• Journal of Plant Biotechnology
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• v.34 no.4
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• pp.363-373
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• 2007

To establish an efficient and reliable microspore culture system for pepper (Capsicum annuum L.), the effect of light intensity used for donor plant's growth, microspore isolation methods, the composition of culture medium, and culture period in light on the production of embryos were investigated. The viability of microspores taken from the plants grown under the light intensity of 10,000 lux was almost same as that from the lower (5,500 lux) light intensity, and the embryo induction and development were a bit higher when donor plants were grown under the lower light intensity. This result implies that lower light intensity does not interfere with the embryo induction and development. However, it was very difficult to prepare microspores for culture since only a small number of flower buds could be harvested from plants grown under the light intensity of 5,500 lux. Microspore isolation methods greatly affected microspores viability; that is, when microspores were isolated by blending rather than maceration, the greater number of viable microspores were easily generated (about 13 times). Among media used for microspores culture in this study, MN medium was most efficient for embryo induction and development. Total number of embryos and the number of cotyledonary embryos were highest when microspores were cultured in dark for 4 weeks, and then in light for one week. These results will be provide valuable information to set up efficient microspore culture system of hot pepper with a high frequency of embryo production, which are applicable to gene transformation and mutagenesis.

• The Journal of the Convergence on Culture Technology
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• v.5 no.1
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• pp.413-416
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• 2019

Alstroemeria (Alstroemeriaceae) is one of the most important cut flowers in international market. Especially, characteristics like long vase-life, various colors, tolerance to low temperature and a low energy requirement during cultivation have stimulated this success. Because of its characteristics such as low multiplication rates, time-consuming process and high risk of carrying viral disease, in vitro propagation techniques based on rhizome meristems culture have been developing nowadays. The callus induction has various cultivation sites compared with the direct plant generation method, and if the callus is maintained well, the plant differentiation can be performed simultaneously while maintaining the callus, so that it can be used for mass proliferation. In this study, we tested various hormones and cultivars for efficient callus induction. As a result of culturing between the nodes and the internodes, the callus began to be formed after 8 weeks, and the calli incidence in the nodes was higher than that between the internodes. Also, in the comparison of 2,4-D and picloram, the callus incidence rate was up to 2 times higher in the medium treated with 2,4-D. Using these results, it is thought that it will help establish the system of mass propagation system of Alstroemeria and cultivate new varieties.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1978.04a
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• pp.98.4-98
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• 1978

방선균에서 RNA 분해효소력이 강한 균주를 토양으로부터 선별하여 이 균주의 RNA 분해산물을 검토하고 부산물로서 항생물질의 생성을 조사하였다. 먼저 동시 생성 생육조건을 검토한 결과, 유기 질소원으로서 soybean meal 1.5%, 무기 질소원으로서는 $KNO_3$ 0.05% 첨가하였을 때 효과가 있었고, 탄소원으로서는 가용성 전분 2% 첨가시가 조건이 좋았다. 무기염류를 배지에 첨가하였을 때 별 다른 영향이 없었고 오히려 $CuSO_4를$ 가하였을 때는 저해를 보였다. 최초 pH가 7.0, 배양 2일째에 균체증식, 효소생산 및 항생물질의 생산이 최대를 나타내었다.

• Korean Journal of Food Science and Technology
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• v.32 no.4
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• pp.875-880
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• 2000

The production of glucoamylase and exopolygalacturonase from Cryptococcus laurentii Y-23 were investigated with the inducible substrates and mineral salts. Soluble starch induced only glucoamylase wherease pectin induced exopolygalacturonase as well as glucoamylase, and glucose did not induce glucoamylase whereas pectic acid induced a little amount of exopolygalacturonase. At the productions of two enzymes by inducible substrates for the 5 day-cultivation, the yeasts started log phase around 12 hours and mostly reached stationary phase around 36 hours. The best productivity of glucoamylase was observed with addition of soluble starch in the cultivation for 72 to 86 hours, and the high productivity of exopolygalacturonase was done by addition of both pectin and soluble starch in the cultivation for more than 72 hours. Without ammonium sulfate in the medium, however, cultural pH was so increased gradually that production of both enzymes were decreased and delayed as well. $Mn^{2+}$ increased both productivities of glucoamylase and exopolygalacturonase with 21% and 18%, respectively.

• Korean Journal of Microbiology
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• v.37 no.2
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• pp.151-157
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• 2001

This study was conducted to select lactic acid bacteria which possess potential inhibitory effect on Helicobacter pylori, and to make feasibility test of fermented milk products using them. In order to select lactic acid bacteria specifically inhibiting the growth of H. pylori, antibacterial activity using paper disk method, adherence ability to Caco-2 cell inhibitory effect on urease activity of H. pylori, and milk fermentation feasibility were measured. Among 45 strains of lactic acid bacteria tested, 28 strains showed clear zone and Lactobacillus gasseri MK-03 showed the largest clear zone. Caco-2 cell adherence by lactic acid bacteria and inhibitory effect of them on H. pylori adherence were also evaluated. Of 28 strains tested, 18 strains appeared to be effective on adherence to Caco-2 cell, and especially Bifidobacterium longum MK-26 was found to be superior to others. When Bif. longum MK-26 and H. pylori were reacted with Caco-2 cell 2hrs before, adherence percentage of H. pylori decreased from 0.105% to 0.004%. To investigate inhibitory effect of lactic acid bacteria-derived supernatant on urease activity of H. pylori, pH-adjusted fermented supernatant(pH-4.4) was assessed by co-cultivation method. There of Lb. acidophilus MK-07-derived supernatant showed the most inhibitory effect on urease activity of H. pylori. Considering milk fermentation ability of selected 3 strains, they were comparably feasible to fermented milk products. Consequently, Lb. gasseri MK-03, Lb. acidophilus MK-07, and Bif. longum MK-26 were selected to specifically inhibit the growth of H. pylori, by antibacterial activity, inhibition of urease activity, and inhibition of Caco-2 cell adherence, respectively.

• Journal of Animal Science and Technology
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• v.55 no.1
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• pp.41-49
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• 2013

In vitro and in situ incubation studies were conducted to determine effects of yeast culture supplements (Saccharomyces cerevisiae) on cellulolytic bacterial function and fiber digestion in rice straw. In vitro dry matter digestibility of rice straw gradually increased according to supplemental levels of yeast culture (0.0, 0.2, 0.4, 0.6, 0.8 and 1.0%). Digestibility of rice straw started to increase apparently when yeast culture was added more than 0.6% level (p<0.05). Also, we reconfirmed that in vitro dry matter digestibility was significantly increased by 0.6% of yeast culture addition in 4% NaOH treated and non-treated rice straws (p<0.05). When in situ dry matter digestibility was tested in Korean native goats fed basal diet or experimental diet which contained 1.0% of yeast culture, the yeast culture feeding improved in situ dry matter digestibility in both 4% NaOH treated and non-treated rice straws (p<0.05). In case of real-time PCR monitoring cellulolytic bacterial function, the bacterial population attached on rice straw showed the increasing trends with higher level of yeast culture spraying on rice straw. F. succinogenes and R. flavefaciens were significantly increased in accordance to spraying levels of yeast culture (0.0, 0.1 and 0.3%) at both 12 and 24 hrs of in situ incubation (p<0.05). R. albus was significantly higher population in yeast culture spraying than non-soraying at 12 hrs of in situ incubation (p<0.05). These bacterial populations were showed the increasing trends with digestibility enhancement of rice straw according to the higher levels of yeast culture supplement. Overall, these results clearly suggest that the presence of yeast culture result in noticeable increase of rice straw digestion, which is modulated via good effect on cellulolytic bacterial attachment to fiber substrates.

• The Microorganisms and Industry
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• v.20 no.3
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• pp.49-59
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• 1994

효소관련 각종 특허를 국가별, 산업적 응용면, 또는 효소기술상으로 분류하여 통계적으로 분석을 해 봄으로써 여러가지 현상을 발견 내지 추론해 볼 수 있다. 첫째, 연도별, 국가별 출원현황 및 경향을 알 수 있으며 둘째, 이러한 특허출원들이 산업적으로 어떤 분야에 적용 내지 적용가능한 지를 파악할 수 있고 셋째, 기술적으로도 어누 부문에 치중 내지 편중되어 출원이 이루어지고 있는 지를 알 수 있다. 그리고 보다 중요한 것은 이러한 여러 측면을 동시에 고찰해 봄으로써 효소특허출원 경향에 관한 통찰력의 배양을 통해 연구자가 연구개발의 흐름을 파악해 볼 수 있다는 것이다.

• Proceedings of the Korea Society for Industrial Systems Conference
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• 1998.10a
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• pp.149-158
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• 1998

교육 환경의 변화와 정보 기술의 변화에 따라 정보 교육을 효율적으로 실시하기 위하여 정보 교육 시설의 설치에 대한 필요성과 이를 활용한 정보 교육의 효율성을 극대화하기 위한 연구가 필요한 시점이다. 본 고에서는 정보 교육의 목표와 오늘의 현상 및 문제점을 분석하고 동시에 "정보소양능력"을 배양하기 위한 '정보 교육"을 위한 정보교육 시설의 구비 요건, 구축 모델 제시, 바람직한 운영방법을 제시하고자한다. 운영방법을 제시하고자한다.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1974.06a
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• pp.123.3-124
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• 1974

주정 제진공장에서 배출되는 형액을 이용해서 수입어분 및 수입효모를 대체할 수 있는 SCP를 생산하며 동시에 발액을 처리하고자 하였다. 밀 주정폐액을 여과한 결과, 여액의 조성은 당 1.54％, 총질소 0.39％, 회분 0.43％, pH가 3.15이었다.(중략)

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.396-397
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• 2000

$TGF-{\beta}_1$을 insect cell-baculovirus system을 이용하여 생산하였으며, 이 경우 낮은 세포밀도와 암모니아 같은 노폐물에 의한 생산성 저해 문제를 해결하기 위해 유가식 배양과 흡착제에 의한 암모니아 제거를 동시에 수행함으로써 $TGF-{\beta}_1$의 생산성을 향상시킬 수 있었다.