• Korean journal of applied entomology
• /
• v.57 no.4
• /
• pp.329-337
• /
• 2018

To increase the mating rate of Bombus ignitus used as insect pollinator, we investigated the sexual maturity time of B. ignitus. In investigating ovary development such as the number of eggs per ovariole and spermatheca size, the time of sexual maturity of queen was 10 days after eclosion. In case of male, the number of sperm was 246 thousand at immediately after eclosion, and was highest as 480 thousand at 9 days, and tended to show a dramatic decline at 35 days (87 thousand). The more mating time, the less the number of sperm. In consideration of number of sperms, the time of sexual maturity of male was 3-15 days after eclosion. In the sexual maturity time of queen in mating, the queen was not mated at immediately after eclosion, and showed a decrease in 20 days. In terms of the rate of mating and oviposition, the favorable time for mating of queen was 9-20 days. On the other hand, the male showed 3.3% of mating at immediately after ecolosion, showed the highest of 43.3% at 6 days after eclosion, and tended to decrease in 25 days. The sexual maturity time for mating of male was 6-20 days. In summary, our results indicate that sexual maturity time of B. ignitus in reproductive organs and mating is most favorable in 9-12 days after eclosion for queen and 6-9 days for male.

• Korean journal of applied entomology
• /
• v.59 no.1
• /
• pp.25-35
• /
• 2020

The striped fruit fly, Zeugodacus scutellata, is endemic in Korea, but it has been regarded as one of the serious quarantine pests throughout the world. Sterile insect release technique (SIT) has been used to eradicate quarantine fruit flies. This study developed a technique to generate sterile males and applied SIT to control Z. scutellata. First of all, the reproductive systems of Z. scutellata adults were examined with fluorescent microscope. Polytrophic ovaries comprises of around 100 follicles with developing oocytes. Each follicle contains an oocyte with several nurse cells and are surrounded with follicular epithelium. Oocyte development began at 10 days after adult emergence (DAE) and formed chorionated oocytes after 20 DAE. On the other hand, male testes were well developed just after adult emergence. The vas deferens was filled with motile sperms. To generate sterile males, different doses (0~1,000 Gy) doses of electron beam were irradiated to 3~5 days old pupae of Z. scutellata. When male pupae were irradiated with electron beam at 200 Gy, they developed and mated with females without any significant difference compared to untreated males. Although the untreated females mated with the 200 Gy-irradiated males laid eggs, no eggs did not hatch. The 200 Gy-irradiated males were then applied to untreated male and female flies in a density ratio of 1:9 (untreated males : treated males). The laid eggs suffered significant infertility. These results suggest that electron beam-irradiated pupae at 200 Gy resulted in male sterility and the resulting males would be applied to SIT.

• Proceedings of the KSAR Conference
• /
• 2001.03a
• /
• pp.75-75
• /
• 2001

유리화 동결법은 동결중 ice crystal의 형성이 이루어지지 않으므로 난자의 동결보존을 위한 유용한 동결방법이다. 이전의 연구에서 마우스의 난자를 ethylene glycol과 electron microscope grid를 이용한 유리화 동결법으로 동결 융해한 결과 기존의 slow freezing 방법에서보다 높은 생존율과 배발달율이 나타남을 관찰하였다. 그러나 동물과 인간 난자를 이용한 연구를 통하여 난자의 경우 동결 융해후 염색체에 부착되어 있는 미세소관인 방추사가 온도의 변화에 매우 민감하여 염색체 이상성이 증가되는 것이 보고되었다. 이에 본 연구에서는 성숙난자를 유리화동결법에 의해 동결 융해후 난자의 염색체와 방추사의 이상성이 증가되는지 알아보고자 본 실험을 수행하였다. ICR mouse의 성숙란을 채취하여 연구목적에 따라 fresh한 대조군과 동결음해 시킨 실험군으로 분류하였다. 동결방법은 난자를 1.5 M ethylene glycol (EG)에 2분 30초간 노출 시킨후 5.5 M EG와 1M sucrose가 첨가된 동결액에 20초간 노출시킨 후 Grid에 난자를 부착시킨 후 직접 액체질소에 침지하여 동결하였다. 동결후 난자는 5단계로 융해를 실시한 후 생존된 난자는 tubulin 항체를 이용한 immunostaining 방법으로 방추사의 이상성을 관찰하였고, 염색체는 난자를 고정 후 10% Giemza로 염색 후 염색체의 수적인 이상성을 관찰하였다. 염색체 분석결과 염색체 이상 빈도는 대조군의 경우 19.6%, 동결융해군은 32.8%로 관찰되었다. 또 방추사의 이상빈도는 대조군의 경우 20.2%, 동결 융해군은 32.3%로 관찰되어 동결 융해후의 난자에서 염색체와 방추사의 이상 빈도가 증가됨이 관찰되었다.찰되었다. 배아이식후 대조군과 실험군에서 각각 2 마리가 임신이되어 정상적인 산자를 분만하였다. 따라서 항동해제에 taxol의 첨가는 동결 융해후의 난자의 배발달율을 증진시킬 수 있었다..8%로 나타나 난할율 및 배반포 발생율에 있어서 융합조건에 따라 큰 차이는 없었으나 1.9㎸/cm, 30$\mu\textrm{s}$ 2회의 조건이 다른 조건들에 비하여 유의적으로 낮았다. 따라서, 체세포와 수핵란 세포질간의 융합율과 배반포 발생에 미치는 영향은 전압보다는 시간에 더 크게 받음을 알 수 있었으며, 이와 같은 결과에서 융합시 시간을 오래 주는 것보다 전압을 높이는 것이 수핵난자의 세포질에 상해를 줄이고 이후 배반포 발생에 유리할 것으로 사료되었다.면에서도 더욱 더 활발할 것으로 기대된다. 배란후 72시간째에 초음파진단기를 이용하여 난소의 난포발달을 조사한 결과 , 대조구와 bFF처리구에 비해 AI처리구에서 발달난포가 유의적으로 많은 것을 확인하였다. 이상과 같은 결과로, Anti-inhibin serum은 한우 자체에서 분비하는 Inhibin을 특이하게 억제하여 Inhibin에 의해 억제되는 FSH분비가 촉진됨으로써 난포발달과 estrogen의 농도가 촉진되는 것으로 사료되어 anti-inhibin serum이 한우의 과배란유기 효과가 있는 것으로 사료된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성 물질로 증착시키지 않고, Silver Paint 에 시편을 접착하는 방법으로도 만족한 시험 결과를 얻을 수 있었다.째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간을 연장시키는 경향을 보이며

• Applied Microscopy
• /
• v.30 no.1
• /
• pp.45-59
• /
• 2000

Cis-platin is a widely used anticancer drug against certain solid tumors such as malignant ovarian tumor, malignant carcinoma of head and neck, bladder cancer and cervical cancer of uterus, and its major mechanism of action is inhibition of DNA synthesis of the tumor cell. To investigate the inhibitory effects of cis-platin on the ciliogensis of the ciliated cells in the mucosa of oviduct, the author pursued the alterations of $\alpha-tubulin$, which is the main constituent of the microtubles in cilia, after cis-platin treatment. To eliminate the possible variations due to ovarian cycle, female Spargue-Dawley rats ($150\sim200gm$ in B.W.) were pretreated with estradiol benzoate (20 mg/kg, once a day, for 4 consecutive days). Animals were administrated with cis-platin (6 mg/kg, i.p.) and sacrificed at 1day, 3days, 5days and 7days after treatment, respectively. Immunohistochemistry for $\alpha-tubulin$ using mouse anti-rat $\alpha-tubulin$ monoclonal antibody as primary antibody was done. Immunogold electronmicroscopy for intracellular distributions of $\alpha-tubulin$ was also performed with same primary antibody and Goat anti- mouse IgM which is preconjugated with gold particles of 15 nm as secondary antibody. The results obtained were as follows; 1. Strong immunoreactivity of $\alpha-tubulin$ was observed in ciliated cells of oviducts at 1, 3 and 5 days after estradiol pretreatment. 2. Weak immunoreactivity of $\alpha-tubulin$ was observed in ciliated cells of oviducts at 1 and 3 days after cis-platin treatment but it was recovered to strong immunoreactivity in 5 days 3. In immunogold electronmicroscopy, density of gold particles for $\alpha-tubulin$ reactions was decreased in apical cytoplasm, but few changes were observed in basal body or cilia at 1 and 3 days after cis-platin treatment. From these above results, it is indicated that synthesis of $\alpha-tubulin$ in ciliated cells of rat oviduct is inhibited by cis-platin treatment.