• The Science & Technology
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• v.30 no.10 s.341
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• pp.84-85
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• 1997

단백질이 합성된 후 어떻게 고유의 3차 구조를 형성하는가에 대하여 의문을 갖고 15년간 단백질폴딩연구를 계속하고 있는 대전 한국과학기술연구원 생명공학연구소의 책임연구원 유명희박사. 76년 서울대 미생물학과를 졸업한 후 미국에 유학하여 81년 버클리대학에서 박사학위를 받고 귀국한 유박사는 "앞으로 여성후학들이 공동연구로 더욱 좋은 환경 속에서 연구활동을 할 수 있는 분위기 조성을 위해 노력하겠다" 강조한다.

• Journal of Life Science
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• v.22 no.1
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• pp.7-15
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• 2012

A polyguluronate-specific lyase from Streptomyces sp. strain M3 has been previously cloned and characterized. In this study, the M3 alginate lyase gene in the pColdI vector was mutated by site-directed mutagenesis and random mutagenesis to enhance the alginate degrading activity. Six mutants were obtained: Ser25Arg, Phe99Leu, Asp142Asn, Val163Ala, Lys191Glu, and Gly194Cys. Phe99Leu and Lys191Glu mutants completely lost their alginate lyase activity, whereas the alginate degrading activity of Gly194Cys mutant increased by nearly 10 fold. The 3-D protein structure of M3 alginate lyase, which was constructed using the Swiss-Model automodeler, was also compared to the crystal structure of another alginate lyase. A mutated glycine residue was positioned between Gly193 and Tyr195 of the C-terminal conserved sequence, YFKAGXYXQ. A phenylalanine residue (at position 99) and a glycine residue (at position 194) mutated in this study were distant from the active site, but the degrading activity was strongly affected by their mutation.

• Journal of the Korean Chemical Society
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• v.67 no.2
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• pp.81-88
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• 2023

The contribution of hydrophobic residues to the protein folding reaction was studied by using HubWA variant proteins with I and L to V mutation. Folding kinetics of all V variant proteins was observed to be satisfied by a three-state on-pathway mechanism, U ⇌ I ⇌ N, where U, I, and N represent unfolded, intermediate, and native state, respectively. Three-state folding reaction was quantitatively analyzed and the free energy of folding of each elementary reactions and overall folding reaction, ΔG^o_UI, ΔG^o_IN, and ΔG^o_UN, were obtained. From the ratio of free energy difference between the variant protein and HubWA, ΔΔG^o_UI/ΔΔG^o_UN (ΔΔG^o_UI = ΔG^o_UI (variant protein) - ΔG^o_UI (HubWA) and ΔΔG^o_UN = ΔG^o_UN (variant protein) - ΔG^o_UN(HubWA)), the contribution of hydrophobic residues to HubWA folding was analyzed. The residues which are located in the hydrophobic core between α-helix and β-sheet, I3, I13, L15, I30, L43, I61 and L67, showed ΔΔG^o_UI/ΔΔG^o_UN value of ~0.5 when each of these residues was mutated to V, indicating that these residues form relatively solid hydrophobic core in the intermediate state. Residues located at the end of secondary structures and loop, I23, L69 and I36 showed ΔΔG^o_UI/ΔΔG^o_UN value below 0.4 when each of these residues was mutated to V, indicating that the region containing these residues are loosely formed in the intermediate state. V17A, L50V and L56V showed fairly high ΔΔG^o_UI/ΔΔG^o_UN value of ~0.8. Since L50 and L56 are located in the region containing long loop (residue 46 to 62), it is suggested that the high ΔΔG^o_UI/ΔΔG^o_UN value of these residues prevents the formation of aggregate at the early stage of folding reaction.

• Proceedings of the Korea Contents Association Conference
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• 2015.05a
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• pp.227-228
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• 2015

질병 치료에 대한 패러다임이 과거 치료 위주에서 조기진단 및 예방의 개념으로 전환되고 있으며 진단용 마커와 단클론 항체 제작은 핵심 기술로 부각되고 있다. 현재까지의 항원결정부위(epitope) 예측은 단백질의 1차구조인 아미노산 배열 순서를 바탕으로 추출되어 진다. 하지만 항원결정부위는 수용성의 항체와 직접 결합하기 때문에 친수성 잔기가 차지하는 비율이 높아야 하며, 면역계가 쉽게 인지할 수 있도록 노출되어 있어야하고, 긴 선상의 폴리펩티드 단백질이 3차원 구조를 형성하기 위해 회전, 유연성 등이 요구된다. 따라서 한 가지 성질 중심으로 할 경우 오류가 나올 가능성이 있다. 이를 보완하기 위하여 본 연구에서는 친수성(hydrophilicity), 극성(polarity), 파묻힘성(buried residues), 접근성(accessibility), 회전성(${\beta}-turns$), 유연성(flexibility), 굴절성(refractivity) 등을 분석한 후 통합 예측시스템을 개발하였다. 이를 검증하기 위해 고양이 백혈병 바이러스의 항원결정부위를 예측해보았다.

• Journal of Life Science
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• v.8 no.2
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• pp.223-233
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• 1998

Discovery of molecular chaperone has stimulate cell biologists and thus made it possible to re-examine the processes whereby proteins achieve and maintain their functional conformations within living cells. the term ‘Molecular chaperone’ was first coined to describe one particular protein involved in the assembly of nucleosomes, but the term has now been extended to describe the function of a wide variety of proteins that assist protein transport across membranes, folding of nascent polypeptide, the assembly and disassembly of oligomeric structures, and the recovery or removal of proteins damaged by various environmental stresses including heat shock. Progress of molecular chaperone research is still limited by the lack of 3-dimensional structural information and detailed interacts with taget proteins in the cell. However, several laboratories around the world are attempting to extend our knowledge on the functions of molecular chaperone, and such efforts seem justified to finally provide the answers to the most burning questions shortly.

• Bulletin of Food Technology
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• v.15 no.1
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• pp.52-54
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• 2002

식품에 대한 고압의 영향은 이미 19C말이나 20C초부터 연구되어 왔다. 본 논문에서는 식품의 보존기간(shelf-life)에 미치는 고압의 영향과 고압(HHP, High Hydrostatic Pressure)하에서 단백질의 3차 구조가 어떻게 변화하는 지에 대해 살펴 보고자 한다. 보통 식품가공에 적용되는 고압은 몇 초 내지 몇 분 동안에 50∼1000MPa 정도의 압력이 가해지는 것을 말한다. 소비자들이 식품을 선택할 때 가장 먼저 민감하게 느끼는 점은 식품의 색이다. 그러나 색은 가공과정이나 유통기간중에 변색이 쉽다. 따라서 색이나 풍미성분의 손실을 최소화하면서 식품의 보존기간을 늘리기 위해서 고압처리 기술이 주목을 받게 된 것이다. 식품의 색은 소비자들로 하여금 그 식품의 품질을 미리 짐작하게 하는 중요한 요소로서, 과일의 경우는 당도까지도 예측하게 한다. 그러므로 식품의 색이 소비자에게 주는 정보를 과소 평가해서는 안된다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.11
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• pp.5350-5355
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• 2012

Human tyrosinase (hTyr) catalyzes the first and rate limiting step in the biosynthesis of a skin color determinant, melanin. Although a number of cosmetic companies have tried to develop hTyr inhibitors for several decades, absence of 3D structure of hTyr make it impossible to design or screen inhibitors by structure-based approach. Therefore, we built a 3D structure by comparative modeling technique based on the crystal structure of tyrosinase from Bacillus megaterium to provide structural information and to search new hit compounds from database. Our model revealed that two copper atoms of active site located deep inside and were coordinated with six strictly conserved histidine residues coming from four-helix-bundle. Substrate binding site had narrow funnel like shape and its entrance was wide and exposed to solvent. In addition, hTyr-tyrosine and hTyr-kojic acid, a well-known inhibitor, complexes were modeled with the guide of solvent accessible surface generated by in-house software. Our model demonstrated that only phenol group or its analogs could fill the binding site near the nuclear copper center, because inside of binding site had narrow shape relatively. In conclusion, the results of this study may provide helpful information for designing and screening new anti-melanogenic agents.

• Proceedings of the Korean Information Science Society Conference
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• 2002.10c
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• pp.160-162
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• 2002

서열 시퀀싱을 통해 등장하는 원시 데이터들을 대상으로 유사한 서열과 기능 예측에 사용되는 모티프 데이터베이스들은 원시 데이터 생성 속도가 빠르게 증가함에 따라 그 중요성 또한 나날이 증가하고 있다. 그러나, 이러한 모티프 데이터베이스들은 서로 독자적으로 개발되고 발전되어 왔기 때문에 각각 서로 다른 형식의 데이터를 사용하고 있어 이에 대한 검색결과도 데이터베이스마다 서로 이질적인 형태로 제공하고 있다. 그러므로 사용자는 각 데이터베이스에서 사용하는 데이터 구조들에 대한 전반적 지식을 습득해야 할 뿐만 아니라 중복된 반복 검색 작업을 하여야 한다. 따라서, 이 논문에서는 이러한 문제 해결을 위해 독립적인 모티프 데이터베이스들의 자원을 분해하고, 합병하는 과정을 거쳐 하나의 통합된 모티프 데이터베이스를 구축하였다. 또한 데이터베이스의 각 엔트리당 단백질의 3차 구조 정보, 분류 정보, 샘플 정보의 지원을 가능케 하여 기존 검색 조건을 개선하였다. 이 데이터베이스 구축으로서 사용자는 모티프 데이터베이스 검색에 대한 streamline적인 검색이 가능할 뿐만 아니라 기존의 통합된 데이터베이스에서 지원되지 못한 구조 정보, 분류 정보 검색을 가능케 하였다.

• The Korean Journal of Food And Nutrition
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• v.15 no.4
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• pp.331-336
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• 2002

Conformation of soymilk protein was examined to obtain basic information for improved calcium intolerence of soymilk protein partially hydrolyzed with protease. Surface hydrophobicities of three proteins showed the order of SMP(soymilk protein) < SPI(soy protein isolate) < PT-SMP(protease treated soymilk protein). Total thiol group contents of SMP and PT-SMP were similar but larger than that of SPI. Reducing rate of disulfide bond in PT-SMP after 2-mercaptoethanol treatment was laster than that in SMP. And so, this result indicates that PT-SMP may be less compacting due to protease treatement. From circular dichroism result, PT-SMP showed different pattern from SMP and SPI suggesting change of secondary structure by hydrolysis. And analysis of heat denaturating property by DSC showed that denaturation enthalpy of three proteins were all small. Especially enthalpy of PT-SMP was least, and this result suggested that PT-SMP was denatured easily by heating due to less compacting structure.

• The Korean Journal of Zoology
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• v.38 no.3
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• pp.417-425
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• 1995

The present study was carried out to investigate the effect of microwave fixation in comparison with that of chemical fixation in preparing the microscopic samples. The microwave fixator was equipped with infrared-temperature sensor, and that was designed to compensate air temperature in the microwave fixator. In the microwave fixation, rat tongue was well preserved in terms of muscular fasciculus and pancreas stained by Feulgen reagents showed clear reaction products in the nucleus. Reaction products by PAS method in duodenal villi appeared specifically at the goblet cells. In electron microscopy, pancreatic cellular components such as secretory granules and collagen bundles were well preserved in both fixations. In aspect of histochemical reaction and electron microscopy, high quality was due to the protein content of microwave fixed specimen. The microwave fixation method saved total duration engaging microscopic preparation.