• Korean Journal of Plant Taxonomy
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• v.39 no.3
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• pp.135-142
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• 2009

Phylogeny of the family Ophioglossaceae and a phylogenetic position of Mankyua were estimated through analyses of chloroplast rbcL gene sequences and spore morphology. Sequence analysis of the rbcL gene clearly indicated that there are two major lineages in the family Ophioglossaceae: Botrychioid lineage and Ophioglossoid lineage. The Botrichioid lineage is composed of three distinct clades: Botrychium, Helminthostachys and Mankyua, where Helminthostachys and Mankyua were placed as sister groups to the Botrychium. Within the genus Botrychium, subgenera Septridium and Botrychium were monophyletic, while taxa of subgen. Botrypus branched as sister of the two, successively, thus making a non-monophyletic group. Ophioglossum formed the Ophioglossoied lineage, where the subgen. Ophioglossum is monophyletic, while subgen. Cheiroglossa and Ophoderma formed a sister relationship with subgen. Ophioglossum. In terms of external morphology and spores, Mankyua is most similar to Helminthostachys, however, patristic distance in the cladogram and trophophore characteristics of the two genera are distinct. Therefore, Mankyua is a well defined genus within the family in terms of morphology as well as molecular phylogeny which places it in basal position of the Botrychioid lineage on the gene tree.

• Journal of Plant Biology
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• v.37 no.1
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• pp.43-51
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• 1994

Spore morphology of 14 species of Ophioglossaceae is examined, and descriptions and key based on spore morphology are provided. In addition, spores of one species of Angiopteris and three species of Osmunda are examined for comparision with those of Ophioglossaceae. Spore shape at proximal face is sub triangular or triquete in Botrychium and circular in Ophiog~ lossum, Angiopteris and Osmunda. Based on the wall sculpturing patterns, three groups can be recognized within Botrychium, and the differences of wall sculpturing among these groups are smaller as compared to those among Botrychium, Ophioglossum, Osmunda and Angiopteris. This result supports the previous infrafamilial system of Clausen, which was mainly based on the characteristics of vernation, habit, fertile and sterile segments of the leaf, and gametophyte. Spores of nine species of Ophioglossum, which are circular in proximal face and fossulate or foveolate in wall sculpturing, are quite uniform as compared to those of Botrychium. Therefore they should be kept under a single genus rather than split into four genera suggested by Nishida (1952).(1952).