• Microbiology and Biotechnology Letters
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• v.18 no.1
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• pp.103-108
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• 1990

The enzymatic studies on the methylglyoxal metabolism in yeast and bacterial cells indicated that organisms are equipped with the common and manifold systems for the detoxification of methylglyoxal. Among these systems, the glyoxalase I is the most important route for methylglyoxal detoxification. The molecular structure of glyoxalase I is apparently distinct from the enzyme sources, and zinc ion is an essential cofactor in enzyme activity. The gene for Pseudomonas putida glyoxalase I functioned as a scavenger of methylglyoxal and regulated the cell size of the bacterium. Comparison of the nucleotide sequence of the P. putida glyoxalase I gene with the N-terminal amino acid sequence of the purified enzyme revealed that the N-terminal methionine residue was removed after translation. Possible physiological role of glyoxalase I was also discussed.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.20 no.6
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• pp.1074-1080
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• 2016

Big data analysis refers the ability to store, manage and analyze collected data from an existing database management tool. Thus, meta-analysis is a statistical integration method that delivers an opportunity to overview the entire result of integrating and analyzing many quantitative research results. Commonly, factors of metabolic syndrome can be defined as abdominal obesity, systolic blood pressure, diastolic blood pressure, triglycerides, and high density lipoprotein cholesterol. In this meta-analysis, we concluded that the path between pre and post of the fasting blood glucose had the largest effect size of (r = -.324). Therefore, the effect of soybean food intervention showed an explanatory power of 10%. The second biggest effect size (r = .256) was found the path between pre and post in the waist circumference. Unfortunately, soybean food intake showed no improvement on abdominal obesity. Thus, we present the theoretical and practical implications of these results.

• Applied Biological Chemistry
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• v.10
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• pp.47-61
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• 1968

Sweetpotato infected by Soft-rot, Rhizopus nigricans, has been investigated for its biochemical changes. The results of the present experiment are summarized as follows: 1. From the Soft-rotted tuber, nineteen Ehrlich's positive substances and three fluorescent compounds which had not been found in a healthy tuber were detected with thin layer chromatography. 2, Ipomeamarone and umbelliferone, each of which are predominating substances among the Ehrlich's positive and fluorescent compounds, were isolated by silica gel column chromatography and studied for their identities. 3. Time course biosynthesis of the metabolites was studied and discussed for their possible biosynthetic pathways. 4. Among the metabolites, ipomeamarone and ipomeamarone-like substances have enzyme: inhibitive action against ${\alpha}$-amylase and probably other enzymes; the inhibition type was determined as the uncompetitive one. 5. Ipomeamarone obtained from soft-rotted sweetpotato was proved to have an uncoupling action as 2, 4-dinitrophenol.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.10a
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• pp.143-146
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• 2015

A meta-analysis is a statistical integration method that delivers an opportunity to overview the entire result of integrating and analyzing many quantitative research results. This study will find meaningful mediator variables for criterion variables that affect between pre and post in metabolic syndrome studies, on the basis of the results of a meta-analysis. We reviewed a total of 36 studies related metabolic syndrome published in Korean journals between 2000 and 2015, where a cause and effect relationship is established between variables that are specified in the conceptual model of this study. In this meta-analysis, the path between pre and post in waist circumference showed the biggest effect size (r = .420). The second biggest effect size (r = -.402) was found the path between pre and post in high density lipoprotein cholesterol. By the way, one the smallest effect size (r = .234) was obtained the path between pre and post in diastolic blood pressure. Thus, we present the theoretical and practical implications of these results and discuss the differences between these results through a comparative analysis with previous studies.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.11
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• pp.2590-2596
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• 2015

A meta-analysis is a statistical integration method that delivers an opportunity to overview the entire result of integrating and analyzing many quantitative research results. This study will find meaningful mediator variables for criterion variables that affects between pre and post in the metabolic syndrome studies, on the basis of the results of a meta analysis. We reviewed a total of 36 studies related the metabolic syndrome published in Korean journals between 2000 and 2015, where a cause and effect relationship is established between variables that are specified in the conceptual model of this study. In this meta-analysis, the path between pre and post in the waist circumference showed the biggest effect size (r = .420). The second biggest effect size (r = -.402) was found the path between pre and post in the high density lipoprotein cholesterol. By the way, one the smallest effect size (r = .234) was obtained the path between pre and post in the diastolic blood pressure. Thus, we present the theoretical and practical implications of these results.

• Journal of Life Science
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• v.33 no.11
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• pp.944-949
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• 2023

The purpose of this study was to identify conserved metabolic pathways and conserved genes in 122 archaeal species. Using the Clusters of Orthologous Groups of Proteins (COG) database of conserved genes, we analyzed whether 122 species had 63 COG metabolic pathways, the 822 COGs that compose them, and a total of 4,877 COGs. Archaeal ribosomal proteins were the most conserved in metabolic pathways. 46 COGs in seven COG pathways among 63 COG pathways and 20 COGs in others were conserved in 122 species. Some genes involved in cell wall and extracellular matrix synthesis, replication, transcription, translation, and protein metabolism were common to all 122 species. When the distance value of the phylogenetic tree was analyzed at the phylum level or class level, the average was the lowest at the class Halobacteria of the phylum Euryarchaeota. Standard deviation was high for the class Nitosospharia of the phylum Thaumarchaeota, the unclassified members of phylum Thaumarchaeota, the class Halobacteria of the phylum Euryarchaeota, the class Thermoprotei of the phylum Crenarchaeota, and other archaea. Furthermore, the phylogenetic tree analysis revealed six commonalities. The results of this study, along with data on conserved genes, could be used for drug development and gene selection for strain improvement.

• Development and Reproduction
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• v.12 no.1
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• pp.19-30
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• 2008

Proper development of fertilized oocyte to blastocyst is a key step in mammalian development to implantation. During development of preimplantation embryos, the mammalian embryo needs supply the energy substrate for keep viability. Usually mammalian oocyte get substrate especially energy substrate from oviduct and uterus, because it does not store much substrate into cytoplasm during oogenesis. Carbohydrates are known as a main energy substrate for preimplantation stage embryos. Glucose, lactate and pyruvate are essential component in preimplantation embryo culture media and there are stage specific preferences to them. Glucose transporter and $H^+$-monocarboxylate cotransporter are a main mediator for carbohydrate transport and those expression levels are primarily under the control of intrinsic or extrinsic factors like insulin and glucose. Other organic substances, amino acids, lipids and nucleotides are used as energy substance and cellular regulation factor. Though since 1960s, successful development of fertilized embryo to blastocyst has been accomplished with chemically defined medium for example BWW and give rise to normal offspring in mammals, the role of metabolites and the regulation of intermediary metabolism are still poorly understood. Glucose may permit expression of metabolic enzymes and transporters in compacting morula, capable of generating the energy required for blastocyst formation. In addition, it has been suggested that the cytokines can modulate the metabolic rate of carbohydrate in embryos and regulate the preimplantation embryonic development through control the metabolic rate. Recently we showed that lactate can be used as a mediator for preimplantation embryonic development. Those observations indicate that metabolites of carbohydrate are required by the early embryo, not only as an energy source, but also as a key substrate for other regulatory and biosynthetic pathways. In addition metabolites of carbohydrate may involve in cellular activity during development of preimplantation embryos. It is suggested that through these regulation and with other regulation mechanisms, embryo and uterus can prepare the embryo implantation and further development, properly.

• Journal of Plant Biotechnology
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• v.33 no.3
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• pp.161-169
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• 2006

Plant metabolomics is a plant biology field for identifying all of the metabolites found in a certain plant cell, tissue, organ, or whole plant in a given time and conditions and for studying changes in metabolic profiling as time goes or conditions change. Metabolomics is one of the most recently developed omics for holistic approach to biology and is a kind of systems biology. For holistic approach, metabolomics frequently uses chemometrics or multivariate statistical analysis of metabolic profillings. In plant biology, metabolomics is useful to determine functions of genes often in combination with DHA microarrays by analyzing tagged mutants of the model plants Arabidopsis and rice. This review paper attempted to introduce basic concepts of metabolomics and practical uses of multivariate statistical analysis of metabolic profiling obtained by $^1$H HMR and Fourier transform infrared spectrometry.

• The Journal of the Korea Contents Association
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• v.21 no.8
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• pp.662-674
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• 2021

Metabolic syndrome is closely associated with cardiovascular disease, there is increasing attentions in prevention of metabolic syndrome through prediction. The aim of this study was to systematically review the literature by collecting, analyzing, and synthesizing articles of predicting metabolic syndrome in Koreans. For systemic review, data search was conducted on Global journals Pubmed, WoS and domestic journals DBPia, KISS published in 2011-2020 year. Three keyword 'Metabolic syndrome', 'predict', and 'korea' were used for searching under AND condition. Total 560 articles were searched and the final 22 articles were selected according to the data selection criteria. The most useful variable was WHtR(AUC=0.897), most frequently used analysis method was logistic regression(63.6%), and most accurate analysis method was XGBOOST(AUC=0.879) for predicting metabolic syndrome. Prediction accuracy was slightly improved when sasang constitution types was used. Based on the results of this study, it is believed that various large-scale longitudinal studies for the prediction and management of the Metabolic syndrome in Korean should be followed in the future.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.6
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• pp.389-393
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• 2013

The purpose of this study is to investigate the biodegradation characteristics of the xylene by BTEX-degrading bacteria, Pseudomonas putida BJ10, isolated from oil-contaminated soil and bio-degradation pathway of the xylene. The removal efficiencies of o, m, p-xylene in mineral salts medium (MSM) by P. putida BJ10 were 94, 90 and 98%, respectively for 24 hours. It shows clear difference compared with the control groups which were below 3%. The removal efficiencies of BTEX by P. putida BJ10 in gasoline-contaminated soil were 66% for 9 days. They were clearly distinguished from the control groups (control and sterilized soil) which were 32 and 8%. 3-methylcatechol and o-toluic acid were detected after 6 and 24 hours during the o-xylene biodegradation pathway. Therefore, we confirmed o-toluic acid as the final metabolite. And intermediate-products were somewhat different with previously published studies of the transformation pathway from o-xylene to 3-methylcatechol.