• Horticultural Science & Technology
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• v.17 no.2
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• pp.138-140
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• 1999

These studies were carried out to investigate the effects of cold pretreatment, dark condition, and Ficoll on the callus formation and organ differentiation in anther culture of carnation for the purpose of settling the techniques of anther culture of 'Rony' carnation. Diameter of flower buds containing anthers with microspores before or after uniuncleated stage was between 4 and 6 mm. A high percentage of callus formation and responding anthers were achieved by low temperature pretreatment at $4^{\circ}C$ for 7 days. Callus formation was promoted under dark condition. Roots were differentiated from calli formed under darkness. Ficoll didn't promote callus formation but promoted differentiation of shoots and roots.

• Korean Journal of Medicinal Crop Science
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• v.15 no.1
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• pp.62-66
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• 2007

Plant Regeneration via organogenesis from leaf disk of Korean dandelion was investigated. Leaf disk cultured on MS medium with various combinations of BA (0-4 mg/L) and 2,4-D (0-1 mg/L). Shoot regeneration from leaf explant was observed after 3 weeks of culture. The highest shoot regeneration frequency from leaf disk was obtained with 2 mg/L BA. To analyze the effect of leaf age along shoot formation, we measured number of shoots per explant, shooting rate, fresh and dry weight of leaf explant. The highest number of shoots (11.5) per explant were obtained leaf from 7 weeks old plantlets after seed germination. The regenerated shoots were transferred in 1/2 MS medium with 0.5 mg/L NAA for root formation. Regeneratied plantlets thought organogenesis were growing to whole plants in the pots with acclimation.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.14-20
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• 1997

To increase the efficiency of the callus induction in anther culture of Schizandra chinensis, the effects of culture stage, low temperature pretreatment, growth regulators, sucrose and gelling agents were tested on Murashige and Skoog's medium. And the effects of ABA and $AgNO_3$ on organogenesis were investigated. The most suitable stage for anther culture was at the middle to late uninucleate stage, of which the flower size was $6.2{\pm}0.6{\times}4.0{\pm}0.4mm(Length{\times}width)$, and the frequency of callus induction was 13.3%. The effect of low-temperature pretreatment on callus induction was highest as 12.5% in the trearment for 8 days at $4^{\circ}C$. The combination of 1.0 mg/L 2, 4-D and 0.25 mg/L kinetin for callus induction was most effective as 8.3% among various media. The frequency of callus induction was excellent as 10.8% in 4% sucrose. Effect of gelling agents on callus induction was highest as 9.0% on 0.6% Gelrite medium. The prevention of callus browning was effective on the media supplemented with ABA and $AgNO_3$ and rooting was promoted on medium supplemented with 5 mg/L ABA. But shoot was not developed.

• The Journal of the Korea Contents Association
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• v.18 no.4
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• pp.60-76
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• 2018

The objective of this study was to determine the effects of self-differentiation and ego-resilience on service maladjustment behaviors of Social Service Agent. To achieve this, we chose these research questions. To verify these research questions, data were collected by distributing 470 questionnaires to Social Service Agent serving in service organizations located in Seoul. Four hundred twenty-seven surveys were used for statistical analysis. The results of this study are as follows: First, Social Service Agents were shown to generally adjust well to service, as self-differentiation and ego-resilience were slightly higher than the median, while service maladjustment behavior was slightly lower than the median. Second, when the effects of sociodemographic characteristics, self-differentiation and ego-resilience on service maladjustment behavior were studied, all values of sociodemographic characteristics, self-differentiation and ego-resilience affected all lower factors of service maladjustment behavior with significance. For social withdrawal, the effectiveness of interpersonal relations, optimistic attitude, and the degree of family regression had a negative correlation, and emotional divorce, education level, and term of service had a positive correlation. The degree of family regression, anger management, and cognitive function vs. emotional function had a negative correlation with hyperactivity while self unity had a positive one. Family regression, cognitive function vs. emotional function, anger management, and family's economic level negatively correlated with aggression. Family regression, optimistic attitude, cognitive function vs. emotional function, family's economic level and term of service had a negative correlation with obsession and compulsion.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.69-73
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• 2007

A reliable and effective tissue culture system was established for Perilla frutescens (perilla) using different cultivar, explant source, and growth regulator composition in medium. MS medium supplemented with 2.0 mg/L BA and 0.1 mg/L NAA was maximum at shoot induction. Cotyledon explants formed more shoots than hypocotyl explants. The frequency of plant regeneration through organogenesis was higher (22.8%) than that (6.1%) of somatic embryogenesis. Five genotypes of perilla were screened for the feasibility of shoot regeneration, cotyledon explant of 'Manbaek' showed the highest shoot induction at a frequency of 27.3% among the tested cultivars.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.265-266
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• 2000

자주복, Takifugu rubripes은 경골어류 복어목 참복과에 속하고, 우리 나라 전 연안 및 일본 훗카이도 이남, 중국 등지에 분포하며 전장 70 cm이상까지 성장하는 대형어이다(Abe, 1949). 어류는 자어기에 내부영양에서 외부영양으로 영양원이 전환되는 초기생활사에 있어 소화 기관계의 변화가 일어나고, 치어기의 생장과 생존에 크게 영향을 미친다고 제시하고 있다(Lee, 1988). 이 연구는 소화흡수와 직접 관련된 자주복 부화 자·치어의 각 성장단계에 따른 소화 기관의 형태적 기능적 분화과정을 조직학적으로 관찰하여, 종묘생산 과정에서의 초기 대량 폐사를 줄이고, 적절한 먹이 투여시기에 의한 보다 나은 성장률을 얻는데 중요한 기초자료를 제공하고자 한다. (중략)

• Journal of Sericultural and Entomological Science
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• v.30 no.1
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• pp.1-7
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• 1988

Flower buds of the mulberry(Morus alba L., Morus bombycis Koidz.) were cultured under different conditions such as basal media, and various concentrations of plant growth substances. Effects of the culture conditions on growth of the buds and organ regeneration were investigated and the result obtained are as follows: Murashige and Skoog(M.S.) medium was more effective on budding and growth of female(Keomseolppong) and male(Kaeryangppong) flower buds isolated directly from branches, compared to Greshoff & Doy(G.D.) and Wolter & Skog(W.S.) media. The growth of the female buds was promoted at higher concetration of benzyl amino purine(BAP) i.e., 2.0ppm. The female and male buds cultured after cuuting for seven days showed better growth than those without cutting treatment. The females and the males bloomed to form healthy stigmas and anthers, respectively, when cultured on M.S. media containing high Kinetin with low concentration of indole acetic acid(IAA).

• Journal of Chest Surgery
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• v.31 no.10
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• pp.1014-1016
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• 1998

Rupture of the bronchus following endotracheal intubation with a double-lumen tube is extremely rare in all complications of endotracheal intubation. We experienced a case of left main bronchial rupture following endotracheal intubation. This 58-year old female patient was diagnosed of well-differentiated adenocarcinoma of right lower lobe, stage IIB, preoperatively. She was intubated with Robertshaw double-lumen tube(35 Fr.) for Rt. lower lobectomy. Intraoperatively, Lt. main bronchial rupture was suspected because of pneumomediastinum and ventilation insufficiency and immediately repaired with monofilament absorbable sutures(PDS) through left thoracotomy. Postoperative course was uneventful.

• Journal of Chest Surgery
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• v.32 no.1
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• pp.66-69
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• 1999

Bronchogenic cyst is an uncommon congenital lesion which is derived from the primitive foregut. Most bronchogenic cyst may develope at the tracheal bifurcation, both main bronchi, the lung parenchymeand the mediastinum. A 40-year old male was evaluated for dyspnea and chest tightness. Computed tomography revealed a well dermarcated, 7.2 ${\times}$ 7.9 cm sized, homogeneous mass compressing the left atrium. 2D-echo showed grade III mitral regurgitation. We completely removed the cystic mass and then confirmed the bronchogenic cyst in the pathological diagnosis. During the follow up period, the patient progressed well without any symptoms and showed grade I mitral regurgitation on the 2D-Echo. Therefore, we report a case of the bronchogenic cyst causing grade III mitral regurgitation.

• Journal of Korean Society of Forest Science
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• v.84 no.2
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• pp.145-150
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• 1995

A plant regeneration system using shoot basal callus of in vitro cultured black locust(Robinia pseudoacacia L.) was established. Shoot basal callus was induced on MS medium supplemented with BA, or NAA, and mere more proliferated on BA containing medium than NAA containing medium at both light and dark conditions. Shoot basal callus was induced during shoot multiplication procedure. Two types of callus, green colored callus and whitish-yellow colored callus, were cultured on mMS medium containing 2.0 mg/l BA and 0.5 mg/l NAA. Green colored callus showed the shoot regeneration ability while whitish-yellow callus failed to regenerate shoot and died. Regenerated shoot were rooted on hormone-free ${\frac{1}{2}}MS$ medium within 2 weeks.