• Journal of the Korean Chemical Society
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• v.54 no.2
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• pp.215-221
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• 2010

Salmonella is an important food-and water-borne pathogen associated with acute gastrointestinal illnesses around the world. The most common serotypes isolated from humans are Salmonella enterica serotype Typhimurium (S. Typhimurium) and S. Enteritidis. Traditional detection methods for Salmonella are based on cultures using selective media and characterization of suspicious colonies by biochemical and serological tests. These methods are generally time-consuming and not so highly sensitive. Recently, the Loop Mediated Isothermal Amplification and real-time PCR has been used as a highly sensitive, specific, and rapid test for the presence of pathogenic bacteria. In this study, a LAMP and real-time PCR was used to detect S. Typhimurium and S. Enteritidis. We selected target genes, which were the in invA and a randomly cloned sequence specific for the genus Salmonella. With LAMP and real-time PCR, random sequence was detected from Salmonella spp, invA were detected from all strain of S. Typhimurium and S. Enteritidis. This assay indicate that the specificity, sensitivity and rapid of the LAMP and real-time PCR make them potentially valuable tools for detection of S. Typhimurium and S. Enteritidis.

• Journal of the Korean Chemical Society
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• v.57 no.1
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• pp.81-87
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• 2013

Staphylococcus aureus is the most important pathogen in nosocomial infections, including bloodstream infections. Prompt identification of S. aureus from blood cultures and detection of methicillin resistance are essential in cases of suspected sepsis. We have studied a new method for the sequence-specific visual detection of minute amounts of nucleic acids using intercalating reaction by addition of SYBR Green to amplicons of LAMP, and it's a unique gene amplification method in which DNA can be isothermally amplified using only one enzyme. Staphylococcus-LAMP, which targets the spa gene, encoding S.aureus-specific protein A, and the mecA gene, encoding penicillin-binding protein-2' for methicillin resistance, detected MRSA and MRSE. In this study, by using LAMP assay, I detected for Staphylococcus aureus and Staphylococcus epidermidis concentration in the clinical sample. The detection of Staphylococcus aureus and Staphylococcus epidermidis was tested by using serial 10-fold dilutions standard solution. I have accurate detected the limit of detection, sensitity, specificity and reproducibility of the assay. The Bio-chip based LAMP assay allowed easy, rapid, accurate and sensitive detection of infection with Staphylococcus and especially applicable in a resource-limited situation.

• Korean Chemical Engineering Research
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• v.59 no.4
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• pp.565-573
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• 2021

Isotherms, kinetics and thermodynamic properties for adsorption of Brilliant Green(BG), Quinoline Yellow(QY) dyes by activated carbon were carried out using variables such as dose of adsorbent, pH, initial concentration, contact time, temperature and competitive. BG showed the highest adsorption rate of 92.4% at pH 11, and QY was adsorbed at 90.9% at pH 3. BG was in good agreement with the Freundlich isothermal model, and QY was well matched with Langmuir model. The separation coefficients of isotherm model indicated that these dyes could be effectively treated by activated carbon. Estimated adsorption energy by Temkin isotherm model indicated that the adsorption of BG and QY by activated carbon is a physical adsorption. The kinetic experimental results showed that the pseudo second order model had a better fit than the pseudo first order model with a smaller in the equilibrium adsorption amount. It was confirmed that surface diffusion was a rate controlling step by the intraparticle diffusion model. The activation energy and enthalpy change of the adsorption process indicated that the adsorption process was a relatively easy endothermic reaction. The entropy change indicated that the disorder of the adsorption system increased as the adsorption of BG and QY dyes to activated carbon proceeded. Gibbs free energy was found that the adsorption reaction became more spontaneous with increasing temperature. As a result of competitive adsorption of the mixed solution, it was found that QY was disturbed by BG and the adsorption reduced.

• Proceedings of the KIEE Conference
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• 2001.11a
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• pp.290-291
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• 2001

흡착입자간의 상호작용이 존재하는 경우 특히 최근접상호작용(nearest neighbour interaction)만이 고려된 경우의 흡착등온식(adsorption isotherm)을 구한다. 분배함수의 계산에는 Kramers와 Wannier에 의한 행열식 방법이 유용하게 사용된다.

• Proceedings of the KIEE Conference
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• 2001.11a
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• pp.288-289
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• 2001

흡착열공간분포를 외형적으로 포함하는 Heterogeneous 표면상의 흡착등온식을 형식화한다 그리고 흡착열공간분포는 위치에 대한 주기함수로서 주어지는 것임을 논한다. 본 논문에서 얻어진 결과를 재료의 박막형성 문제에 결부시켜 논의한다.

• Proceedings of the KSME Conference
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• 2005.11a
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• pp.10.2-10.2
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• 2005

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2008.11a
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• pp.488-490
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• 2008

등온 및 비등온 TGA 열분석을 이용하여 TTSP를 이용하여 실험적으로 Shift factor(Acceleration factor) ${\alpha}_T$를 구하고 이로부터 마스터 커브 (master curve)를 도출할 수 있었고, 실험에 사용된 개질된 NR고무재료는 Arrhenius식을 잘 따르는 것이 확인되었으며, 이로부터 TTSP에 의한 촉진시험 결과의 신빙성을 확인할 수 있었다. 한편, 등온 TGA 실험으로부터 얻은 활성화에너지는 37.70 kJ/mol인 반면, 비등온 TGA로부터 Kissinger plot에 의해 얻은 활성화에너지는 166.20 kJ/mol이었는데, 이는 저온과 고온에서 서로 다른 분해기구에 의해 중량 감소가 일어나기 때문인 것으로 생각 되었다.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.8 no.2
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• pp.365-369
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• 1998

Theory of the adsorption by a monolayer is extended to the multiple adsorption layers. Though the model used is extremely simple, the adsorption isotherm thus obtained describes well qualitatively the nature of unsaturation adsorptions such as $Br_2$adsorption by the silica gel.

• Transactions of the Korean Society of Mechanical Engineers
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• v.13 no.6
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• pp.1330-1338
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• 1989

본 연구에서는 직각밀폐용기의 수평경계면이 단열인 경우뿐만 아니라 등온 조건을 갖는 경우에 대해 실험적으로 연구하여 경계조건의 변화가 직각밀폐용기내 흐름 및 열전달에 미치는 영향, 특히 등온조건을 갖는 경우 수직 온도차에 따르는 안정온도 구배효과로 예상되는 흐름의 억제, 지연효과를 작종 물리적 변수들의 영향과 함께 세밀히 조사하였다.

• Transactions of the Korean Society of Mechanical Engineers
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• v.15 no.5
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• pp.1737-1746
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• 1991

본 연구에서는 저온의 순수물 속의 등온 수직원기둥에 의해 야기되는 열전달 특성을 구명함에 있어서 짧은 원기둥 범주에 속하는 종횡비 0.5인 얼음 원기둥을 이용 하여 실험적으로 열전달 특성을 구명하였다. 그리고 전 유동장을 가시화 하였으며, 얼음의 융해율로써 누셀트(Nusselt)수를 측정하여 기존의 결과들과 비교검토하였다.