• Journal of fish pathology
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• v.26 no.3
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• pp.241-254
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• 2013

In order to establish bio-marker systems for the screening of endocrine-disrupting chemicals contaminated in various environment, Vitellogenin(Vtg) bio-marker have been developed to detect Scorpion fish's(Sebastiscus marmoratus) Vtg. Vtg has been induced by administration of estradiol into S. marmoratus, and purified by gel filtration and ion-exchange chromatography from serum of the fish. After immunization of the purified Vtg into BALB/c mouse, hybridomas secreting anti-Vtg antibodies have been produced. The size of induced Vtg in the serum was about 440 kDa by gel filtration using Sepharose CL-6B. By SDS-PAGE analysis, the main band of Vtg, however, was at 175 kDa, and several minor bands have been detected with the main band. Eight different monoclonal antibodies have been produced from established hybridomas and the antibodies did not cross-react with sera from different species of fishes tested in this study except with that of Sebastes hubbsi. These results suggested that the monoclonal antibody of S28 and S15 can used as capture and tracer antibodies for ELISA and ICG assays. The detection systems developed in this study can be used as Bio-marker assays to check endocrine disrupting activity of various chemicals as well as to detect known endocrine disrupting chemicals contaminated in environment.

• Journal of the Korea Fashion and Costume Design Association
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• v.13 no.2
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• pp.27-34
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• 2011

Creative application of exaggerated measurements and origami principles can make clothing design unique and formative clothing design possible. There have been many techniques applied to formative clothing design, but, in particular, origami principles, in company with exaggerated measurements, play a significant role in enlightening clothing uniqueness and formativeness in contemporary fashion design. The primary purpose of this study was to make a new suggestion for the production of high value-added fashion by reviewing and synthesizing exaggerated measurements and origami principles. Diverse relevant designs were retrieved through a comprehensive literature review on topic-related theses, books, and fashion media from 2000 and significant cases were retrieved from Rick Owens, Damir Doma & Haider Ackermann out of 2008 & 2009 F/W collections. From a comprehensive review, the following formative characteristics of exaggerated measurements and origami principles applied to clothing design were identified: (1) expandability, (2) fluidity, (3) deconstruction, and (4) irregularity. In addition, the following value of exaggerated measurements and origami principles applied to clothing design were identified: (1) alterability, (2) form potentiality, and (3) contradistinctive formativeness.

• Korean Journal of Environment and Ecology
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• v.20 no.3
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• pp.352-358
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• 2006

We reconsidered the taxonomy of lizards in Korea on the basis of morphological characters of collected specimens and previously published data. A total of 138 specimens were used in this study, which were collected from April 1996 to October 2005, From the observations, we presented keys to classify Korean lizards. Also, we indicated sampling sites for each species on the Korean map. In addition, we added some remarks on the distribution of Gekko japonicus, the study of genus Scincella and the synonym of genus Takydromus. Especially, it was uncertain whether the southern Korean Peninsula is a natural habitat of Takydromus tachydromoides oldi which will be necessary in further study.

• Proceedings of the Korean Institute of Landscape Architecture Conference
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• 2017.10a
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• pp.72-75
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• 2017

도시농업 및 커뮤니티에 대한 사회적 관심에 편승하여 근래 도시의 커뮤니티가든(Community Garden)이 주목받고 있다. 북미의 커뮤니티가든은 농작물 생산 위주의 개인텃밭을 포괄한 지역사회의 구성원과 함께 만들어가는 오픈스페이스의 형태로, 화훼 등을 포괄하는 개념으로 간주되고 있다. 본 연구에서는 커뮤니티가든의 발상지인 북미 중에서도 가장 선진적인 사례로 손꼽히고 있는 미국 워싱턴주 시애틀(Seattle)시의 피패치(P-Patch)를 대상으로, 85개의 현장 전수조사를 통하여 공간설계 및 디자인요소를 추출하여 시사점을 도출하였다. 분석결과, 첫째 시애틀 피패치의 일반적인 디자인 구성요소는 기반시설, 지원시설, 휴게시설, 특수시설, 친환경시설 등 다섯 가지 기능으로 대별할 수 있는데, 지원시설로는 공구함(Tool Shed)이나 수전시설(Spigot)이, 친환경시설로는 도마(Cutting Board)나 퇴비함(Compost Bin)이 대부분 설치되어 있다. 둘째, 피패치의 공간구성 및 디자인적 특징으로는 이용자 수요 및 입지적 특성을 반영한 유연한 정원 설계를 들 수 있다. 셋째, 피패치는 다목적형 기능을 겸비한 복합적 공공장소로서 공공의 제도적 지원과 시민운동을 연계하는 기회를 제공하고 있다. 넷째, 지속가능성을 담보하기 위해 다양한 이해관계자(이용자, 코디네이터, 운영진, 전문가, 지역주민, 자원봉사자 등)와의 파트너십 구축, 다양한 재원 조달 방안 모색, 체계적인 모니터링 시스템 구축 및 운영을 지속하고 있다.

• KSBB Journal
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• v.8 no.5
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• pp.478-482
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• 1993

Perfusion culture was conducted in Celligen perfusion culture system using a self-constructed hybridoma cell and low serum medium. The culture system employed hollow fiber to separate cells from the culture broth. Maximum cell density of $2.1\times10^7$ ce11s/m1, 10 times higher than in batch culture, could be achieved. Concentration of monoclonal antibody (MAb) was 4 times higher and production rate at maximum feed rate was 9 times higher than in batch culture. Glucose concentration was very important for the cell growth and MAb production. When glucose concentration was below 1g/l, i. e. 0.5~0.9g/l, specific MAb production rate decreased but cell concentration still increased. As the glucose concentration goes above 1g/l, specific MAb production rate increased and remained at maximum value at more than 1.5g glucose/l. The maximum value of the specific Mab production rate was similar to that of batch culture.

• Korean Journal of Applied Biomechanics
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• v.13 no.3
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• pp.67-79
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• 2003

The purpose of this study is to investigate the effective skill motion through the kinematic analysis of the Yurchenko Streched motion in new horse vaulting. The subjects in this study were 3 male gymnasts who were ranked as national athletes. After the 3D motion analysis, kinematic variables were analyzed to comparison with the difference between this study and the previous study(Yang, D. Y and Lee, C. S, 1999). As a result, the following conclusions was drawn; 1. In the board contact phase, this study showed a shorten contact time to maintain in condition highly extension of hip and hee angle than the previous study. 2. In the pre-flight phase, this study appeared more shortly flight duration time and horizontal flight displacement than the previous study. 3. In the horse contact phase, the contact duration time and horizontal displacement of COG shortened than the previous study, but appeared to the fast horizontal and vertical velocity and highly extension of shoulder an. 4. In the post-flight phase, a stable horse contact appeared to the increase of the flight duration time and the apex height during the post-flight. And it showed that these results have a stable and good landing.

• Korean Journal of Applied Biomechanics
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• v.16 no.4
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• pp.135-145
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• 2006

The aim of this study was to find out the differences of biomechanical factors from touching down the vaulting board to landing when techniques of 1/1Turn, stretched, and Tucked were performed on the old vaulting horse and on the new vaulting table. Three national representative men gymnasts were sampled for this study. Three dimension motion analyses by means of six Sony PD-150 video cameras with the velocity of 60 fps were used. As a result of analyzing the kinetic data from two kind of vaulting table, the following conclusions were made. 1. There was not significant differences of angular momentum between the old and the new vaulting table in all three techniques except the phase of stepping on the vaulting board and contacting the vaulting horse in the Tucked technique. IN the two phases above, the angular momentum in the new vaulting table was greater than that of the old vaulting horse. 2. There were few significant differences between the old and the new vaulting horses in the horizontal and vertical reaction force according to techniques when stepping was performed. However, it appeared tendency that the horizontal and vertical reaction force in the new vaulting table was a little greater than that of the old vaulting horse when the 1/1Turn, the Stretched and the Tucked were performed.

• Korean journal of applied entomology
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• v.16 no.2 s.31
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• pp.101-104
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• 1977

Potato virus X was purified especially for the preparation of antisera for diagnosis and identification. Potato virus X was isolated Iron infected plants by means of indicator plants and identified in electron microscopy. Isolated PVX was multiplied in tomato plants and purified by a modified procedures. The purity of PVX was 0.59mg/m1. Purified PVX was injected into rabbits once a week for 5 weeks. Antiserum was collected 10 days after the last injection. Produced antiserum was determined 1/1024 titers by means of microprecipitin tests and showed sharp reactions in agar gel-diffusion tests.

• Korean Journal of Agricultural Science
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• v.18 no.2
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• pp.99-103
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• 1991

The pathogenic bacteria isolated from the rotted fruits and tubers of red pepper, tomato, eggplant and potato which were collected from farms of Chungnam province and the markets of Seoul, Pusan, Taejon, Chonju, Iri, Weonju, Jecheon, Chungju and Cheonan in Korea. These isolates were divided into ninety-eight isolates of Enoinia sp. and seven isolates of Pseudomonas spp. on the basis of their taxonomic characteristics. Among seven isolates of Pseudomonas spp., six isolates were identified as P. viridiflava and two were P. marginalis and ninety-eight isolates of Erwinia sp. as E. carotovora. subsp. carotovora on the basis of bacteriological properties.

• Korean journal of applied entomology
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• v.11 no.1
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• pp.5-9
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• 1972

The experiment was conducted to investigate the physiological characteristics on 16 Isolates of bacterial wilt pathogen, pseudomonas solanacearum E.F. Smith, those obtained from infected stems of tomatoes, hot-Peppers and eggplants. PSA and Sucrose medium favoured by the most of the isolates, and various degree of gelatin liquefaction occurred by each of nine isolates those alble to liquefy gelatin among 16 isolates tested. The most of the isolates except one, did not reduce methylen blue. All isolates did not utilize lactose, saccharose, and starch, although all isolates utilize the galactose. The utilization of dextrin, esculin, glucose, mannitol, raffinose and salicin was depended on each isolate.