• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.429-444
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• 1998

Objective of this study was to select ectomycorrhizal fungi for black spruce(Picea mariana) inoculation to overcome the growth inhibitory effects of Kalmia angustifolia. Nineteen isolates representing 11 species of ectomycorrhizal fungi were tested for their abilities to grow and form mycorrhizae with black spruce seedlings in the presence of water leachate of leaves of Kalmia. Mycelium growth of 9 isolates were inhibited by the leaf leachate. Colony diameter and biomass of the other 10 isolates were either increased or unaffected under the same conditions. Acidic pH of the culture medium(pH 3 and 4) inhibited some of the fungi, but a combination of acidic pH and the leaf leachate was more inhibitory. Thirteen isolates were able to form ectomycorrhizae with black spruce in presence of 25% leaf leachate in pure culture. Four isolates, Paxillus involutus(NF4), Cenococcum geophilum(GB12), Laccaria laccata(GB23), and E-strain(GB45) formed mycorrhizae more successfully than the others in presence of up to 50% Kalmia leaf leachate. Black spruce seedlings pre-inoculated with these fungi were grown with Kalmia leaf leachate and live Kalmia plants during a four month greenhouse experiment. Abundant mycorrhizae(77-91% of root tips) were developed on seedlings pre-inoculated with P. involutus, L. laccata and E-strain but relatively poor mycorrhization(32% of root tips) resulted with C. geophidum. Over 90% of the short root mycorrhizae were attributed to the inoculated fungi although indigenous mycorrhizae also occurred on most seedlings. Persistence of the mycorrhizae was not affected by living Kalmia plants. Over 80% of the mycorrhizae on seedlings inoculated with P. involutus, L. laccata and E-strain and 53% of the mycorrhizae on seedlings inoculated with C. geophilum were attributable to the inoculant fungi. Control seedlings formed about 45% ectomycorrhizal short roots with indigenous fungi. The L. laccata and C. geophilum inoculated seedlings exhibited enhanced mycorrhizae formation in presence of Kalmia leaf leachate. Mycorrhizae formation with inoculant fungi was 4-15% lower at pH 4 than at pH 5, with the greatest inhibition occurring for L. laccata. Seedlings inoculated with P. involutus had the greatest shoot and root growth followed by L. laccata and E-strain inoculated seedlings. The P. involutus and L. laccata inoculated seedlings were significantly taller with more shoot dry biomass than the uninoculated(control) seedlings. E-strain inoculated seedlings had significantly higher shoot dry biomass and significantly lower number of first order lateral roots compared to the control but other growth parameters such as height, root dry weight and number of short root tips were not significantly different from the control. Seedlings inoculated with C. geophilum were not significantly different from the uninoculated seedlings in any of the growth parameters except for the number of first artier lateral roots which was significantly less than the control seedlings.

• The Korean Journal of Mycology
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• v.42 no.4
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• pp.306-311
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• 2014

In this study, arbuscular mycorrhizal fungi (AMF) were isolated and cultivated from rhizospheres of various plants in Korea. Five species belonging to four genus were identified based on spore morphology and 18S rDNA sequence analysis: Acaulospora cavernata Claroideoglomus luteum, Diversispora aurantium, D. trimurales, Rhizophagus irregularis. These fungal species have not been cultivated or reported to date in Korea, Morphological description and phylogenetic analysis for each species were presented. This result could be useful for research of AMF diversity in Korea.

• Korean Journal of Environment and Ecology
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• v.17 no.2
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• pp.144-152
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• 2003

The purpose of this study was to investigate the effects of lime fertilization and light intensity on beech regeneration strategy in a mature beech forest(ca. 150 years old) gaps, which was located in the soiling areas Germany, where the soil is an acid brown earth. For the experiment canopy gaps of 30m diameter were cut, and one canopy gap remained as untreated while the other was limed(3t dolomite h $a^{-1}$). Soil chemistry, soil water potential, light intensity(PAR), ground vegetation, beech seedlings and mycorrhiza were investigated along transect from the stands into gaps to the north. The results of this study are followings: 1) In the limed gap, soil chemistry was greatly improved and a vigorous herb layer developed as compared to the unlimed gap. 2) Development and growth of the beech seedlings in the unlimed gap were generally deficient. Specially, developments of lateral roots were very lack. 3) Through the lime fertilization, the minerals contents of beech leaves such as calcium(Ca) and magnesium(Mg) were increased, while manganese(Mn) was decreased. There was antagonism between potassium(K) and calcium(Ca) or magnesium(Mg). 4) The relative mycorrhiza frequency(RM) in beech seedlings of the southern edge was very higher than that of the middle part, and through the lime fertilization extension of the species Laccaria amethystina appeared clearly. The results of this study showed that lime fertilize in forest, where the soil is acid, will be necessary..

• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.311-328
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• 2017

This study was carried out to investigate the changes in soil microorganisms and soil enzymes by split irrigation and organic matter application under no-tillage green house conditions. Soil bacteria and fungi abundances were higher in soybean cake fertilizer than in the soil without the soybean cake fertilizer under whole quantity irrigation. Bacteria and fungi abundances in soil increased with increasing organic fertilizer application rate. Bacteria and fungi amount in the soil increased at half division irrigation in no-treatment of soybean cake fertilizer compared with whole quantity irrigation. Actinomycete amount in the soil decreased with increasing soybean cake fertilizer with whole quantity irrigation while clearly increased in no-treatment of soybean cake fertilizer. Actinomycete amount in soil clearly increased with increasing organic fertilizer input at half division irrigation. Chitinase activity in the soil decreased in soybean cake fertilizer with increasing organic fertilizer input, while increased in no-treatment of soybean cake fertilizer. Chitinase activity in the soil increased at half division irrigation compared with whole quantity irrigation regardless of soybean cake fertilizer input. ${\beta}$-Glucosidase activity in the soil was higher in soybean cake fertilizer than in no-treatment of soybean cake fertilizer with whole quantity irrigation. ${\beta}$-Glucosidase activity in the soil increased with increasing organic fertilizer input, but decreased in above the standard level 66%. ${\beta}$-Glucosidase activity in the soil clearly increased in no-treatment of soybean cake fertilizer at half division irrigation compared with whole quantity irrigation. N-acetyl-${\beta}$-D-glucosaminidase activity was higher in soybean cake fertilizer than in no-treatment of soybean cake fertilizer with whole quantity irrigation. N-acetyl-${\beta}$-D-glucosaminidase activity in the soil increased with increasing organic fertilizer input, but decreased in above the standard level 66%. N-acetyl-${\beta}$-D-glucosaminidase activity in the soil was not significantly different at half division irrigation and whole quantity irrigation in organic fertilizer input, while increased at half division irrigation in no-treatment of soybean cake fertilizer. Acid phosphatase activity increased at standard level 66% in soybean cake fertilizer, while was not significantly different in no-treatment of soybean cake fertilizer. Spore density of Arbuscular Mycorrhizal Fungi (AMF) in the soil increased with increasing organic fertilizer input at whole quantity irrigation in no-treatment of soybean cake fertilizer, while decreased above the standard level 66% in organic fertilizer input. However, spore density of AMF in the soil was not significantly different in soybean cake fertilizer regardless of input amount of organic fertilizer. Root colonization rate of AMF in red pepper roots was not significant difference at two irrigations regardless of soybean cake input.

• Journal of Ecology and Environment
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• v.29 no.3
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• pp.305-321
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• 2006

Atmospheric Acid Deposition: Nitrogen Saturation of Forests: Volume weighted annual average wet deposition of nitroge at 33 sites in Korea during 1999-2004 ranged 7.28 to $21.05kgN{\cdot}ha^{-1}{\cdot}yr^{-1}$ with average $12.78kgN{\cdot}ha^{-1}{\cdot}yr^{-1}$, which values are similar level with nitrogen deposition of Europe and North America. The temperate forests that suffered long-term high atmospheric nitrogen deposition are gradually saturated with nitrogen. Such nitrogen saturated forest watersheds usually leach nitrate ion ($NO_3^-$) in stream water and soil solution. It may be likely that Korean forest ecosystems are saturated by much nitrogen deposition. In leaves with nitrogen saturation ratios of N/P, N/K and N/Mg are so enhanced that mineral nutrient system is disturbed, suffered easily frost damage and blight disease, reduced fine-root vitality and mycorrhizal activity. Consequently nitrogen saturated forests decrease primary productivity and finally become forest decline. Futhermore understory species are replaced the nitrophobous species by the nitrophilous one. In soil with nitrogen saturation uptake of methane ($CH_4$) is reduced and emission of nitrogen monoxide (NO) and nitrous oxide ($N_{2}O$) are increased, which gases are greenhouse gas accelerating global warming.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.3
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• pp.165-172
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• 1973

The optimum time for nutritional diagnosis of the field-grown rice plant by total plant analysis, and the relationship between maximum or minimum nutrient content at various growth stages and corresponding yield and between maximum or minimum yield and corresponding nutrient content were as follows. 1. The percentage occurence of the minimum nutrient content in straw or grain of minus nutrient plot was in the order of 20 days after transplanting (20)>maximum tillering (MT)>harvested straw (HS)> earformation (EF)>straw at flowering (FS)>harvested grain (HG)>ear at flowering (FE) for nitrogen, MT>EF>HS>20=FS>FE>HG for phosphorus and MT>EF>20>FS>HG>FE for potassium. 2. The time when the occurece of minimum nutrient content in minus plot is highest was considered as the optimum time for nutritional diagnosis of root zone. It was 20 days after transplanting in N and maximum tillering stage in P and K. 3. The highest relative difference($100{\times}(L-H)/H$), between maximum (H)and minimum(L) nutrient content appeared in harvested straw for N and P while in harvested grain for K and Si, suggesting the close relation to their translocation from straw to grain. 4. The corresponding yield of maximum nutrient content was higher than that of minimum content at all growth stages in N, at all stages except MT and EF in P, at 20 days after trans planting and harvest in K, but it was always lower in Si, thus the contribution of nutrient content to yield will be in the order of N>P>K>Si. 5. The highest relative difference ($100{\times}(L-H)/H$, where H and L stand for yields) between yields corresponding to maximum and minimum nutrient content appeared at 20 days after transplanting for N. P. K, indicating the time of the closest relation between yield and nutrient content. 6. The highest difference (H-L, where H and L stand for nutrient content) between N. P. K contents corresponding to maximum or minimum yields came at 20 days after transplanting. The contents of N. P. K corresponding to the maximum total dry matter yield were lower than those corresponding the maximum grain yield at this stage. These facts support the closest relation between yield and nutrient content at this time. 7. The highest yield among yields corresponding to maximum nutrient contents occured at 20 days after transplanting in N. P. K but the lowest yield among yields corresponding to minimum nutrient contents appeared at the same stage only in nitrogen. 8. From the above facts the optimum time for diagnosis of nutrient around root zone seems different from that for diagnosis of nutritional status in relation to grain yield.