• Journal of Chest Surgery
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• v.39 no.6 s.263
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• pp.427-433
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• 2006

Background: Current vascular prostheses are considered still inadequate for reconstruction of small-diameter vessels. To evaluate the potential use of xenograft vessels as small diameter arterial grafts, we implanted porcine vessels in goats. The grafts were treated with two different processes, freezing and acellularization, before implantation, and gross inspection as well as microscopic examination followed after a predetermined period. Material and Method: Bilateral porcine carotid arteries were harvested and immediately stored at $-70^{\circ}C$ within tissue preservation solution. One of them was designated as frozen xenograft vessel. The other one was put on acellularization process using NaCl-SDS solution and stored frozen until further use. Grafts were implanted in the place of carotid arteries of the same goat. The grafts have remained implanted for 1, 3, and 6 months in three animals, respectively. Periodic ultrasonographic examinations were performed during the observation period. After explantation, the grafts were analyzed grossly and histologically under light microscope. Result: All animals survived the experimental procedure without problems. Ultrasonographic examinations showed excellent patency of all the grafts during the observation period. Gross examination revealed nonthrombotic, patent lumens with smooth surfaces. Microscopic examinations of the explanted grafts showed cellular reconstruction at the 6-month stage in both grafts. Although more inflammatory responses were observed in the early phase of frozen xenografts, there was no evidence of significant rejection. Conclusion: These findings suggest that porcine xenograft vessels, regardless of pre-implantation processes of acelluarization or freezing, can be acceptably implanted in goats, although short duration of observation in a small number of animals may limit this study.

• The Korean Journal of Pesticide Science
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• v.13 no.3
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• pp.127-132
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• 2009

The purpose of this study is to examine changes in the pesticides residues in pepper powder by storage conditions. Analysis of pesticide residues was performed using multiresidue analytical methods and the number of pesticides detected from 60 pepper powders was 7 pesticides such as cypermethrin, fenvalerate, kresoxim-methyl, chlorpyrifos, chlorfenapyr azoxystrobin, tebuconazole. The concentrations of cypermethrin and tebuconazole were exceeded their maximum residue limits (MRLs). The degradation patterns of pesticides at $-5^{\circ}C$ were compared to those at $20^{\circ}C$ and $4^{\circ}C$. Without reference to storage temperature, degradation rates of azoxystrobin and fenvalerate were above 80%. Degradation rate of chlorpyrifos was 47% at storage $4^{\circ}C$, $20^{\circ}C$ and 34% at storage $-5^{\circ}C$. During each storage peroid, the degradation patterns were more lower at $-5^{\circ}C$ than at $20^{\circ}C$ and $4^{\circ}C$.

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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• v.29 no.7
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• pp.28-35
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• 2000

LNG 냉열 이용은 일본초저온(주)의 냉장보관 사업의 에너지절감 (65%)형 초저온 (-55$^{\circ}C$) 대형냉장고로 일본 뿐만 아니라 세계적으로 최초의 냉장업체이다. 회사 건설시는 입지선정, 경제성검토, 기술성, 공해상의 검토 등을 거쳐 완성된 업체로써 시설규모는 냉장 20,000 M/T, 1일 LNG 공급량 90M/T으로 현재 가동중이며 대부분의 동결 및 냉장 품목은 참치(마구로)가 83%를 차지하고 기타가 17% 였다. 냉장고의 가동율은 75%로 매우 높은 편이였다, LNG식 냉동장치의 가장 큰 장점은 일반적인 냉동방식 전력비의 1/3 밖에 소요되지 않는다는 것이다. L$N_2$의 내열이용 업체는 근기냉열(주)은 오사카의 천북에 있으며, L$N_2$를 자가 생산하여 면류 생산공장으로 직송하고 동결제조 하여 품질의 고급화 및 위생식품을 제조 판매하고 있다. 규모는 년간 2,000만식을 생산하고 주 생산제품은 조리면 및 냉동우동 등이다. L$N_2$의 소비가격은 전기식 냉동기에 비하여 5~9배의 고액이 소요되는 것이 결점이라고 할 수 있다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2011.10a
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• pp.738-741
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• 2011

창고관리 시스템은 RFID(Radio Frequency Identification) 기술을 이용하여 입 출고 관리에 대한 데이터를 자동으로 기록할 수 있을 뿐만 아니라 상품의 적재 위치를 정확히 보여주고, 적재된 상품의 현재 상태, 배치된 상품의 수량, 재고 상황 등의 정보를 실시간으로 확인 할 수 있게 해준다. 특히 냉동창고의 경우, 물품이 외부환경 요소에 민감하기 때문에 물품보관을 요청한 화주의 입장에서는 물품의 상태를 실시간으로 모니터링 하기를 원한다. 본 논문에서는 RFID 냉동창고를 이용하는 화주에게 본인의 물품상태를 실시간 원격으로 모니터링할 수 있도록 해 주는 스마트폰 기반의 애플리케이션의 개발내용에 대해 기술한다. 본 애플리케이션을 통해 화주는 물품의 입고요청, 출고요청을 실시간으로 보낼 수 있을 뿐만 아니라, 적재되어 있는 물품의 현재위치, 적재위치 이동경로, coldroom의 온도 등의 상태정보도 확인 할 수 있다. 냉동창고 업체는 RFID 냉동창고 관리시스템과 더불어 화주가 편리하게 이용할 수 있는 본 애플리케이션을 갖춤으로써 화주에 대한 서비스 향상에 기여하게 된다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2013.10a
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• pp.876-877
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• 2013

기존 식품건조 공정방법인 냉동보관, 자연건조에 의한 건조방식은 상품의 품질을 저하시켜 경쟁력이 낮고, 열풍건조 등 자동화된 건조공정의 경우에도 열에 의한 건조대상물의 탈색 및 변형 등의 특성변화로 인하여 고부가가치 상품의 제조하기 어려움의 한계로 인한 보관 및 상품성 저하 문제점 대두되고 있다. 따라서 이러한 문제점들을 해결하기 위하여 마이크로웨이브 건조기술 도입을 통한 건조방식 개선이 필요하다. 마이크로웨이브 건조기술은 표면 가열방식의 기존 건조방식에 비해 가열대상 물질의 표면 손상이 적고 전력효율이 우수하다는 특징으로 인하여 건조분야에서 점차 해당 건조기술이 확대되고 있다. 본 논문에서는 마이크로웨이브 건조에서 공정관리 요소들의 상태를 실시간으로 수집하고 이를 기반으로 숙련자의 작업공정 환경을 습득하여 데이터 베이스화하여 자동화된 작업공정을 제공하기 위한 모니터링 제어시스템을 제안하였다.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.6
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• pp.1453-1459
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• 2010

Recently, the aim of cold storage warehouse is not only to provide preservation of the quality of perishable foods but also to use state-of-the-art information systems to rapidly move products across the cold chain. An RFID (Radio Frequency Identification) is an automatic identification method that detects signals from mobile objects, and tracks and traces movement of the objects. RFID can be used to turn a cold storage warehouses into a real-time system. In this paper, we develop an cold storage management system to support the optimized business processes with RFID system. First of all, business processes in the cold storage warehouse are analyzed. Then design factors which should be considered for incorporating RFID are defined. Design values which set to the design factors can be extracted from using RFID deployment simulator. as a result, the design values make RFID to be efficiently integrated to existing business processes.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.2
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• pp.225-234
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• 2009

The aim of this study was to analyze moisture, crude protein, crude lipid, vitamin C and sugar changes in selected Korean chestnut cultivars such as Danteack, Deabo, Seokchu, Okkwang and Byunggo during storage at $4^{\circ}C$ and $-10^{\circ}C$ for 10 months. The moisture contents of selected Korean chestnut cultivars ranged from 49.9 to 57.4%. The moisture content of Seokchu was the highest. The contents of moisture in white kernel were higher than that in yellow kernel. The content of moisture showed decreasing tendency after 10 months of storage. The crude protein and crude lipid contents in whole kernel of selected Korean chestnut cultivars were $3.3{\sim}4.2%$ and $0.3{\sim}1.6%$, respectively. The crude protein content of Deabo was the highest. The crude protein in Danteack, Seokchu, Okkwang and Byunggo was increased during storage at $4^{\circ}C$ for 10 months, while that in whole kernel of Deabo was decreased and no changes in crude protein in yellow kernels of Deabo were observed. The crude protein in Okkwang was increased during storage at $-10^{\circ}C$ for 10 months. The cold storage was found to have higher composition change of crude protein than the freezing storage. The content of crude lipid in Daebo and Byunggo was decreased during storage at $4^{\circ}C$ and $-10^{\circ}C$. Yellow kernels of Deabo, Okkwang and Byunggo were found to have higher crude lipid content than white kernels. The vitamin C content also decreased during storage at $4^{\circ}C$ and $-10^{\circ}C$ and the decrease in vitamin C content was higher at $4^{\circ}C$ than $-10^{\circ}C$. Vitamin C was not detected after 3 months storage at $-10^{\circ}C$. The sugar content increased at the latter period storage at $4^{\circ}C$ and $-10^{\circ}C$. The sugar content of selected Korean chestnut cultivars ranged from $36.2{\sim}44.3%$ and Dantaek had the highest sugar content.

• The Korean Journal of Nuclear Medicine Technology
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• v.25 no.1
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• pp.29-33
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• 2021

Purpose Plasma renin activity (PRA) test is important for the diagnosis of primary aldosteronism. PRA is an easily deformed substance in vitro and affected by temperature changes. Laboratory of ASAN medical center has consistently found that there was a difference between the initial and re-experimental results. We compared and analyzed the differences in PRA test results according to the sample storage status. Materials and Methods The measurement of PRA was performed by using the radioimmunoassay. From August to September 2020, 43 PRA re-test samples were tested with different sample storage condition. The first group was re-examined by freezing the plasma-separated samples at -18℃, and the second group was re-examined with refrigerated EDTA sample. Also, additional tests were conducted on 13 PRA samples to verify the effect on thawing temperature differences in plasma-separated samples. The same samples were divided into two parts and stored frozen at -18℃, respectively, and thawing samples in room temperature and those in refrigerator were were conducted. Each result was compared and analyzed based on the initial experimental results. Results The results of re-examination after frozen storing plasma separation samples showed a lower correlation than the results of re-examination with EDTA plasma samples in refrigerator. When calculating the percentage based on the initial test results, the average percentage of each was 404.9% and 133.8%. The correlation coefficient was also R=0.8501 and R=0.9966, respectively, showing a higher correlation between plasma in the refrigerated sample EDTA tube. In comparison experiments with differences in thawing temperature, average percentage of the results of initial test and room temperature thawing was 94.3% and the average percentage of the results of refrigerated thawing was 88.0%. After again freezing the sample, the average percentage of the second room temperature thawing result is 107.5%, and the second refrigerated thawing group is 112.7%. Both groups showed an increase from first thawing. Conclusion A comparative analysis of retesting according to differences in sample storage methods in PRA tests showed a higher correlation between the results of retesting of the refrigerated EDTA plasma. And repeated freezing and melting of plasma separation samples, regardless of temperature during defrosting, has been shown to affect results. Therefore, retest of PRA should re-collect plasma from original EDTA plasma to increase reproducibility.

• Restorative Dentistry and Endodontics
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• v.33 no.4
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• pp.332-340
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• 2008

The purpose of this study was to evaluate the viability of periodontal ligament cell in rat teeth using slow cryopreservation method with magnetic field through MTT assay and TUNEL test. For each group, 12 teeth of 4 weeks old white female Sprague-Dawley rat were used for MTT assay, and 6 teeth in TUNEL test. The Maxillary left and right, first and second molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group1 (immediately extraction), group 2 (cold preservation at 4$^{\circ}C$ for 1 week), group 3 (rapid cryopreservation in liquid nitrogen), group 4 (slow cryopreservation with magnetic field of 1 G), and group 5 (slow cryopreservation). F medium was used as preservation medium and 10% DMSO as cryoprotectant. After preservation and thawing, the MTT assay and TUNEL test were processed. One way ANOVA and Scheffe method were performed at the 95% level of confidence. The value of optical density obtained after MTT analysis was divided by the value of eosin staining for tissue volume standardization. In both MTT assay and TUNEL test, it had showed no significant difference among group 3, 4, and 5. And group 3 had showed higher viability of periodontal ligament cell than group 2. From this study, slow cryopreservation method with magnetic field can be used as one of cryopreservation methods.

• Journal of Life Science
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• v.25 no.10
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• pp.1124-1131
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• 2015

Consumer interest in the stability of medicinal herb extracts during storage has increased. Although the advent of new technologies has improved preservation conditions, increasing the storage time, there are few studies on the preservation of herb extracts. The purpose of this study was to perform microscopic observations of Samul-tang decoctions under various preservation conditions. The storage temperature (a high temperature, room temperature, with or without light, refrigeration, or cryopreservation) and storage time (0, 15, 30, 90, and 180 days) were given to each condition Macroscopic morphology, pH, UV absorption, HPLC, and bacteriological studies were performed to determine microscopic changes in Samul-tang decoctions. The biological activity (tyrosinase inhibition) of the Samul-tang decoctions was also examined. There were no major changes in the indicated observation items when the extracts were stored in each condition. However, at higher storage temperatures and longer storage times, microscopic changes increased, although no bacteria were detected. Furthermore, the higher the storage temperature was and the longer the storage time was, the bigger the change was, despite of minor microscopic changes. Therefore, to maintain the stability of herbal extracts during storage, it is recommended to keep the Samul-tang decoction in the preservation condition of refrigeration and cryopreservation or without light rather than high temperature and room temperature as possible.