• 한국지능시스템학회논문지
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• 제26권5호
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• pp.343-350
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• 2016

Remaining lifetime prediction of the underground gas pipeline plays a key role in maintenance planning and public safety. One of main causes in the pipeline failure is metal corrosion. This paper deals with estimating the pipeline reliability in the presence of corrosion defects. Because a pipeline has uncertainty and variability in its operation, probabilistic approximation approaches such as first order second moment (FOSM), first order reliability method (FORM), second order reliability method (SORM), and Monte Carlo simulation (MCS) are widely employed for pipeline reliability predictions. This paper presents a fuzzy inference based reliability method (FIRM). Compared with existing methods, a distinction of our method is to incorporate a fuzzy inference into quantifying degrees of variability in corrosion defects. As metal corrosion depends on the service environment, this feature makes it easier to obtain practical predictions. Numerical experiments are conducted by using a field dataset. The result indicates that the proposed method works well and, in particular, it provides more advisory estimations of the remaining lifetime of the gas pipeline.

• 한국연초학회지
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• 제31권1호
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• pp.45-50
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• 2009

In this study, a simple gradient RRLC method for rapid determination of carbonyl compounds of cigarette smoke was developed. Within 10 min, 8 carbonyl compounds have been separated and identified on ZORBAX Eclipse XDB-C18 column ($4.6{\times}50\;mm$, $1.8\;{\mu}m$) with gradient elution using water and acetonitrile as a mobile phase. RRLC was used for the quantification of carbonyl compounds in mainstream smoke of 3R4F reference cigarette, and evaluated those efficiencies in the recovery, repeatability and reproducibility. The correlation coefficients ($r^2$) for calibration curves of carbonyl compounds were over 0.9998. The developed RRLC method was successfully applied to the analysis of smoke samples and the recoveries of carbonyl compounds were in the range of 97.5~102.1% with RSD<3.1%.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2014년도 추계학술대회 논문집
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• pp.110-115
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• 2014

The nuclear power plant was launched by Kori unit 1 in 1978 years. Currently, 23 nuclear power plants have been operating in Korea since 1978 years. The localization was completed for most of the reactor facility from Hanbit(Youngkwang) unit 3&4. However, RCP(Reactor Coolant Pump) and MMIS(Man Machine Interface System) is an important technology that has been excluded from the scope of the technical transfer has been dependent on a specific overseas vendor. Recent success in RCP development through co-operation with government and industries. Developed RCP will be applied to Shin-Hanul unit 1&2 nuclear power plants. The RCP operates in high speed and high pressure condition and only rotating component in the NSSS(Nuclear Steam Supply System). Therefore, the problem of vibration has arisen caused by the hydraulic forces of the working fluid. These forces can influence on the stability characteristics for entire RCS(Reactor Coolant System) loop, and can act as significant destabilizing forces. In this study, vibration evaluation of the pump shaft of development RCP estimated under normal operation and over speed conditions. In order to predict the vibration characteristics and dynamic behavior, modal analysis, critical speed analysis and unbalance response spectrum analysis were performed.

• 한국압력기기공학회 논문집
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• 제15권2호
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• pp.50-57
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• 2019

This paper investigates deformation characteristics of gas pipeline using the in-plane bending experiment and finite element analysis of a pipe bend. The effect of the bending angle and internal pressure on the deformation characteristics is analyzed. The pipe bend used in this study is API 5L X65 (out diameter: 20 inch) material with the thickness of 11.9 mm. The maximum load, displacement at maximum load, angle and local strain of 90° pipe bend are obtained from the in-plane bending experiment. Comparison between FE results and experimental data shows overall good agreements. In addition, the deformation characteristics of 22.5° and 45° pipe bend are calculated using the finite element analysis. As a result, the effect of the bend angle on the deformation characteristics is discussed.

• 한국연초학회지
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• 제30권1호
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• pp.33-38
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• 2008

This study was carried out to determine the analytical methods for heterocyclic amines(HAs) of the tobacco smoke by LC/MS/MS. HAs have been found in pyrolysate of protein and cooked food including protein, were known the Sugimura compound. HAs content of the smoke were known to exist very low ppb level. Especially, some of HAs are mutagenic and carcinogenic compounds. In according to IARC, the toxicity of N-heterocyclic amines classified IARC class 2A or 2B group. Precursors of these compounds are glutamic acid, protein and free amino acids including tryptophan, therefore, the precursors have been proved in cooked food continuously. This study was investigate multiple analysis methods for HAs and HAs contents of some commercial products. In this study, we used the linear type smoking machine for HAs analysis. At the ISO conditions, mainstream smoke was collected on cambridge filter pad, and then cambridge filter pad was extracted by 0.1% acetic acid. The extracted solution were passed cation exchange SPE cartridge to remove matrix, samples were analyzed using LC/MS/MS on MRM mode. From the result that optimized this methods, the correlation coefficient(R) of the individual compounds were good linearity over 0.999, recovery rate over 96% and the limit of detection were good values between 0.06 to 0.37 ng/mL, In addition, HAs content of some commercial products were in range of 0.02 to 43.8 ng/cig.

• 한국가스학회지
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• 제24권4호
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• pp.10-17
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• 2020

신뢰도 기반 평가법은 천연가스배관의 기하학적 형상 변화, 기계적 특성, 하중변화 및 운영조건을 평가 인자로 사용하여 천연가스배관의 건전성 평가 관리의 신뢰도를 향상시킬 수 있다. 구조신뢰성 평가 시 배관의 파손확률은 외부하중에 대한 배관재료의 저항성과의 관계에 의해 평가된다. COMREL 프로그램을 사용하여 내압, 열응력 및 굽힘응력과 같은 복합응력에 의한 천연가스배관의 파손확률을 평가했다. 천연가스배관의 파손확률 평가 시 매설깊이는 1.5~30m, 차량바퀴하중은 2.5~20톤, 온도차는 45℃, 운전압력은 6.86MPa 그리고 토양밀도는 1.8kN/㎥를 사용하였다. 천연가스배관의 파손확률은 Von-Mises 응력 기준에 의해 복합응력 하의 최대허용응력 기준으로 평가하였다.

• IMMUNE NETWORK
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• 제4권1호
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• pp.16-22
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• 2004

Background: To develop a novel treatment strategy for hepatitis B virus infection, a major cause of liver chirosis and cancer, we aimed to make human monoclonal antibodies inhibiting RNase H activity of P protein playing in important role in HBV replication. In this regard, phage display technology was employed and demonstrated as an efficient cloning method for human monoclonal antibody. So this study analysed the usability of human monoclonal antibody as protein based gene therapy. Methods: RNase H of HBV was expressed as fusion protein with maltose binding protein and purified with amylose resin column. Single chain Fv (scFv) phage antibody library was constructed by PCR cloning using total RNAs of PBMC from 50 healthy volunteers. Binders to RNase H were selected with BIAcore 2000 from the constructed library, and purified as soluble antibody fragment. The affinity and sequences of selected antibody fragments were analyzed with BIAcore and ABI automatic sequencer, respectively. And finally RNase H activity inhibiting assay was carried out. Results: Recombinant RNase H expressed in E. coli exhibited an proper enzyme activity. Naive library of $4.46{\times}10^9cfu$ was screened by BIAcore 2000. Two clones, RN41 and RN56, showed affinity of $4.5{\times}10^{-7}M$ and $1.9{\times}10^{-7}M$, respectively. But RNase H inhibiting activity of RN41 was higher than that of RN56. Conclusion: We cloned human monoclonal antibodies inhibiting RNase H activity of P protein of HBV. These antibodies can be expected to be a good candidate for protein-based antiviral therapy by preventing a replication of HBV if they can be expressed intracellularly in HBV-infected hepatocytes.

• 대한기계학회논문집A
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• 제41권11호
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• pp.1021-1033
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• 2017

본 연구에서는 매설가스배관의 강 슬리브 보수 용접부에 대한 피로수명에 미치는 보수 용접 공정 변수별 영향을 고찰하여 최적 공정을 수립하였다. 상용 유한요소 해석프로그램인 ABAQUS를 이용한 연계 연성 온도-응력 해석을 통해 강 슬리브 보수용접 시 보수용접부에 발생하는 용접 잔류응력을 도출하였다. 또한, 유한요소 선형탄성 응력해석을 통해 운전응력 변이도 도출하였다. 도출된 용접 잔류응력과 운전응력 변이를 입력값으로써 구조응력/파괴역학 접근법에 대입하여 강 슬리브 보수용접부의 피로수명을 평가하였다. 다양한 보수용접 공정 변수에 대한 유한요소해석과 피로평가를 수행하여 공정변수별 피로수명에 미치는 영향을 고찰하였다. 최종적으로는 고찰한 피로수명에 미치는 공정변수별 영항 결과를 토대로 공정 및 경제적 비용을 최소화하고 피로수명을 적절히 향상시킬 수 있는 최적 방안을 도출하였다.