• Journal of the Korean Institute of Intelligent Systems
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• v.26 no.5
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• pp.343-350
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• 2016

Remaining lifetime prediction of the underground gas pipeline plays a key role in maintenance planning and public safety. One of main causes in the pipeline failure is metal corrosion. This paper deals with estimating the pipeline reliability in the presence of corrosion defects. Because a pipeline has uncertainty and variability in its operation, probabilistic approximation approaches such as first order second moment (FOSM), first order reliability method (FORM), second order reliability method (SORM), and Monte Carlo simulation (MCS) are widely employed for pipeline reliability predictions. This paper presents a fuzzy inference based reliability method (FIRM). Compared with existing methods, a distinction of our method is to incorporate a fuzzy inference into quantifying degrees of variability in corrosion defects. As metal corrosion depends on the service environment, this feature makes it easier to obtain practical predictions. Numerical experiments are conducted by using a field dataset. The result indicates that the proposed method works well and, in particular, it provides more advisory estimations of the remaining lifetime of the gas pipeline.

• Journal of the Korean Society of Tobacco Science
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• v.31 no.1
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• pp.45-50
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• 2009

In this study, a simple gradient RRLC method for rapid determination of carbonyl compounds of cigarette smoke was developed. Within 10 min, 8 carbonyl compounds have been separated and identified on ZORBAX Eclipse XDB-C18 column ($4.6{\times}50\;mm$, $1.8\;{\mu}m$) with gradient elution using water and acetonitrile as a mobile phase. RRLC was used for the quantification of carbonyl compounds in mainstream smoke of 3R4F reference cigarette, and evaluated those efficiencies in the recovery, repeatability and reproducibility. The correlation coefficients ($r^2$) for calibration curves of carbonyl compounds were over 0.9998. The developed RRLC method was successfully applied to the analysis of smoke samples and the recoveries of carbonyl compounds were in the range of 97.5~102.1% with RSD<3.1%.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2014.10a
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• pp.110-115
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• 2014

The nuclear power plant was launched by Kori unit 1 in 1978 years. Currently, 23 nuclear power plants have been operating in Korea since 1978 years. The localization was completed for most of the reactor facility from Hanbit(Youngkwang) unit 3&4. However, RCP(Reactor Coolant Pump) and MMIS(Man Machine Interface System) is an important technology that has been excluded from the scope of the technical transfer has been dependent on a specific overseas vendor. Recent success in RCP development through co-operation with government and industries. Developed RCP will be applied to Shin-Hanul unit 1&2 nuclear power plants. The RCP operates in high speed and high pressure condition and only rotating component in the NSSS(Nuclear Steam Supply System). Therefore, the problem of vibration has arisen caused by the hydraulic forces of the working fluid. These forces can influence on the stability characteristics for entire RCS(Reactor Coolant System) loop, and can act as significant destabilizing forces. In this study, vibration evaluation of the pump shaft of development RCP estimated under normal operation and over speed conditions. In order to predict the vibration characteristics and dynamic behavior, modal analysis, critical speed analysis and unbalance response spectrum analysis were performed.

• Transactions of the Korean Society of Pressure Vessels and Piping
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• v.15 no.2
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• pp.50-57
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• 2019

This paper investigates deformation characteristics of gas pipeline using the in-plane bending experiment and finite element analysis of a pipe bend. The effect of the bending angle and internal pressure on the deformation characteristics is analyzed. The pipe bend used in this study is API 5L X65 (out diameter: 20 inch) material with the thickness of 11.9 mm. The maximum load, displacement at maximum load, angle and local strain of 90° pipe bend are obtained from the in-plane bending experiment. Comparison between FE results and experimental data shows overall good agreements. In addition, the deformation characteristics of 22.5° and 45° pipe bend are calculated using the finite element analysis. As a result, the effect of the bend angle on the deformation characteristics is discussed.

• Journal of the Korean Society of Tobacco Science
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• v.30 no.1
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• pp.33-38
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• 2008

This study was carried out to determine the analytical methods for heterocyclic amines(HAs) of the tobacco smoke by LC/MS/MS. HAs have been found in pyrolysate of protein and cooked food including protein, were known the Sugimura compound. HAs content of the smoke were known to exist very low ppb level. Especially, some of HAs are mutagenic and carcinogenic compounds. In according to IARC, the toxicity of N-heterocyclic amines classified IARC class 2A or 2B group. Precursors of these compounds are glutamic acid, protein and free amino acids including tryptophan, therefore, the precursors have been proved in cooked food continuously. This study was investigate multiple analysis methods for HAs and HAs contents of some commercial products. In this study, we used the linear type smoking machine for HAs analysis. At the ISO conditions, mainstream smoke was collected on cambridge filter pad, and then cambridge filter pad was extracted by 0.1% acetic acid. The extracted solution were passed cation exchange SPE cartridge to remove matrix, samples were analyzed using LC/MS/MS on MRM mode. From the result that optimized this methods, the correlation coefficient(R) of the individual compounds were good linearity over 0.999, recovery rate over 96% and the limit of detection were good values between 0.06 to 0.37 ng/mL, In addition, HAs content of some commercial products were in range of 0.02 to 43.8 ng/cig.

• Journal of the Korean Institute of Gas
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• v.24 no.4
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• pp.10-17
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• 2020

The structural reliability assessment can be used to improve the reliability in the asset integrity management of the pipeline by using a geometric variation, mechanical characteristics, load change and operating condition as evaluation factors. When evaluating structural reliability, the failure probability of the natural gas pipe is evaluated by the relationship of the resistance of the pipe material to external loads. The failure probability of the natural gas pipe due to the combined stresses such as the internal pressure, thermal stress and bending stress was evaluated by using COMREL program. When evaluating the failure probability of the natural gas pipe, a buried depth of 1.5 to 30 m, a wheel load of 2.5 to 20 ton, a temperature difference of 45℃, an operating pressure of 6.86MPa, and a soil density of 1.8 kN/㎥ were used. The failure probabilities of the natural gas pipe were evaluated by the Von-Mises stress criterion as the maximum allowable stress criterion under the combined stresses.

• IMMUNE NETWORK
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• v.4 no.1
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• pp.16-22
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• 2004

Background: To develop a novel treatment strategy for hepatitis B virus infection, a major cause of liver chirosis and cancer, we aimed to make human monoclonal antibodies inhibiting RNase H activity of P protein playing in important role in HBV replication. In this regard, phage display technology was employed and demonstrated as an efficient cloning method for human monoclonal antibody. So this study analysed the usability of human monoclonal antibody as protein based gene therapy. Methods: RNase H of HBV was expressed as fusion protein with maltose binding protein and purified with amylose resin column. Single chain Fv (scFv) phage antibody library was constructed by PCR cloning using total RNAs of PBMC from 50 healthy volunteers. Binders to RNase H were selected with BIAcore 2000 from the constructed library, and purified as soluble antibody fragment. The affinity and sequences of selected antibody fragments were analyzed with BIAcore and ABI automatic sequencer, respectively. And finally RNase H activity inhibiting assay was carried out. Results: Recombinant RNase H expressed in E. coli exhibited an proper enzyme activity. Naive library of $4.46{\times}10^9cfu$ was screened by BIAcore 2000. Two clones, RN41 and RN56, showed affinity of $4.5{\times}10^{-7}M$ and $1.9{\times}10^{-7}M$, respectively. But RNase H inhibiting activity of RN41 was higher than that of RN56. Conclusion: We cloned human monoclonal antibodies inhibiting RNase H activity of P protein of HBV. These antibodies can be expected to be a good candidate for protein-based antiviral therapy by preventing a replication of HBV if they can be expressed intracellularly in HBV-infected hepatocytes.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.11
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• pp.1021-1033
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• 2017

In this study, an optimum process to improve structural integrity was established by investigating effect of the process variables on fatigue lifetime of steel-sleeve repair welds in buried gas pipeline. Residual stresses in the repair welds were derived through sequentially-coupled temperature-stress analysis using ABAQUS, which is a commercial finite element analysis program. In addition, variations of operating stresses were derived by finite element linear elastic stress analysis. Fatigue lifetimes of the steel-sleeve repair welds were evaluated by substituting the derived weld residual stresses and operating stress variations into the structural stress/fracture mechanics approach as input. Parametric study using finite element analysis and fatigue assessment for various repair welding process variables were carried out to investigate the effects of the process variables on the fatigue lifetime. Finally, based on the effects of the process variables on the fatigue lifetime, an optimal process to minimize the welding time and economic costs and to improve the fatigue lifetimes was derived.