• Korean Journal of Applied Biomechanics
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• v.13 no.3
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• pp.47-65
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• 2003

The purpose of this study was to investigate the differences of the kinematical and the kinetical factors that calculated from preflight to postflight of salto forward straight 3/2 turn motion between skitters and less-skitters. four S-VHS video cameras operating at 60Hz were used to record the performances. five elite male gymnasts were participated in this study as subjects. three-dimensional coordinates of 20 body landmarks during each trial were collected using a Direct Linear Transformation method. The digitized body landmarks were smoothed using a Butterworth second order with low pass digital filter and a cutoff frequency of 10Hz. 1. A skitter, got a high score for performance, showed shorter time and faster horizontal velocity than a less-skitter at the board contact. also, a skitter extended quickly his knee and hip joint after contacting board for preflight phase. 2. A skitter revealed faster time and horizontal velocity the vault from taking off board than a less-skiller. A skitter took a long time and high distance to get the vertical peak compared with a less-skiller. 3. For the second phase, a skitter, who executes the most optimal motions among the subjects, displayed a long flight time, a high height, and a far flight distance as well as maintaining consistent horizontal speed even at the peak of post flight. On the other side, a less-scorer displayed a slow vertical velocity, distance and a short time at the point of take-off from vault as well as low height at the peak of post flight.

• Biomedical Science Letters
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• v.1 no.1
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• pp.1-8
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• 1995

The present study was undertaken to determine the dissociation constant (Kd)of monoclonal antibody to human apolipoprotein A-I (apo A-I) using enzyme-linked immunosorbent assay (ELISA). First the monoclonal antibody was incubated in solution with the antigen until the equilibrium was reached; then the free antibody which remains unsaturated at equilibrium was captured by binding to antigen on the microtiter plate and be measured by a classical indirect ELISA. The value of Kd determined from Scatchard plot was 0.625$\times$10^{-9}$ for purified antibody and 0.720$\times$10$^{-9}$ for unpurified antibody. This method was valuable for the measurement of true dissociation constant and found to be simple, reproducible, and accurate.

• Korean Journal of Microbiology
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• v.4 no.2
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• pp.5-10
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• 1966

The relationships between tumor score and peroxidase activities of tomato stems infected with Agrobacterium tumefaciens strain A6Kl, B6, T372 11BNV6, 11BV7 and wounded stem as a control were examined in relation to crown gall tumor development on purpose to study the lignification of tumor tissue which is affected to the development of crown gall tumor. As the previous paper has been mentioned the fact that the induction of tumor tissues were inhibited or limited in the lignified stem of host plant. It was presumed that the activities of peroxidase related to the development of lignification were decreased during the period of tumor development. But the experimental result in this experiment shows that the peroxidase activities of crown gall tumor-tissues infected with the A. tumefaciens strains which are already known as virulent are increasing during four weeks, however, in the strain 11BNV6 and wound the peroxidase activities are decreasing on the second week after the inoculation of the bacteria strains. These results could be explained on the basis of that possible regulatory agents of lignification which were accumulated in tumor tissues, IAA, ascorbic acid, glutathion(GSH) and caffeic acid esters, were postulated to act as antioxidants which has been suggested by Stafford. Total nitrogen contents in relation to crown gall tumor development were determined for the detection of protein synthesis related to the enzyme activities which are increasing in the time of plant growth. Generally six groups are contained the largest amount of nitrogen on the second week after the inoculation of the bacterium. Comparing to the tumor score, it is presumed that the all of enzyme activities including peroxidase in tumor tissues are increasing from the second week through the third week after the inoculation of bacterium and the protein synthesis is stimulated under the most appropriated temperature during the above periods.

• Microbiology and Biotechnology Letters
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• v.17 no.1
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• pp.19-23
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• 1989

A sequential system composed of hydroxylapatite chromatography and gel permeation chromatography was developed to purify the IgM type monoclonal antibody against the colon cancer cell SC-1 from the ascitic fluid of mice injected with the murine hybridoma CH07E02. In the hydroxylapatite chromatographic step the band dilution could be reduced by controlling the gradient and flow rate of the eluent, the sodium phospate buffer, the optimum values for these variables being 5.82$\times$10$^{-3}$M/cm and 0.2$m\ell/\textrm{cm}^2$/min, respectively. A degree of purity better than 99.99% as judged from silverstaining of the SDS-PAGE bands, was obtained by adding the gel permeation chromatographic step in tandem.

• Microbiology and Biotechnology Letters
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• v.14 no.6
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• pp.433-439
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• 1986

Abundant amount of Chiamydia trachomatis could be amplified in mammalian McCoy cells and purified using descontinuous Uroarafin gradient centrifugation. As a chemical means io increase the Chlamydia trachomatis inclusions in McCoy cells IUdR treatment was found to be more effective than the cycloheximide treatment and was recommendanble for the proliferation of Chlamydia trachomatis. Centrifugation promoted Chlamydia trachomatis adhesion to McCoy cell surface, and maximal percentage of infected cells was obtained at about 3000g. The purified Chlamydia trachomatis could be kept in SPG solution for 48 hours at +4$^{\circ}C$ but for longer storage freezing to -7$0^{\circ}C$ was necessary.

• Food Science and Preservation
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• v.19 no.1
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• pp.54-61
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• 2012

According to lifestyle changes, the consumers' concern about food also shifts from calories and nutrition to health and convenience. Fresh-cut produce is one of the new turns in the consumption pattern of fruits and vegetables. The increasing demand for it requires processors to make them stable in quality and safe from microorganisms. The results of the evaluation of the production process and hygienic management of fresh-cut lettuce revealed that the facilities used, such as the drainage holes, floors, and door knobs, were severely contaminated with microbes, and that the work equipment, workbenches, landing nets, and centrifuges were highly contaminated. Accordingly, improved production processes and management systems are necessary, as is the implementation of a quality control system from the stage of raw-material purchase to the distribution stage.

• Journal of Haehwa Medicine
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• v.14 no.2
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• pp.177-187
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• 2005

Objective : This study is designed in order to evaluate the therapeutic effect of Scalp Acupuncture and Do-ma Acupuncture related with Governer Vessel(Dok) and Bladder Meridian(Chok-taeyang) on acute low back pain Methods : We investigated 10 patients suffering from acute low back pain were treated from Mach. 1st 2005 to Jun. 30th 2005. 10 patients, were diagnosed only straightened curvature on lateral view of lumbar spine X-ray and get Scalp acupunture related with Governer Vessel(Dok) and Bladder Meridian(Chok-taeyang) and Do-ma acupuncture. Pain rating score(PRS) was used to assess the pain for its intensity, frequency, duration, and aggravating factors. And visual analog scale(VAS) was used, too. Results : Both Scalp acupunture related with Governer Vessel(Dok) and Bladder Meridian(Chok-taeyang) and Do-ma acupuncture therapy showed good effect on acute low back pain. It was proved by the difference between PRS and VAS checked before treatment and what checked after treatment. In the evaluation of treatment effect, Exellent was 2(20%), Good was 5(50%), Moderate was 3(30%), Poor was 0(0%). Conclusion : The therapeutic effect of Scalp Acupuncture and Do-ma Acupuncture on acute low back pain can be recommended as a useful therapy to treat acute low back pain.

• Korean Journal of Applied Biomechanics
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• v.14 no.1
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• pp.13-26
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• 2004

The purpose of this study was to investigate the differences of the kinematical and the kinetical factors that calculated from preflight to preflight of salto forward straight 3/2 turn motion between skillers and less-skillers. four S-VHS video cameras operating at 60Hz were used to record the performances. Five elite male gymnasts were participated in this study as subjects. Three-dimensional coordinates of 21 body landmarks during each trial were collected using a Direct Linear Transformation method. The raw 3-D coordinates of the 21 body landmarks were smoothed using a second order lowpass, recursive Butterworth digital filter and a cutoff frequency of 10Hz. Load cells attached on the beneath of a board were used to attain the kinetic variables. It was found that the more angular momentum in the longitudinal axis, the less vertical velocity and these angular momentum effected the height of peak in the preflight. Also, it was revealed that the larger angular momentum in the medio-lateral axis was rather than it in the longitudinal axis to increase vertical height and rotation force of the body. For the reaction force of springboard, the vertical and the horizontal reaction force were 16.52BW and 3.45BW, respectively. It was found that the higher value of the vertical reaction force induced the faster vertical velocity and the higher an ar momentum. of the whole body center of gravity.

• Korean Journal of Applied Biomechanics
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• v.15 no.4
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• pp.143-151
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• 2005

This study was conducted to investigate the technical factors of Cuervo forward straight vaults with single twist, single and half twists, and double twists actually performed by three execellent male gymnasts participated in artistic gymnastics competition of 2003 summer Universiade in Daegu and the 85th National Sports Festival in Cheongju. To accomplish the research goals the Cuervo vaults of three gymnasts were filmed by using three digital camcorders set by 60 Hz, and data were collected through the DLT method of three dimensional cinematography. The kinematic and kinetic variables as each phasic time, CM displacement velocity, release angle inclination angle hip joint angle landing angle, average horse reaction force average moment arm average torque, whoe body's total remote local angular momentum were analyzed, so the following conclusions were reached. Generally to perform the better Cuervo vault, a gymnast should touch down on the board with the great horizontal velocity of the whole body through the fast run-up, and touch down on the horse by decreasing the horizontal displacement of the whole body during the preflight, so raise CM height gradually within a short horse contact time. He should increase the horse reaction force through checking the horizontal velocity of the whole body effectively and the inclination angular displacement of the handstand, if so he can have the large vertical velocity of the whole body. By using the acquired the velocity and the angular momentum of the whole body, he can vault himself higher and twist sufficiently, then he can get better if the body could be tilted by swinging both arms and perform the cat twist with a little flexions at hip joints. According to the above outcomes we can judge that the best athletes is LuBin, the better is YTY, and the next is JSM.

• Journal of Wetlands Research
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• v.5 no.2
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• pp.57-65
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• 2003

This study was corried out to provide basic data for water analysis biodiversity, Actual vegetation map, flora and fauna on the Sanchon-tideland(Geojaesi), Doma-tideland(Namhaegun) in Kyungsangnamdo from august 2002 to February 2003. This tidelands was very broad and was being reclaimed. Therefore, it was anxious about destroying the habitat of the water birds. Since the water temperature was influenced by the surrounding air, it was highest in summer and lowest in winter. pH value and DO concentration of the water was highest in winter in the Sanchon-tideland and in surmmer in the Doma-tideland. COD concentration was lowest in winter in two tidelands. SS concentration was highest in summer. T-P and T-N concentration were very low but decreased with the seasonal change from summer to winter. Vascular plants of this area consist of 11 varieties, 67 species, 64 genera, 29 families(Sanchon-tideland) and 1 forma, 11varieties, 62 species, 64 genera, 30 families(Doma-tideland). The predominant community of this area, Phragmites communis Community were found on the seashore. The total insect specimens were classified into 120 species, 48 families, 10 orders(Sanchon-tideland) and perdominant species were Gabala argentata(Dominance 18.78%, Diversity index 1.75, Richness index 45.78). And 91 species, 40 families, 8 orders(Doma-tideland) and perdominant species were Sternolophus (Sternolophus) rufipes(Dominance 15.47%, Diversity index 1.47, Richness index 33.66). The total Fishes were classified into 3 species, 2 families, 1 order and Amphibia were 3 species, 2 families, 1 order. And Salicomia herbacea should be worthwhile species to conserve in this area. Because the water birds lived in The Tideland Sanchon and Doma, it was considered that it was necessary to preserve them.