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Optimization of Interesterification Reaction for the Continuous Production of trans-Free Fat in a Packed Bed Enzyme Bioreactor with Immobilized Lipase (고정화 리파제를 이용한 충진형 효소생물반응기 내에서의 무-트랜스 유지 연속 생산을 위한 에스테르 교환 반응의 최적화)

  • Kim, Sang-Woo;Park, Kyung-Min;Ha, Jae-Uk;Lee, Jae-Hwan;Chang, Pahn-Shick
    • Korean Journal of Food Science and Technology
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    • v.41 no.2
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    • pp.173-178
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    • 2009
  • Epidemiological studies showed that high trans-fat consumption is closely associated with getting the risks of cardiovascular disease. The objective of this study was to produce trans-free fat through lipase-catalyzed interesterification, as a substitute for the cream margarine commonly used in industry. Optimum conditions for interesterification in a packed bed enzyme bioreactor (PBEB) were determined using response surface methodology (RSM) based on central composite design. Three kinds of reaction variables were chosen, such as substrate flow rate (0.4-1.2 mL/min), reaction temperature (60-70$^{\circ}C$), and ratio of fully hydrogenated canola oil (FHCO, 35-45%) to evaluate their effects on the degree of interesterification. Optimum conditions from the standpoint of solid fat content (SFC) were found to be as follows: 0.4 mL/min flow rate, 64.7$^{\circ}C$ reaction temperate, and 42.8% (w/w) ratio of FHCO, respectively. The half-life of immobilized lipase in PBEB with two stages at 60$^{\circ}C$ ($1^{st}$ stage) and 55$^{\circ}C$ ($2^{nd}$ stage) was about more than 30 days as estimated by extrapolating the incubation time course of tristearoyl glycerol (TS) conversion, whereas the half-life of the enzyme in PBEB with single stage at 65$^{\circ}C$ was only about 15 days. Finally, the results from SFC analysis suggest that trans-free fat produced in this study seems to be a suitable substitute for the cream margarine commonly used in industry.

Studies on the Differences of the Rice Tillering System and Yield Characteristics under the Different Cultivation Methods in Southern Region of Korea II. Variations of Heading and Yield Components under Different Nursing Methods and Transplanting Time (남부지방에서 벼 재배형태별 분얼체계 및 수량구성형질의 차이에 관한 연구 II. 출수기와 수량구성형질의 변화)

  • 김용재;신해룡;장강연
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.6
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    • pp.487-498
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    • 1992
  • To study the effects of different nursing methods and transplanting on the growth of rice plant (Oriza sativa L.) in southern region of Korea, Kumo-byeo, Palgong-byeo and Dongjinbyeo were transplanted from April 20 to July 20 at an interval of 15 days with 8 days old seedling (infant seedling) and 25 days old box-seedling for machine transplanting, and 45 days old conventional seedling. Threshold transplanting date in southern region of Korea were June 26 for 8 days old seedling, July 1 for 25 days old seedling and] July 11 for 45 days old seedling for Kumo-byeo, and June 21, June 30, July 10 for Palgong-byeo, June 10, June 24, July 5 for Dongjin-byeo, respectively. Yield has no uniform tendency according to the transplanting date. However, yield were greater in the order of 8 days old seedling >25 days old seedling> 45 days old seedling in Kuma-byeo and 25 days old seedling (equation omitted)8 days old seedling (equation omitted)45 days old seedling in Palgong-byeo, 45 days old seedling(equation omitted)25 days old seedling(equation omitted) 8 days old seedling in Dongjin-byeo. The optimum accumulated air temperature during yield productive stage around heading (40 days from 10 days before heading to 30 days after heading) for high yield were 1,003$^{\circ}C$ for 8 days old seedling, 1,014$^{\circ}C$ for 25 days old seedling and 1,027$^{\circ}C$ for 45 days old seedling in Kumo-byeo. And they were 1,018$^{\circ}C$, 1,015$^{\circ}C$, 1,086$^{\circ}C$ in Palgong-byeo and 998$^{\circ}C$, 984$^{\circ}C$, 949$^{\circ}C$ in Dongjin-byeo, respectively. Earlier transplanting with 8 days old seedling showed higher ratio of broken rice and green kerneled rice in Kuma-byeo, and late transplanting after July 5 showed significant high rate of green kerneled rice. Palgong-byeo and Dongjin-byeo also showed high rate of green kerneled rice at transplanting after July 5.

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The Varietal Comparison in Growth Characteristic and Yield of Vegetable Soybean (풋콩으로의 생육특성과 수량의 품종간 비교)

  • Lee, Seung-Su;Kim, Chang-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.2
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    • pp.156-160
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    • 2008
  • This study was conducted to find on the screening of a optimal variety after compare with characteristics of a colored soybean recommended and a vegetable soybean by pure line isolation from the indigenous variety in Chungnam area. The budding period in results compared with a budding property of testing varieties between the recommended colored soybean 6 varieties and the indigenous 3 varieties, was showed that it was delayed by increasing of a grain weight but the budding rate was not significant. And the harvest period was the most early day at the black colored soybean plot at the 30th July and was the latest day at the native variety (Nonsan jaerae) at the 21 th October. The stem length was showed a wide range from 25 to 104 cm, and the Asan jaerae in this result was the highest on 104 cm, but the Geomjeongolkong was the lowest on 25 cm. The number of pod per plant was searched about 9 to 111 range, and the most numerous plot in this experimental varieties was the Ilpumgeomjeongkong as 111 number, on the other hand, the least variety was the Asan jaerae as 9 number. The rate of more than 2 seed per pod in the vegetable soybean property was the highest at the Geomjeongolkong about 82%. Additionally, the Hwangkuemkong and Geomjeongkong 1 was relatively the high levels as 78% and 73%, respectively. At the pod yield per $1000\;m^2$ (10a) was the most much as 1,567 kg at the Ilpumgeomjeongkong. Also, the Sunheukkong and the Galmikong was 1,468 kg, 1,397 kg, respectively. Therefore, the Ilpumgeomjeongkong, Sunheukkong, and Galmikong were proper varieties for the vegetable cultivation.

Investigation on Characteristics of Summertime Extreme Temperature Events Occurred in South Korea Using Self-Organizing Map (자기조직화지도(Self-Organizing Map)를 이용한 최근 우리나라 여름철 극한온도 특성 분류)

  • Lim, Won-Il;Seo, Kyong-Hwan
    • Atmosphere
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    • v.28 no.3
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    • pp.305-315
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    • 2018
  • This study investigates the characteristic spatial patterns and dynamic processes associated with the summertime extreme temperature events in South Korea during the last 20 years (1995~2014) using Self-Organizing Map (SOM). The classified SOM patterns commonly have high temperature and anticyclonic circulation anomalies over South Korea. The two major teleconnection patterns are identified: one is from the subtropical western North Pacific (WNP) affecting to the north and the other is from the North Atlantic (NA) affecting downstream region. The meridional teleconnection pattern is related to the forcing of positive sea surface temperature (SST) anomaly over the WNP. The northward propagating Rossby wave generates the East Asia-Pacific (EAP) pattern to form an anticyclonic circulation anomaly over South Korea. On the other hand, NA SST anomalies generate an eastward Rossby wave train across the Eurasian continent, leading to the development of an anticyclonic circulation anomaly over South Korea. The EAP pattern occurs more frequently in July and August, whereas the midlatitude teleconnection pattern associated with NA SST anomalies develops more frequently in early summer (June).

Spatial Patterns and Temporal Variability of the Haines Index related to the Wildland Fire Growth Potential over the Korean Peninsula (한반도 산불 확장 잠재도와 관련된 Haines Index의 시.공간적 특징)

  • Choi Cwang-Yong;Kim Jun-Su;Won Myoung-Soo
    • Journal of the Korean Geographical Society
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    • v.41 no.2 s.113
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    • pp.168-187
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    • 2006
  • Windy meteorological conditions and dried fire fuels due to higher atmospheric instability and dryness in the lower troposphere can exacerbate fire controls and result in more losses of forest resources and residential properties due to enhanced large wildland fires. Long-term (1979-2005) climatology of the Haines Index reconstructed in this study reveals that spatial patterns and intra-annual variability of the atmospheric instability and dryness in the lower troposphere affect the frequency of wildland fire incidences over the Korean Peninsula. Exponential regression models verify that daily high Haines Index and its monthly frequency has statistically significant correlations with the frequency of the wildland fire occurrences during the fire season (December-April) in South Korea. According to the climatic maps of the Haines Index created by the Geographic Information System (GIS) using the Digital Elevation Model (DEM), the lowlands below 500m from the mean sea level in the northwestern regions of the Korean Peninsula demonstrates the high frequency of the Haines Index equal to or greater than five in April and May. The annual frequency of the high Haines Index represents an increasing trend across the Korean Peninsula since the mid-1990s, particularly in Gyeongsangbuk-do and along the eastern coastal areas. The composite of synoptic weather maps at 500hPa for extreme events, in which the high Haines Index lasted for several days consecutively, illustrates that the cold low pressure system developed around the Sea of Okhotsk in the extreme event period enhances the pressure gradient and westerly wind speed over the Korean Peninsula. These results demonstrate the need for further consideration of the spatial-temporal characteristics of vertical atmospheric components, such as atmospheric instability and dryness, in the current Korean fire prediction system.

Development of a Value Inquiry Model in Biology Education (생물교육에서의 가치 탐구 모형 개발)

  • Jeong, Eun-Young;Kim, Young-Soo
    • Journal of The Korean Association For Science Education
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    • v.20 no.4
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    • pp.582-598
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    • 2000
  • There are many bioethical issues in line with the rapid advance of biology. In this situation, it is important for students to make a rational decision on value problem. In this study 'value inquiry in biology education' is defined as 'the process of rational value judgement and wise decision-making in the biology-related value problem' and the model was developed. To develop the model, value inquiry models were reviewed. Value clarification model is helpful for the formation of the personal value as the process of individual value inquiry, but it isn't helpful for clarifying the value conflicts. Value analysis model focuses on the rational solution of value problem through the logical procedure. But it has the limitations that overemphasizing the logical and systematic aspects results in devaluating students' affective aspects. So it is necessary to coordinate psychological and logical aspects of value inquiry. In this regard, the model was developed, including identifying and clarifying value problem, understanding biological knowledge related to conflict situation, considering on the related persons, searching for alternatives, predicting the consequences of each alternative, selecting the alternative, evaluating the alternative, and final value judgement and affirming it. The educational objectives of value inquiry were selected in consideration of the ability to carry out the steps of the developed model. And the selected contents were animal duplication, test-tube baby, genetic engineering, growth hormone injection problem, brain death, organ transplant, animal to be experimented and were organized on the basis of the 6th and the 7th science curriculum. And the suitable instructional models for the value inquiry education were selected: bioethical value clarification decision-making model, group presentation according to the value analysis model, role play and debate, and discussion through web forum. And the interview was considered to be suitable to evaluate the students' value inquiry ability and the rubric was made to evaluate the attainment of the educational objectives for value inquiry.

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A Study on the Plant Planning in Landscape Space Considering the Characteristics of the Gender Determination of Pine Tree (소나무 성 결정 요인의 특성을 고려한 조경공간 식재계획)

  • Lee, Chang-Hun
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.38 no.1
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    • pp.45-52
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    • 2020
  • This study analyzed the components contained in the pine needles of first and second-year-olds to analyze the factors that the in vivo content of inorganic elements affects the sex determination of pine trees. In response, the plan for pine tree plant and maintenance was intended to be presented in consideration of the reproductive environment and physiological characteristics. The results are as follows. First, last year, when there were many encyclopedias, the analyzed N(%) content was found to be high. The nitrogen content of the previous year's soil was found to affect the production of the spheres the following year. This is believed to be possible to reduce the rate of Pine pollen produced in the new plant in the following year through a dispute over quality consumption in the spring of the previous year. Second, the weapons elements involved in the Pine cones and the generation of the Pine pollen in the new plant appeared to be P(%), K(%), Ca(%), and Fe(%). However, the nutrients from the previous year's leaves of Ca(%) and Fe(%) were found to have a low influence on the sex determination of first-year pine trees. Because Ca(%) and Fe(%) are not able to move nutrients accumulated in aging organs due to the nature of the components, feeding nutrients in the fall when the growth of the previous year's branches is reduced is expected to affect oral generation. Third, pine trees are extremely positive and Pine pollen is related to the time of the northeast wind. Therefore, it is believed that it would be good to be located in the northern direction, where the sunlight is good, in an outdoor space. In addition, it is important to use vaginal consumer products in spring and carry out a quarrel involving Mg and Fe during fall to reduce the effect of the Pine pollen, which is an outdoor plant. This is an important part of maintaining and managing pine trees in outdoor spaces as well as the sex determination of pine trees. This study suggested that plant planning, which derives the correlation between pine inorganic element content on sexual determination and takes into account the physiological characteristics of pine trees, is an important issue in the creation of outdoor space. Follow-up research on other factors affecting pine tree sex determination is expected.

A Novel Chenodeoxycholic Derivative HS-1200 Enhances Radiation-induced Apoptosis in Human MCF-7 Breast Cancer Cells (담즙산 합성유도체(HS-1200)가 인체 유방암 세포주(MCF-7)에서 유도하는 방사선 감작 효과)

  • Lee Hyung Sik;Choi Young Min;Kwon Hyuk Chan;Song Yeon Suk
    • Radiation Oncology Journal
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    • v.22 no.2
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    • pp.145-154
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    • 2004
  • Purpose : To examine whether a synthetic bile acid derivatives (HS-1200) sensitizes the radiation-induced apoptosis in human breast cancer cells (MCF-7) and to investigate the underlying mechanism. Materials and Methods : Human breast cancer cells (MCF-7) in exponential growth phase were treated with HS-1200 for 24 hours at 37$^{\circ}C$ with 5$\%$ CO$_{2}$ in air atmosphere. After removal of HS-1200, cells were irradiated with 2$\~$8 Gy X-ray, and then cultured Ii drug-free media for 24-96 hours. The effect of radiation on the clonogenicity of MCF-7 cells was determined with clonogenic cell survival assay with 16$\mu$M of HS-1200. The induction of apoptosis was determined using agarose gel electrophoresis and Hoechst staining. The expression level of apoptosis-related molecules, such as PARP, Bax, Bcl-2, Bak and AIF, were assayed by Western blotting analysis with 40$\mu$M of HS-1200 combined with 8 Gy irradiation. To examine the cellular location of cytochrome c, bax and AIF immunofluorescent stainings were undertaken. Results : Treatment of MCF-7 cells with 40$\mu$M of HS-1200 combined with 8 Gy irradiation showed several changes associated with enhanced apoptosis by agarose gel electrophoresis and Hoechst staining. HS-1200 combined with 8 Gy irradiation treatment also enhanced production of PARP cleavage products and increased Bax/Bcl-2 ratio by Western blotting. Loss of mitochondrial membrane potential ($\Delta$$\psi$$_{m}$) and increased cytochrome c staining indicated that cytochrome c had been released from the mitochondria in HS-1200 treated cells. Conclusion : We demonstrated that combination treatment with a synthetic chenodeoxycholic acid derivative HS-1200 and irradiation enhanced radiation-induced apoptosis of human breast cancer cells (MCF-7). We suggest that the increased Bax/Bcl-2 ratio In HS-1200 co-treatment group underlies the increased radio sensitivity of MCF-7 cells. Further futures studies are remained elusive.

The Strategy of Characterizing Space that uses Anti-House as a Metaphor for Character's Self-Defense Mechanism - Focusing on the TV Series and the Theater version of - (캐릭터의 자아방어기제를 은유하는 '안티돔' 공간의 성격화 전략 - <에반게리온>의 TV 시리즈와 극장판 를 중심으로 -)

  • Yang, Se-Hyeok;Ryu, Beom-Yeol
    • Cartoon and Animation Studies
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    • s.41
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    • pp.75-106
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    • 2015
  • Animations characterize space as a strategy to effectively show the inner conflicts of characters and to highlight the theme. During the process of inner conflict, characters unconsciously use defense mechanism to protect their egos from the fear that came from deficiency, and because of the self-deceptive quality of self-defense mechanism, the reality is distorted and conflicts get intensified. This study focuses on the concept of anti-house, the space where conflicts get intensified, analyzes animations to find out the aspect of inner conflict, and interprets the characteristic of space that is used for metaphoric structure frame. Also, it aims to reveal how the defense mechanism, which intensifies the inner conflict of characters, is characterized as anti-house. The analysis in this study was mainly done with the TV series, , and the theater version of . It is because the characters have serious deficiency from broken home and have a psychological quality of closed boundary that is symbolized as 'A.T. field'. Especially, the core character, 'Shinji Ikari', shows how a character uses compulsive self-defense mechanism to deal with inner conflict and as a result, goes through ego-collapse and then introspection. This process of the character's experience is the core of the whole plot. Through analysis, the relationship between the character's self-defense mechanism and the space, anti-house(which expands to Anti-city), was inferred. The space is made up of three axes, x-axis of horizontal space, y-axis of vertical space, and in the sense that all the space has no exit, z-axis of deeper contradictory space. This thesis started with the decision that is the most suitable work in analyzing the metaphorical relationship between self-defense mechanism and anti-house. There was limitation, however, as the typical characteristics of Japanese animations, pedantic composition and the possibility of broad interpretation, hindered clear verification. Hopefully, this limitation will be overcome by following studies and this study is expected to show the importance of space in interpreting the text of animations, and to serve as database for other creative works.

Reproductive Cycle of Small Filefish, Rudarius ercodes (그물코쥐치, Rudarius ercodes의 생식주기)

  • LEE Taek Yuil;HANYU Isao
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.5
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    • pp.423-435
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    • 1984
  • The reproductive cycle of the small filefish, Rudarius ercodes was investigated based on the annual variations of gonadosomatic index(GSI) and hepatosomatic index(HSI) by electronic and photic microscophy. The specimens used were collected at the coastal area of Benden island, Sizuokagen, Japan, from September 1982 to August 1983. GSI began to increase from March, starting season of longer daylength and higher water temperature, and reached the maximum value between June and August. It began to decrease from September with the lowest value appearing between November and February without any evident variation. The annual variations of HSI were not distinct in male filefish and were negatively related to GSI in female : HSI decreased in the summer season when the ovary was getting mature and reached the maximum in the winter season when the ovary was getting retrogressive. The ovary consisted of a pair of saccular structure with numerous ovarian sacs branched toward the median cavity. Oogonia divided and proliferated along the germinal epithelium of the ovarian sac. Young oocytes with basophile cytoplasm showed several scattering nucleoli along the nuclear membrane. when the oocytes growing to about 300 ${\mu}m$, nuclear membrane to disappear with nucleus migrating toward the animal pole. The regions of protoplasm were extremely confined within the animal hemisphere in which most of cytoplasms were filled with yolk materials and oil drops. After ovulation, residual follicles and growing oocytes remaining in the ovarian sacs degenerated. But perinucleatic young oocytes without follicles formed were not degenerated, and growing continuously still in the next year. Mitochondria and endoplasmic reticula in the cytoplasm remarkably increased with oocytes maturing and yolk accumulating. Those were considered to be functionally related to the yolk accumulation. Five or six layers of possible vitellogenin, oval-shaped disc structures with high electron density, appeared in the apex of follicular processes stretching to the microvilli pits of mature oocytes. Testis consisting of a pair of lobular structures in the right and left were united in the posterior seminal vesicle, Cortex of testis was composed of several seminiferous tubules, and medulla consisting of many sperm ducts connected with tubules. Steroid hormone-secreting cells with numerous endoplasmic reticula and large mitochondria of well developed cristae were recognized in the interstitial cells of the growing testis. Axial filament of spermatozoon invaginated deeply in the central cavity of the nucleus and the head formed U-shape with acrosome severely lacking, mitochondria formed large globular paranuclei at the posterior head, and microtubular axoneme of the tail represented 9+9+2 type. The annual reproductive cycles could be divided into five successive stages : growth(March to July), maturation(May to September), Spawning(mid May to early October) and resting stages(October to February). The spawning peak occurred from June to August.

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