• Korean Journal of Plant Tissue Culture
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• v.28 no.1
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• pp.33-36
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• 2001

The experiments were conducted to establish the in vitro culture system of apple rootstock M.9. The meristem tissue of M.9 were pre-treated in antiox: dant solution containing 100 mgL$^{-1}$ ascorbic acid and 150 mgL$^{-1}$ citric acid for 30 minutes, transferred to the MS liquid medium added with 0.1 mgL$^{-1}$ IBA, 0.5 mgL$^{-1}$ GA, and 30 gL$^{-1}$ sucrose, which shaked by 50 rpm for 2 weeks, and then, cultured in same composition of MS agar medium. This treatment stimulated shooting from the tissue, the most favorably, compared with other treatments. All young shoots produced normal roots when they were shake-cultured on the 1/2MS liquid medium added with 0.5 mgL$^{-1}$ IBA, 30 gL$^{-1}$ sucrose and 1,000 times diluted solution of Hormex by 50 rpm for one week, and subsequently transferred to the 8 gL$^{-1}$ agar medium of the same composition as pre-culture medium minus Hormex.

• Korean Chemical Engineering Research
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• v.55 no.1
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• pp.74-79
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• 2017

In this study, the optimization of several factors for Chlorella saccharophila cultivation was investigated. The studied factors were medium type, culture type, inoculum size, sugar/nitrogen source type and concentrations. As a result, the optimized conditions for C. saccharophila cultivation were found to be the best at 3% (v/v) inoculum, 30 g/L glucose and 0.95 g/L $NaNO_3$ under mixotrophic culture. Under the optimized condition, the content of oil was high at 12 day, whereas, the amount of biomass and chlorophyll were high at 10 day.

• FLOWER RESEARCH JOURNAL
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• v.17 no.2
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• pp.114-120
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• 2009

The most effective conditions of in vitro culture were studied for mass propagation of Pteris cretica 'Wilsonii'. Spores of the species germinated within 7 weeks. The greatest proliferation was obtained with Knop and Hyponex media, but growth was more effective in Hyponex medium. MS medium induced necrosis of prothalli in all strength of nitrogen and sucrose except in case of 0% sucrose. Hyponex medium supplemented with 1% sucrose and 0.6% agar promoted propagation and growth of prothalli. In Hyponex medium, optimal inoculation method was homogenization, but in MS medium dividing colonies of prothalli was more effective. Culturing on solid medium was more effective than liquid culture method. Liquid culture induced necrosis of prothalli. Shaking cultured prothalli showed good growth, but propagation was inhibited compared to those cultured on solid medium.

• Journal of Plant Biotechnology
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• v.29 no.1
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• pp.37-40
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• 2002

Sweet potato is a crop vegetatively propagated by vine cuttings, an ineffective method for maintaining pathogene-free stock plants. As an alternative method, single-node cultures of virus-free plantlets derived from apical meristem in sweet potato (cv. Yulmi) was examined. Effective pH range, sugar concentration and nodal order were investigated to establish an in vitro mass propagation system with high quality virus-free stock plantlets to farmhouse. Although the plantlets grew at wide range of pH, the most effective pH of the medium was 4.8 in single-node cultures. High sugar concentration of 60∼80 g/L resulted in increased growth response in shoot length, root length, number of node, leaf area and fresh and dry weight of shoot and root, whereas reducing sugar contents below 6% was showed reduced growth response. The first node including meristem tip was the best for the rapid growth of plantlets and the other nodes also showed a very similar growth response. Uniform plantlet can be obtained massively at the same time by culture of single node except for the first node including meristem tip. In conclusion, the most effective pH range and sugar concentration of medium for the growth of plantlets via single-node cultures was 4.8, 60∼80 g/L respectively. The first node was the best for the rapid propagation of plantlets in nodal order.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.5
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• pp.379-386
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• 2021

This study was undertaken to develop a cascade-type continuous culture system (CCCS) that combines both ICT and biotechnology (BT), for the mass production of microalgae. This system is capable of maintaining the essential culture conditions of pH, temperature, carbon dioxide, and illuminance control, which are key parameters for the growth of microalgae, and is economical for producing microalgae regardless of the season or location. It has the added advantage of providing stable and high productivity. In the current study, this system was applied to culture microalgae for 71 days, with subsequent analysis of the experimental data. The initial O.D. of the culture measured from incubator 1 was 0.006. On the 71st day of culture, the O.D.s obtained were 0.399 (incubator 1), 0.961 (incubator 2), 0.795 (incubator 3), and 0.438 (incubator 4), thereby confirming the establishment of continuous culture. Thus, we present a smart-farm based on ISMC (in-situ monitoring and control) for a mass culture method. We believe that this developed technology is suitable for commercialization, and has the potential to be applied to hydroponics-based cultivation of microalgae and cultivation of high-value-added medicinal plants as well as other plants used in functional foods, cosmetics, and medical materials.

• Journal of Life Science
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• v.14 no.1
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• pp.126-130
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• 2004

This study was carred out to get the basic conditions for the mycelial growth of Morchella esculenta in shaking flask culture. The optimal temperature and initial pH of mycelial growth of Morchella escuzenta were 25$\pm$1$^{\circ}C$ and 6.5, respectively. The optimal medium was BG medium. Among the carbon sources tested, fructose was favorable for the mycelial growth and optimal fructose concentration was 5.0% (w/v). As nitrogen sources, peptone and NH$_4$Cl appeared to be favorable and optimal concentration was 4.0% [(w/v), ratio of 1:1].

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.41-42
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• 2001

• 한국생태학회:학술대회논문집
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• 1999.05a
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• pp.125.1-125.1
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• 1999

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.05a
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• pp.255-256
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• 2002

• 발명특허
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• v.36 no.10
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• pp.65-68
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• 2011