• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.71-71
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• 2022

들깨는 잎은 채소, 종자는 기름과 향신료로 이용하는 특용작물로 국립농업과학원 농업유전자원센터에는 국가등록 3,066자원을 보유하고 있다. 들깨 유전자원의 보존기간과 방법에 따라 종자 활력의 차이가 어떻게 변화했는지 알아보기 위해 농업유전자원센터 보존 중인 들깨 유전자원의 발아율을 조사하였다. 온도는 4도, -18도, 밀폐용기 보관하여 보존한 들깨 종자를 10년 보존 후 발아조사를 수행하였다. 발아환경은 페트리디쉬에 거름종이를 깔아 멸균수로 수분을 공급하고, 종자를 50립씩 2반복 치상 후 20도에서 7일, 14일에 조사하였다. 4도에서 보존한 들깨 577자원은 발아율이 85%이상인 자원이 184자원으로 나타났다. -18도에서 보존한 들깨 725자원은 발아율이 85%이상이 자원이 329자원으로 나타났다. 세계적으로 식물유전자원은행에서 양호한 발아율의 기준으로 85%를 사용하는데, 4도 보존 들깨 유전자원들은 조사한 자원의 32%가 양호하였고, -18도 보존 자원들은 45%가 발아 기준을 만족하였다. 들깨유전자원을 10년 주기로 발아율을 조사한 결과 -18도 보존 종자의 발아율이 4도 보존 종자보다 기준발아율이 13% 우수하였으나 두 처리 모두 85% 기준발아율을 만족하는 자원이 50%이하로 나타났다. 본 연구결과는 들깨유전자원을 4도, -18도에서 장기 보존할 때에 종자 활력 예측 및 활력 모니터링 연구에 도움이 될 것이다.

• Journal of the Korean Applied Science and Technology
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• v.36 no.1
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• pp.226-236
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• 2019

In this study, functional and emulsifying properties of oil extracted by supercritical carbon dioxide from balloonflower(Platycodon grandiflorum A. DC) seeds were investigated. The oil was lower in total polyphenol content(8 mg/100 g), but higher in ${\alpha}$-tocopherol(14.15 mg/100 g), ${\beta}$-sitosterol(116 mg/100 g) and stigmasterol(8 mg/100 g) contents than seeds. Based on DPPH radical scavenging activity, the oil($IC_{50}=1235.5{\mu}g/mL$) showed similar antioxidant activity to the seeds($IC_{50}=1138.2{\mu}g/mL$). At 1%(w/w) lecithin, O/W emulsion with balloonflower seeds oil had turbidity, microscopic image, mean particle size and emulsion stability similar to emulsion with soybean oil, but had lower turbidity and emulsion stability and larger mean particle size than emulsion with perilla seeds oil. Its surface tension was similar to perilla oil emulsion.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.67-67
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• 2022

먹넌출은 국내에서 안면도에서만 자생하는 희귀식물로, 항산화, 미백, 항암 효능이 밝혀져 유용식물자원으로의 활용이 기대되나 IUCN(세계자연보전연맹) 기준 취약종(VU)으로 분류되어 생명자원의 현지 내, 현지외 보존 필요성이 크다. 따라서 본 연구는 먹넌출의 종자를 이용한 보존과 증식을 위해 기초자료를 확보하고자 하였다. 이를 위해 열매의 성숙도에 따른 형태적 특성과 활력 차이를 비교하고, 종자의 발아 조건 구명을 위해 휴면 종류와 발아 적온을 조사하였다. 먼저 열매 성숙도에 따른 형태적 특성과 활력 조사 결과, 열매가 부분적으로 성숙한 적색 단계는 완전히 성숙한 흑색 단계와 비교하여 종자 무게와 배길이에서 차이가 거의 나타나지 않았고 충실도가 80%, 충실 종자의 활력이 100%로 나타났다. 따라서 종자 채취 시 열매가 흑색으로 성숙하여 떨어지기 전인 적색 단계에서 채취하여도 종자를 이용한 증식에 문제가 없을 것으로 보인다. 또한 먹넌출 종자는 딱딱한 과피로 덮여있지만 수분 흡수를 가능하게 하는 조직을 가지고 있어 물리적 휴면에 해당하지 않았으나 생리적 휴면을 가지고 있었으며, 휴면 타파에는 GA3 1000ppm 처리가 가장 효과적이었다. 또한 발아 온도는 10℃ 12시간, 20℃ 12시간인 변온 조건에서 가장 높은 발아율을 보였다. 본 결과를 먹넌출 종자 수집과 증식에 참고 자료로 활용할 수 있을 것으로 기대한다.

• Korean journal of applied entomology
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• v.21 no.1 s.50
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• pp.23-26
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• 1982

The present study was carried out to investigate the change of peroxidase activity of soybean seed infected with Cerrospora kikurhii. The protein content, polyphenol oxidase activity and peroxidase isozyme pattern in health and infected soybean seed were also compared. 1. The peroxidase activity was substantially higher in the infected soybean seeds than that in the healthy seeds either cracked or not. No significant differences in protein content were recognized among the seeds tested. 2. No significant differences in peroxidase activities and protein contents were notified between healthy and infected seeds from the measurements on each parts of dissected seeds, cotyledon and seedcoat, however the peroxidase activity in the seed coat of the stained seed was 2.5 times to healthy seed. 3. The activities of polyphenel oxidase were undectable in both healthy and diseased seeds. 4. The electrophoretic Patterns of the Peroridase isozyme were the same between healthy and in footed seed. 5. Therefore, the increase of peroxidase activity in infected soybean seedcoat was mainly due to the biochemical reaction against the pathogen.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2004.05a
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• pp.137-140
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• 2004

채종원관리를 효율적으로 관리하기 위하여 시설되는 저 규격도로는 대부분 비포장상태로 이용되는 경우 많다. 이는 강우에 의한 침식붕괴로 하류의 산림식생환경 및 수질환경 오염으로 연결될 위험성이 내포되어 있어 적절한 방법으로 사전에 방지할 필요가 있다. 본 연구에서는 환경과 친화적인 재료를 최대한 이용하여 개발된 목배수로를 내구성 강화를 검토하였던바 섬유질 포의 조절과 고무질의 성능 조절에 의한 검토로 현장에의 적용이 가능하였다.

• Journal of the Korean Applied Science and Technology
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• v.35 no.2
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• pp.478-484
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• 2018

In this study, proximate composition, crude fiber, reducing sugar, free sugars, organic acids, minerals and amino acids of Platycodon grandiflorum seeds were analyzed to evaluate its nutritional value. Moisture, crude protein, crude fat, crude ash and carbohydrate contents of seeds were 6.97, 26.05, 27.46, 3.78 and 35.74%, respectively. Crude fiber of 6.31% and reducing sugars of 1.54% were also determined. Sucrose(1,661 mg/100 g) and lactic acid(1,224 mg/100 g) were most abundant free sugar and organic acid, respectively. Both phosphorus and potassium were main minerals that contained more than 700 mg in 100 g seeds. Amino acids analysis of 100 g seeds showed that glutamic acid(3.45 g), arginine(2.51 g), aspartic acid(1.66 g), leucine(1.29 g), lysine(1.10 g), alanine(1.05 g) and glycine(1.04 g) were abundantly contained in order, while others were less than 1 g.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.285-290
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• 2009

This experiment was conducted to investigate the propagation system by seed to check growth, flowering, and making seed of three-year-old cactus of potted Mammillaria goldii. M. theresae, and M. pseudopeclinata. Plant height and width of them was 2.5~2.8 cm, and 2.8~3.1 cm, and seed formation of Mammillaria goldii and M. theresae had internal body taking130 to 135 days to ripening seeds, however, M. pseudopectinata had externals taking just 26 days to ripening seeds. The mean seeded pod from April to June of M. goldii was 50.0% and the number of seeds in a pod was 25.3. In M. theresae, the mean seeded pod from April to June was 57.3% and the number of seeds in a pod was 35.0. However, the highest rate of seeded pod on May of M. pseudopectinata was 75.0% and the number of seed in a pod was 66.0. The sib crossing was much more ripening seed fertilization than that of self cross ing in the Mammillaria. The self crossing was formed from 12.0% to 20.0% of seeded pod, from 12.0 to 16.0 number of seed in a pod. However, sib crossing obtained 58.0% of seeded pod, 30 seeds in a pod of M. goldii, 65.0% seeded pods and 40 seeds in a pod of M. theresae, and 75.0% seeded pod, 68 seeds in a pod of M. pseudopectinala as the highest of them.

• Agrochemical news magazine
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• v.23 no.3 s.176
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• pp.14-17
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• 2002

• Korean Journal of Medicinal Crop Science
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• v.10 no.2
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• pp.75-81
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• 2002

Indoor seed treatments to elevate seedling emergence should be of value. The study was done to model the presown treatments of Platycodon grandiflorum seeds by evaluating the treatment effects of priming,$GA_3$, drying and water imbibition after drying on their germination and then their successive seed treatments on the basis of its seedling emergence. after priming using $Ca(NO_3)_2\;and\;GA_3$ treatment under their different concentrations and light quality illuminated for 12 hours a day were done separately and their two best results were compared to determine the better one, drying of imbibed seeds using the above best result and water imbibition of the dried seeds were successively done to check the rates of germination and emergence. In each treatment of priming and $GA_3$, the former best germination occurred at $Ca(NO_3)_2$ 150 mM done under 2 day darkness but the latter one did at 0.01 mM done under 12 hour a day red light forced for 3 days. Of the two best results from priming and $GA_3$ treatments, the latter result was shown higher germination rate. $GA_3$ treated seeds were best desiccated under $35^{\circ}C$ and 4 hour red light illumination. The germination rate of seeds dried after $GA_3$ treatment was enhanced as imibibed 2 days immediately before sowing. Seedling emergence of all 3 successive treatments, $GA_3$, drying and water imbibition before sowing was the greatest than the two others, only $GA_3$ treatment and the combination of $GA_3$ and drying, in which indicated that its presown seed treatment must follow the successive procedure of the above 3 ones.

• Korean Journal of Food Science and Technology
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• v.31 no.6
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• pp.1410-1414
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• 1999

The granular size and shape of durian seed starch were $2.0-10.0\;{\mu}m$ and oval and polygonal. Amylose contents of durian seed, corn, sweet potato and potato starch were 28.3%, 27.5%, 20.3% and 21.7%, respectively. Blue value of durian seed (0.370) higher than that of corn (0.368), sweet potato (0.332), and potato starch (0.338). Alkali numbers of durian seed, corn, sweet potato and potato starch were 7.39, 9.02, 7.08 and 5.43, respectively. Swelling power of durian seed starch was similar to that of sweet potato starch. X-ray diffraction patterns of durian seed starch showed an A-type crystalline structure. According to pasting properties by Rapid Visco-Analyzer, the gelatinization temperature of durian seed starch $(76.6^{circ}C)$ was higher than that of corn $(73.0^{circ}C)$, sweet potato $(72.3^{circ}C)$ and potato starch $(70.2^{circ}C)$. The breakdown of durian seed starch were lower than that of corn, sweet potato and potato starch.