• Journal of Practical Agriculture & Fisheries Research
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• v.9 no.1
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• pp.33-46
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• 2007

We conducted this research to develop the medium for some edible mushroom cultivation using shiitake waste log which is abandoned after cultivation of shiitake mushroom because those bed logs can not be recycled. The isolates of P. ostreatus(POS-012), P. eryngii(PER-005), G. frondosa(GFR-001) and F. velutipes(FVE-001) were selected and examined for mycelial growth on sawdust media prepared from shiitake waste log. Mycelial growth of selected isolate were satisfactory on the sawdust extract media using acasia(Robinia pseudo-acacia), neutinamu(Zelkova serrata) and kangchamnamu(sangsuri, Quercus acutissima) which are no shiitake-inoculated. Although the mycelial growth of the isolate were poor on the sawdust media prepared from Quercus spp., sawdust of neutinamu, (Zelkova serrata), beotnamu, (Prunus serrulata), orinamu(Alnus japonica), eunsuweonsasinamu(Populus tomentiglandulosa) and chestnut(Castanea crenata) were excellent for mycelial growth. However, shiitake logs which are infected with harmful fungi such as Hypocrea spp. were useful as recycle materials for mushroom cultivation.

• The Korean Journal of Mycology
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• v.28 no.2
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• pp.103-108
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• 2000

Various conditions for mushroom were investigated to study their effects on the mycelial growth of oyster mushroom and pathogens. Our results show that the optimal pasteurization temperatures for mycelial growth and pathogens are $60^{\circ}C\;and\;121^{\circ}C$ respectively. Both were then decreased by heating over $60^{\circ}C$ and getting down temperature under $121^{\circ}C$, respectively. In the experiment for pasteurization time, it was showed that both 8 and 12 hours pasteurization were greater than 24 hours treatment for the mycelial growth. In contrast, we didn't find any particular effect by different soaking time of substrates. The pH In substrates was leveled well by pasteurization and after-fermentation even though the acidity was lowered by extending the soaking time. Our results also show that the mycelial growth is greatest at $25{\sim}30^{\circ}C$ regardless of pasteurization time for substrates, Through the experiment with paddy straw as a substrate, it was showed that two time pasteurization was greater than one time for mycelium growth and pro-fermentation of paddy straw before pasteurization have resulted in worth mycelial growth compared with that of normal condition.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.1
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• pp.49-54
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• 2018

To select the superior strain of Haesongi, Hypsizigus marmoreus, strains were isolated by Di-Mono mating with isolates from different fruit bodies. Three strains were selected to superior isolates that are good mycelial growth on PDA medium for 10days. When cultured on PDA medium for 10 days, strain No. 3 and strain No. 7 showed mycelial growth of 62mm and 58mm, respectively. Mycelial growth was good in the order of strain No. 2, 10, and 9. The three selected strains, KNCAF-H-3, KNCAF-H-7 and KNCAF-H-2, were cultured in sawdust medium for 10 days and showed mycelial growth of 79mm, 76mm and 73mm respectively. The mycelial growth of the selected three cultivars was better than that of Greenpeace No 5, a control cultivar grown at 55mm.

• The Korean Journal of Mycology
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• v.33 no.1
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• pp.22-29
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• 2005

The main objectives of this research were to study the cultural and nutritional characteristics of Tricholoma matsutake and to establish its liquid culture system. The optimum growth of T. matsutake was observed in HA and TMM agar media. Similarly highest growth was observed in PDB and TMM liquid media. The optimal temperature for the mycelial growth was $25^{\circ}C$. The most suitable carbon source was dextrin among 12 different carbon sources tested. Yeast extract and peptone were best nitrogen sources among 17 different sources tested. The optimum mineral salts were $Fe_{2}(SO_{4})_{3}{\cdot}H_{2}O$ and KCl among 9 different sources tested. Shaking culture gave higher mycelial growth compared to stationary culture. Similarly, optimum medium amount for shaking culture was 100 ml per 250 ml flask. The highest mycelial growth was obtained when $5{\sim}7$ mycelial discs were inoculated in 100 ml of medium and incubated for $8{\sim}9$ weeks, respectively. The highest proportion of mycelial growth was observed at 40 : 1 ratio of medium to inoculum volume in 8 l air-lift fermenter.

• Korean Journal Plant Pathology
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• v.12 no.3
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• pp.331-335
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• 1996

1993년 전북완주와 충남 서산 지역에서 생강 근경 부패병에 감염된 식물체로부터 Pythium sp.를 분리하였으며, 52균주를 형태적 특징과 병원성 검정을 통해 Pythium zingiberum으로 동정하였다. P. zingiberum으로 동정된 균주들이 재배지에서 사용되는 metalaxyl(MT), metalaxyl+copper oxychloride(MC), echlomezol(EM) 및 propamocarb hydrochloride(PC)등에 균사 생장, 난포자 형성에 미치는 영향 및 약제방제 효과 등을 검토하였다. MT, MC, EM 등이 혼합된 평판배지에서의 균사생육 정도는 50, 100 mg/L에서도 균사생장 억제를 나타냈으나 3균주는 EM 100mg/L에서도 균사생장이 저지되지 않았다. 100mg/L에서도 균사생육 억제효과가 적은 PC의 경우 저항성균주의 난포자 형성량은 약제 희석농도가 높아질수록 저하되었으나, 감수성을 나타낸 균주는 10, 50mg/L에서 오히려 증가하였다. 또한 폿트실험을 통한 약효검정 결과에서도 MT, MC, EM등은 대조구에 비해 생강 근경부패병을 억제하였다.

• The Korean Journal of Mycology
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• v.39 no.2
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• pp.91-98
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• 2011

Oudemansiella mucida, an edible and medicinal mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to produce antifungal substances to inhibit the mycelial growth and spore germination of the plant pathogenic fungi. To produce good amount of antifungal substances from culture media, the optimal culture conditions of O. mucida were investigated. The most favorable conditions for the mycelial growth were $25^{\circ}C$ and pH 5 in potato dextrose agar. The most favorable carbon and nitrogen sources promoting mycelial growth were maltose and calcium nitrate, respectively. The optimum C/N ratio was about 20 : 1 in case that 3% glucose was supplemented to the basal medium as a carbon source. The optimal mycelial growth of O. mucida was found in the Hennerberg medium. The crude extract from submerged culture of potato dextrose broth exhibited inhibition of mycelial growth of Colletotrichum acutatum, Botrytis cinerea and Pyricularia oryzae but, fungicidal activity is not good enough to compared with commercially available fungicides tested. Therefore, the antifungal substances extracted from submerged culture of O. mucida might have a potential to be used for biocontrol agent of fungal diseases of plants.

• Journal of Mushroom
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• v.2 no.4
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• pp.207-213
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• 2004

The results of examining characteristics of mycelial growth and culture condition for determining the condition of artificially culturing Fomitopsis pinicola are as follows. 1) Mycelial growth and density of F. pinicola. were the highest in the medium of PIDA(Pine Dextrose Agar;66.3mm/10d) followed by the order of GDA, PDA, CDA, PODA, ODA, YM, MCM, MEA(pH 4.7), CHA, and MEA(pH 4.7). 2) Optimal temperature for the mycelial growth and density of F. pinicola were shown to be $30^{\circ}C$, but the mycelia were dead at $40^{\circ}C$. the mycelial growth and density of KNAC9005 strains was the highest at $30^{\circ}C$(66.3mm/10d) followed by the order of 25, 20, 15, 35, 10, and $5^{\circ}C$. 3) Optimal pH for the mycelial growth and density of $40^{\circ}C$ was revealed to be 6.0(88.4mm/10d). above or below pH 6.0, the mycelial growth and density were shown to be retarded. 4) Optimal carbon, nitrogen and organic acid sources for the spawn growth of $40^{\circ}C$ were maltose(331mg/25ml/15d), peptone(347mg/25ml/15d), and glutamic acid(357mg/25ml/15d), respectively. Optimal level of biotin was 370mg/15d and optimal C/N ratio was 40.

• Korean Journal of Plant Resources
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• v.15 no.2
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• pp.135-143
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• 2002

The results of examining characteristics of mycelial growth and culture condition for determining the condition of artificially culturing Inonotus obliqua (chaga) are as follows. 1) Mycelial growth and density oft, oblique were the highest in the medium of BDA (Birch Dextrose Agar; 66.3mm/10d) followed by the order of GDA, PDA, CDA, PODA, ODA, YM, MCM, MEA (pH 7.0), CHA, and MEA (pH 4.7). 2) Optimal temperature for the mycelial growth and density of 1. obliqua were shown to be 30$^{\circ}C$, but the mycelia were dead at 40$^{\circ}C$. the mycelial growth and density of KNAC3005 strains was the highest at 30$^{\circ}C$(66.3mm/10d) followed by the order of 25, 20, 15, 35, 10, and 5$^{\circ}C$. 3) Optimal pH for the mycelial growth and density were revealed to be 6.0 (88.4mm/10d). Above or below pH 6.0, the mycelial growth and density were shown to be retarded. 4) Optimal carbon, nitrogen and organic acid sources for the spawn growth of 40 were maltose (331mg/25$m\ell$/15d), peptone (347mg/25$m\ell$/15d), and glutamic acid (357mg/25$m\ell$/15d), respectively. Optimal level of biotin was 370mg/15d and optimal C/N ratio was 40.

• The Korean Journal of Mycology
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• v.31 no.1
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• pp.14-21
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• 2003

This study was carried out to obtain the basic data on artificial culture of Lampteromyces japonicus. Favorable media for mycerial growth were glucose peptone medium, malt yeast extract, yeast malt peptone, potato dextrose medium. The optimum conditions for the mycelial growth were $30^{\circ}C$ and pH 6.0. Dextrose as a carbon source was favorable to mycelial growth. The optimal dextrose concentration was 1.2%. As nitrogen sources, yeast extract, $(NH_4)_2HPO_4$ and 0.2% for glutamine. The mineral salts of $Al_2(SO_4)_3{\cdot}14H_2O$ were effective and the optimal concentration was 0.1 M.

• The Korean Journal of Mycology
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• v.22 no.3
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• pp.222-228
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• 1994

In Lentinus edodes(oak mushroom) cultivation, commonly are logs and sawdusts of oak and some other broadleaved tree species used. Recently oak trees have been substantially diminished due to extensive logging. Thus, to develop comparable synthetic formula using other tree species for the cultivation of Lentinus edodes, we investigated the effect of various nutrients and pretreatment on L. edodes mycelial growth in coniferous sawdusts(i.e., Pine and Larch). We found that 1.5 hr pretreatment of sawdust with hot water and adding 10% rice bran, 3% charcoal, 0.02% $NH_4CI$ and 0.5-1% lignosulfornic acid were effective for the growth of L. edodes in pine sawdust media. In larch sawdust pretreatment with acetone for one hr and adding 20% rice bran, 3% charcoal and 0.02% $NH_4CI$ increased L. edodes mycelial growth. We also analyzed the components of oak and coniferous sawdusts and found oak has higher content of xylose and lower content of lignin, arabinose and mannose than conifers. Rice bran, compared with BITEL(HOKKEN Co.) known for better commercial substitute for rice bran, has lower content of xylose and galactose, but the similar C/N ratio.