• Korean Journal of Ecology and Environment
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• v.41 no.2
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• pp.117-127
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• 2008

Green algae are important primary producers in aquatic ecosystem, and they are sensitive test species in bioassay. Green algae are broadly used to assess the adverse effects of various chemicals by measuring the inhibition of metabolism, reproduction and survival. In this study, we extensively gathered domestic and foreign toxicity test methods conducted using green algae, which are distributed in Korean water environment. Selected eight domestic green algae were Chlamydomonas reinhardtii, Desmodesmus subspicatus (=Scenedesmus subspicatus), Scenedesmus abundans, Scenedesmus acutus, Scenedesmus quadricauda, Podohedriella falcata (=Ankistrodesmus falcatus), Pseudokirchneriella subcapitata (=Selenastrum capricornutum), and Chlorella vulgaris. Forty four test methods were collected from the standard test ones, government reports, SCI papers and Korean research papers. P. subcapitata and D. subspicatus are the most common test species recommended by the standard test methods. Initial cell density and dilution water were the main differences among the test methods we collected. We proposed the suitable ecotoxicity test methods based on domestic green algae in Korea. This study could be a fundamental basis to establish the ecotoxicity test methods by green algae distributed in Korea.

• Korean Journal of Ecology and Environment
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• v.40 no.3
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• pp.357-369
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• 2007

Water fleas are very important organisms in aquatic ecosystem because they are key constituents of food webs as either glazers or foods for predators. Daphnia magna is a representative test species and it has been extensively used in ecotoxicity evaluation. However, Daphnia magna has not been found yet in Korean water environment. There are limitations of using the foreign species such as Daphnia magna to reflect domestic situations in Korea. Consequently it is mandatory to find domestic species suitable for bioassay, and to develop corresponding toxicity test methods using the domestic species. In this study, we widely collected the domestic and foreign toxicity test methods performed by using domestic water fleas in Korea. The 58 test methods were collected from the standard methods (OECD, US EPA, ASTM), government reports, SCI papers and domestic papers. Ten domestic water fleas selected were Bosmina longirostris, Ceriodaphnia dubia, Ceriodaphnia reticulata, Daphnia obtusa, Daphnia puex, Moina macrocopa, Moina micrura, Simocephalus mixtus, Simocephalus serrulatus, and Simocephalus vetulus. We suggested the domestic ecotoxicity test methods using domestic water fleas in Korea through providing a range of test conditions, and future directions for toxicity test using water flea. This study could be a useful basis for establishing the aquatic toxicity test methods with domestic organisms in Korea.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.114-115
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• 2003

양식 생물에게 있어 용존산소는 호흡에 필수적이며, 양식 시설 내에서 적절한 용존 산소량을 유지하는 것은 생산량을 좌우하는 가장 중요한 요인이며, 어종, 수온, 광주기, 나이와 크기, 어류의 행동과 먹이 공급 및 환경 조건에 따라 달라진다. 이 중 같은 종 내에서 선발육종된 개체와 일반 양식 종 그리고 이들 계통간 잡종에 대한 대사 효율 즉, 용존산소 소비율의 차이에 관한 연구는 거의 없다. 따라서 본 실험에서는 한국해양연구원에서 선발육종 해 온 참돔과 일본 양식산 참돔 및 이들의 교배 자손들을 대상으로 수온과 광주기 변화에 따른 용존산소 소비율을 조사하였다. 실험어는 일본 양식산인 TPN 교배구 자손과 한국해양연구원 선발육종산인 KORDI F4 교배구 자손, JPN♀×KORDI F4♂ 그리고 KORDI F4♀×JPN♂ 교배구 자손을 대상으로 실시하였다. 체중이 각각 52.0±0.6 g(JPN 교배구; 그룹 1), 52.3±0.7 g(JPN♀×KORDI F4♂; 그룹 2), 51.7±0.4 g(KORDI F4♀ × KORDI F4♂; 그룹 3) 그리고 52.1±0.7 g(KORDI F4♀ × JPN♂; 그룹 4)인 참돔 치어를 각각 5마리씩 3반복 수용하여 실험에 이용하였다. 실험기간 동안 사육수의 pH는 8.1±0.1, 염분도는 34.0±0.5‰로 유지하였으며, 실험 장치는 김(1999)이 고안한 순환 시스템을 이용하였다. 수온은 각각 15℃, 20℃ 및 25℃로 변화시켰으며 각 수온 조건에서 광주기를 24L:0D, 12L:12D 그리고 0L:24D로 변화시켜 매 조건마다 용존 산소 소비량을 측정하였다. JPN 교배구인 그룹 1의 경우, 수온 15℃일 때 산소 소비량은 170.35∼266.29 mg O₂/kg fish/hr의 범위를 보였으며 수온 20℃와 25℃일 때 각각 236.76∼307.37 mg O₂/kg fish/hr와 346.96∼459.30 mg O₂/kg fish/hr 범위를 보여 수온 상승에 따라 산소 소비량 역시 증가하는 것으로 나타났다. 그룹 2의 경우, 수온 15℃일 때 산소 소비량은 162.01∼279.51 mg O₂/kg fish/hr의 범위를 보였으며 수온 20℃와 25℃일 때 각각 303.48∼342.72 mg O₂/kg fish/hr와 447.18∼528.45 mg O₂/kg fish/hr 범위를 보여 그룹 1과 마찬가지로 수온 상승에 따라 산소 소비량 역시 증가하는 것으로 나타났다. 그룹 3의 경우, 수온 15℃일 때 산소 소비량은 170.11∼220.98 mg O₂/kg fish/hr의 범위를 보였으며 수온 20℃와 25℃일 때 각각 262.62∼282.27 mg O₂/kg fish/hr와 302.24∼415.73 mg O₂/kg fish/hr 범위를 보여 그룹 1, 2와 유사한 경향을 보였다. 그룹 4의 경우, 수온 15℃일 때 산소 소비량은 156.03∼214.49 mg O₂/kg fish/hr의 범위를 보였으며 수온 20℃와 25℃일 때 각각 238.40∼274.28 mg O₂/kg fish/hr와 379.93∼430.97 mg O₂/kg fish/hr 범위를 보여 수온 상승에 따라 산소 소비량 역시 증가하는 것으로 나타나, 그룹 1, 2와 유사한 경향을 보였다. 모든 실험구에서 수온 상승과 함께 산소 소비량이 증가하는 것으로 나타났으며 가장 높은 산소 소비량은 그룹 2에서 관찰되었다. 실험 결과 JPN 계통의 암컷을 사용하여 생산된 그룹 1과 2의 산소 소비량이 KORDI F4 계통의 암컷을 사용한 그룹 3, 4보다 대체적으로 높은 것으로 나타났다. 연속적인 명기 조건인 24L:0D에서 수온 15℃의 경우 그룹 1, 2, 3 그리고 4의 시간당 산소 소비량은 각각 266.29 mg O₂/kg fish/hr,279.51 mg O₂/kg fish/hr, 220.98 mg O₂/kg fish/hr 그리고 214.49 mg O₂/kg fish/hr으로 나타났고, 명기와 암기가 동일한 조건인 12L:12D에서는 각각 192.20 mg O₂/kg fish/hr, 258.03 mg O₂/kg fish/hr, 192.76 mg O₂/kg fish/hr 그리고 170.40 mg O₂/kg fish/hr로 나타났다. 또한 연속적인 암기 조건인 OL:24D 조건에서는 각각 170.35 mg O₂/kg fish/hr, 162.01 mg O₂/kg fish/hr, 170.11 mg O₂/kg fish/hr 그리고 156.03 mg O₂,/kg fish/hr로 나타났다. 그리고 수온 20℃와 25℃에서도 그룹간의 변화는 이와 비슷한 경향을 보였다. 연속 명기 조건인 24L:0D에서의 산소 소비량이 명기와 암기가 동일한 조건인 12L:12D와 연속 암기 조건인 0L:24D에서의 산소 소비량 보다 대체적으로 높은 것으로 나타났으며, 그룹 2의 연속 명기 조건에서 528.45 mg O₂/kg fish/hr으로 가장 높게 나타났다. 그룹 4에서는 대사량이 점점 더 높아지는 수온 상승과 함께 연속 명기 조건과 더불어 12L:12D 조건에서의 산소 소비량이 크게 증가하는 것으로 나타났다.

• Journal of Food Hygiene and Safety
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• v.20 no.1
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• pp.22-35
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• 2005

A survey was conducted to investigate the perception of food sanitation officers toward the Genetically Modified Foods. They were mainly from Regional Agencies of KFDA, City/Province office, and National quarantine station. Some of them were professors of university and researchers of research institute. Most of respondents had experiences of hearing or reading GM foods (over $95\%$) and over $90\%$ of respondents much needed the label of GM foods. Although some of officers of city/province office and national quarantine station showed less knowledge than other respondent groups, most of respondents had basic knowledge about biology. The frequency of respondents worked over 20 years and worked in the general administration was higher than that of other groups in the question of unsafe of GM foods. The answer frequency of careless treatment of foods was highest in the question of risk factor, and the frequency of GM foods was lowest ($4.4\%$). It was concluded that food sanitation officers had positive opinion about GM foods, but there were some differences in the knowledge among agencies. Therefore, it is necessary more educations and informations are needed for food sanitation officers.

• Korean journal of food and cookery science
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• v.19 no.6
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• pp.783-793
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• 2003

The effects of various kinds of starch (mungbean, potato, corn and a blend of potato and com starch) on the quality characteristics of muhwakwa-pyun were studied according to the storage duration. The sweetness, pH and total acidity of muhwakwa extract were analyzed, as were the Visco/Amylograph viscosity profile and DSC thermodynamic characteristics of starches. For quality characteristics, color difference, instrumental texture characteristics and sensory characteristics were compared. The sweetness of muhwakwa extract was satisfactory but the total acidity was low for Kwa-pyun manufacture. Therefore, its pH was adjusted to 3.4 by adding 10％ citric acid with an amount of 1％ of total liquid. From the Visco/Amylograph viscosity profile and DSC thermodynamic characteristics of starches, a blend of potato and com starch was found to be easy to cook and form a gel but a little more resistant than the other starches in terms of retrogradatin. Lightness (L) and yellowness (b) of Kwa-pyun increased while its redness (a) decreased by the storage. The color became whiter and the clarity decrease regardless of the kinds of starch, which became obvious after 3 days of storage. The hardness, springiness, adhesiveness and cohesiveness tended to decrease with storage, which was weak in a blend starch. The overall acceptability of Kwa-pyun made with a blend of potato and com starch was evaluated as being the best among the samples tested. Therefore, for traditional kwa-pyun manufacture manufacture mungbean starch might be replaced with a blend of potato and corn starch which is less expensive and easily available in the market nowadays.

• Journal of Conservation Science
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• v.25 no.3
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• pp.283-291
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• 2009

Methyl Bromide that was used as fumigation gas turned out to be the substance of destroying the ozone layer. For that reason, at the Montreal Protocol in 1987 the use of methyl bromide was forbidden starting 2005 in the advanced country. Also according to the 2007 Bali Protocolly methyl bromide is expected to be forbidden and therefore the purpose of this study is to find out the effects of substitution fumigation gas (Ethylene Oxide+HFC 134a, Methyl Iodide, Cyanogen and Argon) on the metal(silver, copper and iron), wood(oregon pine), pigment(yellow, red, blue, white and black), textile(hemp, ramie, jute, silk1 and silk2 / indigo, safflower and cork) and paper. After the fumigation test, ethylene oxide+HFC 134a did not have changes in the weight and color of the material itself before and after the experiment. On exterior alteration, color change occurred partly on paper and metal. Also, in most materials color change extent was 0.5 to 1.5 on the average and showed scanty difference. The materials after the fumigation test with methyl iodide did not show weight changes after the test. However, color changes more than 1.0 was shown in most of the materials especially in dyed textile material. In blue pigment, the discoloration on the surface could be seen by naked eyes. Fumigation test with cyanogen gas did not show weight changes and discoloration is more than 1.5 before and after the test. The weight changes of test materials with the argon gas was decreased about 3 to 6%. It can be observed that discoloration on paper was generated. Color changes can be seen on jute dyed with safflower and cork for two weeks with argon gas and the extent was 6.3 and 6.0.

• Journal of Environmental Impact Assessment
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• v.23 no.6
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• pp.492-504
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• 2014

Feral pigeon (Columba livia) has been known as a good indicator for accumulations of chemical pollutants in urban areas. However, it is against the animal rights to kill the indicator species in order to monitor pollutants accumulations in wild birds. Eggs and feathers of birds, therefore, have been used as non-invasive monitoring materials. Even though eggs are a good indicator for accumulations of lipophilic pollutants, but unsuitable for some heavy metals such as lead and cadmium because bird's ovary builds a sort of barrier to inhibit higher accumulations of some heavy metals in the eggs. Therefore, feathers instead of eggs have been used as a non-invasive indicator for accumulations of heavy metals. However, there are few studies of heavy metal accumulations of feral pigeon in Korea. In this study, we characterized the characteristics of heavy metal accumulations of feathers in relation to internal organs (bloods, viscera and bones) in feral pigeons between two sites (Hangang Park representing urban area and Hampyeong Park for rural area). The samples from the Hangang Park showed significantly higher lead (Pb) concentrations in the blood, liver and bone than those from Hampyeong Park. The Pb concentration in the feathers was also significantly higher at Hangang Park than at Hampyeong Park. The analytical result for the breast, wing and tail feathers, and the internal organs (blood, lung, liver, kidney and bone) indicated that the Pb concentrations in the feathers were significantly positively correlated with the levels in the kidney and bone. Overall, feathers of feral pigeon may be candidate for bioindicator to monitor for Pb accumulations in urban areas.

• Journal of the Korean Society of Food Culture
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• v.14 no.3
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• pp.259-269
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• 1999

The purpose of this study was to investigate the eating behavior, food preference and nutrition knowledge according to the body style of male college students. The subjects were 219 male students enrolled in University, Taegu. The data was collected by using a survey questionnaire and an anthropometric measurement. The results were summarized as follows: The mean height, weight and BMI of subjects were $173.8{\pm}8.7cm,\;65.8{\pm}7.9Kg,\;and\;21.9{\pm}2.4$, respectively. The subjects were divided into three groups according to the BMI: underweight group(<20); normal group(20-25); and overweight group(>25). One third of the subjects preferred to gain weight(33.8%); 47 subjects preferred to lose weight(21.5%); and the rest of the subjects satisfied their physique(44.7%). Most of subjects considered 'supper' as the most important meal during a day. Among the food items, the most frequently and evenly ingested item by subjects was 'Kimchi' : 'Ham and sausage' was the least preferred food among subjects. There was no significant difference in preference among three groups and almost half of the subjects had irregular meal time because of busy schedule. The preference for meat showed high score among underweight group; on the other hand, the preference for fruits and vegetables showed high score among overweight group. Usually subjects gained nutrition knowledge through media including newspaper, magazine and TV. There was no significant difference of the nutrition knowledge score among three groups. Based on these results, nutrition education program for the college student should be arranged in the classes.

• Journal of Life Science
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• v.23 no.3
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• pp.426-431
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• 2013

With the objective of developing a functional food source, we established optimal processing conditions for the larvae of Allomyrina dichotoma, which have been used in traditional medicine to treat hepatic disorders in Korea. Without suitable processing, the larvae are difficult to consume as a food because of their disgusting taste and smell; moreover, in this form they might be a potential microbial hazard. In this study, we investigated the effect of feeding material, sterilization, and powdering after freeze-drying on the food quality of the larvae of A. dichotoma and on cytotoxicity against Raw 264.7 cells. Three to five days feeding with the sawdust from discarded oak-trees is sufficient for the breeding process. The sawdust was sterilized by vapor for five minutes. Sterilization of the larvae at a high temperature ($115^{\circ}C$ for 5 min, 0.9 $kgf/cm^2$) is necessary to eliminate pathogenic bacteria and fungi. The results of the cytotoxicity assay showed no toxicity in the prepared extract from larvae of A. dichotoma. In addition, to prepare the larvae for human consumption, various feeds were used and the smell, color, and taste were evaluated. Our results suggested that larvae of A. dichotoma could be developed as food source when a suitable processing method is established.

• Journal of the Korean Society of Food Culture
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• v.11 no.4
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• pp.455-463
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• 1996

A survey was conducted to investigate the perceptions of health and foods, and the food habits among the elderly men who came to the Tap-Gol park on a daily basis. A total of 253 subjects were selected for this study. The data was analyzed through the $X^2-test$, Duncan's multiple range test and Pearson Correlation by the use of SAS program. The results were as follows. The majority of the subjects were in their 70's. 56.1% of them lived with their sons' families, 9.1% lived alone, and 27.3% lived with a spouse. 32% of the subjects were living with the expense less than 50,000 won per month. Although some were suffering from such diseases as arthritis, indigestion, and hypertension etc., the subjects were generally in good health. Their dependance on dietary supplements were insignificant. Most of the subject had a common-sensible notion as to health. They put emphasis on the three factors for the maintenance of good health: a balanced diet, a peace of mind and exercising. With regard to the food habits, the majority were fair in general. The subject living with family had more regular meals than the single people did. 41.5% of the subjects responded that they had irregular meals, mainly for lunch due to a poor appetite or a financial problem. The respondents cited protein food, milk, fruit, sea weeds and food cooked with oil as conducive to good health. But what they consumed did not match what they thought was good, particularly milk. The results of this study lead to the suggestion that Korea need to develop such a lunch program for the elderly as is practiced in the U.S., through which dietary motivation can be stimulated and a low-priced and balanced diet offered at least for one meal a day.