• Title/Summary/Keyword: 글루코스

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On-line Monitoring of Glucose and Acetate by Flow-Injection Analysis in Escherichia coli Fermentation Process (대장균 발효공정에서 흐름주입분석을 이용한 글루코스와 초산의 온라인 모니터링)

  • 이종일
    • KSBB Journal
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    • v.13 no.3
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    • pp.244-250
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    • 1998
  • Flow-injection analysis (FIA) for on-line monitoring of glucose and acetate are described and employed in E.coli fermentation process. Glucose oxidase (GOD) for the detection of glucose is immobilized on epoxy polymer support, which is packed in a small cartirdge, and applied to a GOD-FIA system. The detection of acetate is based on the inhibition of acetate to the oxidation of sarcosine by sarcosine oxidase (SOD). SOD is also immobilized on epoxy polymer support and used for a SOD-FIA system. GOD-FIA system is characterized as well as SOD-FIA system by the investigation of the effects of pH, temperature and metabolites in samples on the peak height. GOD-FIA and SOD-FIA systems were also applied for on-line On-line measurements buy FIA measurements by FIA were in god agreement with off-line measurements.

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Effect of Fabrication Method of Anode on OCV in Enzyme Fuel Cells (효소연료전지의 Anode 제조조건이 OCV에 미치는 영향)

  • Kim, Young-Sook;Lee, Se-Hoon;Chu, Cheun-Ho;Na, Il-Chai;Lee, Ho;Park, Kwon-Pil
    • Korean Chemical Engineering Research
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    • v.53 no.1
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    • pp.6-10
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    • 2015
  • Enzyme fuel cells were composed of enzyme anode and PEMFC cathode. Enzyme anodes was fabricated by compression of a mixture of graphite particle, glucose oxidase as a enzyme and ferrocene as a mediator, and then coated with Nafion ionomer. Open circuit voltage (OCV) were measured with variation of anode manufacture factors, to find optimum condition of enzyme anode. Optimum pressure was 9.0 MPar for enzyme anode pressing process. Highest OCV was obtained at 60% graphite composition in enzyme anode. Optimum glucose concentration was 1.7mol/l in anode substrate solution and enzyme activity of anode was stable for 7 days.

Ring type non-invasive glucose sensor (민감도가 향상된 링 형태의 비 침습식 혈당 센서)

  • Lee, Ki-Hyuck;Lee, Kook-Joo;Lee, Dong-Ho;Kim, Moon-Il;Park, Gil-Hong
    • Journal of IKEEE
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    • v.11 no.4
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    • pp.293-296
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    • 2007
  • A novel sensitive and non-invasive ring-type glucose probe was designed and measured. The magnitude and phase of return loss change with different concentration of the glucose solution inside the loop structure. Ring type probe is more advantageous than open ended probe to measure electrical characteristics under the skin. The maximum difference of return loss was 0.94 for the concentration difference of 0% and 20% at 285MHz and more sensitive in low frequency.

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Studies on Physical Behavior of Alkyl Polyglucosides (I) - Interfacial Activities and Detergency - (Alkyl Polyglucoside 계면활성제의 물리적 거동에 관한 연구 (I) - 계면활성과 세정력에 관하여 -)

  • Yoon, Yeo-Kyung;Choi, Kyu-Suk
    • Applied Chemistry for Engineering
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    • v.5 no.3
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    • pp.451-456
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    • 1994
  • Alkyl polyglucosides (APG), so called natural nonionic surfactants, are prepared with glucose from corn starch and fatty alcohol from palm and/or coconut oil. When we studied on interfacial properties of APG, surface and interfacial tension, cmc, foaming and effectiveness, according to degree of polymerization of glucose unit (D.P.=1.2~1.8) and alkyl chain length, the former hardly affects but the latter greatly affects on them. The order of detergency for fatty acid soils decreased as followings ; APG 0814> APG 1214> APG 10> APG 0810> APG 08, and the samples with lowest interfacial tension and maximum adsorption had a better detergency.

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Hydrogen Fermentation of the Galactose-Glucose Mixture (갈락토스-글루코스 혼합당 수소 발효)

  • Cheon, Hyo-Chang;Kim, Sang-Youn
    • Transactions of the Korean hydrogen and new energy society
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    • v.23 no.4
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    • pp.397-403
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    • 2012
  • Galactose, an isomer of glucose with an opposite hydroxyl group at the 4-carbon, is a major fermentable sugar in various promising feedstock for hydrogen production including red algal biomass. In this study, hydrogen production characteristics of galactose-glucose mixture were investigated using batch fermentation experiments with heat-treated digester sludge as inoclua. Galactose showed a hydogen yield compatible with glucose. However, more complicated metabolic steps for galactose utilization caused a slower hydrogen production rate. The existence of glucose aggravated the hydrogen production rate, which would result from the regulation of galactose-utilizing enzymes by glucose. Hydrogen produciton rate at galactose to glucose ratio of 8:2 or 6:4 was 67% of the production rate for galactose and 33% for glucose, which could need approximately 1.5 and 3 times longer hydraulic retention time than galacgtose only condition and glucose only condition, respectively, in continuous fermentation. Hydrogen production rate, Hydrogen yield, and organic acid production at galactose to glucose ratio of 8:2 or 6:4 were 0.14 mL H2/mL/hr, 0.78 mol $H_2$/mol sugar, and 11.89 g COD/L, respectively. Galactose-rich biomass could be usable for hydogen fermenation, however, the fermentation time should be allowed enough.

The effect of osmotic pressure in W/O/W multiple emulsion (다상 에멀전 형성에 있어 삼투 현상이 미치는 영향)

  • 신중진;이근수;강기춘;표형배
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.26 no.1
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    • pp.139-148
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    • 2000
  • The osmotic pressure is a one of the most important factor affecting stabilization of multiple emulsion in a law hours after experiment. To understand and decrease osmotic pressure between Wl phase and W2 phase, a kinds of humectants were introduced in outer water phase. As a result, Betaine and Glucose had an excellent effect reducing osmotic pressure and NaCl made W/O/W emulsion more stable than MgSO4 did when introduced in inner water phase.

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Effects of Regular Walking Exercise on Health-Related Parameters in Persons with Chronic Diseases (규칙적인 걷기운동이 질환별 노인의 건강변인에 미치는 효과)

  • Lim, Hwe-Jin;Kim, Yu-Sik;Cho, Hyeong-Suk;Kim, Chang-Hee;Lim, Hee-Jung;Jeong, Hee-Seong;Im, Jee-Aee;Yoon, Byung-Kon;Paik, Il-Young;Suh, Sang-Hoon
    • Journal of Life Science
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    • v.19 no.12
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    • pp.1750-1757
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    • 2009
  • The purpose of this study was to investigate the effects of regular walking exercise on health-related parameters in the elderly with chronic diseases - apoplexy, overweight, impaired fasting glucose, and overweight + impaired fasting glucose. A total of 85 subjects, 27, 17, 21 and 20 in apoplexy (A), overweight (O), impaired fasting glucose (IFG), overweight + impaired fasting glucose group (O_IFG), respectively, completed a 12-week walking exercise. The health-related parameters were measured before and at the completion of the exercise program including anthropometric measurements, functional physical fitness levels, blood pressure, fasting glucose, blood lipid profiles and chronic inflammatory markers (CRPs). Significant improvements in body weight, BMI, %body fat, blood pressure, all blood lipid measurements and all measured physical fitness items were shown in A; those in %body fat, TC, HDL-C and LDL-C in O; those in body weight, BMI, %body fat, fasting glucose, TC, TG and HDL-C in IFG; and those in body weight, HDL-C and LDL-C in O_IFG (p<0.05). The results of the present study demonstrated that a 12-week walking exercise brought positive effects on body weight, bloody lipid profiles, fasting glucose and functional physical fitness levels in the elderly with chronic diseases. In conclusion, this study suggested that walking regularly is very effective in lowering the risks of developing chronic diseases.

A Study on the Analysis of Risk Factors and Correlations of Coronary Artery Disease of the Examinee taking Coronary Computed Tomography Angiography in a Comprehensive Health Improvement Center (종합검진 수검자의 관상동맥 전산화단층 혈관조영술 검사에서 관상동맥질환의 위험요인과 관련성 분석)

  • Choi, Min-Gyeong;Gwak, Jong-Hyeok;Kim, Gwang;Lee, Sam-Yol
    • Journal of the Korean Society of Radiology
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    • v.13 no.7
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    • pp.1005-1014
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    • 2019
  • The purpose of this study is to evaluate the usefulness of coronary computed tomography angiography of coronary artery disease for the purpose fo health screening according to gender and age. In addition the association between hematological factors (Glucose, total cholesterol, visceral fat, body mass index, triglycerides, high density lipoprotein (HDL), low density lipoprotein (LDL)) and coronary artery disease is investigated. A retrospective analysis of coronary computed tomography angiography with 299 subjects showed that the detection rate of coronary artery disease was higher in men over 50 years of age and it was statistically significant. In particular, the prevalence rate of men (37.9%) was about 2 times higher than that of women (17.0%). Glucose and HDL as hematological factors associated with coronary artery disease were statistically significant. The prevention and management of coronary artery disease seems to require the control of glucose and high density lipoprotein (HDL). Although it is not statistically significant with other hematological factors, the need for management of coronary artery disease was identified. the coronary computed tomography angiography of coronary artery has higher radiation doses than other CT scans. Therefore, for the purpose of screening, coronary computed tomography angiography should be considered in consideration of the sex and age of the examinee, and detection of coronary artery disease through other non-invasive tests should be prioritized over coronary computed tomography angiography.

Environment-Friendly Synthesis of Graphene Ball and its Characterization (그래핀 볼의 친환경 제조 및 특성 평가)

  • Park, Su-Ryeon;Jo, Eun Hee;Kim, Sun Kyung;Chang, Hankwon;Jang, Hee Dong
    • Korean Chemical Engineering Research
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    • v.54 no.6
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    • pp.786-791
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    • 2016
  • Spherical graphene balls were fabricated by an aerosol spray drying process after reduced graphene oxide was prepared by the liquid phase reaction using glucose as an environment-friendly reducing agent. Spherical morphology of the as-fabricated particles was observed by FE-SEM analysis. Diffraction patterns of spherical particles were found as graphene by XRD analysis. Sphericity of GB was controlled by the variation of operating temperature, amount of glucose, and addition of $NH_4OH$. Higher sphericity of GB was prepared at higher operating temperature in the presence of $NH_4OH$. As the amount of glucose in the liquid phase reaction increased in the presence of $NH_4OH$, sphericity of GB increased. The highest sphericity of GB was 1.1. GB of higher sphericity showed lower aggregation property than that of lower sphericity. Furthermore, as-prepared GBs were found as a potential electrode material for capacitor.

Popping Pretreatment for Enzymatic Hydrolysis of Waste Wood (팝핑 전처리를 이용한 폐목재의 효소당화)

  • Kim, Hyun-Joo;Cho, Eun-Jin;Lee, Kwang-Ho;Kim, Su-Bae;Bae, Hyeun-Jong
    • Journal of the Korean Wood Science and Technology
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    • v.39 no.1
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    • pp.95-103
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    • 2011
  • We have investigated pretreatment of waste wood using milling refinery combined with poping method, which can save energy for pretreatment and enzyme loading for enzymatic hydrolysis. The chemical analysis of holocellulose of non and popping treated waste wood showed 65.9% and 58.8%, and the lignin, organic extracts and ash were increased by 3%, 4% and 0.7% after pretreatment, respectively. The reducing sugar yields of pretreated waste wood were increased four times more than non-pretreated one and the synergistic effect of cellulase and xylanase were evaluated compare with individual enzyme treatment. Especially, enzyme cocktail (cellulase 50 U and xylanase 50 U) treatment was very efficient in 1% substrate (50 mg). Also, glucose and xylose conversion rate of pretreated waste wood by GC analysis were 45.9% and 38.7%, respectively.