• Applied Chemistry for Engineering
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• v.32 no.1
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• pp.10-14
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• 2021

mCherry is one of the well-understood red fluorescent proteins which has a similar tertiary structure as GFPs, but pH resistant due to the lack of hydrogen bond network. Whereas mCherry-I202T showed far-red fluorescence and also pH sensitive property because of the additional hydrogen bond formed by substituting Ile of 202 amino acid sequence on mCherry with Thr. In order to verify the pH sensitive characteristic of mCherry-I202T owing to the extension of hydrogen bond, UV-vis spectrum was measured over the range of acidic to basic pH. We also demonstrate further possibilities of applying mCherry-I202T as a pH sensor.

• Journal of Life Science
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• v.7 no.3
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• pp.161-166
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• 1997

HIV-1 Rev protein plays an important role in regulating the expression of viral structural proteins. It allows the nuclear export and accumulation of unspliced and partially spliced viral mRNA in the cytoplasm. The Rev-responsive element RNA, present in the env gene, forms a higly ordered RNA secondary structure and is required for the Rev-mediated mRNA export. For this process to complete factor(s) are strongly suggested. From our experiments of electrophoretic mobility shift, UV-crosslinking and SDS/PAGE, RRE RNA was found to be recognized to several nuclear factors such as 36/37, 56, 41. 76, 150 kD proteins in the order of reactivity. Among them, 36/37 and 56 kD proteins are more reactive upon a brief UV treatment (5 min) and more persistent in the presence of high amount of nonspecific competitor, heparin. Certain nuclear protein9s) seemed to recognize the RRE RNA structure in competition with Rev to gel mobility shift assay.

• Journal of the East Asian Society of Dietary Life
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• v.9 no.2
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• pp.161-168
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• 1999

This study was conducted to investigate the effects of Plantago asiatica var. densiuscula Pilg. water extracts(PWE) on fat metabolism of rats and prevention to cardiovascular disease. Male Spague-Dawley rats were divided into five groups consisting of the control group(N), $CCl_4$(T), Plantago asiatica var. densiuscula Pilg.(P), Plantago asiatica var. densiuscula Pilg. after injection of $CCl_4$(TP) and $CCl_4$ after injection of Plantago asiatica var. densiuscula Pilg.(PT) at the level of 0.5%. After 6 weeks of feeding serum lipid levels were measured for experimental rats and analyzed enzyme activity(EA). Rats were divided into five groups of 6 animals. The enzyme activities of ALT, AST, LDH and ALP increased by administration of $CCl_4$ group and decreased by Plantago asiatica var. densiuscula Pilg. water extracts. Total cholesterol and LDL-cholesterol in serum increased by administration of $CCl_4$ group and decreased by Plantago asiatica var. densiuscula Pilg. water extracts. And, HDL-cholesterol decreased by $CCl_4$ group and significantly increased by TP group(p＜0.05) Total lipid, triglyceride and phospholipid in serum decreased by P group as compared to T group(p＜0.05). Considering all results obtained throughout this experiments, it can be concluded that Plantago asiatica var. densiuscula Pilg. water extracts wald improve the liver fuction and enzyme activity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.4
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• pp.350-359
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• 1993

The effect of gibberellic acid ($GA_3) and abscisic acid (ABA) on KCl-enhanced proteolysis of senescing leaves of rice(Oryza sativa L. cv. Chilsung) was studied. Emphasis was given to their effects on KCI-enhanced efflux of amino acids and proteinase activity. When treated singly, $GA_3 affected leaf proteolysis little, while ABA increased proteolysis, the rate of amino acid efflux, and ribulose -1,5 -bisphosphate carboxylase / oxygenase (Rubisco)-degrading endoproteinase activity. An additive increase in all three parameters mentioned above was observed when leaves were treated with ABA and KCl. No such an additive effect was found when $GA_3 was treated with KCl. Both $GA_3 and ABA helped to alleviate the KCI-suppressed activity of Rubisco-degrading exoproteinases. The additive increase in proteolysis of rice leaves in the presence of both ABA and KCl could thus be ascribed to a further increase in the efflux of protein hydrolyzates and Rubisco-degrading endoproteinase activity. An increase in proteolysis was accompanied by a decrease in water absorption, and the combined treatment of ABA with KCl resulted in a further reduction of water absorption.

• Korean Journal of Agricultural Science
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• v.22 no.1
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• pp.103-109
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• 1995

The model foods were prepared by simulating mositure, protein and starch, and they were heated for 30 mins, at $80^{\circ}C$ and then cooled at $25^{\circ}C$ in water bath. Their rheological properties were investigated by the use of Brookfield wide-gap rotational viscometer at $30{\sim}60^{\circ}C$, and the rotation speed ranged from 0.6 to 6 rpm and solid content ranged from 8% to 11%, the results obtained were as follows. 1. All the model foods ($P_1S_3$, $P_2S_2$, $P_1S_1$, $P_2S_1$, $P_3S_1$, $P_4S_0$) exhibited pseudoplastic behaviors with yeild stress and were thixotropic foods which showed time - dependent structural decays, but the starch food of 8 ~ 11 % solid content did not show the flow behavior. 2. The correlation between the rheological parameters and the protein content of model foods in various moisture content did not appeared a constant relationship. 3. The change of shear stress against shear rate in high starch foods was larger than that in high protein foods and the structure at initial shear time was decayed with a quatic equation according to the Tiu's Model and structural decay was in parallel with the increase of shear rate. 4. The temperature dependency of the apparent viscosity of $P_1S_2$, and $P_2S_1$ was fully expressed by Arrhenius equation and activation energies of their food were 2.35 and $1.34Kcal/g{\cdot}mol$, respectively.

• The korean journal of orthodontics
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• v.33 no.3 s.98
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• pp.185-197
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• 2003

This study was performed to identify the characteristics of the OFC1 gene (locus: chromosome 6p24.3) in Korean patients, which is assumed to be the major gene behind the nonsyndromic cleft lip and palate. The sample consisted of 80 subjects: 40 nonsyndromic cleft lip and palate patients (proband, 20 males and females, mean age 14.2 years); and 40 normal adults (20 males and 20 females, mean age 25.6 years). Using PCR-based assay, the OFC1 gene was amplified, sequenced, and then searched for similar protein structures. Results were as follows: 1. The OFC1 gene contains the microsatellite marker 'CA' repeats. The number of the reference 'CA' repeats was 21 times, and formed as TA(CA)11TA(CA)10. But, in Koreans, the number of tandem 'CA' repeats was varied from 17 to 26 except 18, and 'CA' repeats consisted of TA(CA)n. 2. Nine allelic variants were found. Distribution of the OFC1 allele was similar between the patients and control group. 3. There was a replacement of the base 'T' to 'C' after 11 tandem 'CA' repeats in Koreans compared with Weissenbach's report. However, the difference did not seem to be the ORF prediction results between Koreans and Weissenbach's report. 4. The BLAST search results showed the Telomerase reverse transcriptase (TERT) and the Nucleotide binding protein 2 (NBP2) as similar proteins. The TERT was a protein product by the hTERT gene in the locus 5p15.33 (NCBI Genome Annotation; NT023089) The NBP2 was a protein product by the ABCC3 (ATP-binding cassette, sub-family C) gene in the locus 17q22 (NCBI Genome Annotation; NT010783). 5. In the Pedant-Pro database analysis, the predictable protein structure of the OFC1 gene had at least one transmembrane region and one non-globular region.

• Journal of Aquaculture
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• v.16 no.2
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• pp.110-117
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• 2003

We have cloned and sequenced the cDNA encoding growth hormone (GH) from pituitary poly(A)$^{+}$ RNA of red-spotted grouper (Epinephelus akaara). The cDNA of red-spotted grouper GH is 883 base pairs (bp) consisting of 21 bp of 5'untranslated region (UTR), 615 Up of an open reading frame (ORF) and 247 Up of 3'UTR. The polyadenylation signal, AATAAA, was 20 bp upsteam of polyadenylation site. Based on the nucleotide sequences, the deduced putative polypeptide contains 204 amino acids (aa), representing 17 aa of a signal and 187 aa of a mature polypeptide. The putative GH cDNA encodes a polypeptide with four cysteine residues and only one N-gly- cosylation site. Comparative sequence alignment shows that red-spotted grouper GH exhibits high similarity with its corresponding other Perciformes species GH cDNAs.

• The Magazine of the IEIE
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• v.29 no.3
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• pp.58-70
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• 2002

약 1그램의 DNA는 10/sup 21/개의 DNA 염기를 가지며 이것은 10억 terabits의 정보저장 능력에 해당한다. 분자정보처리기술(molecular information processing technology)은 DNA나 단백질과 같은 거대분자의 초미세구조, 초고집적도, 초에너지 소비특성에 기반하여, 용액상에서 아보가드로수(6×10/sup 23/) 규모의 분자들이 3차원 화학 반응에 관여하는 초병렬분산 정보 저장 및 처리 능력을 활용하는 차세대 IT 기술이다. 이는 또한 생체분자의 특성을 나노수준에서 활용하는 정보기술이라는 점에 있어서 BT+NT+IT의 융합기술이며 그 기술적 산업적인 파급 효과가 아주 큰 특징이 있다. 본고에서는 현재 분자정보 처리 기술의 원리 및 문제점을 설명하고 이의 해결을 위한 5가지 핵심 요소 기술을 도출한다. 최근 세계적인 연구 동향을 살펴보고, 국내의 기술 기반 및 국제 경쟁력을 분석하며, 궁극적인 경제성 확보를 위한 산업화 방안을 단기(3-5년), 중기(5-10년), 장기(10-20년)의 3단계로 나뉘어 제안한다.

• The Korean Journal of Food And Nutrition
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• v.23 no.3
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• pp.405-410
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• 2010

The effect of microwave treatment on Korean ginseng was studied by measuring the changes in moisture, crude lipid, crude ash, crude protein, total dietary fiber and saponin contents, as well as changes in density, color and microstructure. Korean ginseng was treated with 100 or 200 watts of microwaves for 1 or 3 hrs, respectively, followed by drying using an oven at $60^{\circ}C$ for 96 hrs. The moisture contents decreased to 13.12~10.77% from an initial 76.26%. The amounts of lipid and ash were reduced in proportion to the time of microwave treatment and level of microwave power. The amount of protein in ginseng after microwave treatment did not significantly change. The amount of total dietary fiber increased after microwave treatment and the color of dried ginseng became dark. The amounts of ginsenoside-$Rb_1$, $Rb_2+Rb_3$, Rc, Rd, Re, Rf, $Rg_1$, $Rg_2+Rh_1$ and $Rg_3$ were reduced after treatment with 100 watts of microwave radiation for 1 and 3. The amounts of ginsenoside-$Rb_1$, Rd, Re, Rf, $Rg_1$, $Rg_2+Rh_1$ and $Rg_3$ after treatment with 200 watts of microwave radiation for 1 and 3 hr also reduced. On the other hand, the amounts of ginsenoside-$Rb_2+Rb_3$ and Rc after treatment of ginseng with 200 watts of microwave radiation for 1 and 3 hrs were increased.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.4
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• pp.512-517
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• 1995

Effects of nitrogen top-dressing method on quality of barley were evaluted over amount of 2 and 4 kg per 10a on volcanic ash soil in Japan under regrowth stage, productive tiller stage and heading stage. 55% milling time was longer by the amount of top- dressing, but that was not influenced by the times of top-dressing. And glassiness, whiteness, hardness and 8 minute- millng rate were not influenced by times and amounts of top-dressing. Crude protein rate of grain was increased by amount of nitrogen top-dressing, but that did not correlated with time of nitrogen top-dressing. Crude protein rate of grain was positively correlated with 55% milling time. But that did not correlated with whiteness of 8 minute-milled grain. 8 minute-milling rate was negatively correlated with whiteness of 8 minute-milled grain. There was no visible difference of protein matrix with scanning electron microscope in different nitrogen levels.