• Journal of Korean Society of Forest Science
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• v.98 no.4
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• pp.357-362
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• 2009

Trials to find a method for overcoming the depletion of Tricholoma matsutake mycorrhiza from the artificially inoculated pine seedling (Pinus densiflora) when it is transplanted in situ were carried out. The culturing jars containing mycorrhizal seedlings were punched at the bottom to make the jar like a pot with keeping the soil structure of the original medium in vitro, and 8 kinds of irrigation solution were applied. Irrigation of distilled water caused disappearance of T. matsutake mycorrhizae, but biweekly irrigation of 2.5% PDMP (24 g potato dextrose broth, 3 g malt extract, 1 g peptone) solution or 0.5% Tween 80 solution until 3 months of acclimation could keep mycorrhizal association stronger. The percentage of mycorrhizal roots out of total length of roots was increased during the acclimation, and the acclimation rate reached up to 70%. Thus, we recommend that the mycorrhizal pine seedlings inoculated with T. matsutake would be acclimated before transplanting into the field.

• Journal of Korean Society of Forest Science
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• v.82 no.4
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• pp.319-327
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• 1993

The objectives of this study were to isolate ectomycorrhizal fungi associated with roots of Pinus rigida ${\times}$ P. taeda trees planted in Chonbuk and Daejon locations and to identify them, and to observe the morphology of mycorrhizae formed by the isolated fungi. Pinus rigida ${\times}$ P. taeda seedlings formed ectomycorrhizae with Rhizopogon rubescens, Rhizopogon sp. and Suillus pictus isolated from the roots of mature Pinus rigida ${\times}$ P. taeda trees, Pinus rigida ${\times}$ P. taeda seedlings formed ectomycorrhizae with Pisolithus tinetorius too. The root surface of inoculated seedlings was covered with mycelia, and Hartig net were well developed in those roots. The size of vacuole was small, and the protoplasm was planty in ectomycorrhizal cell, compared to nonmycorrhizal cell. But the size of nuclei are not changed even in ectomycorrhizal cell.

• Journal of Korean Society of Forest Science
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• v.64 no.1
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• pp.11-19
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• 1984

Potted, germinating Pinus rigida ${\times}$ P. taeda seedlings were inoculated with Pisolithus tinctorius (Pt) ectomycorrhizal fungus to test the effectiveness of Pt in relation to organic amendment and changes in soil fertility and soil texture. Pt was cultured as mycelia in vermiculite-peat moss mixture with nutrients and added to sterilized pot soils with or without organic amendment (fully fermented compost) at three soil texture levels (sand, loamy sand, and sandy loam) in a factorial design. Plants were grown in a greenhouse for 4 months and harvested to compare their growth with non-mycorrhizal plants and plants infected by natural fungi. Regardless of sod texture, soil fertility, or organic amendment, seedlings inoculated with Pt were better in dry weight and height than non-mycorrhizal plants or those infected by natural fungi. An exception was observed in the most fertile soil (0.075% N and 1.32% organic matter content in sandy loam with organic amendment), where non-mycorrhizal plants were slightly bigger (8%) and heavier (18%) than Pt-inoculated plants. In over-all average, Pt-inoculated seedlings were 30% taller and 107% heavier than those infected by natural fungi and 31 % taller and 60% heavier than non-mycorrhizal plants. Growth stimulation of seedlings by Pt was more pronounced in less fertile sand soil when organic was not amended. Mycorrhizal frequency of Pt (% of mycorrhizal root tips) was reduced to about half (from 84 to 33% in sandy loam and from 77 to 40% in loamy sand) by organic amendment, while that of natural fungi was not significantly affected. Severe nitrogen deficiency was observed in the needles of non-mycorrhizal plants (1.38% N), while both Pt-inoculated plants (1.68% N) and those infected by natural fungi (1.89% N) did not develop symptom, suggesting an active role of mycorrhizae in absorption of soil nitrogen. Top to root ratio increased with organic amendment to non-mycorrhizal plants, but was not significantly affected by fungal treatment. It was concluded from this study that relative effectiveness of Pt was determined by soil fertility. Organic amendment to less fertile sand soil increased effectiveness of Pt, while the same amendment to more fertile loamy sand and sandy loam decreased effectiveness of Pt. Benefits of Pt mycorrhizae would be expected most either when organic was not added to the soil, or when soil nutrients were not abundant.

• Journal of Korean Society of Forest Science
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• v.94 no.3 s.160
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• pp.146-152
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• 2005

For the application of ectomycorrhizal seedlings on damaged slope lands, studies on cultural characteristics of Lyophyllum shimeji and ectomycorrhizal associations of Lespedeza cyrtobotrya seedlings were carried out by artificial inoculation of L. shimeji. Mycelial growth of L. shimeji was best on MP (1% malt extract, 0.1% peptone, 1% glucose and 1.5% agar) medium. An optimum temperature and pH for the mycelial growth were $25^{\circ}C$ and pH6, respectively. Mycorrhizal root of L. cyrtobotrya seedlings inoculated with L. shimeji showed characteristics of ectomycorrhizas with Hartig net. Growth rate of the mycorrhizal seedlings's roots was higher than that of non-mycorrhizal seedlings. When the mycorrhizal seedlings were transplanted in slope land, survival rate and dry weight were 62% and 850 mg/seedling, respectively. On the other hand, survival rate and dry weight of non-mycorrhizal seedlings were 11% and 430 mg/seedling, respectively.

• The Korean Journal of Mycology
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• v.9 no.4
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• pp.225-227
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• 1981

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.3
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• pp.145-153
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• 2003

The average values of soil chemical concentration in investigation regions were pH 5.93, $25.9g\;kg^{-1}$ of organic matter, $742mg\;kg^{-1}$ of available phosphate and $44.7mg\;kg^{-1}$ of nitrate nitrogen. The number of mycorrhizal spores analysed from 1g of soil sample was 12.1 for onion, 11.7 for garlic and 10.1 for red pepper. In fractionation of soil texture, clay and silty clay showed more than 15 spores per 1g of soil. There was no relationship between spore density and soil nutrition of pH, organic matter, $NO_3-N$ and Av. $P_2O_5$. However, the number of spores was constant level independent on the concentration of soil nutrition. Spores identified in this study are as follows: Glomus clarum, Glomus intraradices, Glomus etunicatum, Gigaspora rosea and Gigaspora margarita.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.28-28
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• 2019

본 연구는 멸종위기 아고산수종 구상나무 잔존집단 복원을 위해서 묘목처리 및 입지환경이 구상나무묘목 초기생장에 미치는 영향을 구명하고자 수행하였다. 연구대상지는 경상남도 금원산으로 해발 1,300 m 이상 능선부(1입지)와 구상나무 잔존집단(2입지)을 선정하였다. 식재한 구상나무 실생묘는 지리산 5개 개체에서 종자를 채취하여 2014년도 파종한 1~5묘 1,350본을 이용하였다. 2019년 5월 연구대상지의 조릿대를 제거 후 무처리, 분해포트, 균근균 접종 실생묘를 9개 구역으로 나누어 3반복씩 입지별 0.15 ha, 4,500 본/ha 밀도로 식재하였다. 식재 후 2019년 5월부터 8월까지 입지별 기상데이터를 수집하였고 묘목의 생존율, 수고, 1년지 생장량 및 근원경을 측정하였다. 환경분석 결과 1입지(88.2%, $42.8umole{\cdot}m^{-2}{\cdot}s^{-1}$)가 2입지(84.5%, $29.4umole{\cdot}m^{-2}{\cdot}s^{-1}$)에 비해 대기습도와 광량이 높게 나타났다. 구상나무 실생묘의 생존율은 모든 입지에서 100%로 나타났다. 입지별 생장특성 분석 결과 1입지가 수고 17.3 cm, 1년지 생장 4.72 cm 및 근원경 6.20 mm로 2입지(19.0 cm, 3.88 cm, 5.31 mm)에 비해 양호한 것으로 나타났다. 묘목 처리에 따른 분석 결과 무처리와 분해포트 묘목이 모든 입지에서 초기 생장이 양호하였고 균근균 접종 처리는 초기에는 비교적 낮은 생장특성이 나타났다. 결과적으로 대기습도와 광량이 높은 환경 조건이 구상나무 묘목의 초기 생장에 긍정적인 영향을 주었으며 묘목 처리에 따른 생장특성은 지속적인 모니터링이 필요한 것으로 나타났다.

• The Korean Journal of Mycology
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• v.37 no.1
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• pp.111-113
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• 2009

Ramaria botrytis is one of the commercially important ectomycorrhizal mushrooms in Korea and the mushroom is only picked in natural forest. This study was conducted to make ectomycorrhizal synthesis of the fungus using Pinus densiflora seedling. Ectomycorrhizal types of the fungus were simple and dichotomous. Diameter of ectomycorrhizal root was $325-380{\mu}m$ and fungal mantle was subhyaline with $20-30{\mu}m$ in diameter. On the other hand, optimum temperature for mycelial growth was $25^{\circ}C$ and the fungus grew 5 cm on potato dextrose agar medium in two months.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.6
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• pp.476-481
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• 2007

There was no difference between eco-friendly and conventional cultivated soils in the chemical properties. But $Av.P_2O_5$ contents in the eco-friendly cultivated soils were slightly higher than that of conventional cultivated soils. In the conventional cultivated soils, the coefficient of correlation between spore density and soil chemical properties such as pH, EC, OM, $Av.P_2O_5$, K${\surd}$(Ca+Mg) and CEC was $-0.48^*$, -0.05, $0.48^*$, -0.12, -0.13, 0.31 respectively. But, in the eco-friendly cultivated soils was $-0.68^*$, $0.69^*$, $0.96^{**}$, $0.75^*$, $0.63^*$, $0.92^{**}$ respectively. The spore density was 140 spores $30g^{-1}$ in the eco-friendly cultivated soils and 60 spores $30g^{-1}$ in the conventional cultivated soils. Infection ratio of intercellular hypha was higher than that of arbuscular and vesicular among the fungi structures within the root. Suncheon and Cheonan as eco-friendly cultivated soil were higher than GimJe and NamWon in infection ratio.

• Journal of Korean Society of Forest Science
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• v.95 no.6
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• pp.735-742
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• 2006

The objective of this study was to elucidate the tolerance of woody plants to simulated acid rain in relation to mycorrhizal inoculation. Germinating seedlings of Robinia pseudoacacia were planted in 1I pots with autoclaved soil mixture of vermiculite, sand and nursery soil at 1:1:1 ratio. Each pot was inoculated with both crushed root nodules from a wild tree of the same species and commercial arbuscular mycorrhizal inoculum of Glomus intraradices at the time of planting the seedlings. Simulated acid rains at pH 2.6, 3.6, 4.6, and 5.6 were made by mixing sulfuric acid and nitric acid at 3: 1 ratio. Each pot received nutrient solution without N and P, and was also supplied with 180 ml of the one pH level of the acid rains once a week for 50 days. The plants were grown in the green house. At the end of experimental period, plants were harvested to determine contents of chlorophyll, mineral nutrients and net photosynthesis in the tissues, dry weight of the plants, and mycorrhizal infection in the roots. Mycorrhizal infection rate was significantly reduced only at pH 2.6, which meant vitality of G intraradices was inhibited at extremely low pH. Height growth, dry weight production, nodule production and chlorophyll content were increased by mycorrhizal infection in all the pH levels except pH 3.6. Particularly, mycorrhizal inoculation increased root nodule production by 85% in pH 5.6 and 45% in 4.6 treatments. But the stimulatory effect of mycorrhizal inoculation on nodule production was reduced at pH 3.6 and 2.6. Net photosynthesis was increased by mycorrhizal infection in all the pH levels. The phosphorus(P) content in the tissues was increased by 43% in average by mycorrhizal inoculation, which was statistically significant except in pH 2.6. It was concluded that mycorrhizal inoculation of Robinia pseudoacacia would enhance growth and resistance of the plants to acid rain by improving the photosynthesis, phosphorus nutrition, and more nodule production.