• KSBB Journal
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• v.7 no.4
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• pp.265-270
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• 1992

Low cost fermentation substrates frequently contain a mixture of carbon sources including hexoses, pentoses and disaccharides. Fermentation of such mixtures requires an understanding of how each of these substrates is utilized. During batch culture of Pichia stipitis CBS 5776 on sugar mixtures, glucose causes catabolite repression of xylose and cellobiose utilization. Also, glucose causes a permanent repression of xylose utilization as evidenced by reduced growth rates during the xylose phase of glucose/xylose fermentation. The growth model for multiple substrates is developed based on a cyclic AMP mediated catabolite repression mechanism and this model adequately described the growth and ethanol production from sugar mixtures.

• Applied Biological Chemistry
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• v.35 no.6
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• pp.501-506
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• 1992

Pepsin-catalyzed hydrolysis and plastein reaction were carried out to prepare plastein product from soymilk residue protein. Conditions required for optimal hydrolysis of soymilk residue protein and subsequent plastein production were investigated. The optimum substrate concentration, enzyme-substrate ratio, pH, reaction temperature and incubation time for hydrolysis were 3%, 1/50, 1.7, $45^{\circ}C$ and 24 hours, respectively. Plastein formation from peptic hydrolysate of soymilk residue protein was most effective at substrate concentratin of 40%, pH 4 and $45^{\circ}C$. Reaction time of 18 hours and enzyme-substrate ratio of 1/100 were selected for plastein production. Electrophoresis of the products revealed that protein-like substances of high molecular weight were produced from the plastein reaction.

• Geotechnical Engineering
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• v.2 no.2
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• pp.17-28
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• 1986

This study was intended to investigate the consolidation characteristics of lowly organic soils and highly organic ones, with organic content 15 of and 68 % respectively. which were sampled from three different soil regions in Chonbuk province. The results were obtained partly from standard consolidation test but mostly from single increment consolidation test in which each sample was held under the first sustained load for weeks. Highly organic soils retained considerably larger void ratio than lowly organic ones. Decrease of void ratio due to load increment was gradual in lowly organic soils and abrupt in highly organic ones. The long-term compression quantity of the highly organic soils became linearly proportional to the logarithm of time after 5 minutes. The lowly organic soils showed a compression pattern similar to that of clay. For highly organic soils, the secondary consolidation coefficient appeared to have a constant relationship with the logarithm of consolidation time, and therefore may be used as a significant factor in estimating the long-term settlement.

• Journal of the Korean Institute of Educational Facilities
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• v.21 no.6
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• pp.28-32
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• 2014

본 투고에서는 2008년 환경부의 용역으로 실시된 "보육시설의 실내공기질 진단 및 개선 시범사업"의 결과를 바탕으로 우리나라 보육시설의 실내공기 오염 실태와 함께 개선방안, 그리고 개선효과에 대하여 소개하였다. 서울, 경기, 인천 지역의 61개 시설을 대상으로 실내공기질 측정과 설문조사를 실시한 결과 폼알데하이드와 총 휘발성유기화합물은 일부시설에서 "다중이용시설 등의 실내공기질 관리법" 기준치를 초과하는 것으로 나타났다. 반면 이산화탄소와 미세먼지는 조사 대상 시설 중 50 % 이상이 기준치를 초과하는 것으로 나타났다. 따라서 보육시설의 실내공기질 개선을 위해서는 각 오염물질의 발생원별로 유지관리 대책이 필요한 것으로 조사되었다. 또한 오염원인별 분석결과, 대부분 시설의 실외오염물질 농도가 실내보다 낮게 나타나 외기의 오염이 실내에 미치는 영향은 미비한 것으로 나타났으며, 계절에 따른 실내공기오염은 동절기에 낮은 외기온도로 인하여 실내를 하기 때문에 하절기에 비해 더 높게 나타났다. 공간에 따른 오염물질의 농도는 이산화탄소와 미세먼지의 경우 아동이 늘 상주하는 교실의 오염이 가장 심한 것으로 나타났으며 폼알데하이드와 총휘발성유기화합물의 경우 놀이실의 오염도가 교실에 비해 더 높게 나타났다. 개선방안별 개선효과에 있어서는 환기계획 몇 환기설비 설치, 지하공간 습기 문제 개선에서 가장 높은 효과가 나타났다. 향후 보육시설의 쾌적하고 건겅한 실내환경의 유지관리를 위해서는 보육종사자들이 활용할 수 있는 유지관리 지침 또는 매누얼에 대한 보급이 필요할 것으로 판단된다.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.16 no.3
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• pp.117-122
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• 2016

제1형 고셔병(Gaucher disease type 1)은 리소좀 효소인 산성 ${\beta}$-글루코시다아제(acid ${\beta}-glucosidase$)의 결핍으로 인한 리소솜 축적 질환이다. 효소 활성도가 감소되어 기질이 축적되어, 간비종대, 빈혈, 혈소판감소증 및 골질환을 포함한 전신 증상이 발생한다. 재조합 효소 단백을 정맥 주입하는 효소 대체요법(Enzyme replacement therapy)는 지난 20년 넘게 고셔병의 표준 치료법이었다. 그러나 성공적인 효소 대체요법에도 불구하고, 심각한 폐증상과 골격 증상 등 고셔병 치료에 여전히 해결되지 않는 문제들이 남아 있다. 기질 감소 치료(Substrate reduction therapy)는 기질의 생합성을 억제하여 축적을 감소시킨다. 최근 새로운 경구용 기질감소 치료제인 엘리글루스타트(eliglustat)가 적합한 CYP2D6 대사 표현형을 가진 고셔병 성인 환자를 위한 1차 치료제로 미국과 유럽에서 승인되었다. 엘리글루스타트가 아직 한국에서는 쓰이지 않고 있지만, 본 종설에서는 문헌 검토를 통해 고셔병의 새로운 치료로서의 효소 대체요법을 소개하고자 한다. 아직 확고한 결론을 도출하기에는 연구 결과가 제한적이기는 하지만, 현재까지의 데이터에 따르면 엘리글루스타트는 임상 효능에 있어서 효소 보충 요법에 비열등성을 보인다. 장기 결과에 대한 추가 연구가 필요하지만, 엘리글루스타트의 승인은 해당 1형 고셔병 성인 환자들에게 경구 치료제라는 새로운 선택을 가능하게 하였다. 향후 국내에서 엘리글루스타트가 처방 가능해 지면, 각 환자 마다 철저한 평가를 통해 치료법을 선택할 수 있도록 해야 할 것이다. 나아가, 국내 1형 고셔병 환자들을 위해 엘리글루스타트의 사용에 관한 임상적 지침 또한 조만간 개발될 필요가 있다.

• Applied Biological Chemistry
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• v.40 no.3
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• pp.196-201
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• 1997

Assuming that Asp225 is the substrate specificity determinant of Achromobacter pretense I (APl) which is lysine-specific serine protease, the 225th residue was substituted for other amino acids with a hope that the substrate specificity of a mutant API is altered. Furthermore, to maturate preform of mutant API autocatalytically, Lys(-1) was also replaced by Met, Asp, or Glu. However, all the mutants were not expressed, or accumulated as inactive precursor proteins. This result implicats that Asp225 plays a critical rol in restricted substrate specificity as a lysylendopeptidase but the substrate specificity of API is not determined only by the nature of residue 225.

• Microbiology and Biotechnology Letters
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• v.16 no.5
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• pp.427-431
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• 1988

The simultaneons mineralization of aniline and other secondary carbon sources by Pseudomonas testosteroni 6Fl were evaluated by the lag time and the enzyme induction level. The lag time for aniline mineralization by P. testosteroni 6Fl was 7 hours, whereas the lag time for aniline and readily utilizable secondary substrates were 1-3 hours. This stimulated degradation resulted from the simultaneous use of secondary substrates and aniline, the increased rate of enzyme induction, and the in-creased rate of the cell growth. The enzyme induction level of P. testosteron 6F1 were varied according to the kinds of secondary substrate.

• Microbiology and Biotechnology Letters
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• v.19 no.5
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• pp.514-520
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• 1991

Direct synthesis of cyclodextrin (CD) from extruded insoluble corn starch without liquefaction procedure using cyclodextrin glucanotransferase (CGTase) was carried out. Increased CD production rate and yield were achieved in heterogeneous enzyme reaction system containing extruded corn starch compared with those of conventional system employing liquefied or partially cyclized starch. At extruded starch concentration of 100 g/l the CD concentration and conversion yield were reached up to 54 g/l and 0.54, respectively. High purity of $\alpha \beta \gamma$-CDs without accumulation of undesirable malto-oligosaccharides was produced, furthermore, the residual extruded starch was easily separated by centrifugation from reaction mixture, whlch will facilitate the purification procedure. Granular structure of extruded starch was observed by SEM to investigate enzyme reaction mechanism. Supplemental addition of $\alpha$-amylase enhanced slightly the initial CD production rate, but it decomposed produced CD at the late stage. Various! extruded raw starches, such as, corn, rice, and barley were also suitable substrates for CD production.

• KSBB Journal
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• v.9 no.2
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• pp.122-126
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• 1994

A new method for the production of isomalto-oligosaccharides from maltose was investigated using intact cells of Aureobasidium pullulaans which had been known to produce fructo-oligosaccharides. The cells showed transglucosylation activity producing isomalto-oligosaccharides at high concentrations of maltose, while they showed a hydrolytic activity at low concentrations of substrate when cultivated at $25^{\circ}C$. The optimum reaction conditions for the isomalto-oligosaccharide production were as follows: substrate concentration, 500g/l maltose; pH, 4.5; temperature, $65^{\circ}C$; cell dosage, 10 unit per gram substrate. Under optimized conditions, the maximum yield of isomalto-oligosaccharides achieved was around 48% (w/w). At the early period of reaction, panose was selectively produced from maltose, and thereafter isomaltotriose was synthesized by utilizing panose as a substrate when maltose consumption was discontinued.

• Polymer Science and Technology
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• v.15 no.2
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• pp.191-197
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• 2004

실내 환경 (indoor environment)에 대한 관심은 1990년대 이후 급격한 산업구조의 발달을 통한 에너지 소비가 급증하여 환경오염이 가중되면서 본격적으로 대두되었다고 할 수 있다. 실내 환경문제의 대표적인 실내공기질 (Indoor Air Quality, IAQ)에 대한 문제의 발생배경을 보면 1970년대 이후 각종 산업분야에서 에너지 절감 및 효율을 높이기 위한 노력의 일환으로 건물의 열효율을 위한 밀폐화 및 고기밀화에 따른 실내공간의 변화에 따라 이들 건물의 실내공기질이 악화되면서 발생되었다.(중략)