies that the electrical property of bone may be an useful parameter to diagnose osteoporosis, provided that it can be readily measured. The study attempted to evaluate the electrical conductivity of bone using a technique of electrical impedance tomography (EIT). It nay not be easy in general to get an EIT for the bone due to the big difference (an order of 2) of electrical properties between the bone and the surrounding soft tissue. In the present study, we took an adaptive mesh regeneration technique originally developed for the detection of two phase boundaries and modified it to be able to reconstruct the electrical conductivity inside the boundary provided that the geometry of the boundary was given. Numerical simulation was carried out for a tibia phantom, circular cylindrical phantom (radius of 40 mm) inside of which there is an ellipsoidal homeogenous tibia bone (short and long radius are 17 mm and 15 mm, respectively) surrounded by the soft tissue. The bone was located in the 15 mm above from the center of the circular cross section of the phantom. The electrical conductivity of the soft tissue was set to be 4 mS/cm and varies from 0.01 to 1 ms/cm for the bone. The simulation considered measurement errors in order to look into its effects. The simulated results showed that, if the measurement error was maintained less than 5 %, the reconstructed electrical conductivity of the bone was within 10 % errors. The accuracy increased with the electrical conductivity of the bone, as expected. This indicates that the present technique provides more accurate information for osteoporotic bones. It should be noted that tile simulation is based on a simple two phase image for the bone and the surrounding soft tissue when its anatomical information is provided. Nevertheless, the study indicates the possibility that the EIT technique may be used as a new means to detect the bone loss leading to osteoporotic fractures.

Development of Geometrical Quality Control Real-time Analysis Program using an Electronic Portal Imaging (전자포탈영상을 이용한 기하학적 정도관리 실시간 분석 프로그램의 개발)

  • Lee, Sang-Rok;Jung, Kyung-Yong;Jang, Min-Sun;Lee, Byung-Gu;Kwon, Young-Ho
    • The Journal of Korean Society for Radiation Therapy
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    • v.24 no.2
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    • pp.77-84
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    • 2012
  • Purpose: To develop a geometrical quality control real-time analysis program using an electronic portal imaging to replace film evaluation method. Materials and Methods: A geometrical quality control item was established with the Eclipse treatment planning system (Version 8.1, Varian, USA) after the Electronic Portal Imaging Device (EPID) took care of the problems occurring from the fixed substructure of the linear accelerator (CL-iX, Varian, USA). Electronic portal image (single exposure before plan) was created at the treatment room's 4DTC (Version 10.2, Varian, USA) and a beam was irradiated in accordance with each item. The gaining the entire electronic portal imaging at the Off-line review and was evaluated by a self-developed geometrical quality control real-time analysis program. As for evaluation methods, the intra-fraction error was analyzed by executing 5 times in a row under identical conditions and procedures on the same day, and in order to confirm the infer-fraction error, it was executed for 10 days under identical conditions of all procedures and was compared with the film evaluation method using an Iso-align$^{TM}$ quality control device. Measurement and analysis time was measured by sorting the time into from the device setup to data achievement and the time amount after the time until the completion of analysis and the convenience of the users and execution processes were compared. Results: The intra-fraction error values for each average 0.1, 0.2, 0.3, 0.2 mm at light-radiation field coincidence, collimator rotation axis, couch rotation axis and gantry rotation axis. By checking the infer-fraction error through 10 days of continuous quality control, the error values obtained were average 1.7, 1.4, 0.7, 1.1 mm for each item. Also, the measurement times were average 36 minutes, 15 minutes for the film evaluation method and electronic portal imaging system, and the analysis times were average 30 minutes, 22 minutes. Conclusion: When conducting a geometrical quality control using an electronic portal imaging, it was found that it is efficient as a quality control tool. It not only reduces costs through not using films, but also reduces the measurement and analysis time which enhances user convenience and can improve the execution process by leaving out film developing procedures etc. Also, images done with evaluation from the self-developed geometrical quality control real-time analysis program, data processing is capable which supports the storage of information.

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Antimicrobial Drug Resistance and Transferable Resistance Plasmid in Escherichia coli (대장균(大腸菌)의 항균제내성(抗菌劑耐性) 및 전달성(傳達性) Plasmid)

  • Cho, Dong-Taek;Chun, Do-Ki
    • The Journal of the Korean Society for Microbiology
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    • v.17 no.1
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    • pp.21-34
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    • 1982
  • One hundred and twenty-one strains each of Escherichia coli isolated from stools of 60 patients who received various antimicrobial drugs in hospital for more than one week and apparently healthy 60 students who have no history of taking antimicrobial drugs during recent one month, were tested for their resistance to 13 antimicrobial drugs. The frequency of resistance strains was highest to tetracycline with 69.2%, and followed by streptomycin(Sm), sulfisomidine(Su), chloramphenicol(Cm), ampicillin(Ap), and carbenicillin(Cb) in the decreasing order, ranging from 61.2% to 39.3%. Strains resistant to kanamycin(Km), cephaloridine(Cr), and trimethoprim(Tp) occupied about one-fourth of strains, and only four strains were resistant either one or more of nalidixic acid, gentamicin and amikacin, and no strain was resistant to rifampicin. The frequency of resistant strains to Cm, Ap, Km, Cr, and Cb was much higher among patient isolates than student strains, but strains resistant to the other drugs showed almost the same frequencies between patient and student isolates. There was a marked difference in average minimum inhibitory concentrations of between resistant and susceptible strains, suggesting that the resistance to drugs is the plasmid origin. Seventy-six percent of strains were resistant to one to 10 drugs tested, and no much difference was observed between strains from patients and students. However, strains resistant to four or more drugs were much more frequently found among patient isolates than student strains, with the increasing tendency of multiply resistant strains among patient isolates following the increase in the number of resistant drugs. The transfer of drug resistance by conjugation was tested and 98 strains(67.5%) among 145 which were resistant to two or more drugs were found to transfer their drug resistance to E. coli. Among 74 strains resistant to 7 or more drugs, all except one transferred the resistance, and the number of strains with transferable resistance decreased, as the number of resistant drugs decrease. A R plasmid from randomly selected p13 strain was tested for the incompatibility group, and the plasmid was classified into Inc F II. R plasmM DNA bands were identified by polyacrylamide gel electrophoresis.

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