• Title/Summary/Keyword: 내분비

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Safty of Alternatives for Endocirne Disrupting Substances (내분비계장애물질 대체소재의 안전성)

  • Park, Chan Jin;Kim, Woong;Gye, Myung Chan
    • Korean Journal of Environmental Biology
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    • v.33 no.4
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    • pp.361-374
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    • 2015
  • Endocirne disruptors (EDs) can cause fertility decrease, developmental disorder, and even cancer in animals. Until 90's, EDs were used in various synthetic products including paints, coatings, detergents, plastics, and plasticizers. Currently, in several countries, the production, trade and use of EDs or EDs-suspected chemicals have been regulated while activity to screen the alternatives for EDs including bisphenol-A, phthalate and nonylphenol is active. Although various toxicity test method was developed and applied for screening of alternatives, however, the safety of alternatives has been not fully demonstrated. Some alternatives have high structural similarity with existing EDs, raising the possible risk of endocrine disruption by alternatives. In an effort to develop the safe alternatives, we reviewed the effects of EDs such as bisphenol-A, phthalates, nonylphenol and their substituents. In addition, in-silico analysis for endocrine disrupting activities of some alternatives was presented.

An immunohistochemical study of the gastro-entero endocrine cells in the snakehead, Channa(ophicephalus) argus (가물치 위장관에 있어서 내분비세포의 면역조직화학적 연구)

  • Lee, Hyeung-sik;Lee, Jae-hyun
    • Korean Journal of Veterinary Research
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    • v.36 no.2
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    • pp.297-303
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    • 1996
  • The regional distribution and relative frequency of endocrine cells in the GIT of the snakehead, Channa(Oph icephalus) argus were studied immunohistochemically. Five kinds of endocrine cells and one kind of nerve cell were identified in this study. A few numbers of secretin-immunoreactive cells were restricted to the cardia and fundus of the stomach. Moti-lin- and GRP-immunoreactive cells were rare in the epithelium of the distal and proximal intestines. PYY-immunoreactive cells were found in a few number in the pyloric caeca and were rare in the distal intestine. Substance P-immunoreactive cells were distributed relatively numerous from the fundus to the distal intetsine. Also, their nerve cells were detected in occurrence in the lamina propria in the distal intestine. No neurotensin-, met-Enk- and GIP-immunoreactive cells were found in the GIT of the snakehead.

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Ultrastructural and Histochemical Study on the Epithelia of Digestive Tract of a Korean Slug, Incilaria fruhstorferi (한국산 산민달팽이 ( Incilaria fruhstorferi ) 소화관 상피조직의 미세구조 및 조직화학적 연구)

  • 이정찬;장남섭;한종민
    • The Korean Journal of Malacology
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    • v.13 no.2
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    • pp.143-160
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    • 1997
  • 한국산 산민달팽이 Incilariafruhstorferi의 소화관을 부위별로 관찰하고, 소화관 상피조직을 구성하는 세포의 종류와 분포수 그리고 분비되는 과립 등을 미세구조적, 조직화학적으로 관찰하고 그 결과를 보고하고자 한다. 산민달팽이의 소화관은 식도, 위, 장, 직장 등으로 구성되어있으며, 식도는 다시 전식도, 소낭, 후식도로 나누어지고, 장은 전장과 중장, 후장 등으로 구분되었다. 소화관을 부위별롸 관찰한 결과 섬모원주상피세포 2종, 녹색과립세포 3종, 청색과립세포, 점액세포, 투명세포, 망상형세포 그리고 괴사형세포 등 모두 10종류가 확인되었다. 섬모원주세포는 A형과 B형 등 두 종으로 나뉘며, A형은 세포의 상단 자유면에 섬모와 미세융모가 밀생된데 비해, B형은 전자밀도가 높아서 검게 보이고, 장과 직장에서만 관찰되는 특징을 보였다. 섬모의 구조는 9 x 2 + 2 axoneme이었다. 녹색과립세포는 미세구조에 의해 A형, B형 그리고 C형등 3종으로 나누어지며, 소낭과 후식도, 위, 직장에서 주로 관찰되었다. A형과 B형은 Sudan black에 양성인 지방과립(1.36 x 1.6$\mu\textrm{m}$)만을 포함하고 있는데비해 C형은 글리코겐 과립도 포함하고 있었다. 청색과립세포는 10종류의 세포 중 가장 키가 크고 (35$\mu\textrm{m}$), Millon반응에 양성을 보이는 둥근과립(직경, 1.3$\mu\textrm{m}$)을 포함하고 있는데 과립들은 단백질성으로 확인되었다. 이들은 중장에서만 관찰되었다. 장과 직장에서 주로 많이 관찰되는 점액세포는 세포질 속에 전자밀도가 낮은 투명한 과립과 전자밀도가 높아서 검게 보이는 둥근과립(크기, 1.33 x 0.89$\mu\textrm{m}$)들을 포함하고 있었는데, 이들은 미성숙 시기에는 투명과립(직경, 2.66$\mu\textrm{m}$)으로 관찰되었다. PAS-alcian blue(pH 2.5)반응에서 투명과립은 alcianophillia로 나타난 반면, 검은 과립은 PAS에 양성반을을 보이며, 각각 산성점액과 중성점액과립으로 확인되었다. 투명세포는 광학현미경 관찰에서 A형과 B형으로 구분되었으나, A형은 전자현미경 관찰에서 신경내분비세포로 확인된 반며, B형은 지방과립을 소지한 지방저장세포로 각각 확인되었다. 신경내분비세포가 소지한 과립의 크기는 0.16$\mu\textrm{m}$ 정도였다. 망상형세포는 주로 위에서 관찰되는 형태가 불규칙한 작은 세포로서 세포질에 비해 큰 핵을 소지하고 있었다. 소량의 세포질 돌기에는 사립체와 과립성소포체를 포함하고 있다. 괴사형세포는 후장과 직장에서 주로 많이 관찰되고, 점액세포의 점액과립이 분비된 후, 붕괴되는 과정에서 형성된 것으로 확인되었다.

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An immunohistochemical study of the endocrine cells in gastrointestinal tract of the Korean native goat (한국 재래산양의 위장관에 출현하는 내분비세포의 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.30 no.3
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    • pp.261-270
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    • 1990
  • The gastrointestinal endocrine cells of the Korean native goat were studied immunohistochemically, and 5-HT-, somatostatin-, Gas/CCK-, glucagon-, chromogranin- and PP- immunoreactive cells were revealed. The characteristic findings of the regional distribution and relative frequency of these immunoreactive cells in the gastrointestinal tract of the Korean native goat were as follows. 5-HT-immunoreactive cells were more numerous in the small and large intestine than in the abdomen stomach. Somatostatin-immunoreactive cell were more numerous in the abdomen stomach than in the small and large intestine. Gas/CCK-immunoreactive cells were concentrated very numerously in the pyloric region with a few in the other regions. Moderate numbers of glucagon-immunoreactive cells were found in the small and large intestine, but a few of them were found in the abdomen stomach. Very numerous chromogranin-immunoreactive cells were detected throughout the gastrointestinal tract. PP-immunoreactive cells were observed moderate numbers in the large intestine with few in the ileum. No insulin-immunoreactive cell was found in the gastrointestinal tract.

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An Ultrastructural Study on Endocrine Cells in the Pyloric Region of the Korean Hedgehog(Erinaceus koreanus) (한국산 고슴도치 유문부에 있어서 내분비세포의 전자현미경적 연구)

  • Lee, Jae-Hyun
    • Applied Microscopy
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    • v.17 no.1
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    • pp.177-184
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    • 1987
  • Endocrine cells in the pyloric region of the hedgehog were studied ultrastructurally. 4 types of endocrine cells classified as gastric-type EC, ELC, $D_1$, and G cells were observed in this region. The gastric-type EC cells contained pleomorphic granules with high electron density and highly dense bodies in a dense matrix. ECL cells were characterized by the presence of round or oval granules with high electron density. Some granules of ECL cells showed a small amount of content or empty. $D_1$ cells contained round and small granules with low electron density. They occasionally showed a narrow halo between the limiting membrane and the dense materials. G cells were characterized by the presence of round or oval granules with low electron density. Some granules of these cells showed a narrow halo between the limiting membrane and the core.

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An immunohistochemical study of the endocrine cells on the gastro-entero-pancreatic system of the African clawed toad, Xenopus laevis (아프리카발톱두꺼비(Xenopus laevis)의 위장췌내분비세포의 면역조직화학적 연구)

  • Lee, Hyeung-sik;Lee, Jae-hyun
    • Korean Journal of Veterinary Research
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    • v.32 no.4
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    • pp.523-529
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    • 1992
  • The GEP endocrine cells of the African clawed toad, Xenopus laevis, were studied immunohistochemically. Five kinds of the endocrine cells were identified in this study A moderated number of 5-HT-immunoreactive cells were detected throughout the gastro intestinal tract, being almost uniform frequency. Gas/CCK -immunoreactive cells were restricted to the basal portion of the pyloric gland and among the duodenal mucosa. A rare glucagon-immunoreactive cells were weakly reacted in the fundic region of the stomach and observed in the exocrine portions of the pancreas. Somatostatine-immunoreactive cells were distributed throughout the gastrointestinal tract with except for the rectum, and not only the periphery of the islets but also the exocrine portions in the pancreas. No CGs- and insulin-immunoreactive cells were found in the gastrointestinal tract, whereas in the pancreas, the later was seen in the central region of the islets and the exocrine portions.

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The Ultrastructure of the Gastro-endocrine Cells in the Gastric Mucosa of the Frog, Rana rugosa (옴개구리 Rana rugosa 위점막의 위장관 내분비세포에 관한 전자현미경적 연구)

  • 이형식;이재현
    • The Korean Journal of Zoology
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    • v.33 no.1
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    • pp.22-27
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    • 1990
  • The gastro-endocrine cells were examined in the fundic region of stomach of the frog, Rana rugosa, by transmission electron microscope. In the present paper, at least three kinds of cell type distributed in this region were idendfted according to their morphological charactedstics based on the size, shape and electron density of the secretory granules. Type I cells were characterized by the presence of round or oval granules (300-5OOnm in diameter) with high electron density. The granules showed a wide lucent between the contents and the limiting membrane. This cell was reminiscent of the ECL cell in the human alimentary mucosa. Type II cells were characterized by the presence of spherical or oval granules (11O-230nm in diameter) with low or high electron density. The granules showed a clear halo between the homogenous contents and the limiting membrane. This cell was reminiscent of the G cell in the human alimentary mucosa. Type Ill cells were characterized by the presence of elongated oval or pheomorphic granules (50-2OOnm in diameter) with low or moderate electron density and abundant microfilament bundles in the cytoplasm. The granules contained a very thin halo.

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An immunohistochemical study of the pancreatic endocrine cells in the cat-shark, Scyliorhinus torazame (두툽상어 췌장에 출현하는 내분비세포의 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Nam-soo;Lee, Hyeung-sik;Kim, Jong-beom
    • Korean Journal of Veterinary Research
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    • v.31 no.1
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    • pp.27-32
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    • 1991
  • The pancreatic endocrine cells of the cat-shark, S. torazame, were studied using immunohistochemical method. Five kinds of endocrine cells (glucagon-, somatostatin-, insulin-, 5-HT-and BPP-immunoreactive cells) identified in this study. The chracteristic findings of the distributions of five immunoreactive cells were as follows. Glucagon-immunoreactive cells were detected as clustering group in the epithelia of the interlobular duct and singly the pancreatic acini, respectively. Insulin -immunoreactive cells were moderately observed in the epithelia of the interlobular duct or in the periphery of the islet. Somatostatin-immunoreactive cells were distributed in single or mass groups in the epithelia of the interlobular duct and the exocrine gland of the pancreas. A very few 5-HTimmunoreactive cells were seen in the periphery of the islet and the acini of the pancreas-BPP-immunoreactive cell was singly located in the periphery of the pancreatic islet, but GAS/CCK-and Chromogranin-immunoreactive cells were not found in this study.

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An immunohistochemical study of the endocrine cells in the stomach of the Korean hedgehog(Erinaceus korean us) (고슴도치 위점막의 내분비세포에 관한 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik;Lee, Nam-soo;Kim, Jong-beom
    • Korean Journal of Veterinary Research
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    • v.31 no.1
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    • pp.19-26
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    • 1991
  • The gastric endocrine cells of the Korean hedgehog, Erinaceus korean us were studied immunohistochemically. Seven kinds of endocrine cells-, gastrin-, somatostatin-, 5-HT-, glucagon-, BPP-, motiIin-and GIP-immunoreactive cells- were identified in this study. The chracteristic findings of the regional distribution and relative frequency of them were examined. Gastrin-immunoreactive cells were very numerously detected only in the pyloric region. Somatostatin-immunoreactive cells were more numerous in the pyloric region than in the cardiac and fundic regions. 5-HT-immunoreactive cells were more numerous in the cardiac and pyloric regions than in the tundic one. Glucagon-immunoreactive cells were found few or rarely in the fundic and pyloric regions. BPP-imunoreactive cells were numerously distributed in the pyloric region, moderately in the fundic region and few in the cardiac region. Motilin-immunoreactive cells were found rarely or few in the fundic and pyloric regions. GIP-immnuoreactive Cells were detected onIy in the pyloric region.

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Immunohistochemical study of the gastrointestinal endocrine cells in the Mongolian Gerbils, Meriones unguiculatus (몽골리안 저빌(Meriones unguiculatus)의 위장관 내분비 세포에 대한 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik;Ku, Sae-kwang;Park, Ki-dae;Kim, Kil-soo
    • Korean Journal of Veterinary Research
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    • v.40 no.4
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    • pp.653-660
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    • 2000
  • In order to study the regional distribution and relative frequency of the immunoreactive endocrine cells in the gastrointestinal tract of the Mongolian gerbil, Meriones unguiculatus, the gastrointestinal tract was divided into 9 portions (cardia, fundus, pylorus, duodenum, jejunum, ileum, cecum, colon and rectum) and immunostained by immunohistochemical (PAP) method using 8 types of specific antisera against cholecystokinin (CCK)-8, gastrin, secretin, pancreatic polypeptide(PP), somatostatin, serotonin, glucagon and insulin. CCK-8-, gastrin-, somatostatin- and serotonin-immunoreactive cells were demonstrated in this study. These immunoreactive cells were found in the gastric gland regions of the pylorus or between parietal and chief cells of the fundus with round to spherical shaped, and in the interepithelial regions of the intestinal tract with spherical to spindle shaped except for jejunum where some of immunoreactive cells were also observed in the intestinal glands with round to spherical shaped. CCK-8-immunoreactive cells were restricted to the pylorus and duodenum with numerous and a few frequency, respectively. Gastrin-immunoreactive cells were restricted to the pylorus with numerous frequency. Similar to those of gastrin-immunoreactive cells, somatostatin-immunoreactive cells were restricted to pylorus with moderate frequency. Serotonin-immunoreactive cells were detected throughout whole gastrointestinal tract except for cardia and cecum with moderate or numerous frequency. However, no secretin-, PP-, glucagon- and insulin-immunoreactive cells were observed in this study. From these results, the appearance type, regional distribution and relative frequency of immunoreactive endocrine cells in the gastrointestinal tract of the Mongolian gerbils were somewhat lowered or restricted compared to those of other mammals and these differences were might be caused by feeding habits and species specification.

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