• Journal of Plant Biotechnology
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• v.42 no.2
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• pp.129-134
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• 2015

In order to compare salt tolerance among potato cultivars (Solanum tuberosum L.), in vitro plants of each cultivar were cultured on the Murashige and Skoog's medium containing different levels of NaCl (0, 75, 150, and 225 mM). The suitable level of NaCl for a comparison of the shoot and root growth between potato cultivars was 75mM. Shoot length and weight were better in 'Dejima', 'Superior', 'Jayoung', and 'Haryeong' than in other cultivars such as 'Goun' and 'Atlantic'. Normal root growth was observed in 'Seohong', 'Superior', and 'Haryeong', while 'Goun', 'Atlantic', 'Dejima', 'Jowon', and 'Chuback' showed no root. Proline contents in all the cultivars increased as affected by NaCl levels, then the increase amount was fewer in the cultivars showed a better growth in both shoot and root than in others showed a poor growth. As a result of this study, 'Superior' and 'Haryeong' were determined as salt tolerant cultivars and therefore using these cultivars it would be possible to potato production at salt arable land such as reclaimed tidal.

• Journal of Plant Biotechnology
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• v.41 no.2
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• pp.81-88
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• 2014

Salt cress (Thellungiella halophila or Thellungiella parvula), species closely related to Arabidopsis thaliana, represents an extremophile adapted to harsh saline environments. To isolate salt-tolerance genes from this species, we constructed a cDNA library from roots and leaves of salt cress plants treated with 200 mM NaCl. This cDNA library was subsequently shuttled into the destination binary vector [driven by the cauliflower mosaic virus (CaMV) 35S promoter] designed for plant transformation and expression via recombination- assisted cloning. In total, 305,400 pools of transgenic BASTA-resistant lines were generated in Arabidopsis using either T. halophila or T. parvula cDNA libraries. These were used for functional screening of genes involved in salt tolerance. Among these pools, 168,500 pools were used for primary screening to date from which 7,157 lines showed apparent salt tolerant-phenotypes in the initial screen. A secondary screen has now identified 165 salt tolerant transgenic lines using 1,551 (10.6%) lines that emerged in the first screen. The prevalent phenotype in these lines includes accelerated seed germination often accompanied by faster root growth compared to WT Arabidopsis under salt stress condition. In addition, other lines showed non-typical development of stems and flowers compared to WT Arabidopsis. Based on the close relationship of the tolerant species to the target species we suggest this approach as an appropriate method for the large-scale identification of salt tolerance genes from salt cress.

• Korean Journal of Plant Tissue Culture
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• v.25 no.2
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• pp.103-107
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• 1998

Tobacco proembryos were irradiated with 100 Gy of heavy-ion beams($^{14}\textrm{N}$, $^{20}\textrm{Ne}$: 135 Mev/u) after 24 to 96 hours of pollination as a mutagen and screened $\textrm{M}_{1}$ generation for morphological mutants and salt-tolerant plants. Morphological and physiological characteristics of the salt-tolerant plants derived from the irradiated proembryo are discussed in this report. Mutants irradiated proembryos with the beams after pollination produced various kinds of morphological variation. A total of 17 salt-tolerant plants were selected from tobacco cultivar (BY-4) by treatment with $^{14}\textrm{N}$ beam. Shapes of filament and pollen grain of most salt-tolerant plants were abnormal compared with non-irradiated wild type, and seeds weight and fertility obviously decreased. The germination rates of the several $\textrm{M}_{2}$ lines on the saline and the mannitol condition were higher than that of wild type.

• Journal of Plant Biology
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• v.13 no.4
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• pp.23-31
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• 1970

The rice variety, Kwanok, was reared in the water and land beds and transplanted to the reclaimed soil area, having an average salt concentration of 0.39%. Two levels of the moderate and late season cultures with 4 treatments were used. The K and Si contents of the stem part of land bed seedlings were somewhat smaller, but total carbohydrate remarkably larger, the C/N ratio was accordingly greater than water bed seedlings. The rooting ability of land bed seedlings was vigorous markedly in culture solutions, to which added various concentrations of NaCl, The rooting ability of each seedling water not much declined in theculturing solution of up to 9.4mmhos/cm, (0.6%) of salt concentration, but it was drastically declined in the salt concentration over that. It seemed that the critical salt concentration for the rooting rice plant. The land bed seedlings in each salty condition markedly decreased compared with the water bed seeldings in transpiration rate and it showed a stronger drought resistance and contained a large amount of chlorophyll at transplanting stage, and also showed higher stability of chlorophyll at rooting stage in the salt treatment. The number of panicles, panicle weight, number of grains per panicle and ratio of matured grains of the rice plant grown by the land bed seedlings were much greater and 1,000 grain weight was less than from water bed seedlings. The cultural practices with the land bed seedlings increased the rough rice yields by 15% and 11%, respectively, compared with the yields of the moderate and late season cultures by water bed seedlings.

• Korean Journal of Plant Tissue Culture
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• v.28 no.1
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• pp.47-52
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• 2001

Bet A and Bet B genes related to salt resistance were introduced into Lycium chinense through high efficiencies of plant regeneration. The explants were precultured on the shooting medium which is consisted of MS medium added 1mg/L kinetin and 0.05mg/L IBA for 2 days. After pre-culture, they were immersed in LB media containing Agrobacteria tumefaciens harboring Bet genes, and cultured on the same medium. Putative transformants could be selected after cocultivation of the explants with Agrobacteria on the shooting medium supplemented with 30mg/L kanamycin. The presence of both Bet A and Bet B genes from the transgenic plants were confirmed by PCR amplification with the gene specific primers and subsequent PCR-Southern blot with labeled Bet genes probe. The expression of Bet A and Bet B genes in the transgenic plants were observed by RT-PCR method.

• Journal of Plant Biology
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• v.13 no.4
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• pp.13-22
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• 1970

The rice variety, Kwanok, was reared in the water, land and salt seed beds and transplanted to the reclaimed soil area having an average of 0.48% salt content (0.67% at the end of April). The plant height of land bel seedlings at transplanting stage was short but the dry-weight/plant-height ratio was large and the rooting ability was vigorous remarkably after transplantation in the salty area. The central cylinder, vessels, sclerenchyma, endodermis and other mechanical tissues of the root of land bed seedlings were well developed while the size of cortical cell layers were small. The cytoplasm of the cortical parenchyma at the root tips seemed to be most abundant in the land bed seedlings. The formation of the aerial cavity in the cortex of primary root was rapid and it seemed that the developmental mechanism of the aerial cavity in the rice plant roots was related to the development of the lateral roots.

• Journal of Plant Biology
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• v.13 no.1
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• pp.1-12
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• 1970

The Nongkwang variety was sown directly in the field by the three methods of dibbling, drill seeding and broad-casting in non-, low- and high-salty areas, and compared with the growth of transplanted rice in each areas. The yield of rough rice was increased remarkably by direct sowing when compared with that produced by transplanting inboth salty areas, but in non-salty area, the former was decreased significantly. There was no significant difference in the yields of rough rice resulting from three seedling methods of direct sowing in each area. The direct sowing method was predominated in number of panicle, weight of panicle, ratio of matured grains, milling recovery percentage, and straw weight in both salty areas, and dicreased in panicle weight and ratio of matured grain in nonsalty area.

• Journal of Plant Biology
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• v.14 no.3
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• pp.60-65
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• 1971

In order to observe the factors affecting light utilization efficiency in the canopy of a rice crop grown in reclaimed saline areas in Korea, 22 varieties of rice were cultured at the average salt concentration of 6.05mmhos/cm $25^{\circ}C$, (0.39%) in saline soils and non-saline silty loam soils. The inhibition of tillering ability by salt damage at the maximum tillering stage was greater than to growth in height. A significant direct correlation was observed between both ratios and salt concentration with each variety. In the salty areas the length of the flag leaf of the maturing stage showed a positive correlation with the production of rough rice while other living leaf showed a negative correlation. LAI of maturing stage was less in the salty area than in the non-salty area, while the former showed higher ratio in net assimilation and translocation to head with greater RGR before and after the heading stage.

• Journal of Plant Biology
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• v.14 no.1
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• pp.25-31
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• 1971

In order to observe the absorption pattern of mineral elements and the nutritional components of brown rice grown in reclaimed saline areas in Korea, the four commercial varieties of rice were cultured at the average salt concentration of 6.05 mmhos/cm, $25^{\circ}C$, (0.3%) in saline soils and non-saline silty loam soils. In the saline areas the absorptin of Na and Si was relatively high while the absorption of P, K and Ca was relatively low. N was absorbed actively before the emergence of the rice grains but showed relative decrease thereafter. There was no difference in Mg content. Though brown rice in the saline areas contained sugar abundantly with small contents of protein, fat and crude fiber. There was little difference in total carbollydrate and ash contonts.

• Journal of Applied Biological Chemistry
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• v.60 no.2
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• pp.125-131
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• 2017

Nine strains of high concentrations of salt-tolerant bacteria were isolated from the rhizosphere and rhizoplane of the halophyte plant Suaeda japonica grown in Gochang Buan tidal flat. The isolated bacteria were classified as genera Vibrio (strains JRS-1, -2, -3, -4, and -5, and JRL-1 and -4) and Bacillus (strains JRL-2 and -3) based on the 16S rRNA gene sequence similarity. The optical growth condition for salt concentration was examined on the selected, representative strains. Strain JRS-1 with the closest relative of Vibrio neocaledonicus showed the highest growth rate at the total salt concentration of 6% among the incubation conditions of 3-8% salt concentrations. Strain JRL-2 with the closest relative of Bacillus thuringiensis showed the tendency that growth rate increased with increasing salt concentrations and the maximum growth rate at 7% of the total salt concentration. The isolated bacteria showed salt-resistances to higher salt concentrations than their habitat soils with 3%. In addition, we identified evidences of potentially plant interaction-relevant enzymatic activities, from utilization of some substrates rich in plants, such as triglyceride, ${\rho}$-nitrophenyl-${\alpha}$,$\text\tiny{D}$-glucoside, and ${\rho}$-nitrophenyl-${\beta}$,$\text\tiny{D}$-glucoside.